##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1740293
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70664652446456	33.0	31.0	34.0	30.0	34.0
2	31.8830972715514	33.0	31.0	34.0	30.0	34.0
3	31.95656823305041	34.0	31.0	34.0	30.0	34.0
4	35.345854404976635	37.0	35.0	37.0	33.0	37.0
5	35.18825278272107	37.0	35.0	37.0	33.0	37.0
6	35.2133864814718	37.0	35.0	37.0	33.0	37.0
7	35.09719397825538	37.0	35.0	37.0	33.0	37.0
8	35.1389243075735	37.0	36.0	37.0	33.0	37.0
9	36.86277080928326	39.0	38.0	39.0	34.0	39.0
10-11	36.82096290682087	39.0	38.0	39.0	33.5	39.0
12-13	36.752143748207914	39.0	38.0	39.0	33.0	39.0
14-15	38.17849867809616	41.0	39.0	41.0	33.0	41.0
16-17	38.18539895293493	41.0	39.0	41.0	33.5	41.0
18-19	38.194304924515585	41.0	39.0	41.0	34.0	41.0
20-21	38.132810681879434	41.0	39.0	41.0	34.0	41.0
22-23	38.13784661548371	41.0	39.0	41.0	34.0	41.0
24-25	38.10123324060948	41.0	39.0	41.0	34.0	41.0
26-27	38.02782175185443	41.0	39.0	41.0	33.0	41.0
28-29	37.9567618786032	41.0	39.0	41.0	33.0	41.0
30-31	37.849118223195745	41.0	38.0	41.0	33.0	41.0
32-33	37.732703343632366	41.0	38.0	41.0	33.0	41.0
34-35	37.60084278911654	41.0	38.0	41.0	33.0	41.0
36-37	37.52455046362883	40.5	38.0	41.0	32.5	41.0
38-39	37.44207009969011	40.0	38.0	41.0	32.0	41.0
40-41	37.3257457221284	40.0	38.0	41.0	32.0	41.0
42-43	37.17502311392392	40.0	38.0	41.0	31.5	41.0
44-45	37.02447691279572	40.0	37.0	41.0	31.0	41.0
46-47	36.831627490313416	40.0	37.0	41.0	31.0	41.0
48-49	36.64694824377274	40.0	37.0	41.0	30.0	41.0
50-51	36.411029062347545	40.0	36.0	41.0	30.0	41.0
52-53	36.281866329405446	40.0	36.0	41.0	30.0	41.0
54-55	36.15704108446107	40.0	35.0	41.0	29.5	41.0
56-57	35.960732474359205	39.0	35.0	41.0	29.0	41.0
58-59	35.76963563032202	39.0	35.0	41.0	29.0	41.0
60-61	35.52515840723372	39.0	35.0	41.0	29.0	41.0
62-63	35.17893251308831	38.0	35.0	41.0	28.0	41.0
64-65	34.89409685610411	37.0	35.0	40.0	28.0	41.0
66-67	34.594492134370476	37.0	35.0	39.5	28.0	41.0
68-69	34.2304744086197	36.0	35.0	39.0	28.0	41.0
70-71	33.85512410841163	36.0	35.0	39.0	27.0	41.0
72-73	33.53605312438768	35.0	34.0	38.0	27.0	40.0
74-75	33.210894947000305	35.0	34.0	37.0	26.5	39.0
76-77	32.88229913008901	35.0	34.0	37.0	26.0	39.0
78-79	32.62060555320282	35.0	34.0	36.0	26.0	38.5
80-81	32.362277501547155	35.0	34.0	36.0	26.0	37.0
82-83	32.136337674173255	35.0	34.0	35.5	26.0	37.0
84-85	31.92331808494317	35.0	34.0	35.0	25.5	36.5
86-87	31.792384098539728	35.0	34.0	35.0	25.0	36.0
88-89	31.687429358159804	35.0	34.0	35.0	25.0	36.0
90-91	31.581407843391887	35.0	34.0	35.0	25.0	36.0
92-93	31.502294728531346	35.0	33.0	35.0	24.5	35.0
94-95	31.436667273844115	35.0	33.0	35.0	24.0	35.0
96-97	31.36946623355952	35.0	33.0	35.0	24.0	35.0
98-99	31.290812811405896	35.0	33.0	35.0	23.5	35.0
100-101	31.222943780156562	35.0	33.0	35.0	23.0	35.0
102-103	30.540508408641536	34.0	31.5	35.0	21.5	35.0
104-105	31.055428597368376	35.0	33.0	35.0	23.0	35.0
106-107	31.15248208204021	35.0	33.0	35.0	23.0	35.0
108-109	31.137774213882373	35.0	33.0	35.0	23.0	35.0
110-111	31.062338353369235	35.0	33.0	35.0	20.0	35.0
112-113	31.025550869882256	35.0	33.0	35.0	20.0	35.0
114-115	30.895896265743758	35.0	33.0	35.0	19.0	35.0
116-117	31.071354076583656	35.0	33.0	35.0	20.0	35.0
118-119	31.045293235104662	35.0	34.0	35.0	19.5	35.0
120-121	30.9610154726819	35.0	33.0	35.0	18.0	35.0
122-123	30.817691043979373	35.0	33.0	35.0	6.0	35.0
124-125	30.175047822406917	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	29500.0
3	8075.0
4	4256.0
5	3340.0
6	3630.0
7	3979.0
8	4172.0
9	4456.0
10	4751.0
11	5121.0
12	5655.0
13	7054.0
14	9244.0
15	8396.0
16	4946.0
17	4343.0
18	4042.0
19	3650.0
20	3574.0
21	3695.0
22	3975.0
23	5015.0
24	6415.0
25	8328.0
26	10254.0
27	11864.0
28	14549.0
29	17370.0
30	21707.0
31	26887.0
32	34780.0
33	47813.0
34	73191.0
35	129235.0
36	304600.0
37	649009.0
38	249080.0
39	342.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.89235560904592	17.769934311448456	11.113314331990134	27.224395747515484
2	16.084061147290313	19.724270610605217	37.05900910038	27.132659141724467
3	18.896312611796514	23.20533542403355	28.558750362117248	29.33960160205269
4	13.473028042547314	14.974904452732881	33.88710457245476	37.66496293226504
5	14.678662854922678	37.21973738509305	32.874625119117	15.226974640867274
6	35.03058467971475	33.64291768299344	16.259963452486506	15.066534184805297
7	30.472229584330574	31.551747828401616	19.194865777426184	18.78115680984163
8	28.238815717236456	34.27669864680828	18.55147147167863	18.933014164276635
9	27.976550074236638	15.026093474360913	18.369530041552977	38.627826409849476
10-11	26.920305937984423	24.87655073437076	26.206577137678995	21.996566189965822
12-13	26.824757086184096	22.34088426378428	26.7487207497269	24.08563790030472
14-15	24.20602020610875	23.72147951216483	24.019735798113697	28.052764483612723
16-17	24.301777148192265	26.80597970813193	24.90436445335676	23.98787869031905
18-19	24.320594144207583	25.92836469509584	26.665325396848203	23.085715763848373
20-21	25.013398962625732	24.796256739354902	26.614751014944478	23.57559328307489
22-23	24.90285833809321	24.543971470634542	26.319385464780186	24.233784726492065
24-25	24.331890978674988	25.0087345780107	26.39382603249033	24.265548410823982
26-27	24.10459521931696	25.230278138122348	26.588746043991247	24.076380598569443
28-29	24.078610030223356	25.427527302013175	26.554316273807103	23.939546393956363
30-31	24.50798933720709	25.02782634978627	26.416902790794694	24.04728152221195
32-33	24.216814088094264	25.06864406000451	26.34386047987054	24.370681372030692
34-35	24.336135583753325	25.223845303594366	26.505042355556817	23.934976757095487
36-37	24.336770243265416	25.315291112544553	26.390784848097393	23.95715379609264
38-39	24.364835558865412	25.368537308835815	26.486158948845517	23.78046818345326
40-41	24.35755840012504	25.141791113389218	26.484827345256623	24.01582314122912
42-43	24.245165950431364	24.99161750420274	26.343821102168185	24.419395443197708
44-45	24.27146110029666	25.1849702263373	26.208753241342652	24.334815432023387
46-47	24.310333285158976	25.26050787650776	26.082824078636648	24.34633475969662
48-49	24.10868537755297	25.503799017671287	26.12652474281749	24.260990861958252
50-51	24.187834125691452	25.46142996817447	26.096159244300953	24.254576661833124
52-53	24.372592516189236	25.34651821133021	25.978486751961544	24.30240252051901
54-55	24.134714013578858	25.19200324099747	26.020095333595755	24.65318741182792
56-57	24.198282056578876	25.340225716745913	25.86807226544487	24.593419961230342
58-59	24.126224624392016	25.432998224134817	26.019973239915334	24.420803911557833
60-61	24.34457101008653	25.322884126021417	26.093502151794684	24.23904271209737
62-63	24.17334256941354	25.247855728673002	26.199026419077658	24.3797752828358
64-65	24.04135395591432	25.04882798471292	26.17019088165039	24.739627177722372
66-67	24.09619341888942	25.454927328279194	25.850824865548837	24.59805438728255
68-69	24.0761900602748	25.41643454399135	25.964047729114775	24.54332766661907
70-71	23.917639156165084	25.25640797268046	26.17470161633702	24.651251254817435
72-73	24.08258337075604	25.398512343172992	25.894675689490064	24.6242285965809
74-75	23.888639076886385	25.46242596027175	25.92656867970446	24.722366283137404
76-77	23.915112757479264	25.135015628398378	26.01749794499275	24.93237366912961
78-79	23.811678621563864	25.238911193587736	26.059319964304823	24.890090220543577
80-81	23.870664307676925	25.554633616293348	25.858576687948524	24.716125388081203
82-83	23.85092585914965	25.4514653390756	25.935089439714933	24.762519362059816
84-85	23.841555074402503	25.383074062326177	25.770601471960575	25.004769391310745
86-87	23.682966029283577	25.533861252099506	25.841654250175118	24.94151846844181
88-89	23.673204240597816	25.3460160141495	25.851425077724855	25.129354667527835
90-91	23.702333765065124	25.266940109568704	25.98992804597886	25.040798079387315
92-93	23.6404157230995	25.39572359367072	26.00622423925167	24.957636443978114
94-95	23.629734447165976	25.13507495384969	26.066298421910805	25.16889217707353
96-97	23.60021720767467	25.182299298384734	26.025817832865016	25.19166566107558
98-99	23.469534635097407	25.24307158706403	25.882049406431552	25.405344371407008
100-101	23.394566317281054	25.226010792435527	26.048975660995016	25.3304472292884
102-103	23.217153709348864	25.155105687926838	26.200176409914583	25.427564192809715
104-105	23.242637877644743	25.205554466977688	26.121233608363646	25.43057404701392
106-107	23.276808953034323	25.209025091630288	26.051791046448518	25.462374908886865
108-109	23.30597517941398	25.172317294823905	26.03992302251	25.48178450325212
110-111	23.109015420126696	25.407396064047926	25.947823078962966	25.535765436862405
112-113	22.955970506031186	25.445903206825538	26.024916565533978	25.573209721609295
114-115	22.893242689593073	25.542193182412387	25.97763709904022	25.58692702895432
116-117	22.746384302566458	25.40469550796749	26.1201062600452	25.728813929420852
118-119	22.909830931560094	25.29016079372869	25.988885453862164	25.811122820849054
120-121	22.73835919221788	25.352500349806768	26.051481787206583	25.857658670768767
122-123	22.65536112828599	25.472455966552275	25.925259787572948	25.94692311758878
124-125	22.38233933060663	25.420819109977682	26.01791963057479	26.178921928840897
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	41.5
2	34.5
3	71.5
4	64.0
5	67.0
6	178.5
7	474.5
8	797.0
9	855.0
10	705.0
11	533.5
12	446.0
13	454.5
14	499.0
15	533.0
16	589.0
17	649.5
18	723.0
19	777.0
20	828.5
21	922.5
22	995.5
23	1142.5
24	1334.5
25	1595.0
26	2089.5
27	2862.5
28	3845.5
29	5219.0
30	7013.0
31	9246.5
32	12330.5
33	15328.0
34	18429.5
35	22000.5
36	25848.0
37	29785.0
38	33052.0
39	36837.5
40	40852.5
41	44342.0
42	47996.5
43	52973.5
44	58132.5
45	63003.0
46	66601.0
47	69899.0
48	74495.0
49	77188.5
50	78795.0
51	79217.5
52	77194.5
53	75102.5
54	74371.0
55	72183.5
56	67844.0
57	62351.5
58	57527.0
59	52292.5
60	44569.0
61	37331.0
62	30623.5
63	23445.0
64	17277.5
65	13086.5
66	10141.0
67	7922.5
68	6106.5
69	4673.5
70	3641.0
71	2784.0
72	2103.5
73	1632.5
74	1188.5
75	826.0
76	568.5
77	322.5
78	174.5
79	109.0
80	64.0
81	34.0
82	25.5
83	15.5
84	6.5
85	5.0
86	4.5
87	2.5
88	4.5
89	5.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023904020759722644
2	0.0020686171811298442
3	0.030167333891476893
4	0.16853483867371757
5	0.023386866464440183
6	0.06820690538892014
7	0.05206019905843441
8	0.044590192571021085
9	0.03367249078172469
10-11	0.06418459420338989
12-13	0.05705935724616487
14-15	0.058438435366918104
16-17	0.050393812995857594
18-19	0.0372351092603372
20-21	0.02410513631899916
22-23	0.0029305410066006125
24-25	0.004769311834271586
26-27	0.0034764260960654324
28-29	0.004740581040089226
30-31	0.0020686171811298442
32-33	0.00902146937326071
34-35	0.004855504216818662
36-37	0.0066368134561249174
38-39	0.002011155592765126
40-41	0.00327531053678892
42-43	0.0034189645077007144
44-45	0.00462565786335979
46-47	0.004970427393548098
48-49	0.002327194328771075
50-51	0.0013503473265708706
52-53	9.481162080178453E-4
54-55	0.005056619776095175
56-57	8.331930312884096E-4
58-59	8.331930312884096E-4
60-61	8.619238254707684E-5
62-63	9.481162080178453E-4
64-65	0.0
66-67	0.004022311185530252
68-69	7.757314429236915E-4
70-71	0.0
72-73	0.002269732740406357
74-75	0.005487581688830559
76-77	0.0018100400334886137
78-79	1.1492317672943578E-4
80-81	0.0
82-83	0.005659966453924713
84-85	0.0016663860625768191
86-87	0.0
88-89	0.001120500973111999
90-91	8.619238254707684E-4
92-93	0.0
94-95	0.003189118154241843
96-97	0.0013216165323885116
98-99	7.757314429236915E-4
100-101	0.0
102-103	0.001608924474212101
104-105	0.0
106-107	6.608082661942558E-4
108-109	0.0025283098880475876
110-111	1.4365397091179474E-4
112-113	2.873079418235895E-4
114-115	0.0
116-117	0.0019536940044004086
118-119	0.0029305410066006125
120-121	0.002671963858959382
122-123	0.0014365397091179472
124-125	0.10391928255759231
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1740293.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.36859231492049
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.97936780246827	48.46852623959834
2	11.846698483605099	14.540304190362964
3	3.7965466110599326	6.989661635361901
4	1.7577723271735943	4.314880533150613
5	0.9502440873589372	2.9157570998397153
6	0.5856198713981707	2.1563200283610304
7	0.4047254925042382	1.73862036242636
8	0.29010081176702396	1.4242462758046386
9	0.2332861787633791	1.2884799957511925
>10	1.083503094577605	11.651434398008917
>50	0.052761298159013696	2.196647166074209
>100	0.01871578396986377	1.8164187457780372
>500	3.7608982559834286E-4	0.1415387327570793
>1k	2.820673691987571E-4	0.35716459672495754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2568	0.14756135892059555	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2123	0.1219909520982961	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.746158836471789E-5
2	0.0	0.0	0.0	0.0	5.746158836471789E-5
3	0.0	0.0	0.0	0.0	5.746158836471789E-5
4	0.0	0.0	0.0	0.0	5.746158836471789E-5
5	0.0	0.0	0.0	0.0	5.746158836471789E-5
6	0.0	0.0	0.0	0.0	1.7238476509415368E-4
7	0.0	0.0	0.0	0.0	1.7238476509415368E-4
8	0.0	0.0	0.0	0.0	1.7238476509415368E-4
9	0.0	0.0	0.0	0.0	2.2984635345887157E-4
10-11	0.0	0.0	0.0	2.8730794182358946E-5	2.2984635345887157E-4
12-13	0.0	0.0	0.0	5.746158836471789E-5	2.873079418235895E-4
14-15	0.0	0.0	0.0	5.746158836471789E-5	4.3096191273538423E-4
16-17	0.0	0.0	0.0	5.746158836471789E-5	5.171542952824611E-4
18-19	0.0	0.0	0.0	5.746158836471789E-5	6.03346677829538E-4
20-21	0.0	0.0	0.0	5.746158836471789E-5	7.757314429236916E-4
22-23	0.0	0.0	0.0	2.2984635345887157E-4	9.481162080178453E-4
24-25	0.0	0.0	0.0	8.619238254707685E-4	0.001149231767294358
26-27	0.0	0.0	0.0	0.001752578445123896	0.0012066933556590758
28-29	0.0	0.0	0.0	0.004970427393548098	0.0012066933556590758
30-31	0.0	0.0	0.0	0.017468322862874242	0.0012066933556590758
32-33	0.0	0.0	0.0	0.04234919062479709	0.0012066933556590758
34-35	0.0	0.0	0.0	0.07538960393450989	0.0012066933556590758
36-37	0.0	0.0	0.0	0.12291033751213158	0.0012066933556590758
38-39	0.0	0.0	0.0	0.19172058957888127	0.0013216165323885116
40-41	0.0	0.0	0.0	0.2680295789272266	0.0013216165323885116
42-43	0.0	0.0	0.0	0.34422364509884257	0.0013216165323885116
44-45	0.0	0.0	0.0	0.43647822521839713	0.0013503473265708706
46-47	0.0	0.0	0.0	0.5423224709862076	0.0013790781207532294
48-49	0.0	0.0	0.0	0.6436272512732051	0.0014940012974826652
50-51	0.0	0.0	0.0	0.7397604886073781	0.0014940012974826652
52-53	0.0	0.0	0.0	0.8414100384245642	0.001580193680029742
54-55	0.0	0.0	0.0	0.9623092203439306	0.001723847650941537
56-57	0.0	0.0	0.0	1.1014237257749127	0.001752578445123896
58-59	0.0	0.0	0.0	1.2392453454676886	0.0018387708276709725
60-61	0.0	0.0	0.0	1.3735618082702166	0.0018962324160356906
62-63	0.0	0.0	0.0	1.5110673892269864	0.0018962324160356906
64-65	0.0	0.0	0.0	1.669517719142696	0.002011155592765126
66-67	0.0	0.0	0.0	1.8574171130953236	0.002039886386947485
68-69	0.0	0.0	0.0	2.0531025522713704	0.0020686171811298442
70-71	0.0	0.0	0.0	2.2491902225659706	0.002327194328771075
72-73	0.0	0.0	0.0	2.4370608857244154	0.002873079418235895
74-75	0.0	0.0	0.0	2.6438364114548527	0.0029305410066006125
76-77	0.0	0.0	0.0	2.892616358279899	0.0029305410066006125
78-79	0.0	0.0	0.0	3.1664208268377796	0.0029305410066006125
80-81	0.0	0.0	0.0	3.438214139802895	0.0029592718007829715
82-83	0.0	0.0	0.0	3.7072205657323223	0.003160387360059484
84-85	0.0	0.0	0.0	3.9873458090103218	0.003160387360059484
86-87	0.0	0.0	0.0	4.323180062207916	0.003160387360059484
88-89	0.0	0.0	0.0	4.6965941941960345	0.003160387360059484
90-91	0.0	0.0	0.0	5.077018639964649	0.003160387360059484
92-93	0.0	0.0	0.0	5.452989812634999	0.003160387360059484
94-95	0.0	0.0	0.0	5.844849114488193	0.003390233713518356
96-97	0.0	0.0	0.0	6.281643378442595	0.003418964507700715
98-99	0.0	0.0	0.0	6.774807460582787	0.003476426096065433
100-101	0.0	0.0	0.0	7.297276952788984	0.0035051568902477914
102-103	2.8730794182358946E-5	0.0	0.0	7.814431248071445	0.0035913492727948685
104-105	5.746158836471789E-5	0.0	0.0	8.323627113365394	0.0036200800669772275
106-107	5.746158836471789E-5	0.0	0.0	8.889077873668398	0.003648810861159586
108-109	5.746158836471789E-5	0.0	0.0	9.518943074528256	0.003792464832071381
110-111	5.746158836471789E-5	0.0	0.0	10.182021073462916	0.003964849597165534
112-113	5.746158836471789E-5	5.746158836471789E-5	0.0	10.833003408046807	0.003964849597165534
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2885	0.0	46.3102	1
GTATCAA	4415	0.0	43.230843	1
GTACATG	8790	0.0	34.267067	1
TCAACGC	5565	0.0	33.64351	4
CAACGCA	5690	0.0	32.77225	5
TACATGG	9265	0.0	32.051308	2
GTATACG	75	0.0038646895	31.810694	1
ATCAACG	5890	0.0	31.788034	3
AACGCAG	5950	0.0	31.357334	6
TATCAAC	6005	0.0	31.18017	2
ACATGGG	9310	0.0	31.126902	3
CATGGGG	7780	0.0	30.4262	4
ATGGGGG	4385	0.0	27.852135	5
ACGCAGA	7110	0.0	26.323692	7
CGCAGAG	7475	0.0	24.957144	8
GCAGAGT	8440	0.0	22.315485	9
ATGGGGA	3230	0.0	21.580301	5
AGAGTAC	7680	0.0	21.419512	10-11
GAGTACT	3480	0.0	21.045364	12-13
GTAAGTA	635	0.0	20.659071	2
>>END_MODULE