FastQCFastQC Report
Mon 15 Aug 2016
SRR2921591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1428178
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32770.22945319140891401No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.18085980879134114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.13338673470673823No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34450.043.796531
GTATCAA52550.042.6700941
TCAACGC63750.034.7895854
CAACGCA65300.033.845185
AACGCAG67250.033.2356036
TATCAAC67300.033.04772
ATCAACG67500.033.0369573
GTACATG85850.032.440311
CATGGGG68350.032.2737734
ATGGGGG43800.031.6896825
TACATGG88150.031.1835882
ACATGGG89150.030.6320693
ACGCAGA77000.028.9487767
CGCAGAG80400.027.7236048
TGGGGGG34400.026.5092586
GCAGAGT86450.025.3697959
GAGTACT37350.024.7116712-13
GTACTTT39150.023.41519514-15
GTATACG1300.002025338622.936921
AGTACTT40250.022.19148412-13
Produced by FastQC (version 0.11.5)