##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921596_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2033466
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.830585315908895	31.0	31.0	34.0	26.0	34.0
2	29.877031137968377	31.0	31.0	34.0	26.0	34.0
3	30.024753302981214	31.0	31.0	34.0	26.0	34.0
4	33.471619884473114	37.0	35.0	37.0	28.0	37.0
5	33.51977215257103	37.0	35.0	37.0	28.0	37.0
6	33.35513305853159	37.0	35.0	37.0	27.0	37.0
7	33.257576964650504	37.0	35.0	37.0	27.0	37.0
8	33.3076933668918	37.0	35.0	37.0	27.0	37.0
9	34.96035734061942	39.0	37.0	39.0	27.0	39.0
10-11	35.00122967386718	39.0	37.0	39.0	27.0	39.0
12-13	34.94578517663929	39.0	37.0	39.0	27.0	39.0
14-15	36.212374094280406	40.0	38.0	41.0	27.0	41.0
16-17	36.17205057768362	40.0	38.0	41.0	27.0	41.0
18-19	36.119553265213185	40.0	38.0	41.0	27.0	41.0
20-21	36.16483604840209	40.0	38.0	41.0	27.0	41.0
22-23	36.07679744829764	40.0	38.0	41.0	26.5	41.0
24-25	36.06958832849922	40.0	37.5	41.0	26.5	41.0
26-27	36.033564613325225	40.0	37.5	41.0	26.5	41.0
28-29	35.97292701230313	40.0	37.0	41.0	26.0	41.0
30-31	35.87298828699373	40.0	37.0	41.0	25.0	41.0
32-33	35.78439816549675	40.0	37.0	41.0	25.0	41.0
34-35	35.66017331983913	40.0	37.0	41.0	24.5	41.0
36-37	35.550162874619005	40.0	37.0	41.0	24.0	41.0
38-39	35.43779291121662	40.0	36.0	41.0	24.0	41.0
40-41	35.3286354431301	40.0	36.0	41.0	23.0	41.0
42-43	35.04847118171634	40.0	35.5	41.0	21.0	41.0
44-45	34.639941361202986	39.5	35.0	41.0	18.5	41.0
46-47	34.363325720715274	39.0	35.0	41.0	16.0	41.0
48-49	34.160508953678104	39.0	34.5	41.0	15.0	41.0
50-51	33.95142333336284	39.0	34.0	40.0	13.0	41.0
52-53	33.74342772389605	38.0	34.0	40.0	9.5	41.0
54-55	33.500144826616236	38.0	33.5	40.0	8.0	41.0
56-57	33.24786227062562	38.0	33.0	40.0	7.5	41.0
58-59	33.262886126446176	38.0	33.0	40.0	7.0	41.0
60-61	33.05458537295436	37.0	33.0	40.0	3.5	41.0
62-63	32.75519212025183	37.0	33.0	40.0	2.0	41.0
64-65	32.46052134631216	36.0	33.0	39.5	2.0	41.0
66-67	32.13459236594071	36.0	32.5	39.0	2.0	41.0
68-69	31.805483838923294	35.0	32.0	39.0	2.0	40.5
70-71	31.44698190183657	35.0	32.0	38.0	2.0	40.0
72-73	31.101474772629587	35.0	32.0	37.0	2.0	39.5
74-75	30.74581896132023	35.0	31.0	37.0	2.0	39.0
76-77	30.45484188080843	35.0	31.0	36.0	2.0	39.0
78-79	30.18704345191904	35.0	31.0	36.0	2.0	37.5
80-81	29.929178801120845	35.0	31.0	35.0	2.0	37.0
82-83	29.708777033891888	35.0	31.0	35.0	2.0	36.5
84-85	29.493780323841165	34.5	30.5	35.0	2.0	36.0
86-87	29.32912721432274	34.0	30.0	35.0	2.0	36.0
88-89	29.169662290886592	34.0	30.0	35.0	2.0	35.5
90-91	28.986936835924475	34.0	30.0	35.0	2.0	35.0
92-93	28.9103156875994	34.0	30.0	35.0	2.0	35.0
94-95	28.792837696819127	34.0	30.0	35.0	2.0	35.0
96-97	28.709464038247994	34.0	29.5	35.0	2.0	35.0
98-99	28.621800659563526	34.0	29.0	35.0	2.0	35.0
100-101	28.511796607368897	34.0	29.0	35.0	2.0	35.0
102-103	27.621539529060236	32.5	28.0	34.5	2.0	35.0
104-105	28.55344618498662	34.0	29.5	35.0	2.0	35.0
106-107	28.843044584959866	34.0	30.0	35.0	2.0	35.0
108-109	28.854024606263394	34.0	30.0	35.0	2.0	35.0
110-111	28.793895988425675	34.0	30.0	35.0	2.0	35.0
112-113	28.718394603106223	34.0	30.0	35.0	2.0	35.0
114-115	28.609582358396942	34.0	30.0	35.0	2.0	35.0
116-117	28.491999374467042	34.0	30.0	35.0	2.0	35.0
118-119	28.209388797255524	34.0	29.5	35.0	2.0	35.0
120-121	28.084179917441453	34.0	29.0	35.0	2.0	35.0
122-123	27.84325678422949	34.0	29.0	35.0	2.0	35.0
124-125	27.062527723600986	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	103232.0
3	17456.0
4	8070.0
5	6912.0
6	7045.0
7	7667.0
8	7777.0
9	8290.0
10	8755.0
11	9055.0
12	9531.0
13	9304.0
14	9445.0
15	8144.0
16	6996.0
17	6426.0
18	5936.0
19	5790.0
20	5609.0
21	5901.0
22	6565.0
23	8576.0
24	12161.0
25	15148.0
26	17444.0
27	19957.0
28	23811.0
29	28741.0
30	34489.0
31	43778.0
32	57166.0
33	79376.0
34	121156.0
35	201024.0
36	392316.0
37	568667.0
38	145692.0
39	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.64340701665672	17.80648068259963	12.02965762784232	25.520454672901334
2	16.189325318543155	19.083178039396216	38.610818884726406	26.116677757334227
3	19.69505159689209	23.206453472302144	28.79520691381632	28.30328801698945
4	13.289387032218054	14.741798676889436	34.81572997566936	37.153084315223154
5	14.407624571408054	37.164965415070796	33.85038987049895	14.577020143022196
6	34.29579094001191	33.933854112828996	16.357989141402953	15.41236580575614
7	29.893197457996166	31.060373821198617	20.599235824244495	18.447192896560715
8	28.247930427937067	34.69973061736595	18.76256196415612	18.289776990540858
9	27.606880617285956	15.67588945638716	18.342672213425264	38.37455771290163
10-11	26.447068640906945	25.28106403701418	26.623287962741777	21.648579359337102
12-13	26.193676074982903	22.630864356316096	26.982168604063528	24.193290964637477
14-15	23.919622857674128	24.0870219465961	24.026221102074338	27.96713409365544
16-17	24.574555406248702	27.173552369194777	24.54420208522888	23.707690139327646
18-19	24.44881596318806	26.517322751163963	25.817898884754342	23.215962400893634
20-21	24.87789148391178	25.527874291072074	25.89866407454068	23.695570150475472
22-23	25.218100804807097	25.063549701598053	25.4918552577139	24.226494235880946
24-25	24.70514850676627	25.553361540690368	25.520746476663014	24.22074347588035
26-27	24.350283305875635	25.76072418457772	25.728153787378776	24.16083872216787
28-29	24.52433572805465	25.611603492403052	25.84057965310161	24.023481126440696
30-31	24.80417272939239	25.537455101396322	25.618570190376964	24.039801978834323
32-33	24.2860979849885	25.844581133211538	25.649755367116576	24.21956551468338
34-35	24.422951369738666	26.02132011113183	25.628757304280704	23.926971214848802
36-37	24.741725261237974	25.70550175420994	25.538035998006148	24.014736986545934
38-39	24.604032071428552	25.89673661089672	25.47836978846049	24.020861529214233
40-41	24.49458976183735	25.79876870865941	25.57690798622343	24.129733543279812
42-43	24.64110084297124	25.58262163441733	25.55409219190969	24.222185330701738
44-45	24.43602929802251	25.60827482475709	25.740683038493067	24.215012838727336
46-47	24.351140363325488	25.63136061246339	25.848997808871303	24.16850121533982
48-49	24.35703252592432	25.841015525078216	25.63743334448358	24.164518604513884
50-51	24.25366599930148	25.958152759399272	25.583436225631743	24.204745015667505
52-53	24.467091894582698	25.778069198981377	25.54077844549962	24.214060460936302
54-55	24.334689880202983	25.621962433973174	25.643138239643715	24.400209446180128
56-57	24.325385240291116	25.757906301749774	25.532319405662644	24.384389052296463
58-59	24.350070053654925	25.675543736622853	25.67342872369545	24.30095748602677
60-61	24.536148904563742	25.81240346683194	25.63815187557182	24.013295753032494
62-63	24.33946444904686	25.899407793592736	25.57028672785239	24.190841029508007
64-65	24.266700507664147	25.691244182963715	25.638293843151892	24.403761466220242
66-67	24.332544629022024	25.791836172504883	25.550748449236792	24.3248707492363
68-69	24.300999422520896	25.761126310518485	25.59422796356471	24.34364630339591
70-71	24.20472685398502	25.72630613578838	25.72320649487932	24.34576051534728
72-73	24.356702234775092	25.867818067969473	25.455988462376556	24.319491234878882
74-75	24.27547841380486	25.9636086069852	25.484819787773844	24.2760931914361
76-77	24.3163303826248	25.71343902436025	25.53961814987009	24.43061244314486
78-79	24.296161151789736	25.74824496831068	25.59715840183222	24.35843547806736
80-81	24.32608307506307	26.006098145815432	25.41106411611562	24.25675466300588
82-83	24.385791021869803	25.826979645242847	25.57483250858363	24.212396824303717
84-85	24.35834474597537	25.865944717153784	25.38753594398186	24.388174592888987
86-87	24.240195957876924	25.924799197280983	25.5486722181507	24.28633262669139
88-89	24.22631641986625	25.74489586866212	25.58601623080932	24.442771480662305
90-91	24.317675292779732	25.73131603191913	25.59604882015019	24.354959855150952
92-93	24.384776414562396	25.803992562849015	25.596055290265042	24.215175732323548
94-95	24.364398704021344	25.64166313162053	25.6721567470198	24.321781417338325
96-97	24.35507107018682	25.53866425724658	25.655159456031118	24.45110521653548
98-99	24.263184530059593	25.67197049541364	25.44323027810306	24.621614696423716
100-101	24.219603584515497	25.72522599951654	25.641074823478032	24.414095592489936
102-103	24.062730066554245	25.637891411291253	25.75120034189396	24.548178180260543
104-105	24.022916602118745	25.81275053814524	25.57282898907382	24.591503870662194
106-107	24.056362409159814	25.682221940817257	25.708704060468733	24.552711589554193
108-109	24.03004197909161	25.750692888638593	25.650454589050025	24.568810543219772
110-111	23.908568281180077	25.97212591532534	25.670861549205036	24.44844425428955
112-113	23.90657757490008	25.999073477101753	25.691904579944318	24.402444368053843
114-115	23.910160327861192	26.107656611387842	25.64603926612408	24.33614379462688
116-117	23.834960588098603	26.038215589432617	25.634813784759746	24.49201003770903
118-119	23.871787322830258	25.87867956545732	25.687584544136044	24.561948567576376
120-121	23.794893433075618	26.075412582763057	25.73581572708914	24.39387825707219
122-123	23.786992287728108	26.159490225343905	25.581039097394942	24.472478389533048
124-125	23.740277141248896	26.15955280893001	25.65174664895294	24.44842340086816
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	752.0
1	595.0
2	376.5
3	276.5
4	200.5
5	148.5
6	157.5
7	216.0
8	290.5
9	320.5
10	291.5
11	307.0
12	332.0
13	364.5
14	427.0
15	462.0
16	506.5
17	559.0
18	608.0
19	668.5
20	753.0
21	888.0
22	1083.0
23	1285.0
24	1506.0
25	1892.0
26	2570.0
27	3492.5
28	4670.0
29	6325.5
30	8703.0
31	11357.0
32	14845.5
33	18678.5
34	22603.5
35	27004.0
36	31957.0
37	36926.5
38	41033.0
39	45489.0
40	49628.0
41	53393.0
42	57115.0
43	61719.0
44	67132.0
45	71978.5
46	76933.5
47	81348.0
48	85511.0
49	88819.5
50	91395.5
51	92774.0
52	90103.5
53	87485.5
54	86341.0
55	83383.5
56	77832.0
57	70730.5
58	64382.0
59	57631.0
60	50034.5
61	42999.0
62	36199.0
63	28460.5
64	20976.5
65	15830.0
66	12231.5
67	9445.0
68	7242.5
69	5527.0
70	4368.5
71	3372.0
72	2542.0
73	2011.5
74	1547.5
75	1156.0
76	864.5
77	567.0
78	392.0
79	294.5
80	211.5
81	156.5
82	123.0
83	103.5
84	90.0
85	86.5
86	66.0
87	54.0
88	54.5
89	47.0
90	38.0
91	30.5
92	30.5
93	31.5
94	22.0
95	16.5
96	21.0
97	17.5
98	10.0
99	6.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026162227448110763
2	0.011064851834257372
3	0.010032132329726684
4	0.011163206072784104
5	0.017457877338494965
6	0.09323981812334212
7	0.05955349142793634
8	0.0511933811531641
9	0.027883426622328577
10-11	0.05190644938248291
12-13	0.013892536191900923
14-15	0.029481682998387975
16-17	0.036366479695259225
18-19	0.04251361960317999
20-21	0.0316208876863444
22-23	0.03958758100700971
24-25	0.03260443007161172
26-27	0.04649696626351264
28-29	0.04622649210756413
30-31	0.027145769833378085
32-33	0.030686522420340444
34-35	0.04548883531861364
36-37	0.010548492081992027
38-39	0.02171169815477613
40-41	0.04349716198844731
42-43	0.023531251567520676
44-45	0.01767917437518011
46-47	0.0243672625949979
48-49	0.03078487665886718
50-51	0.029309563080966192
52-53	0.025424570659160272
54-55	0.02387549140236424
56-57	0.02687529567742957
58-59	0.018761070998974165
60-61	0.02390007996199592
62-63	0.023383720209730577
64-65	0.021096984163984055
66-67	0.029309563080966192
68-69	0.024195142677576116
70-71	0.04782474848362353
72-73	0.022867360457465236
74-75	0.010351783604938563
76-77	0.016646454870649422
78-79	0.025842576172898882
80-81	0.01912989939344941
82-83	0.025867164732530566
84-85	0.013154879402950431
86-87	0.018490596843025654
88-89	0.023629605806047407
90-91	0.02227723502630484
92-93	0.00789292764177026
94-95	0.012466399733263304
96-97	0.016474334953227643
98-99	0.007622453485821744
100-101	0.010843554797572224
102-103	0.00427840937591285
104-105	0.004573472091493047
106-107	9.097767063722728E-4
108-109	0.015294084090906854
110-111	0.0017703762934811796
112-113	0.0028030957980118673
114-115	0.014187598907481119
116-117	0.006565145421659374
118-119	0.27927686029665605
120-121	0.0012540165412158355
122-123	9.097767063722728E-4
124-125	0.3153728658359668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	2033466.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.60763799984078
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.74834935825223	51.180710637059725
2	9.893538867584043	12.388221999181129
3	3.218611604954619	6.045290106752559
4	1.538637316472225	3.853217924909581
5	0.8814413808691878	2.759248144576895
6	0.5671525047695725	2.130484722558982
7	0.3933574030656247	1.7239024526983064
8	0.27724353845401695	1.3886050474659224
9	0.22028030361154038	1.2412106556305706
>10	1.1894060684052716	13.29383117715264
>50	0.05660163874053188	2.3565811304515605
>100	0.014831269241786838	1.3749917361132482
>500	3.9196112809412957E-4	0.1589685679860299
>1k	1.5678445123764096E-4	0.10473569746284937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.83542385267322E-5
2	0.0	0.0	0.0	4.91771192633661E-5	9.83542385267322E-5
3	0.0	0.0	0.0	4.91771192633661E-5	9.83542385267322E-5
4	0.0	0.0	0.0	4.91771192633661E-5	9.83542385267322E-5
5	0.0	0.0	0.0	4.91771192633661E-5	9.83542385267322E-5
6	0.0	0.0	0.0	4.91771192633661E-5	9.83542385267322E-5
7	0.0	0.0	0.0	4.91771192633661E-5	9.83542385267322E-5
8	0.0	0.0	0.0	4.91771192633661E-5	9.83542385267322E-5
9	0.0	0.0	0.0	4.91771192633661E-5	2.458855963168305E-4
10-11	0.0	0.0	0.0	4.91771192633661E-5	2.458855963168305E-4
12-13	0.0	0.0	0.0	4.91771192633661E-5	3.196512752118796E-4
14-15	0.0	0.0	0.0	4.91771192633661E-5	4.180055137386118E-4
16-17	0.0	0.0	0.0	9.83542385267322E-5	4.91771192633661E-4
18-19	0.0	0.0	0.0	1.4753135779009828E-4	4.91771192633661E-4
20-21	0.0	0.0	0.0	1.4753135779009828E-4	5.409483118970271E-4
22-23	0.0	0.0	0.0	3.934169541069287E-4	6.147139907920762E-4
24-25	0.0	0.0	0.0	8.360110274772236E-4	6.884796696871253E-4
26-27	0.0	4.91771192633661E-5	0.0	0.0013523707797425677	7.376567889504915E-4
28-29	0.0	4.91771192633661E-5	0.0	0.0033686326695405774	7.376567889504915E-4
30-31	0.0	4.91771192633661E-5	0.0	0.012048394219524694	7.622453485821746E-4
32-33	0.0	4.91771192633661E-5	0.0	0.027490009668221647	8.851881467405897E-4
34-35	0.0	4.91771192633661E-5	0.0	0.04870993663036412	9.343652660039558E-4
36-37	0.0	4.91771192633661E-5	0.0	0.08165860653681939	9.343652660039558E-4
38-39	0.0	4.91771192633661E-5	0.0	0.1353354322127835	9.343652660039558E-4
40-41	0.0	4.91771192633661E-5	0.0	0.19749531096167822	9.83542385267322E-4
42-43	0.0	4.91771192633661E-5	0.0	0.25564725449060866	9.83542385267322E-4
44-45	0.0	4.91771192633661E-5	0.0	0.3211020002301489	0.0010081309448990048
46-47	0.0	4.91771192633661E-5	0.0	0.3936382511436139	0.001032719504530688
48-49	0.0	4.91771192633661E-5	0.0	0.46996114024035807	0.0010818966237940541
50-51	0.0	4.91771192633661E-5	0.0	0.550882089988227	0.0010818966237940541
52-53	0.0	4.91771192633661E-5	0.0	0.6299097206444563	0.0012048394219524691
54-55	0.0	4.91771192633661E-5	0.0	0.7120600983739094	0.0012786051008475184
56-57	0.0	4.91771192633661E-5	0.0	0.8054474478550415	0.0012786051008475184
58-59	0.0	4.91771192633661E-5	0.0	0.9084489241521618	0.0012786051008475184
60-61	0.0	4.91771192633661E-5	0.0	1.014401027605084	0.0012786051008475184
62-63	0.0	4.91771192633661E-5	0.0	1.1275329904704579	0.0014261364586376168
64-65	0.0	4.91771192633661E-5	0.0	1.2367307837947623	0.0014261364586376168
66-67	0.0	4.91771192633661E-5	0.0	1.3662633159344686	0.0014261364586376168
68-69	0.0	4.91771192633661E-5	0.0	1.5041805469085787	0.0014507250182692998
70-71	0.0	4.91771192633661E-5	0.0	1.6562607882305382	0.0016474334953227642
72-73	0.0	4.91771192633661E-5	0.0	1.812201433414672	0.001967084770534644
74-75	0.0	4.91771192633661E-5	0.0	1.9717811854242953	0.00201626188979801
76-77	0.0	4.91771192633661E-5	0.0	2.1467041986440885	0.00201626188979801
78-79	0.0	4.91771192633661E-5	0.0	2.3418144193214934	0.002040850449429693
80-81	0.0	4.91771192633661E-5	0.0	2.545751932906673	0.002188381807219791
82-83	0.0	4.91771192633661E-5	0.0	2.7581970881244144	0.0023605017246415725
84-85	0.0	4.91771192633661E-5	0.0	2.9829365231579974	0.0024096788439049387
86-87	0.0	4.91771192633661E-5	0.0	3.21657701677825	0.0024096788439049387
88-89	0.0	4.91771192633661E-5	0.0	3.4726914539018603	0.002434267403536622
90-91	0.0	4.91771192633661E-5	0.0	3.75494352991395	0.002458855963168305
92-93	0.0	4.91771192633661E-5	0.0	4.049366942943722	0.0025080330824316707
94-95	0.0	7.376567889504914E-5	0.0	4.348511359422779	0.002557210201695037
96-97	0.0	9.83542385267322E-5	0.0	4.6690724113410305	0.002655564440221769
98-99	0.0	9.83542385267322E-5	0.0	5.013164714826804	0.002655564440221769
100-101	0.0	9.83542385267322E-5	0.0	5.3916564132373	0.002655564440221769
102-103	0.0	9.83542385267322E-5	0.0	5.792326992435576	0.002753918678748501
104-105	0.0	9.83542385267322E-5	0.0	6.205414794247851	0.0028030957980118673
106-107	0.0	9.83542385267322E-5	0.0	6.632616429288712	0.0028276843576435506
108-109	0.0	9.83542385267322E-5	0.0	7.08563605194284	0.0028522729172752335
110-111	0.0	9.83542385267322E-5	0.0	7.573694372072117	0.0028768614769069164
112-113	0.0	9.83542385267322E-5	0.0	8.091898266309837	0.0029014500365385997
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4190	0.0	34.95026	1
GTACATG	10365	0.0	34.63198	1
CATGGGG	8090	0.0	33.91701	4
TACATGG	10780	0.0	32.907333	2
ACATGGG	10950	0.0	32.01595	3
GGTCTCG	885	0.0	30.250414	8
ATGGGGG	4845	0.0	30.218605	5
TCAACGC	4805	0.0	29.853058	4
CAACGCA	4960	0.0	28.798023	5
GGTATCA	2980	0.0	28.366217	1
GTCTCGT	865	0.0	28.198055	9
AACGCAG	5145	0.0	28.1061	6
ATCAACG	5240	0.0	27.147623	3
TATCAAC	5405	0.0	26.539122	2
ATGGGGA	3080	0.0	24.540655	5
ACGCAGA	6070	0.0	23.522564	7
GAGTACA	7655	0.0	22.785143	1
AGTACAT	7555	0.0	21.8228	2
CGCAGAG	6760	0.0	21.121592	8
TATACCG	205	5.927626E-5	20.322506	5
>>END_MODULE