##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921591_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1428178
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.6475817440123	31.0	31.0	34.0	26.0	34.0
2	30.012170751825053	31.0	31.0	34.0	26.0	34.0
3	30.085174256990374	31.0	31.0	34.0	26.0	34.0
4	33.52823457580217	37.0	35.0	37.0	28.0	37.0
5	33.5744283975807	37.0	35.0	37.0	28.0	37.0
6	33.54189673836174	37.0	35.0	37.0	28.0	37.0
7	33.47816798746375	37.0	35.0	37.0	28.0	37.0
8	33.39873531170484	37.0	35.0	37.0	27.0	37.0
9	35.1098651568642	39.0	37.0	39.0	28.0	39.0
10-11	35.13799295325933	39.0	37.0	39.0	27.5	39.0
12-13	35.06749228737594	39.0	37.0	39.0	27.0	39.0
14-15	36.35179368398057	40.0	38.0	41.0	27.0	41.0
16-17	36.32512158848547	40.0	38.0	41.0	27.0	41.0
18-19	36.28787973207821	40.0	38.0	41.0	27.0	41.0
20-21	36.35294340061253	40.0	38.0	41.0	27.0	41.0
22-23	36.296103496903044	40.0	38.0	41.0	27.0	41.0
24-25	36.30368413461068	40.0	37.5	41.0	27.0	41.0
26-27	36.276999785741	40.0	37.0	41.0	27.0	41.0
28-29	36.223367465399974	40.0	37.0	41.0	27.0	41.0
30-31	36.12962109765029	40.0	37.0	41.0	27.0	41.0
32-33	36.031753744981366	40.0	37.0	41.0	27.0	41.0
34-35	35.90804227484249	40.0	37.0	41.0	26.5	41.0
36-37	35.80495533469917	40.0	36.5	41.0	26.0	41.0
38-39	35.68310952836411	40.0	36.0	41.0	25.0	41.0
40-41	35.570481060484056	40.0	36.0	41.0	25.0	41.0
42-43	35.331787424256646	40.0	35.5	41.0	24.0	41.0
44-45	35.00515972098716	40.0	35.0	41.0	23.0	41.0
46-47	34.758090378090124	39.0	35.0	41.0	22.5	41.0
48-49	34.56600577799126	39.0	35.0	41.0	21.5	41.0
50-51	34.36963039621112	39.0	34.5	41.0	20.0	41.0
52-53	34.18009274754267	39.0	34.0	40.0	19.5	41.0
54-55	33.94941982021849	38.0	34.0	40.0	18.0	41.0
56-57	33.70077434325413	38.0	34.0	40.0	16.5	41.0
58-59	33.71660255234292	38.0	34.0	40.0	17.5	41.0
60-61	33.50460096710634	37.5	34.0	40.0	14.0	41.0
62-63	33.18544887261952	37.0	33.0	40.0	12.5	41.0
64-65	32.88461802380375	36.5	33.0	39.5	8.0	41.0
66-67	32.55438712821511	36.0	33.0	39.0	7.5	41.0
68-69	32.20898025316172	35.5	33.0	39.0	4.0	40.5
70-71	31.839374013603347	35.0	32.5	38.0	2.0	40.0
72-73	31.48514996029907	35.0	32.0	37.0	2.0	39.5
74-75	31.11506863990343	35.0	32.0	37.0	2.0	39.0
76-77	30.81409740242463	35.0	32.0	36.0	2.0	39.0
78-79	30.540273341278187	35.0	31.0	36.0	2.0	37.5
80-81	30.291188843407475	35.0	31.0	35.5	2.0	37.0
82-83	30.07549304078343	35.0	31.0	35.0	2.0	37.0
84-85	29.864513737083193	35.0	31.0	35.0	2.0	36.0
86-87	29.692627949737357	35.0	31.0	35.0	2.0	36.0
88-89	29.542032925867787	35.0	31.0	35.0	2.0	36.0
90-91	29.365634745808997	34.0	31.0	35.0	2.0	35.0
92-93	29.290858702486666	34.0	30.0	35.0	2.0	35.0
94-95	29.16453901404447	34.0	30.0	35.0	2.0	35.0
96-97	29.082303816471054	34.0	30.0	35.0	2.0	35.0
98-99	28.997572431447622	34.0	30.0	35.0	2.0	35.0
100-101	28.89596499876066	34.0	30.0	35.0	2.0	35.0
102-103	28.04512497741878	33.5	28.0	34.5	2.0	35.0
104-105	28.937934557177044	34.0	30.5	35.0	2.0	35.0
106-107	29.198479111147208	35.0	31.0	35.0	2.0	35.0
108-109	29.199463582270557	35.0	31.0	35.0	2.0	35.0
110-111	29.14411298871709	35.0	31.0	35.0	2.0	35.0
112-113	29.07182753130212	35.0	31.0	35.0	2.0	35.0
114-115	28.959377962690926	35.0	31.0	35.0	2.0	35.0
116-117	28.846074859016174	35.0	31.0	35.0	2.0	35.0
118-119	28.56879149517777	34.5	30.0	35.0	2.0	35.0
120-121	28.454279858673075	34.0	30.0	35.0	2.0	35.0
122-123	28.226649619305157	34.0	30.0	35.0	2.0	35.0
124-125	27.47631072597393	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62898.0
3	10934.0
4	5427.0
5	4586.0
6	4750.0
7	5130.0
8	5412.0
9	5664.0
10	6161.0
11	6786.0
12	6960.0
13	7149.0
14	7139.0
15	6115.0
16	4904.0
17	4431.0
18	3937.0
19	3843.0
20	3685.0
21	3911.0
22	4444.0
23	6212.0
24	9571.0
25	11838.0
26	12506.0
27	13334.0
28	16198.0
29	19303.0
30	23216.0
31	29369.0
32	37950.0
33	52376.0
34	82846.0
35	135008.0
36	267378.0
37	421596.0
38	115116.0
39	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87850420161005	17.72674616504154	12.487375559082384	24.90737407426603
2	16.304403391285653	19.442119924678558	39.198172565655916	25.055304118379873
3	19.821474588804733	24.284609868686417	28.122338995204586	27.77157654730426
4	13.48761677874485	15.312239805997251	35.180995237465	36.0191481777929
5	14.30889469371748	37.860749476514954	32.33435812679893	15.495997702968634
6	33.403277125996944	34.1370561933196	17.72836858556551	14.73129809511795
7	29.272321947760975	32.966479421478226	19.54787923455998	18.213319396200824
8	27.211355180745173	35.247883197770655	18.68572273200762	18.85503888947655
9	27.84458167082338	16.61172241984408	18.20738178932646	37.33631412000608
10-11	26.420994781288016	26.013102298173784	26.231987667601075	21.333915252937125
12-13	25.970700074930498	23.44001008410423	26.62616684990791	23.963122991057357
14-15	23.919179482007394	24.570469840963007	23.89589159682052	27.61445908020908
16-17	24.428217163833857	27.450689235279963	24.484636362331027	23.63645723855515
18-19	24.447174791394644	26.811287104854664	25.802479167483906	22.93905893626679
20-21	25.18527364327023	25.82603573587308	25.491944334909473	23.49674628594722
22-23	25.163634351428694	25.27451590884177	25.39065085653026	24.17119888319927
24-25	24.646352580340032	25.416947269736507	25.431515865936692	24.505184283986768
26-27	24.241402125436554	25.65126126211324	25.550807222463316	24.55652938998689
28-29	24.450849377480438	26.205819709424322	25.411554307726092	23.931776605369144
30-31	25.195122907821588	25.686046821093445	25.42764554025793	23.69118473082704
32-33	24.64812482073986	25.634086234677305	25.501009109829603	24.216779834753233
34-35	24.541881676422882	26.067886167329164	25.53498069947039	23.855251456777566
36-37	24.63784100227376	25.9548623876339	25.434426235248065	23.97287037484428
38-39	24.736724521790045	26.014845816551425	25.337336479262568	23.911093182395962
40-41	24.65050970909491	25.76817114849686	25.524471850594608	24.056847291813625
42-43	24.6088013138589	25.813825633555226	25.220453057208193	24.356919995377684
44-45	24.203643010210513	25.86733336134571	25.75398826281216	24.175035365631608
46-47	24.182081197103468	25.72426377765397	25.625338578061434	24.46831644718112
48-49	24.28889359712696	25.974719096205064	25.51315612069977	24.223231185968206
50-51	24.283333455901793	26.041315376703654	25.57884706502817	24.096504102366374
52-53	24.331719903320266	25.947204349047176	25.412118024354836	24.30895772327773
54-55	24.161476415024964	25.559850319049023	25.821890033666307	24.456783232259703
56-57	24.28414195261359	25.797048566182788	25.41100402048025	24.507805460723365
58-59	24.180478982577835	26.01979449164783	25.564272570815294	24.23545395495904
60-61	24.725698976052566	25.934819080208694	25.576484751794037	23.76299719194471
62-63	24.477358871137184	25.985261695255225	25.592186541386873	23.945192892220717
64-65	24.27935365151178	25.62793816653156	25.61897374931498	24.473734432641685
66-67	24.293842885689013	25.847497098608102	25.45639577436872	24.40226424133417
68-69	24.146425847551107	25.775554698010783	25.72075198427845	24.35726747015966
70-71	24.14825275836476	25.750995694285518	25.838104715060332	24.26264683228939
72-73	24.18757001291792	26.24494742662366	25.28245911805872	24.2850234423997
74-75	24.21393215813406	26.124765981941	25.380950063426027	24.280351796498913
76-77	24.069227022573436	25.661766482403493	25.657599639200402	24.611406855822672
78-79	24.025822808746977	25.768284590184603	25.801517361409015	24.404375239659405
80-81	24.260732332737945	25.984677675480324	25.39682817587723	24.357761815904503
82-83	24.423258411817955	26.059415440590687	25.45062341214187	24.066702735449486
84-85	24.364699130850077	26.202648521065587	25.164626582059093	24.268025766025247
86-87	24.082001965100215	26.333288279360772	25.301190475356762	24.28351928018225
88-89	23.968110452941662	25.99316042261669	25.42784126801815	24.610887856423503
90-91	24.442642233163888	25.738098314593955	25.523338694445	24.29592075779716
92-93	24.339530221339437	26.018946742616095	25.525688602438773	24.115834433605695
94-95	24.24650919973235	25.92840283654767	25.427143560922644	24.397944402797332
96-97	24.0751874275842	25.86642473501376	25.511755826440886	24.546632010961154
98-99	23.912199381475713	25.909326286091815	25.412889295496427	24.76558503693605
100-101	23.882338276754066	26.149208767907588	25.526601056283287	24.44185189905506
102-103	23.752779430099107	25.976533574955425	25.664901298940464	24.605785696005007
104-105	24.006456096617377	26.07468275322411	25.49853564945216	24.420325500706355
106-107	23.915232364933132	26.011106575369897	25.619099500547204	24.454561559149763
108-109	23.92270598385407	26.00386841235427	25.586949640610207	24.486475963181455
110-111	23.76418740343778	26.402618194956517	25.551452761403752	24.28174164020195
112-113	23.631827558246847	26.492669516982158	25.555510431006358	24.319992493764644
114-115	23.742368214376626	26.65642617620623	25.37690175326674	24.224303856150403
116-117	23.71995201986428	26.401573849285516	25.41039051432997	24.468083616520236
118-119	23.590740463131315	26.259543539111352	25.60987897386351	24.539837023893824
120-121	23.653240411872765	26.38343266280832	25.74516361792923	24.218163307389688
122-123	23.575229666143848	26.541248879677347	25.54895809993278	24.334563354246022
124-125	23.541069622387052	26.432695549561696	25.457054956169927	24.56917987188132
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	618.0
1	517.0
2	362.0
3	284.0
4	233.0
5	201.0
6	205.0
7	238.0
8	269.0
9	312.5
10	357.0
11	363.0
12	376.5
13	409.0
14	439.0
15	488.0
16	525.5
17	592.0
18	693.0
19	757.5
20	812.0
21	920.5
22	1112.0
23	1364.5
24	1639.0
25	2004.5
26	2691.0
27	3726.5
28	4740.5
29	6028.0
30	7849.0
31	9756.5
32	12064.0
33	14466.0
34	16670.5
35	18847.0
36	21809.0
37	24761.0
38	26973.5
39	29378.0
40	31979.5
41	34622.5
42	37015.0
43	40617.5
44	44590.5
45	48178.0
46	51641.0
47	54868.0
48	58943.5
49	62985.5
50	65820.5
51	66379.0
52	64789.0
53	63442.5
54	62510.0
55	59790.0
56	55462.5
57	50742.5
58	46258.0
59	41005.5
60	34793.5
61	29388.0
62	24448.0
63	18979.0
64	14216.5
65	10968.5
66	8459.5
67	6643.0
68	5219.5
69	4010.5
70	3143.5
71	2467.0
72	1911.0
73	1586.0
74	1281.0
75	938.5
76	712.5
77	496.0
78	315.5
79	209.0
80	162.0
81	122.5
82	98.5
83	75.0
84	53.0
85	49.5
86	44.0
87	35.0
88	27.0
89	23.0
90	22.0
91	16.0
92	11.0
93	14.0
94	14.0
95	7.5
96	8.0
97	9.0
98	6.5
99	5.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027447559057764512
2	0.011553181746252918
3	0.010222815363351068
4	0.010993027479767927
5	0.01736478226103469
6	0.0922854154034021
7	0.05713573518146898
8	0.046982939101428534
9	0.025697076974998914
10-11	0.05030885505868316
12-13	0.013163625262397264
14-15	0.027797655474317628
16-17	0.03308411136426972
18-19	0.0421516085529955
20-21	0.02975819540701509
22-23	0.037005191229664655
24-25	0.03147366784812537
26-27	0.04617771734335636
28-29	0.04428719669396952
30-31	0.025206941991824546
32-33	0.030423378598466017
34-35	0.04386708099410577
36-37	0.010152796080040442
38-39	0.02107580427649775
40-41	0.04194155070306362
42-43	0.02261622850933147
44-45	0.01666458942792845
46-47	0.023211392417471773
48-49	0.029443108632117288
50-51	0.028322800099147307
52-53	0.02597715410824141
54-55	0.023876575608922698
56-57	0.025452009483411732
58-59	0.01788992688586437
60-61	0.022511199584365534
62-63	0.02338644062574833
64-65	0.021740987467948673
66-67	0.029058002573908857
68-69	0.023036344209195214
70-71	0.04596765949342449
72-73	0.022161103167812415
74-75	0.009557632171900142
76-77	0.016699599069583765
78-79	0.025942144466586096
80-81	0.018310042585728107
82-83	0.024541758800373625
84-85	0.01214834565439322
86-87	0.0182750329440728
88-89	0.021846016392914606
90-91	0.021180833201463682
92-93	0.008577362205551409
94-95	0.012218364937703844
96-97	0.015859367669856277
98-99	0.00710695725602831
100-101	0.01064293106321481
102-103	0.003676012373807747
104-105	0.004936359473398974
106-107	0.0010502892496593562
108-109	0.014143895228745996
110-111	0.0012953567412465393
112-113	0.00224061706593996
114-115	0.013058596337431328
116-117	0.0060916776480242655
118-119	0.27878177650124847
120-121	0.0012953567412465393
122-123	0.0012603470995912274
124-125	0.31494673633118564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1428178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.99146292510191
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.11275184655781	54.84732082085315
2	9.453085997760777	12.476459482980607
3	2.949593244813552	5.839439197777336
4	1.3721809018515956	3.6220890044428993
5	0.8046909330787447	2.6551365938215823
6	0.5087096527207586	2.014229651229805
7	0.35868341201000203	1.6569030159854972
8	0.2414177849438543	1.274521024366607
9	0.1917209841001507	1.1386753392788234
>10	0.9512217000976283	10.872160704796215
>50	0.0425025981282257	1.8670503509741487
>100	0.012911798010503711	1.4324280382246266
>500	4.2331674116274584E-4	0.22508860747435638
>1k	1.0582918529068646E-4	0.07849816779434582
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.001928331062375E-5	0.0
3	0.0	0.0	0.0	7.001928331062375E-5	0.0
4	0.0	0.0	0.0	7.001928331062375E-5	0.0
5	0.0	0.0	0.0	7.001928331062375E-5	0.0
6	0.0	0.0	0.0	7.001928331062375E-5	7.001928331062375E-5
7	0.0	0.0	0.0	1.400385666212475E-4	7.001928331062375E-5
8	0.0	0.0	0.0	1.400385666212475E-4	7.001928331062375E-5
9	0.0	0.0	0.0	1.400385666212475E-4	7.001928331062375E-5
10-11	0.0	0.0	0.0	2.1005784993187123E-4	7.001928331062375E-5
12-13	0.0	0.0	0.0	2.1005784993187123E-4	1.400385666212475E-4
14-15	0.0	0.0	0.0	2.80077133242495E-4	1.400385666212475E-4
16-17	0.0	0.0	0.0	2.80077133242495E-4	1.400385666212475E-4
18-19	0.0	0.0	0.0	2.80077133242495E-4	1.400385666212475E-4
20-21	0.0	0.0	0.0	3.500964165531187E-4	1.400385666212475E-4
22-23	0.0	0.0	0.0	4.2011569986374247E-4	1.400385666212475E-4
24-25	0.0	0.0	0.0	4.2011569986374247E-4	2.1005784993187123E-4
26-27	0.0	0.0	0.0	8.752410413827968E-4	4.2011569986374247E-4
28-29	0.0	0.0	0.0	0.0023456459909058955	4.551253415190543E-4
30-31	0.0	0.0	0.0	0.007422044030926117	4.901349831743662E-4
32-33	0.0	0.0	0.0	0.021005784993187122	4.901349831743662E-4
34-35	0.0	0.0	0.0	0.03546476699683093	4.901349831743662E-4
36-37	0.0	0.0	0.0	0.057065715898158356	4.901349831743662E-4
38-39	0.0	0.0	0.0	0.0964165531187289	4.901349831743662E-4
40-41	0.0	0.0	0.0	0.14753062993548421	5.251446248296781E-4
42-43	0.0	0.0	0.0	0.19251801946255997	5.6015426648499E-4
44-45	0.0	0.0	0.0	0.2393959296390226	5.6015426648499E-4
46-47	0.0	0.0	0.0	0.29348582599647943	5.6015426648499E-4
48-49	0.0	0.0	0.0	0.3537724289269265	5.6015426648499E-4
50-51	0.0	0.0	0.0	0.42197121087147405	5.6015426648499E-4
52-53	0.0	0.0	0.0	0.4957365258392161	6.651831914509256E-4
54-55	0.0	0.0	0.0	0.5741931327887699	7.001928331062374E-4
56-57	0.0	0.0	0.0	0.6555905496373702	7.001928331062374E-4
58-59	0.0	0.0	0.0	0.7491713217820188	7.001928331062374E-4
60-61	0.0	0.0	0.0	0.8460079906006114	7.001928331062374E-4
62-63	0.0	0.0	0.0	0.9602794609635494	7.001928331062374E-4
64-65	0.0	0.0	0.0	1.0648882702296212	7.001928331062374E-4
66-67	0.0	0.0	0.0	1.1788446538176613	7.352024747615493E-4
68-69	0.0	0.0	0.0	1.3078901929591409	7.702121164168612E-4
70-71	0.0	0.0	0.0	1.4445328243398232	9.102506830381087E-4
72-73	0.0	0.0	0.0	1.5750487684308259	9.102506830381087E-4
74-75	0.0	0.0	0.0	1.7140370458024141	9.102506830381087E-4
76-77	0.0	0.0	0.0	1.8797726893986604	9.102506830381087E-4
78-79	0.0	0.0	0.0	2.05937215109041	9.102506830381087E-4
80-81	0.0	0.0	0.0	2.233685156892208	9.452603246934205E-4
82-83	0.0	0.0	0.0	2.4177308430741826	0.0011553181746252919
84-85	7.001928331062375E-5	0.0	0.0	2.6209618128832677	0.0011903278162806036
86-87	7.001928331062375E-5	0.0	0.0	2.8311597013817607	0.0012253374579359155
88-89	7.001928331062375E-5	0.0	0.0	3.0774525304268794	0.0012603470995912274
90-91	7.001928331062375E-5	0.0	0.0	3.3432807395156625	0.0012603470995912274
92-93	7.001928331062375E-5	0.0	0.0	3.5932495809345895	0.0014704049495230986
94-95	7.001928331062375E-5	0.0	0.0	3.859637944289857	0.0015404242328337224
96-97	7.001928331062375E-5	0.0	0.0	4.156799782660144	0.001610443516144346
98-99	7.001928331062375E-5	0.0	0.0	4.495413036750321	0.0016804627994549699
100-101	7.001928331062375E-5	0.0	0.0	4.8521262755762935	0.0016804627994549699
102-103	7.001928331062375E-5	0.0	0.0	5.221442985398179	0.0017504820827655937
104-105	7.001928331062375E-5	0.0	0.0	5.605148657940397	0.0017504820827655937
106-107	7.001928331062375E-5	0.0	0.0	6.007024334501722	0.0017854917244209056
108-109	7.001928331062375E-5	0.0	0.0	6.429310632148093	0.0018205013660762175
110-111	7.001928331062375E-5	0.0	0.0	6.892733258739457	0.0018555110077315292
112-113	7.001928331062375E-5	0.0	0.0	7.361372321937461	0.001890520649386841
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8800	0.0	35.712666	1
TACATGG	8970	0.0	34.56528	2
GTATCAA	3590	0.0	33.988373	1
CATGGGG	7395	0.0	33.557743	4
TAACGCA	125	1.2765158E-6	33.325977	4
ACATGGG	9465	0.0	32.506096	3
GGTATCA	2570	0.0	31.26595	1
ATGGGGG	4825	0.0	30.339067	5
TCAACGC	4285	0.0	27.35963	4
TATCAAC	4445	0.0	27.178104	2
CAACGCA	4320	0.0	27.136066	5
ATCAACG	4465	0.0	26.523232	3
AACGCAG	4480	0.0	26.298824	6
GTATAGA	490	0.0	25.509045	1
TGGGGGG	4000	0.0	23.504246	6
ATGGGGA	2540	0.0	22.490646	5
TATAACG	160	2.6085236E-4	22.316504	2
ACGCAGA	5270	0.0	22.126797	7
CGCAGAG	5620	0.0	20.853195	8
GTCTTAT	415	2.910383E-11	20.07941	1
>>END_MODULE