FastQCFastQC Report
Mon 15 Aug 2016
SRR2921595_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921595_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690950
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG77500.036.1742741
TACATGG80250.034.263062
ACATGGG81800.033.394563
CATGGGG68150.032.83514
GTATCAA30750.032.1324351
GGTATCA21750.030.3769651
TCAACGC32350.028.147094
CAACGCA32900.027.855795
ATGGGGG40550.027.4436195
AACGCAG34350.026.5035426
ATCAACG35800.025.9333083
TATCAAC37500.025.3932292
GAGTACA57000.022.8691431
ATGGGGA28850.022.0713355
AGTACAT55850.022.058522
ACGCAGA41950.021.8386177
GTATACG1450.00385968220.5249751
ATGCCTA9950.020.3369122
GTGTACG4152.910383E-1120.0798551
GTAGTAC3602.1755113E-919.8378723
Produced by FastQC (version 0.11.5)