FastQCFastQC Report
Mon 15 Aug 2016
SRR2921593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1615580
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.13023186719320617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17160.10621572438381263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43400.043.428841
GGTATCA28350.043.1302221
GTACATG85050.035.275611
TCAACGC54100.034.3834574
CAACGCA54850.033.988275
ATGGGGG43350.033.799275
TACATGG87900.033.6464272
ACATGGG88800.033.036823
CATGGGG73350.032.431354
AACGCAG57600.032.3836336
TATCAAC57300.032.363232
ATCAACG57250.032.2873463
ACGCAGA68850.027.0905087
TACGGTC900.00943690926.4977954
CGCAGAG72250.025.8148578
TATCACG1401.0409348E-425.5546152
TGGGGGG31200.025.0219046
GCAGAGT79600.023.131769
GAGTACT32550.022.3145712-13
GTCCTAA4151.8189894E-1221.5587731
Produced by FastQC (version 0.11.5)