FastQCFastQC Report
Mon 15 Aug 2016
SRR2921596_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921596_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033466
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41900.034.950261
GTACATG103650.034.631981
CATGGGG80900.033.917014
TACATGG107800.032.9073332
ACATGGG109500.032.015953
GGTCTCG8850.030.2504148
ATGGGGG48450.030.2186055
TCAACGC48050.029.8530584
CAACGCA49600.028.7980235
GGTATCA29800.028.3662171
GTCTCGT8650.028.1980559
AACGCAG51450.028.10616
ATCAACG52400.027.1476233
TATCAAC54050.026.5391222
ATGGGGA30800.024.5406555
ACGCAGA60700.023.5225647
GAGTACA76550.022.7851431
AGTACAT75550.021.82282
CGCAGAG67600.021.1215928
TATACCG2055.927626E-520.3225065
Produced by FastQC (version 0.11.5)