FastQCFastQC Report
Mon 15 Aug 2016
SRR2921594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716879
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44650.036.1258241
GTACATG108500.034.7251361
TACATGG111850.033.253552
CATGGGG89450.032.9320564
ACATGGG115300.032.1036953
TCAACGC50500.031.110474
CAACGCA51400.030.3306355
GGTATCA32350.030.174461
ATGGGGG54300.029.477855
AACGCAG53550.029.3334056
ATCAACG54250.028.9599763
TATCAAC55050.028.8634282
ACGCAGA61950.025.3530377
CGCAGAG66700.023.3677858
ATGGGGA31200.022.3138545
GAGTACA80000.021.5019471
GCAGAGT73800.020.9584459
AGTACAT79700.020.757762
TGGGGGG44000.020.4193696
ATACCGT2701.1059892E-619.8333826
Produced by FastQC (version 0.11.5)