FastQCFastQC Report
Mon 15 Aug 2016
SRR2921597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1571428
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20210.12860913767605006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16680.10614549314381569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38950.047.767281
GGTATCA23750.047.2038731
TCAACGC48400.037.9370964
CAACGCA49500.037.189585
ATCAACG51000.035.8895873
TATCAAC52200.035.29412
AACGCAG52950.035.2221266
TATACCG1901.0622898E-931.3555495
GTACATG76750.030.2241861
TACATGG78350.029.145662
ACGCAGA64500.029.005447
ACATGGG80800.027.8182813
CATGGGG65600.027.7175434
CGCAGAG67400.027.6646238
ATGGGGG37850.027.2300385
GCAGAGT75400.024.7293859
AGAGTAC68300.022.89543510-11
GAGTACT31100.022.77546312-13
AGTACTT37650.020.47318612-13
GTACTTT34450.020.38372614-15
Produced by FastQC (version 0.11.5)