FastQCFastQC Report
Mon 15 Aug 2016
SRR2921592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740293
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG4050.074.939322
TACGGTC2550.051.339565
GTACGGT2900.051.302284
AGTACGG5850.042.725593
GGTATCA20600.040.162171
GTATCAA31900.038.6232871
CTAGTAC4250.036.4127961
GTACATG86600.033.1970021
TCAACGC36400.032.044194
TACATGG89550.031.6991372
CAACGCA37000.031.683575
ACATGGG89800.030.9481853
CATGGGG76950.029.6973174
AACGCAG40500.029.0907446
ATCAACG41100.028.6693613
TATCAAC42000.028.6217842
ATGGGGG47100.027.1635915
ACGCAGA49550.023.4118487
GAGTACA62300.023.1204851
TATTACG1602.608852E-422.316492
Produced by FastQC (version 0.11.5)