FastQCFastQC Report
Mon 15 Aug 2016
SRR2921596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2033466
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23650.11630388705786082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58800.035.9051
GGTATCA40550.034.8567161
GTACATG110400.033.168691
TACATGG113900.031.7790132
CATGGGG84250.031.77754
ACATGGG114150.030.4556663
TCAACGC70350.029.5804424
CAACGCA72050.028.7706685
ATGGGGG48000.028.170165
ATCAACG74200.027.886953
TATCAAC75350.027.6196162
AACGCAG75650.027.4917896
ATGGGGA31800.023.6019635
ACGCAGA87800.023.2795937
TATCACG2052.2215117E-623.2708152
CGCAGAG92850.022.2054358
TATCTCG2454.3022737E-721.8817025
GAGTACT37000.021.3203512-13
TATACAG9250.020.6069135
GCAGAGT101650.020.2830779
Produced by FastQC (version 0.11.5)