FastQCFastQC Report
Mon 15 Aug 2016
SRR2921590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943255
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28390.14609508273489583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22900.1178435151331143No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31950.058.623851
GTATCAA56900.051.36881
TCAACGC73550.039.239324
CAACGCA76050.037.9161455
TATCAAC77150.037.492382
ATCAACG77050.037.4626663
AACGCAG79550.036.333126
ACGCAGA94000.030.6210737
GTACATG115200.030.2395941
CGCAGAG98550.029.0841278
TACATGG118850.028.954352
ACATGGG120250.028.318943
CATGGGG102550.025.8170174
GCAGAGT110100.025.8165769
TAGGTCG1701.38547275E-524.531675
ATGGGGG57500.023.9343785
AGAGTAC98850.023.44987310-11
GAGTACT40450.022.81482112-13
CAGAGTA103550.022.64447210-11
GTACTTT41650.022.07919314-15
Produced by FastQC (version 0.11.5)