##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921593_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1615580
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.025081394916995	31.0	31.0	34.0	26.0	34.0
2	30.09813874893227	31.0	31.0	34.0	26.0	34.0
3	30.264738979190135	31.0	31.0	34.0	26.0	34.0
4	33.78992188563859	37.0	35.0	37.0	30.0	37.0
5	33.76057638742743	37.0	35.0	37.0	30.0	37.0
6	33.74440758117828	37.0	35.0	37.0	29.0	37.0
7	33.568953564664085	37.0	35.0	37.0	28.0	37.0
8	33.60721846024338	37.0	35.0	37.0	28.0	37.0
9	35.21336547865163	39.0	37.0	39.0	28.0	39.0
10-11	35.28711577266368	39.0	37.0	39.0	29.0	39.0
12-13	35.25111105609131	39.0	37.0	39.0	29.0	39.0
14-15	36.544154111835994	40.0	38.0	41.0	28.5	41.0
16-17	36.4978209064237	40.0	38.0	41.0	27.5	41.0
18-19	36.441791802324865	40.0	38.0	41.0	27.0	41.0
20-21	36.49773858304758	40.0	38.0	41.0	28.0	41.0
22-23	36.41613878607063	40.0	38.0	41.0	27.0	41.0
24-25	36.401717339902696	40.0	38.0	41.0	27.0	41.0
26-27	36.36571850357147	40.0	38.0	41.0	27.5	41.0
28-29	36.30799589002092	40.0	38.0	41.0	27.5	41.0
30-31	36.21804862649946	40.0	37.0	41.0	27.0	41.0
32-33	36.1330853315837	40.0	37.0	41.0	27.0	41.0
34-35	36.012044281310736	40.0	37.0	41.0	27.0	41.0
36-37	35.91105856720188	40.0	37.0	41.0	26.0	41.0
38-39	35.79728394756063	40.0	37.0	41.0	26.0	41.0
40-41	35.68824570742396	40.0	36.0	41.0	25.5	41.0
42-43	35.43718169326186	40.0	35.5	41.0	24.5	41.0
44-45	35.08036866017158	40.0	35.0	41.0	23.5	41.0
46-47	34.829993253196996	39.0	35.0	41.0	23.0	41.0
48-49	34.64092554995729	39.0	35.0	41.0	22.5	41.0
50-51	34.43420845764369	39.0	34.5	40.5	21.5	41.0
52-53	34.229354473316086	38.5	34.0	40.0	20.5	41.0
54-55	33.985499634806075	38.0	34.0	40.0	19.5	41.0
56-57	33.731146399435495	38.0	34.0	40.0	18.0	41.0
58-59	33.73662647470259	38.0	34.0	40.0	18.5	41.0
60-61	33.521191460651906	37.0	34.0	40.0	18.0	41.0
62-63	33.20289184070117	37.0	33.0	40.0	14.5	41.0
64-65	32.90243318189133	36.0	33.0	39.5	13.0	41.0
66-67	32.579421631859766	36.0	33.0	39.0	11.0	41.0
68-69	32.24222198838807	35.0	33.0	39.0	8.0	40.5
70-71	31.87747496255215	35.0	32.5	37.5	7.0	40.0
72-73	31.53084124586836	35.0	32.0	37.0	6.0	39.0
74-75	31.168637579073767	35.0	32.0	37.0	2.0	39.0
76-77	30.87775133388628	35.0	32.0	36.0	2.0	39.0
78-79	30.614256799415692	35.0	31.5	36.0	2.0	37.0
80-81	30.36684812884537	35.0	31.0	35.0	2.0	37.0
82-83	30.159359486995385	35.0	31.0	35.0	2.0	36.5
84-85	29.95182504116169	35.0	31.0	35.0	2.0	36.0
86-87	29.78542350115748	35.0	31.0	35.0	2.0	36.0
88-89	29.63520593223486	34.5	31.0	35.0	2.0	35.5
90-91	29.461871897399078	34.0	31.0	35.0	2.0	35.0
92-93	29.38298382005224	34.0	31.0	35.0	2.0	35.0
94-95	29.265666200373857	34.0	30.0	35.0	2.0	35.0
96-97	29.182959061142128	34.0	30.0	35.0	2.0	35.0
98-99	29.08903180282004	34.0	30.0	35.0	2.0	35.0
100-101	28.988389928075364	34.0	30.0	35.0	2.0	35.0
102-103	28.113893462409784	33.5	28.0	34.5	2.0	35.0
104-105	29.042595847930777	34.0	30.5	35.0	2.0	35.0
106-107	29.325489297961106	34.5	31.0	35.0	2.0	35.0
108-109	29.331640030205868	35.0	31.0	35.0	2.0	35.0
110-111	29.27983696257691	35.0	31.0	35.0	2.0	35.0
112-113	29.204796729347976	35.0	31.0	35.0	2.0	35.0
114-115	29.09380067839414	35.0	31.0	35.0	2.0	35.0
116-117	28.976754787754246	35.0	31.0	35.0	2.0	35.0
118-119	28.695856596392627	34.0	30.0	35.0	2.0	35.0
120-121	28.570088451206377	34.0	30.0	35.0	2.0	35.0
122-123	28.32102681389965	34.0	30.0	35.0	2.0	35.0
124-125	27.553600255016775	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	70868.0
3	12222.0
4	5874.0
5	4988.0
6	5254.0
7	5603.0
8	5868.0
9	5954.0
10	6552.0
11	6922.0
12	6892.0
13	6714.0
14	6655.0
15	5898.0
16	5130.0
17	4704.0
18	4428.0
19	4269.0
20	4317.0
21	4510.0
22	5197.0
23	6842.0
24	9621.0
25	12006.0
26	13872.0
27	15695.0
28	18581.0
29	22278.0
30	26987.0
31	34030.0
32	44361.0
33	61990.0
34	97187.0
35	159848.0
36	316925.0
37	469272.0
38	117195.0
39	71.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.28527489046296	16.671485450471675	11.443672544757423	25.59956711430795
2	16.962032839006657	18.95572355013879	37.89609482752217	26.186148783332385
3	20.43902031366553	22.724996363163648	28.047901003766835	28.78808231940399
4	13.871167034172469	14.28567891177013	33.79992744804054	38.04322660601686
5	14.747810174016026	37.7922270076784	32.33575453890923	15.12420827939635
6	35.88458974632196	32.813107896804055	16.416590053600235	14.88571230327375
7	31.787413824282044	30.87908521827879	18.768718369207367	18.564782588231797
8	27.99444164751648	35.22252916184429	18.32027033805261	18.46275885258662
9	28.35992030718139	15.90779357628334	17.5266622998238	38.20562381671147
10-11	27.247661284154944	25.041885252830255	25.902564885373273	21.807888577641528
12-13	26.844039287009856	22.426169714633087	26.214533420329655	24.515257578027406
14-15	24.374292460058143	23.782040705390042	23.24628379978473	28.597383034767088
16-17	25.115443197860166	26.84483121579117	23.98320826212943	24.056517324219236
18-19	25.004899552213438	26.183207028605025	25.290915750303338	23.5209776688782
20-21	25.552125180053437	25.132039187800725	25.122999754511284	24.192835877634554
22-23	25.620734823222808	24.731253035912697	24.97347591377683	24.674536227087664
24-25	25.11050909875531	25.160504811197303	24.931143675913862	24.797842414133527
26-27	24.779269320068696	25.388773007711524	25.18498588630214	24.646971785917636
28-29	24.932620939415038	25.712128168983124	25.026711675827535	24.3285392157743
30-31	25.455694764451554	25.165297020768655	25.08468773342719	24.294320481352603
32-33	24.94988860178132	25.190887665955795	25.152470217190153	24.706753515072734
34-35	24.914415821609662	25.584692344345466	25.253068166091357	24.247823667953515
36-37	24.968723865624977	25.738726508852956	25.061267796135933	24.231281829386134
38-39	25.120203655156892	25.651226304797405	25.030838316217103	24.1977317238286
40-41	24.95516944587206	25.420317405276876	25.187062298370865	24.437450850480193
42-43	24.896370883739447	25.293583584764146	25.058914828025657	24.75113070347075
44-45	24.791745855655858	25.39233372169471	25.23920749516818	24.57671292748125
46-47	24.69477031237715	25.394193748494825	25.24929438560627	24.661741553521757
48-49	24.597302700457103	25.54258960815914	25.34301321655726	24.517094474826504
50-51	24.67194802308228	25.456257621061695	25.338035393611296	24.533758962244733
52-53	24.75139671030148	25.461638757225003	25.276494158085818	24.5104703743877
54-55	24.528484113066614	25.155232109110553	25.346441788152553	24.969841989670282
56-57	24.688226498967616	25.437145360157754	25.01815570256223	24.8564724383124
58-59	24.586842795196414	25.633065081526407	25.314400630704892	24.465691492572287
60-61	25.03648792253633	25.317464272790673	25.29675553881247	24.349292265860523
62-63	24.770631410193904	25.38236352959713	25.43164384201945	24.415361218189513
64-65	24.665720692443227	25.156723412738213	25.379287374339583	24.79826852047898
66-67	24.63403278412454	25.561395318888643	25.140108917192723	24.664462979794102
68-69	24.647029088622933	25.37858608571348	25.235419703470697	24.738965122192894
70-71	24.52037977642544	25.300460245928917	25.4825461058674	24.69661387177824
72-73	24.652543361851723	25.58675047909843	25.10144566848896	24.659260490560886
74-75	24.6009399843078	25.572122981022343	25.14914551805144	24.67779151661841
76-77	24.541051616971053	25.25263093056275	25.30460107574177	24.90171637672443
78-79	24.551259846590227	25.29249174955803	25.322301994809983	24.83394640904176
80-81	24.57518387263425	25.57248068722136	25.113839919297547	24.738495520846843
82-83	24.570712886537944	25.650709865307608	25.207214301811003	24.571362946343445
84-85	24.666728157269024	25.629757654806202	25.04375015088927	24.6597640370355
86-87	24.471219606715472	25.742155930205602	25.080129966084634	24.706494496994296
88-89	24.519983210091777	25.489334198418085	25.146571193668372	24.84411139782177
90-91	24.767674879171125	25.323917957086632	25.15212335038485	24.756283813357385
92-93	24.580486514007514	25.61445645159543	25.327233982627373	24.477823051769683
94-95	24.67219132562101	25.30190216314726	25.269991036409017	24.75591547482271
96-97	24.490768082190254	25.485460333025046	25.213387116543906	24.81038446824079
98-99	24.464064198399	25.485734376895707	25.146271228826883	24.903930195878406
100-101	24.386965341481336	25.56020098461912	25.207047956386063	24.84578571751348
102-103	24.1567045508707	25.557539072147527	25.428975009547912	24.856781367433864
104-105	24.310795946575816	25.507219643421976	25.348168983047596	24.833815426954615
106-107	24.24146576448571	25.523647587394265	25.401059009332023	24.833827638788
108-109	24.290611385813	25.523009185102648	25.422506983618483	24.76387244546587
110-111	24.131582245867165	25.843612229139296	25.253540938400903	24.771264586592636
112-113	24.079209906437754	25.94537915905705	25.33165836766363	24.64375256684156
114-115	24.164502858579034	25.954492399872354	25.259742088726604	24.62126265282201
116-117	24.05392222403795	25.746481610783455	25.36728294187849	24.832313223300105
118-119	24.127302255183615	25.700968369332827	25.355026858803292	24.816702516680262
120-121	24.089063688633622	25.93919271486163	25.35007919839709	24.621664398107658
122-123	24.0139121736781	25.948283679347796	25.33109964417988	24.706704502794228
124-125	24.023676464698738	25.78031839542465	25.395262285518967	24.80074285435764
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	382.0
1	317.0
2	229.5
3	176.0
4	138.0
5	132.0
6	147.0
7	155.0
8	165.5
9	197.5
10	211.5
11	208.0
12	213.0
13	251.0
14	299.5
15	313.5
16	344.5
17	386.0
18	421.5
19	471.0
20	548.5
21	632.5
22	750.0
23	958.5
24	1130.5
25	1418.5
26	1929.5
27	2570.0
28	3507.0
29	4700.5
30	6144.5
31	7858.0
32	10137.0
33	12572.0
34	14981.0
35	17710.5
36	20983.5
37	23949.5
38	26899.5
39	30618.0
40	34028.0
41	36898.5
42	40078.5
43	45057.0
44	50433.0
45	55592.0
46	58905.5
47	62306.5
48	68514.0
49	73098.0
50	75255.5
51	75010.5
52	73596.5
53	72682.0
54	72920.0
55	71819.5
56	67188.0
57	61699.5
58	57297.5
59	52580.5
60	45136.5
61	37414.0
62	30738.5
63	23948.0
64	17770.0
65	13431.5
66	10450.0
67	8093.0
68	6329.0
69	5032.0
70	4018.5
71	3076.5
72	2341.0
73	1937.0
74	1527.0
75	1178.5
76	947.5
77	632.0
78	393.5
79	275.0
80	203.0
81	151.5
82	122.0
83	95.0
84	75.0
85	62.0
86	45.0
87	37.0
88	33.5
89	31.0
90	21.0
91	15.5
92	17.0
93	13.5
94	15.5
95	13.5
96	7.5
97	8.0
98	4.5
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023087683680164398
2	0.011389098651877344
3	0.010213050421520445
4	0.011512893202441228
5	0.016650367050842423
6	0.08715136359697447
7	0.052984067641342426
8	0.04413275727602471
9	0.023397170056574112
10-11	0.04651580237437948
12-13	0.011512893202441228
14-15	0.024047091447034503
16-17	0.030948637640971045
18-19	0.039397615716956144
20-21	0.02689436611000384
22-23	0.03333168273932581
24-25	0.02732764703697743
26-27	0.04082125304844081
28-29	0.04020228029562139
30-31	0.023613810520060907
32-33	0.026522982458312184
34-35	0.04020228029562139
36-37	0.008108543061934414
38-39	0.018600131222223596
40-41	0.03775733792198467
42-43	0.020085665828990205
44-45	0.015226729719357754
46-47	0.01986902536550341
48-49	0.025315985590314314
50-51	0.02485175602569975
52-53	0.022035430000371385
54-55	0.020890330407655453
56-57	0.023830450983547705
58-59	0.015133883806434842
60-61	0.0197761794525805
62-63	0.02045704948068186
64-65	0.019435744438529814
66-67	0.02525408831503237
68-69	0.02020946037955409
70-71	0.04224489037992547
72-73	0.01897151487391525
74-75	0.007891902598447615
76-77	0.01420542467720571
78-79	0.02249965956498595
80-81	0.016309932036791742
82-83	0.02129266269698808
84-85	0.009717872219264907
86-87	0.014576808328897363
88-89	0.020054717191349238
90-91	0.01726933980366184
92-93	0.006653957092808775
94-95	0.009655974943982966
96-97	0.01275083870808007
98-99	0.005446960224810904
100-101	0.008232337612498298
102-103	0.00266158283712351
104-105	0.0035281446910706993
106-107	8.356132163062183E-4
108-109	0.011915225491773852
110-111	7.737159410242762E-4
112-113	0.001516483244407581
114-115	0.011110560913108605
116-117	0.004209014719172063
118-119	0.2766498718726402
120-121	0.0010522536797930157
122-123	0.0010213050421520444
124-125	0.3116837296822194
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1615580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.7157590698403
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.95147303102257	53.68267543164013
2	9.532718018107564	12.338341650811493
3	3.0128466695002887	5.849359775332545
4	1.3903591610622963	3.599125939514114
5	0.782705538255698	2.532669151819271
6	0.4940801770975507	1.9184864221337472
7	0.3423073382585187	1.5506875461403637
8	0.25229391717773886	1.3061913887088634
9	0.18900352330708195	1.1008355829922858
>10	0.9813192925840816	11.101276364354225
>50	0.04819261755555803	2.114492717169597
>100	0.021455683199841168	2.3481671990309185
>500	0.0012450328712230207	0.5576908303524988
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.189727528194209E-5
7	0.0	0.0	0.0	0.0	6.189727528194209E-5
8	0.0	0.0	0.0	0.0	6.189727528194209E-5
9	0.0	0.0	0.0	0.0	1.2379455056388418E-4
10-11	0.0	0.0	0.0	0.0	1.2379455056388418E-4
12-13	0.0	0.0	0.0	0.0	1.2379455056388418E-4
14-15	0.0	0.0	0.0	0.0	1.8569182584582628E-4
16-17	0.0	0.0	0.0	0.0	1.8569182584582628E-4
18-19	0.0	0.0	0.0	6.189727528194209E-5	2.785377387687394E-4
20-21	0.0	0.0	0.0	6.189727528194209E-5	3.0948637640971046E-4
22-23	0.0	0.0	0.0	2.4758910112776837E-4	3.404350140506815E-4
24-25	0.0	0.0	0.0	4.951782022555367E-4	3.7138365169165255E-4
26-27	0.0	0.0	0.0	8.975104915881604E-4	4.3328092697359464E-4
28-29	0.0	0.0	0.0	0.002847274662969336	4.3328092697359464E-4
30-31	0.0	0.0	0.0	0.011915225491773852	4.3328092697359464E-4
32-33	0.0	0.0	0.0	0.029463103034204435	5.570754775374788E-4
34-35	0.0	0.0	0.0	0.052303197613241065	6.189727528194209E-4
36-37	0.0	0.0	0.0	0.08353037299298086	6.189727528194209E-4
38-39	0.0	0.0	0.0	0.1361740056202726	6.189727528194209E-4
40-41	0.0	0.0	0.0	0.20206365515789992	6.189727528194209E-4
42-43	0.0	0.0	0.0	0.2630634199482539	6.49921390460392E-4
44-45	0.0	0.0	0.0	0.33492615655058866	7.427673033833051E-4
46-47	0.0	0.0	0.0	0.4095742705406108	8.046645786652472E-4
48-49	0.0	0.0	0.0	0.4828915931120712	9.284591292291314E-4
50-51	0.0	0.0	0.0	0.5607274167791134	9.284591292291314E-4
52-53	0.0	0.0	0.0	0.6428651010782505	0.0010832023174339866
54-55	0.0	0.0	0.0	0.7410341796754107	0.0012379455056388418
56-57	0.0	0.0	0.0	0.8451453966996373	0.0012379455056388418
58-59	0.0	0.0	0.0	0.9599957909852809	0.0012379455056388418
60-61	0.0	0.0	0.0	1.070018197798933	0.0012379455056388418
62-63	0.0	0.0	0.0	1.1897275281942088	0.0012379455056388418
64-65	0.0	0.0	0.0	1.3164312506963443	0.0012379455056388418
66-67	0.0	0.0	0.0	1.4634991767662386	0.0012379455056388418
68-69	0.0	0.0	0.0	1.623813119746469	0.0012379455056388418
70-71	0.0	0.0	0.0	1.786912440114386	0.0012998427809207838
72-73	0.0	0.0	0.0	1.9400153505242699	0.0014855346067666102
74-75	0.0	0.0	0.0	2.103857438195571	0.0014855346067666102
76-77	0.0	0.0	0.0	2.2996075712747124	0.0015474318820485524
78-79	0.0	0.0	0.0	2.5160932915733047	0.0016093291573304944
80-81	0.0	0.0	0.0	2.72852474034093	0.0017950209831763208
82-83	0.0	0.0	0.0	2.940306267718095	0.002042610084304089
84-85	0.0	0.0	0.0	3.163786380123547	0.002042610084304089
86-87	0.0	0.0	0.0	3.425642803203803	0.002042610084304089
88-89	0.0	0.0	0.0	3.729032297998242	0.002042610084304089
90-91	0.0	0.0	0.0	4.030286336795454	0.002042610084304089
92-93	0.0	0.0	0.0	4.323772267544783	0.002042610084304089
94-95	0.0	0.0	0.0	4.626728481412249	0.0022283019101499153
96-97	0.0	0.0	0.0	4.977128956783322	0.0022592505477908864
98-99	0.0	0.0	0.0	5.367453174711249	0.002290199185431857
100-101	0.0	0.0	0.0	5.7837432996199505	0.002290199185431857
102-103	0.0	0.0	0.0	6.199600143601678	0.002290199185431857
104-105	0.0	0.0	0.0	6.616261652162072	0.002290199185431857
106-107	0.0	0.0	0.0	7.066069151635945	0.002290199185431857
108-109	0.0	0.0	0.0	7.562206761658352	0.0023520964607137993
110-111	0.0	0.0	0.0	8.091180876217829	0.0024758910112776837
112-113	0.0	3.0948637640971046E-5	0.0	8.613222495945728	0.0025377882865596254
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8550	0.0	37.03293	1
GTATCAA	3355	0.0	35.65711	1
TACATGG	8825	0.0	35.13272	2
ACATGGG	8930	0.0	34.386425	3
GGTATCA	2390	0.0	31.875328	1
CATGGGG	7740	0.0	31.67707	4
ATGGGGG	4555	0.0	31.615662	5
CAACGCA	3670	0.0	31.132248	5
TCAACGC	3690	0.0	30.964468	4
AACGCAG	3935	0.0	29.032068	6
ATCAACG	3945	0.0	28.962963	3
TATCAAC	4065	0.0	28.839943	2
GTACCGT	300	7.2759576E-12	25.783604	6
GTATACG	120	0.0012859185	24.798838	1
ACGCAGA	4745	0.0	23.946274	7
GTATAGG	360	3.6379788E-12	23.145582	1
CGCAGAG	5065	0.0	22.78503	8
TGGGGGG	3700	0.0	21.870499	6
TACCGTG	355	7.8216544E-11	21.784912	7
GAGTACA	6090	0.0	21.40274	1
>>END_MODULE