FastQCFastQC Report
Mon 15 Aug 2016
SRR2921591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428178
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG88000.035.7126661
TACATGG89700.034.565282
GTATCAA35900.033.9883731
CATGGGG73950.033.5577434
TAACGCA1251.2765158E-633.3259774
ACATGGG94650.032.5060963
GGTATCA25700.031.265951
ATGGGGG48250.030.3390675
TCAACGC42850.027.359634
TATCAAC44450.027.1781042
CAACGCA43200.027.1360665
ATCAACG44650.026.5232323
AACGCAG44800.026.2988246
GTATAGA4900.025.5090451
TGGGGGG40000.023.5042466
ATGGGGA25400.022.4906465
TATAACG1602.6085236E-422.3165042
ACGCAGA52700.022.1267977
CGCAGAG56200.020.8531958
GTCTTAT4152.910383E-1120.079411
Produced by FastQC (version 0.11.5)