FastQCFastQC Report
Mon 15 Aug 2016
SRR2921594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1716879
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29200.17007605078750454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22760.13256612725765765No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37200.043.7876361
GTATCAA57750.043.7038961
TCAACGC72500.034.878414
CAACGCA73800.034.301765
TATCAAC74750.034.074882
AACGCAG76250.033.448596
ATCAACG76000.033.3564833
GTACATG110350.031.090471
TACATGG113200.030.1416652
CATGGGG86800.030.0942294
ACATGGG113550.029.4175033
ACGCAGA88950.028.538927
ATGGGGG50350.028.3919755
CGCAGAG93900.027.0963528
CGCGATA600.001290598424.78436104-105
GCAGAGT104450.024.0171899
GAGTACT39250.023.35773312-13
GTAAGTA9500.023.2324872
ACCGTAT1350.002534322.0691078
AGTACTT44700.021.57531212-13
Produced by FastQC (version 0.11.5)