FastQCFastQC Report
Mon 15 Aug 2016
SRR2921590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943255
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26300.051.3803631
GTATCAA39450.044.2127651
GTACATG112050.035.116941
TCAACGC49950.034.9106984
CAACGCA51500.033.7435535
TACATGG116650.033.469212
ACATGGG117900.032.4581343
TATCAAC53550.032.11922
ATCAACG53500.032.037983
AACGCAG55800.031.245076
CATGGGG102600.030.9176334
ATGGGGG60000.028.0705175
ACGCAGA65550.026.5034437
CGCAGAG70550.024.62518
ATGGGGA37400.021.9595135
GCAGAGT78600.021.6483339
GTGGTAT15200.021.5400241
CAGAGTA76200.020.14270410-11
AGAGTAC71300.019.77478610-11
GAGTACA80300.019.4228441
Produced by FastQC (version 0.11.5)