FastQCFastQC Report
Mon 15 Aug 2016
SRR2921595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1690950
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24420.14441586090659098No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20240.11969602885951683No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23450.046.0457841
GTATCAA39250.045.900921
TCAACGC48950.036.54734
CAACGCA49400.036.0551835
GTACATG79450.034.689831
AACGCAG51800.034.513986
TATCAAC53050.033.7307172
ATCAACG53400.033.396953
TACATGG82900.032.8814852
ACATGGG82750.032.147243
CATGGGG72350.028.5183454
ACGCAGA63000.028.376487
ATGGGGG39700.027.158865
CGCAGAG68300.026.260978
GCAGAGT75300.023.819719
AGTACAT57750.023.2391512
GAGTACA60050.022.849091
GAGTACT34900.021.75023312-13
AGAGTAC68550.021.42764510-11
GTACTTT36250.020.68774414-15
Produced by FastQC (version 0.11.5)