##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921593_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1615580
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.768317260674184	33.0	31.0	34.0	30.0	34.0
2	31.94126443753946	33.0	31.0	34.0	30.0	34.0
3	32.01810742891098	34.0	31.0	34.0	30.0	34.0
4	35.419035888040206	37.0	35.0	37.0	35.0	37.0
5	35.27507768108048	37.0	35.0	37.0	33.0	37.0
6	35.30137597642952	37.0	35.0	37.0	33.0	37.0
7	35.18999987620545	37.0	35.0	37.0	33.0	37.0
8	35.230931925376645	37.0	36.0	37.0	33.0	37.0
9	36.95970734968247	39.0	38.0	39.0	34.0	39.0
10-11	36.913461419428316	39.0	38.0	39.0	34.0	39.0
12-13	36.83816090815682	39.0	38.0	39.0	34.0	39.0
14-15	38.272043786132535	41.0	39.0	41.0	34.0	41.0
16-17	38.27516062342936	41.0	39.0	41.0	34.0	41.0
18-19	38.28117332475024	41.0	39.0	41.0	34.0	41.0
20-21	38.22135579791778	41.0	39.0	41.0	34.0	41.0
22-23	38.22567096646406	41.0	39.0	41.0	34.0	41.0
24-25	38.18492089528219	41.0	39.0	41.0	34.0	41.0
26-27	38.110888040208465	41.0	39.0	41.0	34.0	41.0
28-29	38.041622822763344	41.0	39.0	41.0	33.5	41.0
30-31	37.93468816152713	41.0	38.0	41.0	33.0	41.0
32-33	37.82479883385534	41.0	38.0	41.0	33.0	41.0
34-35	37.69629699550626	41.0	38.0	41.0	33.0	41.0
36-37	37.62613519602867	40.5	38.0	41.0	33.0	41.0
38-39	37.551315317099736	40.0	38.0	41.0	33.0	41.0
40-41	37.43437093799131	40.0	38.0	41.0	32.0	41.0
42-43	37.28152892459674	40.0	38.0	41.0	32.0	41.0
44-45	37.13637888560146	40.0	37.5	41.0	31.5	41.0
46-47	36.94366945617054	40.0	37.0	41.0	31.0	41.0
48-49	36.76346234788745	40.0	37.0	41.0	31.0	41.0
50-51	36.52303878483269	40.0	36.0	41.0	30.0	41.0
52-53	36.398351366072866	40.0	36.0	41.0	30.0	41.0
54-55	36.270030886740365	40.0	35.5	41.0	30.0	41.0
56-57	36.0743191299719	39.0	35.0	41.0	30.0	41.0
58-59	35.87553386399931	39.0	35.0	41.0	29.5	41.0
60-61	35.621941965114694	39.0	35.0	41.0	29.0	41.0
62-63	35.26164628183068	38.0	35.0	41.0	28.5	41.0
64-65	34.973779695217814	37.0	35.0	40.0	29.0	41.0
66-67	34.66970190272224	37.0	35.0	39.5	29.0	41.0
68-69	34.29881373871922	36.0	35.0	39.0	28.0	41.0
70-71	33.92660499634806	35.5	35.0	39.0	28.0	41.0
72-73	33.602067059508045	35.0	34.0	37.5	28.0	40.0
74-75	33.284248071899874	35.0	34.0	37.0	27.5	39.0
76-77	32.958424838138626	35.0	34.0	36.5	27.0	39.0
78-79	32.70334771413363	35.0	34.0	36.0	27.0	38.0
80-81	32.451397330989494	35.0	34.0	36.0	26.0	37.0
82-83	32.23463926267966	35.0	34.0	35.0	26.0	37.0
84-85	32.0281774966266	35.0	34.0	35.0	26.0	36.0
86-87	31.902081605367734	35.0	34.0	35.0	26.0	36.0
88-89	31.80941395659763	35.0	34.0	35.0	25.5	36.0
90-91	31.706618366159525	35.0	34.0	35.0	25.0	36.0
92-93	31.627147835452284	35.0	34.0	35.0	25.0	35.0
94-95	31.56201271370034	35.0	33.0	35.0	25.0	35.0
96-97	31.499792025155052	35.0	33.0	35.0	25.0	35.0
98-99	31.422870424243925	35.0	33.0	35.0	24.0	35.0
100-101	31.358185914656037	35.0	33.0	35.0	24.0	35.0
102-103	30.670036148008762	34.0	31.5	35.0	23.0	35.0
104-105	31.189194902140407	35.0	33.0	35.0	24.0	35.0
106-107	31.287654588445015	35.0	33.0	35.0	24.0	35.0
108-109	31.272984315230445	35.0	33.0	35.0	24.0	35.0
110-111	31.21143954493123	35.0	33.0	35.0	23.0	35.0
112-113	31.17728865175355	35.0	33.0	35.0	23.0	35.0
114-115	31.050266777256464	35.0	33.0	35.0	20.0	35.0
116-117	31.23384790601518	35.0	33.5	35.0	23.5	35.0
118-119	31.214601567239008	35.0	34.0	35.0	23.0	35.0
120-121	31.133875759789056	35.0	34.0	35.0	21.5	35.0
122-123	31.001337600118845	35.0	33.0	35.0	19.0	35.0
124-125	30.3599128486364	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24966.0
3	6765.0
4	3880.0
5	2907.0
6	2966.0
7	3378.0
8	3782.0
9	4009.0
10	4179.0
11	4535.0
12	5084.0
13	6221.0
14	8043.0
15	7485.0
16	4506.0
17	3933.0
18	3625.0
19	3323.0
20	3143.0
21	3308.0
22	3505.0
23	4612.0
24	5940.0
25	7581.0
26	9344.0
27	11178.0
28	13608.0
29	16277.0
30	20102.0
31	24792.0
32	32681.0
33	44631.0
34	69926.0
35	122962.0
36	288907.0
37	610959.0
38	218179.0
39	358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43358238340173	17.15647063776464	10.692147864913657	27.717799113919977
2	16.202222153446193	19.294605552288694	36.941598836309616	27.561573457955497
3	19.173682770166938	22.759356421359993	28.08907716826389	29.977883640209175
4	13.413057670977146	14.42019330506525	33.53779021644984	38.628958807507765
5	14.748702963063856	37.11655378214732	32.607446663612386	15.527296591176434
6	35.69863795962127	33.09174061340437	16.125168234376734	15.084453192597625
7	31.19247022570993	31.28238759090819	18.643548243267343	18.881593940114538
8	28.768729867598303	34.42307859020864	17.998258752370067	18.809932789822987
9	28.287189304855215	15.132550053000207	17.798472900547836	38.78178774159674
10-11	27.402523363704574	24.598631079990128	25.903449736642532	22.095395819662762
12-13	27.15325483638728	21.877269173304846	26.465640168141384	24.503835822166483
14-15	24.359366581409894	23.248964223120893	23.723625621620954	28.668043573848262
16-17	24.750921600913518	26.44324282148282	24.427238887997856	24.378596689605804
18-19	24.71009492249333	25.604151438852373	26.188373209696636	23.49738042895766
20-21	25.243099527096234	24.64535313315381	26.096134088353168	24.01541325139678
22-23	25.038060064801922	24.27948947053877	25.996099752497386	24.686350712161918
24-25	24.63639965088424	24.66360468211277	25.932306206708716	24.767689460294275
26-27	24.44641352222037	24.90195963129208	26.152320580167483	24.499306266320072
28-29	24.51834065919781	25.26668065606567	25.974569005805602	24.240409678930913
30-31	25.05892737325044	24.770418458628278	25.976294111966965	24.19436005615432
32-33	24.58375401048995	24.749651954779193	26.028768436801858	24.637825597929
34-35	24.657245097996736	24.94863860581747	26.20260561196351	24.191510684222287
36-37	24.618280623205454	25.120575133249336	26.052925852367075	24.20821839117814
38-39	24.791742492330776	25.114790770685858	26.049736683775414	24.043730053207952
40-41	24.732480013568274	24.842196091879245	26.04932054952745	24.376003345025033
42-43	24.58914310261797	24.807245442041786	25.882777497855187	24.720833957485056
44-45	24.463559827200186	24.94569829608093	25.97246320204567	24.618278674673213
46-47	24.55719105508754	24.9626975497838	25.752540760335513	24.72757063479315
48-49	24.40256395734083	25.20055869535412	25.809267992098047	24.587609355207004
50-51	24.503360448835636	25.13964215459922	25.781370894762055	24.57562650180309
52-53	24.661473654629933	25.27289929210677	25.533488675967625	24.53213837729567
54-55	24.365670290612005	24.84675839652567	25.79181921189325	24.995752100969078
56-57	24.48615658869625	25.132182449537932	25.48636085096901	24.895300110796807
58-59	24.385441551451947	25.313751396178624	25.725432594863552	24.575374457505877
60-61	24.754592625925444	25.0058647704631	25.76327124826455	24.476271355346906
62-63	24.62752183524706	25.050686617587846	25.852325920754016	24.469465626411075
64-65	24.45468500476609	24.79539855655554	25.78881887619307	24.9610975624853
66-67	24.371495131135752	25.263931964961138	25.573801137156728	24.790771766746385
68-69	24.38813409875954	25.12846081306882	25.652826664017446	24.830578424154194
70-71	24.294928137263398	24.93968110523775	25.8929300932173	24.872460664281558
72-73	24.37818952062292	25.217937357961972	25.539934059383697	24.86393906203141
74-75	24.289384091612504	25.23952336737852	25.545961002785518	24.92513153822346
76-77	24.23617533056772	24.909968964206705	25.744821900103616	25.10903380512196
78-79	24.126798041814162	24.885938584171473	25.808085903674044	25.17917747034032
80-81	24.22767674766957	25.186713130887977	25.502760618477577	25.082849502964876
82-83	24.240142370783037	25.2793562364593	25.470999690498296	25.009501702259364
84-85	24.336675527395997	25.184712007727374	25.403398397640935	25.075214067235695
86-87	23.999956671907302	25.33755679075007	25.43504499931913	25.2274415380235
88-89	24.080682834223786	25.10704489246921	25.515045707047186	25.297226566259823
90-91	24.188947495636988	24.91766840258596	25.67628586929509	25.21709823248196
92-93	24.114157144802487	25.20200175788262	25.629247700516224	25.054593396798673
94-95	24.016492405131068	24.954264277731507	25.651217571430827	25.3780257457066
96-97	23.846360490256945	25.058261495542418	25.657434166831212	25.437943847369425
98-99	23.893896239066162	25.033711022645576	25.50868285146507	25.563709886823194
100-101	23.768058530063506	25.002104507359586	25.666850295250004	25.562986667326904
102-103	23.575427639418034	25.032109708428372	25.81317256298919	25.579290089164402
104-105	23.829213038042067	24.95196771438121	25.72676685772292	25.492052389853797
106-107	23.718294114497564	24.90988483022881	25.738755604440904	25.633065450832724
108-109	23.763496236249978	24.902463134685984	25.75285832689071	25.58118230217333
110-111	23.55673215507634	25.225329502713734	25.594670887239463	25.623267454970467
112-113	23.48845565462936	25.206977456007134	25.686621075041728	25.61794581432178
114-115	23.483021577390165	25.183865856225008	25.64069869644338	25.692413869941443
116-117	23.3206586698474	24.996084946448878	25.767335021500298	25.915921362203427
118-119	23.548565703846094	24.884830136637802	25.67595463707837	25.890649522437737
120-121	23.257162770861278	25.02771517405005	25.802347325137525	25.912774729951142
122-123	23.141018419596403	25.154280677559882	25.671592919367995	26.033107983475716
124-125	22.931588260660522	24.966015940599455	25.774570134176628	26.32782566456339
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	18.5
2	19.0
3	43.0
4	47.5
5	57.5
6	137.5
7	319.5
8	564.0
9	627.0
10	528.5
11	449.5
12	447.0
13	494.0
14	511.0
15	531.0
16	550.5
17	611.5
18	725.5
19	779.0
20	806.5
21	854.5
22	957.0
23	1136.0
24	1290.0
25	1545.0
26	2021.0
27	2668.0
28	3533.5
29	4701.5
30	6074.5
31	7619.0
32	9906.5
33	12228.5
34	14422.5
35	17037.5
36	20358.0
37	23581.5
38	26398.5
39	30016.0
40	33777.0
41	36615.0
42	39767.5
43	45049.5
44	50432.5
45	55645.5
46	59109.0
47	62401.5
48	68495.5
49	72967.0
50	75417.0
51	75878.5
52	74736.5
53	73704.0
54	73865.0
55	72567.0
56	68217.5
57	62842.0
58	57757.0
59	52848.0
60	45361.5
61	37454.5
62	30584.5
63	23508.0
64	17084.5
65	12717.0
66	9828.0
67	7542.0
68	5937.5
69	4636.0
70	3575.5
71	2751.0
72	2092.5
73	1681.0
74	1321.0
75	986.5
76	742.0
77	459.0
78	244.0
79	133.5
80	89.0
81	62.5
82	31.0
83	13.5
84	7.5
85	4.0
86	3.5
87	2.5
88	2.5
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023273375506010226
2	0.0018569182584582628
3	0.030082075787023856
4	0.16644177323314227
5	0.02228301910149915
6	0.0629495289617351
7	0.047289518315403754
8	0.03998563983213459
9	0.030453459438715507
10-11	0.05877146288020401
12-13	0.05369588630708476
14-15	0.05261268398965077
16-17	0.0449993191299719
18-19	0.032527018160660566
20-21	0.021447405885192932
22-23	0.0025687369242005965
24-25	0.004332809269735946
26-27	0.002878223300610307
28-29	0.004394706545017888
30-31	0.001980712809022147
32-33	0.007984748511370529
34-35	0.00414711744389012
36-37	0.005787395238861586
38-39	0.001980712809022147
40-41	0.002878223300610307
42-43	0.003342452865224873
44-45	0.004270911994454004
46-47	0.0043018606320949756
48-49	0.0018878668960992339
50-51	0.001361740056202726
52-53	7.11818665742334E-4
54-55	0.004673244283786628
56-57	6.189727528194209E-4
58-59	5.880241151784498E-4
60-61	6.189727528194209E-5
62-63	9.594077668701024E-4
64-65	0.0
66-67	0.0036828878792755544
68-69	5.880241151784498E-4
70-71	0.0
72-73	0.002197353272508944
74-75	0.004951782022555367
76-77	0.0016093291573304942
78-79	1.8569182584582628E-4
80-81	0.0
82-83	0.004951782022555367
84-85	0.001516483244407581
86-87	0.0
88-89	0.0012069968679978707
90-91	5.880241151784498E-4
92-93	0.0
94-95	0.003002017851174191
96-97	0.0011760482303568996
98-99	6.499213904603919E-4
100-101	0.0
102-103	0.0015474318820485524
104-105	0.0
106-107	5.261268398965078E-4
108-109	0.0022901991854318575
110-111	9.284591292291314E-5
112-113	3.404350140506815E-4
114-115	0.0
116-117	0.0012998427809207838
118-119	0.0019497641713811758
120-121	0.0024139937359957415
122-123	0.0011141509550749577
124-125	0.09829287314772404
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1615580.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.137802929170924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.35723316695051	49.12864685294458
2	11.02445953118708	13.480224684366718
3	3.421209190154512	6.274956397414051
4	1.5968043957070341	3.9050044984468215
5	0.894155376533051	2.733334759926815
6	0.5756853965078577	2.1117684192539414
7	0.3996128400638208	1.7102015744651724
8	0.2862521902509021	1.4000663996482556
9	0.22405711407481435	1.2328523716666293
>10	1.135613115857982	12.112278721585177
>50	0.05868985822991224	2.434914522690021
>100	0.024398128330131753	2.518695529285066
>500	0.0014230970074656046	0.5867245966288485
>1k	4.065991449901727E-4	0.3703306716779269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2104	0.13023186719320617	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1716	0.10621572438381263	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	6.189727528194209E-5
7	0.0	0.0	0.0	0.0	6.189727528194209E-5
8	0.0	0.0	0.0	0.0	6.189727528194209E-5
9	0.0	0.0	0.0	0.0	6.189727528194209E-5
10-11	0.0	0.0	0.0	0.0	9.284591292291314E-5
12-13	0.0	0.0	0.0	0.0	2.4758910112776837E-4
14-15	0.0	0.0	0.0	0.0	3.0948637640971046E-4
16-17	0.0	0.0	0.0	0.0	3.0948637640971046E-4
18-19	0.0	0.0	0.0	6.189727528194209E-5	3.0948637640971046E-4
20-21	0.0	0.0	0.0	6.189727528194209E-5	3.404350140506815E-4
22-23	0.0	0.0	0.0	2.1664046348679732E-4	3.7138365169165255E-4
24-25	0.0	0.0	0.0	4.951782022555367E-4	4.023322893326236E-4
26-27	0.0	0.0	0.0	8.975104915881604E-4	4.951782022555367E-4
28-29	0.0	0.0	0.0	0.002754428750046423	4.951782022555367E-4
30-31	0.0	0.0	0.0	0.011760482303568997	4.951782022555367E-4
32-33	0.0	0.0	0.0	0.02937025712128152	4.951782022555367E-4
34-35	0.0	0.0	0.0	0.05224130033795912	4.951782022555367E-4
36-37	0.0	0.0	0.0	0.08362321890590377	4.951782022555367E-4
38-39	0.0	0.0	0.0	0.13589546788150386	5.261268398965078E-4
40-41	0.0	0.0	0.0	0.20240409017195063	6.189727528194209E-4
42-43	0.0	0.0	0.0	0.2636514440634323	6.49921390460392E-4
44-45	0.0	0.0	0.0	0.33452382426125604	6.80870028101363E-4
46-47	0.0	0.0	0.0	0.40886245187486847	7.427673033833051E-4
48-49	0.0	0.0	0.0	0.48230356899689275	9.284591292291314E-4
50-51	0.0	0.0	0.0	0.5597370603746024	9.284591292291314E-4
52-53	0.0	0.0	0.0	0.6427722551653277	9.903564045110735E-4
54-55	0.0	0.0	0.0	0.7408175392119238	0.0011141509550749577
56-57	0.0	0.0	0.0	0.8452691912502012	0.0011141509550749577
58-59	0.0	0.0	0.0	0.9602433800864086	0.0011141509550749577
60-61	0.0	0.0	0.0	1.071008554203444	0.0011760482303568996
62-63	0.0	0.0	0.0	1.1920486760172817	0.0011760482303568996
64-65	0.0	0.0	0.0	1.3202998304014657	0.0011760482303568996
66-67	0.0	0.0	0.0	1.4682343183253073	0.0012069968679978707
68-69	0.0	0.0	0.0	1.6285792099431782	0.0012379455056388418
70-71	0.0	0.0	0.0	1.7927926812661708	0.0012998427809207838
72-73	0.0	0.0	0.0	1.9481857908614861	0.0016093291573304944
74-75	0.0	0.0	0.0	2.1124302108221196	0.0016093291573304944
76-77	0.0	0.0	0.0	2.307870857524852	0.0016093291573304944
78-79	0.0	0.0	0.0	2.5264610851830303	0.0016093291573304944
80-81	0.0	0.0	0.0	2.740223325369217	0.0016712264326124364
82-83	0.0	0.0	0.0	2.954356949207096	0.0017950209831763206
84-85	0.0	0.0	0.0	3.1807771821884403	0.0017950209831763206
86-87	0.0	0.0	0.0	3.4464402876985356	0.0018259696208172917
88-89	0.0	0.0	0.0	3.752646108518303	0.0018569182584582628
90-91	0.0	0.0	0.0	4.0578925215712	0.0018569182584582628
92-93	0.0	0.0	0.0	4.3521521682615525	0.0018569182584582628
94-95	0.0	0.0	0.0	4.660029215513934	0.001980712809022147
96-97	0.0	0.0	0.0	5.01432921922777	0.0021045073595860313
98-99	0.0	0.0	0.0	5.410131346018148	0.0022283019101499153
100-101	0.0	0.0	0.0	5.830506691095458	0.0022283019101499153
102-103	3.0948637640971046E-5	0.0	0.0	6.249582193391847	0.002290199185431857
104-105	6.189727528194209E-5	0.0	0.0	6.668595798412953	0.0023520964607137993
106-107	6.189727528194209E-5	0.0	0.0	7.122426620780153	0.0024139937359957415
108-109	6.189727528194209E-5	0.0	0.0	7.62432067740378	0.0024758910112776837
110-111	6.189727528194209E-5	0.0	0.0	8.158958392651556	0.0025687369242005965
112-113	6.189727528194209E-5	0.0	0.0	8.688458634050928	0.0025996855618415676
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4340	0.0	43.42884	1
GGTATCA	2835	0.0	43.130222	1
GTACATG	8505	0.0	35.27561	1
TCAACGC	5410	0.0	34.383457	4
CAACGCA	5485	0.0	33.98827	5
ATGGGGG	4335	0.0	33.79927	5
TACATGG	8790	0.0	33.646427	2
ACATGGG	8880	0.0	33.03682	3
CATGGGG	7335	0.0	32.43135	4
AACGCAG	5760	0.0	32.383633	6
TATCAAC	5730	0.0	32.36323	2
ATCAACG	5725	0.0	32.287346	3
ACGCAGA	6885	0.0	27.090508	7
TACGGTC	90	0.009436909	26.497795	4
CGCAGAG	7225	0.0	25.814857	8
TATCACG	140	1.0409348E-4	25.554615	2
TGGGGGG	3120	0.0	25.021904	6
GCAGAGT	7960	0.0	23.13176	9
GAGTACT	3255	0.0	22.31457	12-13
GTCCTAA	415	1.8189894E-12	21.558773	1
>>END_MODULE