##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921595_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1690950
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.903199384961116	33.0	31.0	34.0	30.0	34.0
2	32.07404713326828	34.0	31.0	34.0	30.0	34.0
3	32.148143942754075	34.0	31.0	34.0	30.0	34.0
4	35.55088027440196	37.0	35.0	37.0	35.0	37.0
5	35.41962742836867	37.0	35.0	37.0	33.0	37.0
6	35.45203938614388	37.0	36.0	37.0	33.0	37.0
7	35.34415624353174	37.0	36.0	37.0	33.0	37.0
8	35.39039297436352	37.0	36.0	37.0	33.0	37.0
9	37.13937372482924	39.0	38.0	39.0	35.0	39.0
10-11	37.10018421597327	39.0	38.0	39.0	34.5	39.0
12-13	37.035681421686036	39.0	38.0	39.0	34.0	39.0
14-15	38.49089890298353	41.0	39.0	41.0	34.0	41.0
16-17	38.49441822644076	41.0	39.0	41.0	34.5	41.0
18-19	38.50296608415388	41.0	39.0	41.0	34.0	41.0
20-21	38.4391658535143	41.0	39.0	41.0	34.0	41.0
22-23	38.446313906383985	41.0	39.0	41.0	34.0	41.0
24-25	38.40906797953813	41.0	39.0	41.0	34.0	41.0
26-27	38.340336497235285	41.0	39.0	41.0	34.0	41.0
28-29	38.27724474407877	41.0	39.0	41.0	34.0	41.0
30-31	38.16942251397144	41.0	39.0	41.0	34.0	41.0
32-33	38.05713740796594	41.0	38.0	41.0	34.0	41.0
34-35	37.92641444158609	41.0	38.0	41.0	33.0	41.0
36-37	37.85251811112097	41.0	38.0	41.0	33.0	41.0
38-39	37.774953132854314	40.5	38.0	41.0	33.0	41.0
40-41	37.65965670185399	40.0	38.0	41.0	33.0	41.0
42-43	37.50325201809633	40.0	38.0	41.0	33.0	41.0
44-45	37.34897306247967	40.0	38.0	41.0	32.0	41.0
46-47	37.15086046305331	40.0	37.0	41.0	32.0	41.0
48-49	36.964768029805725	40.0	37.0	41.0	31.0	41.0
50-51	36.72000946213667	40.0	36.5	41.0	31.0	41.0
52-53	36.592014548035124	40.0	36.0	41.0	31.0	41.0
54-55	36.46095153611875	40.0	36.0	41.0	31.0	41.0
56-57	36.26109790354535	39.0	35.0	41.0	30.0	41.0
58-59	36.06742008929892	39.0	35.0	41.0	30.0	41.0
60-61	35.81342144948106	39.0	35.0	41.0	30.0	41.0
62-63	35.460342706762475	38.0	35.0	41.0	29.5	41.0
64-65	35.16947544279843	37.0	35.0	40.0	29.0	41.0
66-67	34.86209882018983	37.0	35.0	39.5	29.0	41.0
68-69	34.49293592359325	36.0	35.0	39.0	29.0	41.0
70-71	34.12186699784145	36.0	35.0	39.0	29.0	41.0
72-73	33.80049705786688	35.0	35.0	37.5	29.0	40.0
74-75	33.48156746207753	35.0	34.0	37.0	28.5	39.0
76-77	33.15809012685177	35.0	34.0	37.0	28.0	39.0
78-79	32.898561163842814	35.0	34.0	36.0	28.0	38.5
80-81	32.64789497028298	35.0	34.0	36.0	27.0	37.0
82-83	32.430239214642654	35.0	34.0	35.0	27.0	37.0
84-85	32.222708832313195	35.0	34.0	35.0	26.5	36.5
86-87	32.09894556314498	35.0	34.0	35.0	26.0	36.0
88-89	32.00369348591029	35.0	34.0	35.0	26.0	36.0
90-91	31.897884916762766	35.0	34.0	35.0	26.0	36.0
92-93	31.822840710843018	35.0	34.0	35.0	26.0	35.0
94-95	31.757183240190425	35.0	34.0	35.0	25.0	35.0
96-97	31.69292675714835	35.0	34.0	35.0	25.0	35.0
98-99	31.617099855111032	35.0	33.5	35.0	25.0	35.0
100-101	31.553941866997842	35.0	33.0	35.0	25.0	35.0
102-103	30.86699813714184	34.0	32.0	35.0	23.5	35.0
104-105	31.38806972411958	35.0	33.0	35.0	24.5	35.0
106-107	31.486074100357786	35.0	33.0	35.0	25.0	35.0
108-109	31.47480735681126	35.0	33.0	35.0	25.0	35.0
110-111	31.40833022856974	35.0	33.0	35.0	24.0	35.0
112-113	31.377110795706557	35.0	33.0	35.0	24.0	35.0
114-115	31.25506135604246	35.0	33.0	35.0	24.0	35.0
116-117	31.443378574174282	35.0	34.0	35.0	25.0	35.0
118-119	31.43020077471244	35.0	34.0	35.0	24.5	35.0
120-121	31.353241668884355	35.0	34.0	35.0	24.0	35.0
122-123	31.221212631952454	35.0	34.0	35.0	23.5	35.0
124-125	30.588075342263224	34.5	32.5	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	21907.0
3	6066.0
4	3339.0
5	2632.0
6	2691.0
7	3192.0
8	3574.0
9	3776.0
10	4185.0
11	4551.0
12	5067.0
13	6612.0
14	8847.0
15	8027.0
16	4559.0
17	3971.0
18	3690.0
19	3194.0
20	3166.0
21	3272.0
22	3732.0
23	4594.0
24	5762.0
25	7627.0
26	9363.0
27	11149.0
28	13664.0
29	16725.0
30	20557.0
31	25696.0
32	33595.0
33	45921.0
34	70878.0
35	127792.0
36	305100.0
37	644699.0
38	237416.0
39	362.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.403188139006076	17.65928272787717	10.591559026947985	27.345970106168764
2	16.532892861493842	19.627550543670463	36.12862629323549	27.7109303016002
3	19.401538862883612	22.457850454518834	28.331072619491987	29.809538063105563
4	13.600114678423575	14.375437966732596	33.8037162680466	38.22073108679723
5	14.787745572696853	37.06092965232827	32.91894126839817	15.232383506576703
6	35.84658002489062	33.322187983972	15.758616587948937	15.072615403188438
7	31.171431512952598	31.12699590435043	18.721946693836884	18.979625888860095
8	28.959107894668335	34.01376401901782	18.058826035200486	18.968302051113355
9	27.831539267907722	14.891137419338046	18.089042279050727	39.188281033703504
10-11	27.318900548826115	24.65089966473941	25.867909516625296	22.16229026980918
12-13	26.975079407908126	21.901996913624078	26.68102754543174	24.441896133036057
14-15	24.239475469513188	23.299310016725165	23.767218146373732	28.693996367387914
16-17	24.56081701746969	26.69433172090266	24.52164770278182	24.223203558845828
18-19	24.58969404682179	25.666321959576983	26.455697250941242	23.288286742659984
20-21	25.006676850774234	24.62889871150829	26.441098049886353	23.923326387831125
22-23	24.8705567170049	24.300326158631965	26.32777316155054	24.501343962812594
24-25	24.40419478100828	24.910031149773815	26.25461081116566	24.431163258052248
26-27	24.361785805108553	25.023656181064407	26.378297819491507	24.236260194335525
28-29	24.29723370463101	25.303667369164362	26.340824835003723	24.0582740912009
30-31	24.706984094034766	24.948511345837275	26.25984937665093	24.08465518347703
32-33	24.31581720901032	24.911010770380436	26.307809730869124	24.46536228974012
34-35	24.440724467071842	24.984172353826033	26.452765010685326	24.122338168416803
36-37	24.519475158257748	25.10909560284913	26.278282279124465	24.093146959768657
38-39	24.505642972402114	25.232944301835374	26.3417565172805	23.91965620848201
40-41	24.45801583600623	25.053470251083226	26.328535771176597	24.15997814173394
42-43	24.405376018811513	24.946396239353543	26.20952587100382	24.438701870831125
44-45	24.338783613792025	25.053389959316586	26.24006349427895	24.36776293261244
46-47	24.496709985486795	25.172869178898015	25.95923764869529	24.3711831869199
48-49	24.39358097119303	25.325150362822384	25.874736088851563	24.40653257713302
50-51	24.450536452893747	25.387954027447744	25.832474827342715	24.32903469231579
52-53	24.56451556625839	25.39834517393652	25.71559606442124	24.32154319538385
54-55	24.35002126133223	25.143846662917102	25.839615628232448	24.66651644751822
56-57	24.438580502999223	25.319867032302206	25.670648876924453	24.570903587774115
58-59	24.347211043344608	25.36516735987207	25.825149015013572	24.462472581769752
60-61	24.598390486745288	25.255419155904445	25.91093979346472	24.23525056388554
62-63	24.373975599796207	25.254113346602175	26.028599080327762	24.343311973273853
64-65	24.26946391081936	24.99852154114551	25.97306247967119	24.75895206836394
66-67	24.31939728817529	25.440699607485044	25.760530986326145	24.479372118013522
68-69	24.276830393008865	25.359126853294452	25.781879503223497	24.582163250473183
70-71	24.159532854174532	25.220741364541045	26.00757739956161	24.61214838172281
72-73	24.394163005657624	25.2895122107724	25.78043289848717	24.535891885082805
74-75	24.211871618980254	25.412996718263287	25.779345935501958	24.5957857272545
76-77	24.245578354198276	25.145564722521453	25.84358937131357	24.7652675519667
78-79	24.065472168710148	25.13558226970382	25.9874419519234	24.81150360966263
80-81	24.200715574085574	25.456311540849818	25.644903752328574	24.698069132736038
82-83	24.19160529224633	25.48287814834069	25.677070303651828	24.648446255761154
84-85	24.175446917528724	25.410132732790984	25.624365728398097	24.79005462128219
86-87	24.0269670895059	25.55746769567403	25.70330287708093	24.71226233773914
88-89	24.047887427229135	25.309712322966615	25.67968755544306	24.962712694361187
90-91	24.060121695840827	25.24434677341616	25.809299814245	24.886231716498013
92-93	23.966793814128152	25.399834412608296	25.849640734498358	24.783731038765193
94-95	24.06659556027627	25.11757836906937	25.8726229653139	24.943203105340462
96-97	23.968573367496145	25.157658023948464	25.830131185787643	25.043637422767752
98-99	23.876163370911108	25.22860839031602	25.745777120680096	25.149451118092774
100-101	23.82521659422218	25.195866229042846	25.905319494958455	25.073597681776516
102-103	23.67500893740798	25.11682204992352	25.991993146952275	25.216175865716224
104-105	23.82453650314912	25.12540288003785	25.868298885242027	25.18176173157101
106-107	23.78469260154334	25.125100498805253	25.8191521227388	25.27105477691261
108-109	23.722260055337212	25.134432409144097	25.923477095556198	25.219830439962493
110-111	23.62845468090838	25.283590836740444	25.81879341683085	25.26916106552033
112-113	23.588639211704265	25.320635457577122	25.84478210035962	25.245943230358993
114-115	23.526420059729737	25.355717200390316	25.797391998580682	25.320470741299268
116-117	23.387882517244304	25.185246733953488	25.935341135198254	25.49152961360395
118-119	23.551016908055423	25.16325528857243	25.84874416734185	25.436983636030302
120-121	23.335654585804285	25.30866004592236	25.97103017776063	25.384655190512728
122-123	23.27194792683428	25.41032929541373	25.745707166210753	25.572015611541232
124-125	23.15708650311524	25.329632158778733	25.835139668723567	25.67814166938246
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	6.5
2	15.5
3	39.5
4	41.5
5	44.0
6	158.0
7	403.5
8	676.0
9	774.5
10	618.0
11	469.0
12	448.5
13	472.5
14	531.0
15	597.5
16	634.0
17	699.0
18	758.5
19	799.5
20	867.0
21	953.5
22	1079.5
23	1251.5
24	1485.5
25	1861.0
26	2471.5
27	3388.0
28	4407.5
29	5537.5
30	7182.0
31	9071.0
32	11738.5
33	14422.0
34	16809.5
35	19621.0
36	23017.5
37	26686.0
38	30085.5
39	33844.5
40	37258.5
41	40161.0
42	43606.5
43	48796.5
44	54339.0
45	59592.0
46	63175.5
47	66429.5
48	71464.0
49	74577.5
50	75814.5
51	75764.0
52	74809.5
53	74591.5
54	74943.5
55	73107.0
56	68212.0
57	62027.0
58	57360.5
59	53262.0
60	46152.5
61	38545.5
62	31673.0
63	24443.0
64	18198.5
65	13735.0
66	10514.0
67	8239.5
68	6410.5
69	4857.0
70	3705.5
71	2834.0
72	2180.0
73	1754.5
74	1417.5
75	1103.0
76	795.0
77	486.0
78	282.0
79	152.0
80	89.0
81	51.5
82	27.0
83	12.5
84	7.5
85	4.5
86	4.0
87	4.5
88	4.5
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02566604571394778
2	0.0021289807504657142
3	0.02844554836038913
4	0.16268961234808835
5	0.02134894585883675
6	0.06842307578580088
7	0.05109553801117715
8	0.044117212217983974
9	0.033235755048936985
10-11	0.0633076081492652
12-13	0.055708329637186194
14-15	0.05804429462728052
16-17	0.050356308583932106
18-19	0.03702060971643159
20-21	0.024098879328188296
22-23	0.0032526094798781754
24-25	0.005145036813625476
26-27	0.003548301250776191
28-29	0.00464236080309885
30-31	0.002572518406812738
32-33	0.009196014074928295
34-35	0.004701499157278453
36-37	0.006889618261923771
38-39	0.0024542416984535322
40-41	0.0033413170111475797
42-43	0.004169253969662025
44-45	0.005263313521984683
46-47	0.004494514917649841
48-49	0.002365534167184127
50-51	0.001655873917028889
52-53	0.0010053520210532541
54-55	0.005145036813625476
56-57	7.687986043348414E-4
58-59	7.687986043348414E-4
60-61	2.3655341671841273E-4
62-63	0.0012714746148614685
64-65	0.0
66-67	0.003489162896596588
68-69	5.026760105266271E-4
70-71	2.956917708980159E-5
72-73	0.0026907951151719446
74-75	0.004553653271829445
76-77	0.002099411573375913
78-79	1.4784588544900794E-4
80-81	0.0
82-83	0.005854697063780715
84-85	0.0020107040421065083
86-87	0.0
88-89	0.0015375972086696828
90-91	7.687986043348414E-4
92-93	0.0
94-95	0.0033413170111475797
96-97	0.0016263047399390877
98-99	7.392294272450398E-4
100-101	0.0
102-103	0.0013897513232206749
104-105	0.0
106-107	5.618143647062302E-4
108-109	0.0026316567609923416
110-111	8.870753126940477E-5
112-113	6.50521895975635E-4
114-115	0.0
116-117	0.001744581448298294
118-119	0.002365534167184127
120-121	0.003104763594429167
122-123	0.0012714746148614685
124-125	0.103876519116473
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1690950.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.658201186260094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63918760082402	44.76535898626102
2	12.114129173923736	13.969577942128753
3	4.024841245893322	6.961953188954248
4	1.9065967876606997	4.397237646560715
5	1.0653796561865676	3.0713937278076857
6	0.701354081500576	2.4263288840378925
7	0.47451034450845364	1.9151589036041008
8	0.3372388231356956	1.5555667129740416
9	0.26260759411623497	1.3627333345134247
>10	1.3818712756851859	14.048949475400828
>50	0.06685357999460256	2.583880043184402
>100	0.02440042635238036	2.3179918756198936
>500	7.205871529977766E-4	0.2809222050503995
>1k	3.0882306557047565E-4	0.3429470739026428
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2442	0.14441586090659098	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2024	0.11969602885951683	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.913835417960318E-5
2	0.0	0.0	0.0	0.0	5.913835417960318E-5
3	0.0	0.0	0.0	0.0	5.913835417960318E-5
4	0.0	0.0	0.0	0.0	5.913835417960318E-5
5	0.0	0.0	0.0	0.0	1.1827670835920637E-4
6	0.0	0.0	0.0	0.0	1.7741506253880955E-4
7	0.0	0.0	0.0	0.0	1.7741506253880955E-4
8	0.0	0.0	0.0	0.0	2.3655341671841273E-4
9	0.0	0.0	0.0	0.0	2.956917708980159E-4
10-11	0.0	0.0	0.0	2.956917708980159E-5	2.956917708980159E-4
12-13	0.0	0.0	0.0	5.913835417960318E-5	2.956917708980159E-4
14-15	0.0	0.0	0.0	5.913835417960318E-5	3.548301250776191E-4
16-17	0.0	0.0	0.0	5.913835417960318E-5	3.548301250776191E-4
18-19	0.0	0.0	0.0	5.913835417960318E-5	4.1396847925722225E-4
20-21	0.0	0.0	0.0	1.7741506253880952E-4	4.1396847925722225E-4
22-23	0.0	0.0	0.0	4.1396847925722225E-4	4.1396847925722225E-4
24-25	0.0	0.0	0.0	7.096602501552382E-4	4.1396847925722225E-4
26-27	0.0	0.0	0.0	0.0010053520210532541	4.1396847925722225E-4
28-29	0.0	0.0	0.0	0.003577870427865992	4.1396847925722225E-4
30-31	0.0	0.0	0.0	0.012685176971524883	4.1396847925722225E-4
32-33	0.0	0.0	0.0	0.02998314556905881	4.1396847925722225E-4
34-35	0.0	0.0	0.0	0.04964664833377687	4.7310683343682546E-4
36-37	0.0	0.0	0.0	0.07957065554865608	4.7310683343682546E-4
38-39	0.0	0.0	0.0	0.12697004642360804	5.618143647062302E-4
40-41	0.0	0.0	0.0	0.1764688488719359	5.913835417960318E-4
42-43	0.0	0.0	0.0	0.2317336408527751	5.913835417960318E-4
44-45	0.0	0.0	0.0	0.29007362725095365	5.913835417960318E-4
46-47	0.0	0.0	0.0	0.356633844880097	5.913835417960318E-4
48-49	0.0	0.0	0.0	0.4230462166237914	5.913835417960318E-4
50-51	0.0	0.0	0.0	0.491498861586682	5.913835417960318E-4
52-53	0.0	0.0	0.0	0.5628492859043732	6.209527188858334E-4
54-55	0.0	0.0	0.0	0.6448150447973033	6.50521895975635E-4
56-57	0.0	0.0	0.0	0.7349123273899287	6.50521895975635E-4
58-59	0.0	0.0	0.0	0.8340282089949437	6.50521895975635E-4
60-61	0.0	0.0	0.0	0.936367130902747	6.50521895975635E-4
62-63	0.0	0.0	0.0	1.0400070966025017	7.096602501552382E-4
64-65	0.0	5.913835417960318E-5	0.0	1.151394186699784	7.096602501552382E-4
66-67	0.0	5.913835417960318E-5	0.0	1.2809958898843845	7.096602501552382E-4
68-69	0.0	5.913835417960318E-5	0.0	1.4188769626541293	7.096602501552382E-4
70-71	0.0	5.913835417960318E-5	0.0	1.5662793104467903	7.096602501552382E-4
72-73	0.0	5.913835417960318E-5	0.0	1.715781069812827	9.166444897838493E-4
74-75	0.0	5.913835417960318E-5	0.0	1.8756616103373842	9.462136668736509E-4
76-77	0.0	1.1827670835920637E-4	0.0	2.057393772731305	9.462136668736509E-4
78-79	0.0	1.1827670835920637E-4	0.0	2.261953339838552	9.462136668736509E-4
80-81	0.0	1.1827670835920637E-4	0.0	2.472574588249209	9.757828439634525E-4
82-83	0.0	1.1827670835920637E-4	0.0	2.6798249504716285	0.0010644903752328573
84-85	0.0	1.1827670835920637E-4	0.0	2.8955025281646414	0.0010644903752328573
86-87	0.0	1.1827670835920637E-4	0.0	3.136077352967267	0.001094059552322659
88-89	0.0	1.1827670835920637E-4	0.0	3.4106271622460747	0.0011236287294124605
90-91	0.0	1.1827670835920637E-4	0.0	3.6999024217156036	0.0011236287294124605
92-93	0.0	1.1827670835920637E-4	0.0	4.001892427333747	0.0011236287294124605
94-95	0.0	1.1827670835920637E-4	0.0	4.3099441142553	0.0011236287294124605
96-97	0.0	1.1827670835920637E-4	0.0	4.634643247878412	0.0011827670835920638
98-99	0.0	1.1827670835920637E-4	0.0	5.001182767083592	0.0012419054377716668
100-101	0.0	1.1827670835920637E-4	0.0	5.404713326828114	0.0012419054377716668
102-103	0.0	1.7741506253880955E-4	0.0	5.818918359502055	0.00130104379195127
104-105	0.0	1.7741506253880955E-4	0.0	6.238179721458351	0.00130104379195127
106-107	0.0	1.7741506253880955E-4	0.0	6.6860344776604865	0.00130104379195127
108-109	0.0	1.7741506253880955E-4	0.0	7.169372246370383	0.00130104379195127
110-111	0.0	1.7741506253880955E-4	0.0	7.707117300925516	0.0013601821461308732
112-113	0.0	1.7741506253880955E-4	0.0	8.24294035896981	0.0013601821461308732
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2345	0.0	46.045784	1
GTATCAA	3925	0.0	45.90092	1
TCAACGC	4895	0.0	36.5473	4
CAACGCA	4940	0.0	36.055183	5
GTACATG	7945	0.0	34.68983	1
AACGCAG	5180	0.0	34.51398	6
TATCAAC	5305	0.0	33.730717	2
ATCAACG	5340	0.0	33.39695	3
TACATGG	8290	0.0	32.881485	2
ACATGGG	8275	0.0	32.14724	3
CATGGGG	7235	0.0	28.518345	4
ACGCAGA	6300	0.0	28.37648	7
ATGGGGG	3970	0.0	27.15886	5
CGCAGAG	6830	0.0	26.26097	8
GCAGAGT	7530	0.0	23.81971	9
AGTACAT	5775	0.0	23.239151	2
GAGTACA	6005	0.0	22.84909	1
GAGTACT	3490	0.0	21.750233	12-13
AGAGTAC	6855	0.0	21.427645	10-11
GTACTTT	3625	0.0	20.687744	14-15
>>END_MODULE