FastQCFastQC Report
Mon 15 Aug 2016
SRR2921593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615580
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG85500.037.032931
GTATCAA33550.035.657111
TACATGG88250.035.132722
ACATGGG89300.034.3864253
GGTATCA23900.031.8753281
CATGGGG77400.031.677074
ATGGGGG45550.031.6156625
CAACGCA36700.031.1322485
TCAACGC36900.030.9644684
AACGCAG39350.029.0320686
ATCAACG39450.028.9629633
TATCAAC40650.028.8399432
GTACCGT3007.2759576E-1225.7836046
GTATACG1200.001285918524.7988381
ACGCAGA47450.023.9462747
GTATAGG3603.6379788E-1223.1455821
CGCAGAG50650.022.785038
TGGGGGG37000.021.8704996
TACCGTG3557.8216544E-1121.7849127
GAGTACA60900.021.402741
Produced by FastQC (version 0.11.5)