##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1428178
Sequences flagged as poor quality	0
Sequence length	125
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.765044693308536	33.0	31.0	34.0	30.0	34.0
2	31.933976717187914	33.0	31.0	34.0	30.0	34.0
3	31.98802320158972	34.0	31.0	34.0	30.0	34.0
4	35.382557356295926	37.0	35.0	37.0	33.0	37.0
5	35.23633118560851	37.0	35.0	37.0	33.0	37.0
6	35.26283838569142	37.0	35.0	37.0	33.0	37.0
7	35.15084604300024	37.0	35.0	37.0	33.0	37.0
8	35.18723226376544	37.0	36.0	37.0	33.0	37.0
9	36.90371648351956	39.0	38.0	39.0	34.0	39.0
10-11	36.848613758229014	39.0	38.0	39.0	33.5	39.0
12-13	36.77600551191799	39.0	38.0	39.0	33.0	39.0
14-15	38.196953040867456	41.0	39.0	41.0	34.0	41.0
16-17	38.20669622414013	41.0	39.0	41.0	34.0	41.0
18-19	38.22829787323429	41.0	39.0	41.0	34.0	41.0
20-21	38.17399616854482	41.0	39.0	41.0	34.0	41.0
22-23	38.18039207997882	41.0	39.0	41.0	34.0	41.0
24-25	38.136503993199725	41.0	39.0	41.0	34.0	41.0
26-27	38.05571784469443	41.0	39.0	41.0	33.5	41.0
28-29	37.98484047506683	41.0	38.5	41.0	33.0	41.0
30-31	37.855938825552556	41.0	38.0	41.0	33.0	41.0
32-33	37.71566604442864	41.0	38.0	41.0	33.0	41.0
34-35	37.579364757054094	40.5	38.0	41.0	33.0	41.0
36-37	37.50388326945241	40.0	38.0	41.0	32.5	41.0
38-39	37.42064504564557	40.0	38.0	41.0	32.0	41.0
40-41	37.29089091135699	40.0	38.0	41.0	32.0	41.0
42-43	37.12273820210086	40.0	37.5	41.0	31.5	41.0
44-45	36.947608771455656	40.0	37.0	41.0	31.0	41.0
46-47	36.70667346787305	40.0	37.0	41.0	30.0	41.0
48-49	36.49034609131355	40.0	36.5	41.0	30.0	41.0
50-51	36.239271995507565	40.0	36.0	41.0	29.0	41.0
52-53	36.11232108322632	40.0	36.0	41.0	29.0	41.0
54-55	35.98944529323376	40.0	35.0	41.0	29.0	41.0
56-57	35.798998794267945	39.0	35.0	41.0	28.0	41.0
58-59	35.61001604841974	39.0	35.0	41.0	28.0	41.0
60-61	35.361054434391235	39.0	35.0	41.0	28.0	41.0
62-63	35.005153769348084	38.0	35.0	41.0	28.0	41.0
64-65	34.72147834513625	37.0	35.0	40.0	28.0	41.0
66-67	34.41729322255349	37.0	35.0	39.5	28.0	41.0
68-69	34.049120277724484	36.0	35.0	39.0	26.5	41.0
70-71	33.67479263789248	36.0	34.5	39.0	26.0	41.0
72-73	33.35171806315459	35.0	34.0	37.5	26.0	40.0
74-75	33.02563440971644	35.0	34.0	37.0	26.0	39.0
76-77	32.69484896140397	35.0	34.0	37.0	26.0	39.0
78-79	32.43090287065058	35.0	34.0	36.0	26.0	38.5
80-81	32.17735149260106	35.0	34.0	36.0	25.0	37.0
82-83	31.95607865406133	35.0	34.0	35.5	25.0	37.0
84-85	31.740876487384625	35.0	34.0	35.0	24.5	36.5
86-87	31.60438824852364	35.0	34.0	35.0	24.0	36.0
88-89	31.506464530331655	35.0	33.0	35.0	24.0	36.0
90-91	31.398200714476765	35.0	33.0	35.0	24.0	36.0
92-93	31.311595263335523	35.0	33.0	35.0	23.0	35.0
94-95	31.240901344230203	35.0	33.0	35.0	23.0	35.0
96-97	31.17694468056503	35.0	33.0	35.0	23.0	35.0
98-99	31.10111099596829	35.0	33.0	35.0	21.0	35.0
100-101	31.035737142008912	35.0	33.0	35.0	20.0	35.0
102-103	30.353702409643617	34.0	31.5	35.0	19.5	35.0
104-105	30.866065014304937	35.0	33.0	35.0	19.5	35.0
106-107	30.964416900414374	35.0	33.0	35.0	20.0	35.0
108-109	30.950610498131184	35.0	33.0	35.0	19.5	35.0
110-111	30.887398139447605	35.0	33.0	35.0	19.0	35.0
112-113	30.85440785392297	35.0	33.0	35.0	19.0	35.0
114-115	30.734223605180865	35.0	33.0	35.0	17.5	35.0
116-117	30.91369178071641	35.0	33.0	35.0	18.5	35.0
118-119	30.89724950251299	35.0	33.0	35.0	16.5	35.0
120-121	30.825598419804813	35.0	33.0	35.0	10.0	35.0
122-123	30.697553456221844	35.0	33.0	35.0	2.0	35.0
124-125	30.062130560756433	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22325.0
3	6284.0
4	3401.0
5	2587.0
6	2702.0
7	3104.0
8	3608.0
9	3786.0
10	4179.0
11	4576.0
12	5304.0
13	6995.0
14	9938.0
15	9162.0
16	5057.0
17	4337.0
18	3891.0
19	3419.0
20	3203.0
21	3168.0
22	3440.0
23	4092.0
24	5591.0
25	7287.0
26	8804.0
27	10111.0
28	11982.0
29	14294.0
30	17275.0
31	21669.0
32	28230.0
33	38456.0
34	61029.0
35	107739.0
36	245185.0
37	530685.0
38	200898.0
39	385.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.80225601718977	18.090929847194264	11.547765624102658	26.559048511513307
2	15.708880134775383	19.824052588166264	38.20498349617758	26.262083780880772
3	18.687053698067878	24.426684784321694	28.63091464973506	28.255346867875364
4	13.269574438836628	15.270292638176223	34.4466358252419	37.013497097745244
5	14.258199890182544	37.794083864678	32.39425867613935	15.5534575690001
6	33.76725564631513	34.26395761252342	16.88723625188654	15.081550489274909
7	29.840814150538268	32.14471109691331	19.38707528314457	18.627399469403855
8	28.11776960380844	34.97391015623085	18.3854768287583	18.522843411202416
9	28.354438181903507	15.586676033628608	18.338839808841133	37.72004597562675
10-11	26.908208617193118	25.354793349142017	26.123391120628863	21.613606913035998
12-13	26.76550938761813	22.642026278114777	26.827756061694615	23.764708272572477
14-15	24.23632695711633	23.774916165246154	24.577445867777715	27.4113110098598
16-17	24.210125165239553	26.904291709895737	25.08891586741045	23.796667257454256
18-19	24.133619675217698	25.895063544363378	27.18234122314494	22.78897555727398
20-21	24.913759685511312	24.989503386755462	26.988884970775818	23.107851956957408
22-23	24.624598340355302	24.479794305448287	26.991738187582843	23.903869166613568
24-25	24.180339491487505	24.661956233656245	27.004428591285034	24.153275683571216
26-27	23.792261736375835	24.966984659595383	27.11343692844599	24.127316675582794
28-29	23.968320323112817	25.51718777571444	26.906333414559686	23.608158486613057
30-31	24.67169721746565	25.04806074752449	26.88650031281936	23.393741722190498
32-33	24.325890435947468	24.91848152428783	26.993198069676104	23.76242997008859
34-35	24.21785065078747	25.252640541804727	26.9674872349305	23.562021572477306
36-37	24.251767143541876	25.191454409289666	26.978222181297074	23.57855626587138
38-39	24.379911535469486	25.303103400709876	26.721308720485276	23.595676343335366
40-41	24.3765956225151	25.010608307670328	26.900462564227528	23.71233350558704
42-43	24.265878496777404	25.055938985455338	26.56967891670054	24.10850360106672
44-45	23.947027519081296	25.15877739654086	26.89668090469855	23.997514179679293
46-47	23.99533928316971	25.125927233017986	26.572002858330247	24.30673062548206
48-49	24.08007705075694	25.489873434118763	26.316992723800976	24.113056791323316
50-51	24.173549668243524	25.61327333988724	26.224656130002604	23.98852086186663
52-53	24.282480871466408	25.55074947441389	26.030805210173174	24.135964443946527
54-55	23.98245921153981	25.183040403333102	26.440655416287374	24.393844968839716
56-57	24.162493488161058	25.36536317856163	26.087862212288748	24.384281120988568
58-59	24.050199941323473	25.470199955327416	26.3144627841663	24.165137319182815
60-61	24.46592716864028	25.351777371976564	26.389394584420966	23.79290087496219
62-63	24.324245788384875	25.506428360618376	26.31939550338599	23.84993034761076
64-65	24.04659926605736	25.09489370015061	26.4173138203458	24.441193213446233
66-67	24.064390924995475	25.36897271623788	26.22166686914725	24.344969489619398
68-69	23.975463713891305	25.298765098424873	26.439105134856096	24.286666052827727
70-71	23.986032543585285	25.2437197910343	26.503542628119625	24.266705037260788
72-73	24.06131150835399	25.720311493999752	26.009531915251173	24.208845082395086
74-75	24.035501715566138	25.60776556263567	26.002485820320704	24.354246901477488
76-77	23.987504148712254	25.090956577511946	26.186461684104096	24.735077589671704
78-79	23.77838850280953	25.295884607978714	26.38832776095367	24.53739912825809
80-81	24.005025984156035	25.530956225344458	25.96868177496082	24.49533601553868
82-83	24.172494972298626	25.55990947336579	25.974729848580758	24.292865705754828
84-85	24.035570493295523	25.76823162833036	25.713650526905436	24.482547351468682
86-87	23.829907756596167	25.775708630156746	25.90871025880527	24.485673354441815
88-89	23.62716808539093	25.504827536951368	26.055675795656093	24.81232858200161
90-91	24.069937076796556	25.238215772007454	26.103694274384097	24.588152876811893
92-93	24.000649778949125	25.557948659060703	26.002781165933097	24.438620396057072
94-95	23.90025690956489	25.39980632001731	25.941038939759643	24.758897830658157
96-97	23.726578254537305	25.389137071476586	25.96515796549406	24.919126708492044
98-99	23.631877286360417	25.41475265033132	25.874221600292124	25.079148463016143
100-101	23.551371047586507	25.52265894027215	26.036075335147302	24.889894676994047
102-103	23.496106878781088	25.34064810665472	26.079640936589737	25.08360407797446
104-105	23.755092152378765	25.469899410297597	25.918828045243657	24.85618039207998
106-107	23.725563579418562	25.339376496013603	25.977255516760778	24.957804407807053
108-109	23.744920830424533	25.260286653171228	25.954687820566235	25.040104695838007
110-111	23.53611508373434	25.686302998051712	25.970336320240307	24.80724559797364
112-113	23.460578521742267	25.676239143045898	25.92015191420936	24.943030421002476
114-115	23.451558559227212	25.810333165753846	25.78554633946189	24.952561935557053
116-117	23.370222419835383	25.588083926464567	25.859693100538806	25.18200055316125
118-119	23.380314925343875	25.417988891907562	26.00847391166742	25.19322227108115
120-121	23.295584642563803	25.519438370063206	26.159821696779495	25.0251552905935
122-123	23.17385784936012	25.64626817284606	25.96853576032394	25.21133821746988
124-125	23.01694112138462	25.617689589646215	25.906023003588135	25.459346285381034
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	22.5
2	37.5
3	82.0
4	91.0
5	91.5
6	191.5
7	546.0
8	934.5
9	1045.5
10	892.5
11	732.5
12	749.0
13	836.0
14	924.5
15	973.0
16	1031.0
17	1103.0
18	1202.5
19	1270.5
20	1321.5
21	1410.5
22	1521.5
23	1734.0
24	1976.0
25	2368.0
26	3020.5
27	3846.5
28	4836.0
29	6124.5
30	7852.5
31	9724.5
32	11960.0
33	14134.5
34	16284.0
35	18754.5
36	21508.5
37	24239.5
38	26347.0
39	28722.0
40	31477.5
41	33811.5
42	35915.5
43	39580.5
44	44105.0
45	48427.5
46	51833.5
47	54403.5
48	58451.5
49	62678.0
50	66324.5
51	67635.0
52	66187.0
53	64486.0
54	63064.0
55	60421.0
56	55881.5
57	50735.5
58	45960.5
59	41018.0
60	35121.5
61	29612.5
62	24362.0
63	18616.5
64	13678.0
65	10331.0
66	7844.5
67	6108.0
68	4773.0
69	3625.0
70	2826.0
71	2181.5
72	1649.5
73	1333.5
74	1062.0
75	789.0
76	574.5
77	352.0
78	189.0
79	114.5
80	76.0
81	45.0
82	22.0
83	11.0
84	6.5
85	5.0
86	4.0
87	2.5
88	2.5
89	2.0
90	1.5
91	2.0
92	1.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024646787725339556
2	0.00168046279945497
3	0.03311912100592503
4	0.16734608711239074
5	0.024786826291960808
6	0.0674985891114413
7	0.050904018966823464
8	0.042781782102791106
9	0.032558966739440046
10-11	0.06326242247114855
12-13	0.05549028202366932
14-15	0.057170744823124287
16-17	0.049363594733989734
18-19	0.035989911621660604
20-21	0.02356148883402489
22-23	0.0028707906157355734
24-25	0.0050063787567095975
26-27	0.003781041298773682
28-29	0.004901349831743663
30-31	0.0026257231241483903
32-33	0.008927458622104527
34-35	0.004341195565258672
36-37	0.006756860839475191
38-39	0.0024506749158718314
40-41	0.003641002732152435
42-43	0.0041661473569821125
44-45	0.005461504098228652
46-47	0.0050063787567095975
48-49	0.0019955495743527764
50-51	0.0015404242328337222
52-53	7.352024747615493E-4
54-55	0.005461504098228652
56-57	7.001928331062375E-4
58-59	6.301735497956137E-4
60-61	1.400385666212475E-4
62-63	0.0010152796080040445
64-65	7.001928331062375E-5
66-67	0.004026108790360866
68-69	5.251446248296781E-4
70-71	7.001928331062375E-5
72-73	0.0019955495743527764
74-75	0.005461504098228652
76-77	0.00168046279945497
78-79	2.1005784993187126E-4
80-81	0.0
82-83	0.0063017354979561365
84-85	0.0019605399326974647
86-87	0.0
88-89	0.0011903278162806038
90-91	5.951639081403019E-4
92-93	0.0
94-95	0.003430944882220564
96-97	0.0012603470995912274
98-99	5.251446248296781E-4
100-101	0.0
102-103	0.0012603470995912274
104-105	0.0
106-107	5.251446248296781E-4
108-109	0.0028707906157355734
110-111	3.5009641655311874E-5
112-113	2.450674915871831E-4
114-115	0.0
116-117	0.001610443516144346
118-119	0.0021005784993187123
120-121	0.003430944882220564
122-123	0.0011903278162806038
124-125	0.10719952274856495
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1428178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.28499383696497
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.67494295776781	50.248383249220694
2	10.75651736967974	13.399392361554185
3	3.3731366424932263	6.3028738496679395
4	1.6422219725051912	4.0914314174565725
5	0.892577042686776	2.7797077801381134
6	0.5734601843917092	2.1430778430349444
7	0.40822918165183575	1.7798586444277689
8	0.3032540886536951	1.5110543234263862
9	0.20248339234437046	1.1350509159831186
>10	1.1063416700041746	11.930822287794616
>50	0.049343389716652905	2.089543421434005
>100	0.01658932116642743	1.746336748867861
>500	5.64241836472855E-4	0.29668395375196976
>1k	3.3854510188371305E-4	0.5457832032418966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3277	0.22945319140891401	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2583	0.18085980879134114	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1905	0.13338673470673823	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.400385666212475E-4
7	0.0	0.0	0.0	0.0	1.400385666212475E-4
8	0.0	0.0	0.0	0.0	1.400385666212475E-4
9	0.0	0.0	0.0	0.0	1.400385666212475E-4
10-11	0.0	0.0	0.0	0.0	1.400385666212475E-4
12-13	0.0	0.0	0.0	7.001928331062375E-5	2.1005784993187123E-4
14-15	0.0	0.0	0.0	1.400385666212475E-4	2.4506749158718314E-4
16-17	0.0	0.0	0.0	1.400385666212475E-4	2.80077133242495E-4
18-19	0.0	0.0	0.0	1.400385666212475E-4	2.80077133242495E-4
20-21	0.0	0.0	0.0	1.400385666212475E-4	2.80077133242495E-4
22-23	0.0	0.0	0.0	2.1005784993187123E-4	4.2011569986374247E-4
24-25	0.0	0.0	0.0	2.1005784993187123E-4	4.901349831743662E-4
26-27	0.0	0.0	0.0	6.651831914509256E-4	4.901349831743662E-4
28-29	0.0	0.0	0.0	0.0022056074242846483	4.901349831743662E-4
30-31	0.0	0.0	0.0	0.0073870343892708055	4.901349831743662E-4
32-33	0.0	0.0	0.0	0.0209357657098765	5.251446248296781E-4
34-35	0.0	0.0	0.0	0.03542975735517562	5.6015426648499E-4
36-37	0.0	0.0	0.0	0.05745082195636679	5.951639081403018E-4
38-39	0.0	0.0	0.0	0.09652158204369483	6.301735497956137E-4
40-41	0.0	0.0	0.0	0.14840587097686703	6.301735497956137E-4
42-43	0.0	0.0	0.0	0.1934282701455981	6.301735497956137E-4
44-45	0.0	0.0	0.0	0.24065627673861384	6.301735497956137E-4
46-47	0.0	0.0	0.0	0.2944310863211729	6.301735497956137E-4
48-49	0.0	0.0	0.0	0.35405250606016897	7.001928331062374E-4
50-51	0.0	0.0	0.0	0.4218311723048528	7.001928331062374E-4
52-53	0.0	0.0	0.0	0.49472124623121205	7.352024747615493E-4
54-55	0.0	0.0	0.0	0.5736679881639404	8.402313997274849E-4
56-57	0.0	0.0	0.0	0.6553104725041277	8.402313997274849E-4
58-59	0.0	0.0	0.0	0.7488212253654656	8.402313997274849E-4
60-61	0.0	0.0	0.0	0.8468832316419942	8.402313997274849E-4
62-63	0.0	0.0	0.0	0.9625900973127999	8.402313997274849E-4
64-65	0.0	0.0	0.0	1.0670238583705953	8.402313997274849E-4
66-67	0.0	0.0	0.0	1.1814703769418098	8.752410413827968E-4
68-69	0.0	0.0	0.0	1.311601214974604	9.102506830381087E-4
70-71	0.0	0.0	0.0	1.4491891066799796	9.102506830381087E-4
72-73	0.0	0.0	0.0	1.5796350314876717	0.0012253374579359155
74-75	0.0	0.0	0.0	1.7183782413676725	0.0012603470995912274
76-77	0.0	0.0	0.0	1.8860044056133058	0.0012603470995912274
78-79	0.0	0.0	0.0	2.0677744650876853	0.0012603470995912274
80-81	0.0	0.0	0.0	2.2431027504974868	0.0013303663829018512
82-83	0.0	0.0	0.0	2.4268683595462193	0.0014003856662124748
84-85	0.0	0.0	0.0	2.6302743775635813	0.0014003856662124748
86-87	0.0	0.0	0.0	2.844183288077536	0.0015054145911784105
88-89	0.0	7.001928331062375E-5	0.0	3.0942221487797736	0.0015404242328337224
90-91	0.0	7.001928331062375E-5	0.0	3.3629911677676034	0.0015404242328337224
92-93	0.0	7.001928331062375E-5	0.0	3.61520062625247	0.0015404242328337224
94-95	0.0	7.001928331062375E-5	0.0	3.883164423482227	0.0015404242328337224
96-97	0.0	7.001928331062375E-5	0.0	4.183337091034871	0.001715472441110282
98-99	0.0	7.001928331062375E-5	0.0	4.523000634374707	0.0018205013660762175
100-101	0.0	7.001928331062375E-5	0.0	4.88090420101696	0.0018205013660762175
102-103	0.0	1.400385666212475E-4	0.0	5.250150891555535	0.0018205013660762175
104-105	0.0	1.400385666212475E-4	0.0	5.632176101298297	0.0018205013660762175
106-107	0.0	1.400385666212475E-4	0.0	6.034541912842797	0.0018555110077315292
108-109	0.0	1.400385666212475E-4	0.0	6.461799579604223	0.001925530291042153
110-111	0.0	1.400385666212475E-4	0.0	6.929878488535742	0.0019605399326974647
112-113	0.0	1.400385666212475E-4	0.0	7.402788728015695	0.0019605399326974647
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3445	0.0	43.79653	1
GTATCAA	5255	0.0	42.670094	1
TCAACGC	6375	0.0	34.789585	4
CAACGCA	6530	0.0	33.84518	5
AACGCAG	6725	0.0	33.235603	6
TATCAAC	6730	0.0	33.0477	2
ATCAACG	6750	0.0	33.036957	3
GTACATG	8585	0.0	32.44031	1
CATGGGG	6835	0.0	32.273773	4
ATGGGGG	4380	0.0	31.689682	5
TACATGG	8815	0.0	31.183588	2
ACATGGG	8915	0.0	30.632069	3
ACGCAGA	7700	0.0	28.948776	7
CGCAGAG	8040	0.0	27.723604	8
TGGGGGG	3440	0.0	26.509258	6
GCAGAGT	8645	0.0	25.369795	9
GAGTACT	3735	0.0	24.71167	12-13
GTACTTT	3915	0.0	23.415195	14-15
GTATACG	130	0.0020253386	22.93692	1
AGTACTT	4025	0.0	22.191484	12-13
>>END_MODULE