FastQCFastQC Report
Mon 15 Aug 2016
SRR2921592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740293
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25680.14756135892059555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21230.1219909520982961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28850.046.31021
GTATCAA44150.043.2308431
GTACATG87900.034.2670671
TCAACGC55650.033.643514
CAACGCA56900.032.772255
TACATGG92650.032.0513082
GTATACG750.003864689531.8106941
ATCAACG58900.031.7880343
AACGCAG59500.031.3573346
TATCAAC60050.031.180172
ACATGGG93100.031.1269023
CATGGGG77800.030.42624
ATGGGGG43850.027.8521355
ACGCAGA71100.026.3236927
CGCAGAG74750.024.9571448
GCAGAGT84400.022.3154859
ATGGGGA32300.021.5803015
AGAGTAC76800.021.41951210-11
GAGTACT34800.021.04536412-13
GTAAGTA6350.020.6590712
Produced by FastQC (version 0.11.5)