##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921592_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1740293
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.06977445751951	31.0	31.0	34.0	26.0	34.0
2	30.162172116994093	31.0	31.0	34.0	26.0	34.0
3	30.170714931336274	31.0	31.0	34.0	26.0	34.0
4	33.62978130694084	37.0	35.0	37.0	28.0	37.0
5	33.76846772353851	37.0	35.0	37.0	30.0	37.0
6	33.63291756043379	37.0	35.0	37.0	28.0	37.0
7	33.65548215156873	37.0	35.0	37.0	28.0	37.0
8	33.625607871777916	37.0	35.0	37.0	28.0	37.0
9	35.25815767804617	39.0	37.0	39.0	28.0	39.0
10-11	35.31157023558677	39.0	37.0	39.0	29.0	39.0
12-13	35.27070068086236	39.0	37.0	39.0	29.0	39.0
14-15	36.57483768537827	40.5	38.0	41.0	28.5	41.0
16-17	36.53588303808611	40.5	38.0	41.0	28.0	41.0
18-19	36.4829594786625	40.0	38.0	41.0	27.0	41.0
20-21	36.53217015755393	40.5	38.0	41.0	28.0	41.0
22-23	36.45052873280534	40.0	38.0	41.0	27.5	41.0
24-25	36.427768197654075	40.0	38.0	41.0	27.5	41.0
26-27	36.383836802193656	40.0	38.0	41.0	27.5	41.0
28-29	36.328216857736024	40.0	38.0	41.0	27.5	41.0
30-31	36.2336859367934	40.0	38.0	41.0	27.0	41.0
32-33	36.145776314678045	40.0	37.5	41.0	27.0	41.0
34-35	36.01936943951392	40.0	37.0	41.0	26.5	41.0
36-37	35.91457501696553	40.0	37.0	41.0	26.0	41.0
38-39	35.80378706344277	40.0	37.0	41.0	25.0	41.0
40-41	35.69804423737842	40.0	36.5	41.0	25.0	41.0
42-43	35.4470161059086	40.0	36.0	41.0	24.0	41.0
44-45	35.09756805319564	40.0	35.0	41.0	23.5	41.0
46-47	34.84018150966533	39.5	35.0	41.0	22.5	41.0
48-49	34.64950183675967	39.0	35.0	41.0	22.0	41.0
50-51	34.45335498102906	39.0	35.0	41.0	20.5	41.0
52-53	34.25071985004824	39.0	34.0	40.5	19.5	41.0
54-55	34.01341728088316	38.0	34.0	40.0	18.5	41.0
56-57	33.76677318129764	38.0	34.0	40.0	17.5	41.0
58-59	33.77169993788402	38.0	34.0	40.0	17.5	41.0
60-61	33.5649465348651	37.5	34.0	40.0	15.0	41.0
62-63	33.25993381574252	37.0	34.0	40.0	13.0	41.0
64-65	32.96096117148089	36.5	33.0	39.5	11.0	41.0
66-67	32.638195694633026	36.0	33.0	39.0	8.0	41.0
68-69	32.30744679200571	35.5	33.0	39.0	7.0	40.5
70-71	31.939368830421085	35.0	33.0	38.5	4.5	40.0
72-73	31.600309258268577	35.0	32.0	37.0	2.0	39.5
74-75	31.241586330577668	35.0	32.0	37.0	2.0	39.0
76-77	30.946236926770375	35.0	32.0	36.0	2.0	39.0
78-79	30.677850224071463	35.0	32.0	36.0	2.0	37.5
80-81	30.417726210471454	35.0	31.5	35.5	2.0	37.0
82-83	30.197588566982688	35.0	31.0	35.0	2.0	37.0
84-85	29.989764941880477	35.0	31.0	35.0	2.0	36.0
86-87	29.822167301712987	35.0	31.0	35.0	2.0	36.0
88-89	29.6617888481997	35.0	31.0	35.0	2.0	36.0
90-91	29.483124393421107	34.5	31.0	35.0	2.0	35.0
92-93	29.40357083548575	34.0	31.0	35.0	2.0	35.0
94-95	29.286108431166475	34.0	31.0	35.0	2.0	35.0
96-97	29.199895937063474	34.0	30.0	35.0	2.0	35.0
98-99	29.106821092770012	34.0	30.0	35.0	2.0	35.0
100-101	28.99524332971517	34.0	30.0	35.0	2.0	35.0
102-103	28.126071012180134	33.5	28.0	34.5	2.0	35.0
104-105	29.048901248238085	34.0	30.5	35.0	2.0	35.0
106-107	29.32582329527269	35.0	31.0	35.0	2.0	35.0
108-109	29.330694601426313	35.0	31.0	35.0	2.0	35.0
110-111	29.274170786183706	35.0	31.0	35.0	2.0	35.0
112-113	29.194315842217375	35.0	31.0	35.0	2.0	35.0
114-115	29.08032095744797	35.0	31.0	35.0	2.0	35.0
116-117	28.958400970411304	35.0	31.0	35.0	2.0	35.0
118-119	28.672022182471572	35.0	30.5	35.0	2.0	35.0
120-121	28.542050103057356	34.0	30.0	35.0	2.0	35.0
122-123	28.301005347950028	34.0	30.0	35.0	2.0	35.0
124-125	27.538777665599987	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78424.0
3	13300.0
4	6508.0
5	5809.0
6	6190.0
7	6252.0
8	6169.0
9	6579.0
10	6946.0
11	7328.0
12	7398.0
13	7321.0
14	7059.0
15	6247.0
16	5330.0
17	4974.0
18	4685.0
19	4549.0
20	4614.0
21	4638.0
22	5400.0
23	7122.0
24	9921.0
25	12454.0
26	14190.0
27	16344.0
28	19401.0
29	22965.0
30	27760.0
31	35367.0
32	46113.0
33	63570.0
34	98917.0
35	165057.0
36	334698.0
37	519639.0
38	140982.0
39	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.06712049732852	17.463744125950655	11.792642609503314	25.676492767217518
2	16.63792315742229	19.272364911732236	38.61213962621753	25.477572304627945
3	19.869734544194458	23.94492623865105	28.045690602877126	28.139648614277363
4	14.41946586227446	14.971671379962107	34.03428398040817	36.57457877735526
5	14.834797591058019	37.11343050096637	33.200020459570226	14.85175144840539
6	34.43900087884379	34.4456727296152	16.407921557440034	14.707404834100979
7	30.070033800896105	31.50382653281228	19.27013292106091	19.156006745230705
8	28.380869049316342	34.30579764801111	18.752155836510557	18.56117746616199
9	27.989566820742667	15.887262807831092	18.17960793960803	37.94356243181821
10-11	26.643494178228476	25.32380064837742	26.189549498999376	21.843155674394726
12-13	26.393027856677975	22.877072297482712	26.520264300091807	24.209635545747506
14-15	24.12180254103993	24.270699008707613	23.617491555779846	27.990006894472607
16-17	24.65108926826464	27.261223199402195	24.322181985399784	23.76550554693338
18-19	24.549638690588598	26.622385748707085	25.711818774177953	23.116156786526368
20-21	25.160143524925026	25.399054758753685	25.668631009603292	23.772170706717997
22-23	25.45476545915144	25.037665990470405	25.25239518910023	24.255173361277922
24-25	24.844609285074817	25.60834958348362	25.271141601199037	24.275899530242523
26-27	24.47247742022272	25.797772806997877	25.561900294280132	24.16784947849927
28-29	24.51993747675355	25.854420173922243	25.580665393130147	24.044976956194063
30-31	24.856094825965982	25.587676256896387	25.478788248026714	24.07744066911092
32-33	24.464853776178217	25.634828281597432	25.4308658874692	24.469452054755152
34-35	24.53976126912296	25.960603196721877	25.524911297981294	23.974724236173866
36-37	24.726234770188345	25.9392178119872	25.37075502627244	23.96379239155202
38-39	24.624725850724857	26.022772206666023	25.48309470276931	23.86940723983981
40-41	24.600539617507934	25.792446447115676	25.534106074189218	24.072907861187176
42-43	24.517302064247076	25.585461989944296	25.481692135446245	24.415543810362387
44-45	24.493413084176606	25.65651486132922	25.592089666537838	24.25798238795634
46-47	24.40316320483987	25.743484755566616	25.60143481077154	24.25191722882197
48-49	24.26641291708746	25.96973858083784	25.60049500710725	24.163353494967446
50-51	24.25185246069835	25.872085587808346	25.63012977786566	24.24593217362765
52-53	24.400977896740333	25.778534034272365	25.54006609165756	24.280421977329738
54-55	24.316759458982578	25.562946755200134	25.578005031303398	24.54228875451389
56-57	24.29841814313155	25.749947984141198	25.398424235596778	24.55320963713047
58-59	24.25783017932652	25.872817686384774	25.539251113157746	24.33010102113096
60-61	24.4744623926294	25.775439181325204	25.618877109694033	24.13122131635137
62-63	24.33466358946152	25.727998399899306	25.68127132138488	24.256066689254293
64-65	24.25844035969594	25.544624619377736	25.641468486373466	24.55546653455286
66-67	24.359437130057625	25.90875492442226	25.320324543424004	24.411483402096103
68-69	24.255239296179763	25.845757626352484	25.551775973911212	24.347227103556545
70-71	24.178753542705046	25.670340157172504	25.76309722964777	24.387809070474677
72-73	24.315622374133717	25.90582687990473	25.49770044519749	24.280850300764065
74-75	24.27079825437527	25.93769287440766	25.41833273758966	24.37317613362741
76-77	24.309419095662218	25.710307887488664	25.456572619866925	24.523700396982186
78-79	24.190726951225926	25.7858371001843	25.53538425903175	24.488051689558027
80-81	24.282432185843618	26.060895165542835	25.358694106034196	24.297978542579347
82-83	24.255430309495456	25.93719968917111	25.546220854043767	24.26114914728967
84-85	24.32594121688468	25.890529399746963	25.378453623681057	24.4050757596873
86-87	24.226746503315145	25.961403346437965	25.43119290309399	24.380657247152897
88-89	24.264978889372955	25.7638161668435	25.46299116836659	24.508213775416955
90-91	24.29033522500641	25.707078428374867	25.5204016768722	24.482184669746527
92-93	24.306382430960056	25.802401169553	25.619514846978888	24.271701552508055
94-95	24.3315521082332	25.524332155523737	25.674381477557684	24.469734258685378
96-97	24.276684450888794	25.647382753873433	25.590745652865433	24.48518714237234
98-99	24.21922813244001	25.74893587921633	25.411870915985745	24.619965072357914
100-101	24.122838901878122	25.829477509120796	25.55972545516349	24.48795813383759
102-103	23.93641947835861	25.806558199444495	25.659795019993183	24.597227302203716
104-105	23.892778581000478	25.851735305813317	25.675291286349015	24.58019482683719
106-107	23.93392659155025	25.81489205881939	25.692354059828997	24.558827289801364
108-109	23.887598050850524	25.907349309585232	25.683015515151652	24.522037124412584
110-111	23.776752391158816	26.13214079464909	25.570502193631427	24.52060462056066
112-113	23.68851573516787	26.219054591257994	25.66956204941612	24.42286762415802
114-115	23.71715795931445	26.335182415473096	25.556987504687356	24.3906721205251
116-117	23.625574796199476	26.14798337185421	25.668694423916577	24.557747408029734
118-119	23.74999675871998	26.075852867207495	25.603951307618793	24.570199066453732
120-121	23.653256502431805	26.33119995035259	25.62090868553881	24.394634861676796
122-123	23.71622982466272	26.359778299343006	25.52080145976971	24.403190416224565
124-125	23.591619523701375	26.24317354106597	25.620217731903615	24.54498920332904
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	928.0
1	733.0
2	478.5
3	351.5
4	242.0
5	199.0
6	197.0
7	216.0
8	256.5
9	284.5
10	282.5
11	280.0
12	288.5
13	302.5
14	361.0
15	429.5
16	446.0
17	492.5
18	569.5
19	623.5
20	704.0
21	778.0
22	883.5
23	1031.0
24	1233.0
25	1598.5
26	2107.5
27	2866.0
28	3834.5
29	5253.0
30	7209.5
31	9472.0
32	12545.0
33	15669.5
34	18808.5
35	22414.0
36	26430.0
37	30354.0
38	33744.5
39	37564.0
40	41435.5
41	44670.0
42	48186.0
43	53371.0
44	58295.5
45	63066.0
46	66722.0
47	69509.5
48	73551.5
49	76152.0
50	77738.5
51	78014.5
52	76114.5
53	74179.0
54	73303.5
55	71276.5
56	66988.5
57	61520.0
58	56943.0
59	52005.0
60	44366.0
61	36998.0
62	30643.0
63	23820.0
64	17872.0
65	13734.5
66	10747.5
67	8487.0
68	6634.5
69	5150.0
70	4028.5
71	3166.0
72	2402.5
73	1857.5
74	1443.5
75	1077.0
76	774.0
77	530.0
78	366.5
79	243.0
80	166.0
81	128.5
82	103.5
83	78.0
84	65.5
85	55.5
86	40.5
87	37.5
88	33.5
89	27.5
90	28.0
91	26.5
92	25.5
93	22.0
94	18.0
95	16.0
96	14.0
97	11.5
98	7.5
99	4.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025110714115381717
2	0.0109177017892964
3	0.008849084608166555
4	0.01034308590564922
5	0.01585939838866214
6	0.09452431285996094
7	0.05688697248107072
8	0.047635656754351136
9	0.026374869059405514
10-11	0.050221428230763435
12-13	0.012957588176243885
14-15	0.028357293857988283
16-17	0.034074721900277716
18-19	0.04122868965168509
20-21	0.030196064685659253
22-23	0.03792464832071381
24-25	0.03249452822024797
26-27	0.04562450116158601
28-29	0.04482003892447996
30-31	0.02482340617355813
32-33	0.029391602448553204
34-35	0.04372826874555032
36-37	0.00928004652090194
38-39	0.02097347975312203
40-41	0.04180330553533227
42-43	0.02212271152041639
44-45	0.01669259141995055
46-47	0.023674174406263772
48-49	0.029880025949653305
50-51	0.029449064036917917
52-53	0.02508198332119936
54-55	0.022180173108781107
56-57	0.025225637292111153
58-59	0.017841823187244907
60-61	0.0234443280528049
62-63	0.02312828931679895
64-65	0.021777941990228082
66-67	0.02844348624053536
68-69	0.022323827079692903
70-71	0.04614165545686847
72-73	0.0224674810506047
74-75	0.00982593161036676
76-77	0.01669259141995055
78-79	0.025541676028117105
80-81	0.019278362896362856
82-83	0.02465102140846398
84-85	0.012555357057690859
86-87	0.01858882383598624
88-89	0.023616712817899056
90-91	0.022237634697145826
92-93	0.008102083959425223
94-95	0.011406125290396503
96-97	0.015370974887562037
98-99	0.007125236957225019
100-101	0.010141970346372708
102-103	0.004280888333171483
104-105	0.00465438865754215
106-107	9.481162080178453E-4
108-109	0.01448032026790891
110-111	0.001608924474212101
112-113	0.0029305410066006125
114-115	0.013819512001714654
116-117	0.006378236308483686
118-119	0.27969428136526436
120-121	0.001206693355659076
122-123	0.0010630393847472811
124-125	0.3159812744175837
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1740293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.55714574987958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33211292276084	52.50569068100337
2	10.571799207960849	13.649703646135801
3	3.3752124375507653	6.536822438033134
4	1.5012524012427204	3.8766628029753205
5	0.8560035220806993	2.7630572068686985
6	0.5311783585178578	2.0574815226011487
7	0.3528932327631957	1.5947245903147864
8	0.2637630158323629	1.362222996521412
9	0.19560970550520548	1.1365203841551514
>10	0.9595085544747887	10.80010438160077
>50	0.04520052182957479	1.9913114398105891
>100	0.015110682989302722	1.5736235242359786
>500	2.665773688778834E-4	0.08943190017907757
>1k	8.885912295929433E-5	0.06264248556481881
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.746158836471789E-5
2	0.0	0.0	0.0	0.0	1.1492317672943578E-4
3	0.0	0.0	0.0	0.0	1.1492317672943578E-4
4	0.0	0.0	0.0	5.746158836471789E-5	1.1492317672943578E-4
5	0.0	0.0	0.0	5.746158836471789E-5	1.1492317672943578E-4
6	0.0	0.0	0.0	5.746158836471789E-5	2.2984635345887157E-4
7	0.0	0.0	0.0	5.746158836471789E-5	2.2984635345887157E-4
8	0.0	0.0	0.0	5.746158836471789E-5	2.2984635345887157E-4
9	0.0	0.0	0.0	5.746158836471789E-5	2.2984635345887157E-4
10-11	0.0	0.0	0.0	5.746158836471789E-5	2.2984635345887157E-4
12-13	0.0	0.0	0.0	5.746158836471789E-5	3.160387360059484E-4
14-15	2.8730794182358946E-5	0.0	0.0	5.746158836471789E-5	7.182698545589737E-4
16-17	5.746158836471789E-5	0.0	0.0	5.746158836471789E-5	9.768470022002043E-4
18-19	5.746158836471789E-5	0.0	0.0	5.746158836471789E-5	9.768470022002043E-4
20-21	5.746158836471789E-5	0.0	0.0	5.746158836471789E-5	0.001063039384747281
22-23	5.746158836471789E-5	0.0	0.0	2.2984635345887157E-4	0.001177962561476717
24-25	5.746158836471789E-5	0.0	0.0	8.044622371060505E-4	0.0013216165323885116
26-27	5.746158836471789E-5	0.0	0.0	0.001695116856759178	0.0013216165323885116
28-29	5.746158836471789E-5	0.0	0.0	0.004884235011001021	0.0013216165323885116
30-31	5.746158836471789E-5	0.0	0.0	0.017238476509415367	0.0013503473265708706
32-33	5.746158836471789E-5	0.0	0.0	0.04171711315278519	0.0014940012974826652
34-35	5.746158836471789E-5	0.0	0.0	0.07409671819630373	0.001752578445123896
36-37	5.746158836471789E-5	0.0	0.0	0.12158872097974308	0.0017813092393062547
38-39	5.746158836471789E-5	0.0	0.0	0.18962324160356905	0.0018387708276709725
40-41	5.746158836471789E-5	0.0	0.0	0.2665643084239263	0.0019536940044004086
42-43	5.746158836471789E-5	0.0	0.0	0.3424423358595363	0.0019536940044004086
44-45	5.746158836471789E-5	0.0	0.0	0.435185339480191	0.0019824247985827676
46-47	5.746158836471789E-5	0.0	0.0	0.540598623335266	0.0020686171811298442
48-49	5.746158836471789E-5	0.0	0.0	0.642018326798993	0.0021260787694945622
50-51	5.746158836471789E-5	0.0	0.0	0.7386974492226309	0.002154809563676921
52-53	5.746158836471789E-5	0.0	0.0	0.8404044606281815	0.002298463534588716
54-55	5.746158836471789E-5	0.0	0.0	0.961217450165001	0.0024133867113181515
56-57	5.746158836471789E-5	0.0	0.0	1.1005618019494419	0.0024133867113181515
58-59	5.746158836471789E-5	0.0	0.0	1.2373778438458352	0.0024421175055005105
60-61	5.746158836471789E-5	0.0	0.0	1.371263344735628	0.0024708482996828695
62-63	5.746158836471789E-5	0.0	0.0	1.507274924394915	0.0024708482996828695
64-65	5.746158836471789E-5	0.0	0.0	1.6629383672749358	0.0025283098880475876
66-67	5.746158836471789E-5	0.0	0.0	1.8480221433976922	0.0025283098880475876
68-69	5.746158836471789E-5	0.0	0.0	2.0419550041286154	0.0025283098880475876
70-71	5.746158836471789E-5	0.0	0.0	2.23505467182825	0.0025283098880475876
72-73	5.746158836471789E-5	0.0	0.0	2.4205406790695587	0.0029592718007829715
74-75	5.746158836471789E-5	0.0	0.0	2.6246155101468545	0.0029880025949653305
76-77	5.746158836471789E-5	0.0	0.0	2.8696029921398294	0.0030454641833300485
78-79	5.746158836471789E-5	0.0	0.0	3.1384944948925266	0.0030454641833300485
80-81	5.746158836471789E-5	0.0	0.0	3.407989344323054	0.0030741949775124075
82-83	5.746158836471789E-5	0.0	0.0	3.672456304771668	0.003246579742606561
84-85	5.746158836471789E-5	0.0	0.0	3.9459447345935423	0.00327531053678892
86-87	5.746158836471789E-5	0.0	0.0	4.2749697895699175	0.003332772125153638
88-89	5.746158836471789E-5	0.0	0.0	4.642436647162288	0.003390233713518356
90-91	5.746158836471789E-5	0.0	0.0	5.0159657023271365	0.003390233713518356
92-93	5.746158836471789E-5	0.0	0.0	5.385989600601738	0.003390233713518356
94-95	5.746158836471789E-5	0.0	0.0	5.769574433730412	0.003447695301883074
96-97	5.746158836471789E-5	0.0	0.0	6.20131207790872	0.003476426096065433
98-99	5.746158836471789E-5	0.0	0.0	6.686948691973133	0.0035051568902477914
100-101	5.746158836471789E-5	0.0	0.0	7.203499640577764	0.0035051568902477914
102-103	5.746158836471789E-5	0.0	0.0	7.715195084965577	0.0035626184786125095
104-105	5.746158836471789E-5	0.0	0.0	8.22177644798893	0.0035626184786125095
106-107	5.746158836471789E-5	0.0	0.0	8.78214185772166	0.003620080066977227
108-109	5.746158836471789E-5	0.0	0.0	9.402755742854795	0.003735003243706663
110-111	5.746158836471789E-5	0.0	0.0	10.05330711552595	0.004051041979712611
112-113	5.746158836471789E-5	0.0	0.0	10.691188207962682	0.00407977277389497
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	405	0.0	74.93932	2
TACGGTC	255	0.0	51.33956	5
GTACGGT	290	0.0	51.30228	4
AGTACGG	585	0.0	42.72559	3
GGTATCA	2060	0.0	40.16217	1
GTATCAA	3190	0.0	38.623287	1
CTAGTAC	425	0.0	36.412796	1
GTACATG	8660	0.0	33.197002	1
TCAACGC	3640	0.0	32.04419	4
TACATGG	8955	0.0	31.699137	2
CAACGCA	3700	0.0	31.68357	5
ACATGGG	8980	0.0	30.948185	3
CATGGGG	7695	0.0	29.697317	4
AACGCAG	4050	0.0	29.090744	6
ATCAACG	4110	0.0	28.669361	3
TATCAAC	4200	0.0	28.621784	2
ATGGGGG	4710	0.0	27.163591	5
ACGCAGA	4955	0.0	23.411848	7
GAGTACA	6230	0.0	23.120485	1
TATTACG	160	2.608852E-4	22.31649	2
>>END_MODULE