##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1943255
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7141553733298	33.0	31.0	34.0	30.0	34.0
2	31.885347522584528	33.0	31.0	34.0	30.0	34.0
3	31.9397675549529	34.0	31.0	34.0	30.0	34.0
4	35.33481452511379	37.0	35.0	37.0	33.0	37.0
5	35.1737924255952	37.0	35.0	37.0	33.0	37.0
6	35.1927039940718	37.0	35.0	37.0	33.0	37.0
7	35.084920404167235	37.0	35.0	37.0	33.0	37.0
8	35.119928676370314	37.0	36.0	37.0	33.0	37.0
9	36.84067299453751	39.0	38.0	39.0	34.0	39.0
10-11	36.786541910351445	39.0	38.0	39.0	33.0	39.0
12-13	36.71643428165629	39.0	38.0	39.0	33.0	39.0
14-15	38.140045953824895	41.0	39.0	41.0	33.0	41.0
16-17	38.144253842136	41.0	39.0	41.0	33.0	41.0
18-19	38.15445888470633	41.0	39.0	41.0	33.5	41.0
20-21	38.09265896652781	41.0	39.0	41.0	33.5	41.0
22-23	38.09557366377547	41.0	39.0	41.0	34.0	41.0
24-25	38.05047536221443	41.0	39.0	41.0	33.0	41.0
26-27	37.97102979279611	41.0	39.0	41.0	33.0	41.0
28-29	37.898575585808345	41.0	39.0	41.0	33.0	41.0
30-31	37.7843373617976	41.0	38.0	41.0	33.0	41.0
32-33	37.66238990765494	41.0	38.0	41.0	33.0	41.0
34-35	37.52056343609048	41.0	38.0	41.0	32.0	41.0
36-37	37.44003488991409	40.0	38.0	41.0	32.0	41.0
38-39	37.347194784009304	40.0	38.0	41.0	32.0	41.0
40-41	37.22224489323326	40.0	38.0	41.0	32.0	41.0
42-43	37.05803793120306	40.0	37.0	41.0	31.0	41.0
44-45	36.89054370116119	40.0	37.0	41.0	31.0	41.0
46-47	36.675909234763324	40.0	37.0	41.0	30.0	41.0
48-49	36.476065673316164	40.0	36.0	41.0	30.0	41.0
50-51	36.22072347684684	40.0	36.0	41.0	29.5	41.0
52-53	36.08143167005874	40.0	35.0	41.0	29.0	41.0
54-55	35.94303732654747	39.5	35.0	41.0	29.0	41.0
56-57	35.73336206519474	39.0	35.0	41.0	29.0	41.0
58-59	35.528722941662316	39.0	35.0	41.0	28.5	41.0
60-61	35.26828311261261	38.5	35.0	41.0	28.0	41.0
62-63	34.91388649456711	37.5	35.0	40.5	28.0	41.0
64-65	34.62586768077273	37.0	35.0	40.0	28.0	41.0
66-67	34.32077982560189	36.5	35.0	39.0	28.0	41.0
68-69	33.9602785017921	36.0	35.0	39.0	27.0	41.0
70-71	33.59329166784596	35.0	34.0	38.5	26.0	40.5
72-73	33.28662321723088	35.0	34.0	37.0	26.0	39.5
74-75	32.977661192174985	35.0	34.0	37.0	26.0	39.0
76-77	32.66936120066589	35.0	34.0	36.5	26.0	39.0
78-79	32.421214405726474	35.0	34.0	36.0	26.0	37.5
80-81	32.18570285423169	35.0	34.0	35.5	26.0	37.0
82-83	31.974715876197408	35.0	34.0	35.0	25.0	37.0
84-85	31.774207965501184	35.0	34.0	35.0	24.5	36.0
86-87	31.656423886726138	35.0	34.0	35.0	25.0	36.0
88-89	31.56549449248812	35.0	33.0	35.0	24.5	36.0
90-91	31.469754355450007	35.0	33.0	35.0	24.0	35.5
92-93	31.395656514456412	35.0	33.0	35.0	24.0	35.0
94-95	31.33317217760922	35.0	33.0	35.0	24.0	35.0
96-97	31.268531407355184	35.0	33.0	35.0	23.5	35.0
98-99	31.193570324018207	35.0	33.0	35.0	23.0	35.0
100-101	31.12301036148112	35.0	33.0	35.0	22.5	35.0
102-103	30.43413242214738	34.0	31.5	35.0	20.0	35.0
104-105	30.95232792402438	35.0	33.0	35.0	20.0	35.0
106-107	31.054646199289337	35.0	33.0	35.0	20.0	35.0
108-109	31.038695642105644	35.0	33.0	35.0	20.0	35.0
110-111	30.971153039616517	35.0	33.0	35.0	20.0	35.0
112-113	30.935671849551397	35.0	33.0	35.0	19.5	35.0
114-115	30.817803633594153	35.0	33.0	35.0	18.5	35.0
116-117	31.009073693365	35.0	33.0	35.0	19.0	35.0
118-119	30.992701163769034	35.0	33.0	35.0	18.5	35.0
120-121	30.911245050186412	35.0	33.0	35.0	17.5	35.0
122-123	30.779521730292732	35.0	33.0	35.0	4.5	35.0
124-125	30.141416334963758	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33918.0
3	9336.0
4	4935.0
5	3961.0
6	4058.0
7	4435.0
8	4832.0
9	5263.0
10	5510.0
11	5919.0
12	6542.0
13	8101.0
14	10900.0
15	9905.0
16	5655.0
17	4874.0
18	4479.0
19	4190.0
20	3913.0
21	4180.0
22	4720.0
23	5571.0
24	7404.0
25	9843.0
26	11590.0
27	13578.0
28	16383.0
29	19904.0
30	23868.0
31	30446.0
32	39461.0
33	54088.0
34	83659.0
35	154657.0
36	360231.0
37	716886.0
38	245750.0
39	310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95933306808139	17.73248706639414	11.344770736362388	26.963409129162088
2	16.29966987528336	19.87988915219959	36.92482265123855	26.89561832127851
3	19.018113101485774	23.839419507707863	27.95822358074916	29.184243810057207
4	13.833834195021625	15.567193645328068	33.61208047464162	36.986891685008686
5	15.248227132883835	36.78082230562031	32.09495577676467	15.875994784731184
6	34.34199244548467	33.43236082466263	16.77251742507924	15.453129304773455
7	30.483002843070928	31.19299828138885	19.348173731095535	18.975825144444684
8	28.30667074405047	33.30944563024924	18.65392793121165	19.72995569448863
9	28.218910963565285	15.285972702535473	18.34635884521656	38.14875748868268
10-11	27.005429515679374	24.69886373584047	25.976349886558964	22.319356861921197
12-13	27.30837909895363	22.33034453711418	26.292516201184053	24.06876016274814
14-15	24.917550886743108	23.50022424099187	23.907462359180006	27.674762513085017
16-17	24.810873776410546	26.353565061814997	24.47287026251263	24.362690899261825
18-19	24.776923546171105	25.357268520294056	26.35201692207388	23.513791011460956
20-21	25.747578802324593	24.281747601450185	26.125978200920997	23.844695395304225
22-23	25.57528338002192	23.94098995236967	25.923421458840068	24.560305208768344
24-25	24.97847568202368	24.553856082208743	25.890936936964753	24.57673129880282
26-27	24.638885665374886	24.700202476705144	26.154103067307226	24.506808790612737
28-29	24.646111686490453	24.87949412362579	26.09020089673406	24.384193293149696
30-31	25.086834658935793	24.476785639391014	25.961420941542695	24.474958760130498
32-33	24.84311549329553	24.499329540194008	25.89449322322233	24.76306174328813
34-35	24.932886674521534	24.76908204306523	25.983747678083503	24.314283604329734
36-37	24.997195246844008	24.71479005522019	25.924281957460387	24.36373274047542
38-39	24.939931834403815	24.774895546490367	25.981559369064268	24.303613250041558
40-41	24.959300077115643	24.51219916986824	25.9964372933001	24.53206345971602
42-43	24.854503583053507	24.474352541587866	25.85930243030092	24.811841445057702
44-45	24.793202567692436	24.678157142632912	25.71783739303104	24.810802896643615
46-47	24.852115921458882	24.74028779403451	25.582652590972284	24.824943693534323
48-49	24.804067905114806	24.993162092752126	25.486057487823636	24.71671251430943
50-51	24.85145804236469	24.95759619847861	25.50665852897919	24.68428723017751
52-53	24.991187384760583	24.81622159314587	25.344026487762427	24.848564534331118
54-55	24.745047030534145	24.724899358130667	25.47882886186236	25.051224749472826
56-57	24.801967230999377	24.955628206309157	25.211772715553778	25.03063184713769
58-59	24.740060280799224	24.94281454828768	25.464443642804618	24.852681528108484
60-61	24.957455359646943	24.799704413166992	25.632920674765458	24.609919552420607
62-63	24.796370380510016	24.864401302159184	25.579240585539363	24.759987731791437
64-65	24.752258452956507	24.643008766219566	25.484097557963313	25.12063522286061
66-67	24.74595974878499	24.977974429753868	25.316977803577203	24.95908801788394
68-69	24.71275532324112	24.949035056008046	25.365657231001197	24.972552389749634
70-71	24.55781665298687	24.787328477219923	25.59334724470026	25.06150762509295
72-73	24.716165519938656	25.029667405993177	25.324617514319094	24.929549559749077
74-75	24.57761466435737	25.085241286038908	25.293664625232964	25.043479424370762
76-77	24.617850390434835	24.757772931292322	25.26288867229513	25.361488005977712
78-79	24.52994327285302	24.902463631149782	25.35906994958702	25.20852314641018
80-81	24.598367172604725	25.101903764559978	25.264749093659866	25.03497996917543
82-83	24.620364249614866	24.985622538301943	25.34238944021433	25.051623771868858
84-85	24.558431403223885	25.01229277570747	25.117890999681197	25.31138482138745
86-87	24.295884997079643	25.23608584565587	25.193168163725293	25.27486099353919
88-89	24.341419007827685	25.00268882083499	25.222425949359597	25.433466221977724
90-91	24.373629704418835	24.93002020594991	25.303134276552118	25.393215813079138
92-93	24.3285621290052	25.036523770683722	25.35462407146772	25.28029002884336
94-95	24.3255851412977	24.8345949651204	25.34797905191195	25.491840841669948
96-97	24.18216600989848	24.90377477675671	25.35282153016052	25.56123768318429
98-99	24.18378752230806	25.01301946641801	25.17751244825663	25.625680563017305
100-101	24.080318846471513	25.060787184389078	25.35107332799864	25.507820641140768
102-103	24.03831037289137	24.88784801208913	25.513120270929868	25.560721344089632
104-105	24.039639676727965	24.99329732845149	25.32853382597755	25.638529168843
106-107	24.060101623987133	24.876263481336856	25.27168515295573	25.791949741720284
108-109	24.0363575545492	24.88534376286538	25.345023672293127	25.7332750102923
110-111	23.879739859019974	25.248397790510147	25.19511088102739	25.676751469442493
112-113	23.81799637410993	25.25942404645421	25.24061532064632	25.68196425878954
114-115	23.70926615395303	25.31631206403689	25.235236754826307	25.73918502718377
116-117	23.627830934135577	25.22446003255423	25.249341427806133	25.898367605504063
118-119	23.701823840692214	25.164225941960893	25.18264909502693	25.951301122319958
120-121	23.568257624470558	25.237205321838896	25.256657766401357	25.93787928728919
122-123	23.390847599972826	25.369488294734328	25.18729145524869	26.052372650044152
124-125	23.299640437965568	25.336488913697124	25.182015561702663	26.18185508663464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	22.0
2	23.0
3	43.0
4	51.0
5	61.5
6	147.0
7	443.5
8	754.5
9	809.0
10	673.0
11	520.5
12	498.5
13	540.0
14	591.5
15	637.0
16	686.5
17	782.5
18	879.5
19	920.0
20	946.0
21	1050.5
22	1180.0
23	1338.0
24	1518.5
25	1755.0
26	2151.0
27	2758.5
28	3604.5
29	4817.5
30	6586.5
31	8788.0
32	11545.5
33	14190.5
34	16823.0
35	20267.0
36	24312.0
37	28062.0
38	31684.0
39	35658.0
40	39526.5
41	43379.0
42	47639.0
43	53168.5
44	59216.5
45	64773.5
46	70378.5
47	75464.5
48	80745.5
49	85578.0
50	87887.0
51	88766.5
52	88288.0
53	88119.0
54	88816.5
55	87061.5
56	82414.5
57	75691.0
58	69498.5
59	63391.5
60	54907.5
61	47249.5
62	40065.0
63	31219.5
64	23329.0
65	17814.5
66	13648.5
67	10695.0
68	8514.5
69	6585.0
70	5191.0
71	3999.0
72	3045.5
73	2546.5
74	2063.0
75	1606.0
76	1185.0
77	720.0
78	389.5
79	217.5
80	135.5
81	70.0
82	41.5
83	26.5
84	15.0
85	9.0
86	7.0
87	5.5
88	7.0
89	4.5
90	1.5
91	1.0
92	1.0
93	1.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023054102523858165
2	0.002573002513823456
3	0.03231691157362261
4	0.16801706415267167
5	0.025627105037681626
6	0.07050026887876269
7	0.050893989723427964
8	0.04286622188029878
9	0.033809253031640216
10-11	0.06571448420305107
12-13	0.05727503595771013
14-15	0.05907613771738655
16-17	0.04978759864248388
18-19	0.03620214536949603
20-21	0.024006113453972844
22-23	0.0031133330417263817
24-25	0.0053518452287527885
26-27	0.003525013443938135
28-29	0.0047600546505733934
30-31	0.0025987325389616907
32-33	0.009545839326285022
34-35	0.004451294348914579
36-37	0.006432506284558641
38-39	0.0023671623127175796
40-41	0.003576473494214604
42-43	0.00411680402211753
44-45	0.005634875505273368
46-47	0.004862974751126331
48-49	0.0022385121870264067
50-51	0.0016981816591234808
52-53	9.262809049764441E-4
54-55	0.005274655153338085
56-57	8.23360804423506E-4
58-59	8.23360804423506E-4
60-61	1.0292010055293825E-4
62-63	0.0010806610558058516
64-65	0.0
66-67	0.003036142966311678
68-69	3.859503770735184E-4
70-71	0.0
72-73	0.0023157022624411107
74-75	0.004862974751126331
76-77	0.0019040218602293575
78-79	2.830302765205802E-4
80-81	0.0
82-83	0.005634875505273368
84-85	0.002084132036196999
86-87	0.0
88-89	0.001235041206635259
90-91	7.976307792852713E-4
92-93	0.0
94-95	0.0036279335444910727
96-97	0.0014151513826029007
98-99	5.660605530411604E-4
100-101	0.0
102-103	0.001106391080944086
104-105	0.0
106-107	6.689806535940986E-4
108-109	0.0028303027652058013
110-111	5.146005027646912E-5
112-113	4.11680402211753E-4
114-115	0.0
116-117	0.0018525618099528881
118-119	0.002470082413270518
120-121	0.002804572740067567
122-123	0.0013894213574646662
124-125	0.11030461776761157
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1943255.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.94326730703108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.32834849679365	47.552003989686185
2	11.207325076692173	13.43607365737903
3	3.7105881063023336	6.672743241671131
4	1.6879489111107309	4.047246911172903
5	1.0095912766925554	3.025909988481432
6	0.641213936673029	2.3061875044191047
7	0.4382515450564148	1.8389160659125232
8	0.34097397161029075	1.6351275139980546
9	0.24308303456603933	1.3114072186916734
>10	1.3155660155602722	13.71221669056433
>50	0.05970002671422753	2.3759190720698893
>100	0.016806637022963727	1.5986695725013143
>500	3.445515459156344E-4	0.1448846211063697
>1k	2.584136594367256E-4	0.34269395234603534
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2839	0.14609508273489583	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2290	0.1178435151331143	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	1.0292010055293825E-4
5	0.0	0.0	0.0	0.0	1.0292010055293825E-4
6	0.0	0.0	0.0	0.0	2.058402011058765E-4
7	0.0	0.0	0.0	0.0	2.058402011058765E-4
8	0.0	0.0	0.0	0.0	2.058402011058765E-4
9	0.0	0.0	0.0	5.146005027646912E-5	3.087603016588147E-4
10-11	0.0	0.0	0.0	7.719007541470368E-5	3.087603016588147E-4
12-13	0.0	0.0	0.0	1.0292010055293825E-4	3.087603016588147E-4
14-15	0.0	0.0	0.0	1.0292010055293825E-4	3.344903267970493E-4
16-17	0.0	0.0	0.0	1.8011017596764194E-4	3.6022035193528383E-4
18-19	0.0	0.0	0.0	3.087603016588147E-4	4.11680402211753E-4
20-21	0.0	0.0	0.0	3.6022035193528383E-4	5.146005027646912E-4
22-23	0.0	0.0	0.0	6.43250628455864E-4	5.660605530411604E-4
24-25	0.0	0.0	0.0	0.001286501256911728	6.175206033176295E-4
26-27	0.0	0.0	0.0	0.001698181659123481	6.947106787323331E-4
28-29	0.0	0.0	0.0	0.004168264072393998	7.719007541470368E-4
30-31	0.0	0.0	0.0	0.013662643348402552	7.719007541470368E-4
32-33	0.0	0.0	0.0	0.03121052049267852	9.262809049764442E-4
34-35	0.0	0.0	0.0	0.05465057339361021	9.777409552529132E-4
36-37	0.0	0.0	0.0	0.08321090129705057	0.0010292010055293825
38-39	0.0	0.0	0.0	0.12404445119142882	0.0010292010055293825
40-41	0.0	0.0	0.0	0.17156780762174806	0.0010292010055293825
42-43	0.0	0.0	0.0	0.22161270651561427	0.0010292010055293825
44-45	0.0	0.0	0.0	0.28894818230237407	0.0010549310306676172
46-47	0.0	0.0	0.0	0.3593969911308603	0.0010806610558058516
48-49	0.0	0.0	0.0	0.42709268726955546	0.0011321211060823207
50-51	0.0	0.0	0.0	0.4941451327797947	0.0011321211060823207
52-53	0.0	0.0	0.0	0.5655202225132574	0.0012607712317734936
54-55	0.0	0.0	0.0	0.652899387882702	0.0013894213574646662
56-57	0.0	0.0	0.0	0.7526032352933609	0.0014151513826029007
58-59	0.0	0.0	0.0	0.8465950171233317	0.00146661143287937
60-61	0.0	0.0	0.0	0.9405096088778879	0.0015180714831558389
62-63	0.0	0.0	0.0	1.039029875132188	0.0017496417093999502
64-65	0.0	0.0	0.0	1.1551237485559023	0.0017496417093999502
66-67	0.0	0.0	0.0	1.2998036799081953	0.0017496417093999502
68-69	0.0	0.0	0.0	1.4451783219392205	0.0017496417093999502
70-71	0.0	0.0	0.0	1.5903985838194163	0.001826831784814654
72-73	0.0	0.0	0.0	1.7396069995960386	0.002058402011058765
74-75	0.0	0.0	0.0	1.9020149182685753	0.002109862061335234
76-77	0.0	0.0	0.0	2.105642337212563	0.0021355920864734685
78-79	0.0	0.0	0.0	2.3183781850554865	0.002187052136749938
80-81	0.0	0.0	0.0	2.5301620219682954	0.0022385121870264067
82-83	0.0	0.0	0.0	2.743232360138016	0.0025472724886852214
84-85	0.0	0.0	0.0	2.9756259471865505	0.0025987325389616907
86-87	0.0	0.0	0.0	3.2535616787297603	0.0027273826646528635
88-89	0.0	0.0	0.0	3.565975643958204	0.0028303027652058018
90-91	0.0	0.0	0.0	3.8834584241388805	0.0028560327903440364
92-93	0.0	0.0	0.0	4.184937128683575	0.0029074928406205053
94-95	0.0	0.0	0.0	4.508183434495216	0.003036142966311678
96-97	0.0	0.0	0.0	4.87656020439932	0.0031133330417263817
98-99	0.0	0.0	0.0	5.289990248320473	0.0031390630668646164
100-101	0.0	2.573002513823456E-5	0.0	5.7282497665000225	0.0031390630668646164
102-103	0.0	5.146005027646912E-5	0.0	6.1545962830405685	0.0031905231171410853
104-105	0.0	5.146005027646912E-5	0.0	6.590591559007953	0.00321625314227932
106-107	0.0	5.146005027646912E-5	0.0	7.087952944930027	0.0034220933433851963
108-109	0.0	5.146005027646912E-5	0.0	7.639630413918914	0.0036022035193528385
110-111	0.0	5.146005027646912E-5	0.0	8.225065675889166	0.0037308536450440114
112-113	0.0	5.146005027646912E-5	0.0	8.791820939609057	0.003833773745596949
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3195	0.0	58.62385	1
GTATCAA	5690	0.0	51.3688	1
TCAACGC	7355	0.0	39.23932	4
CAACGCA	7605	0.0	37.916145	5
TATCAAC	7715	0.0	37.49238	2
ATCAACG	7705	0.0	37.462666	3
AACGCAG	7955	0.0	36.33312	6
ACGCAGA	9400	0.0	30.621073	7
GTACATG	11520	0.0	30.239594	1
CGCAGAG	9855	0.0	29.084127	8
TACATGG	11885	0.0	28.95435	2
ACATGGG	12025	0.0	28.31894	3
CATGGGG	10255	0.0	25.817017	4
GCAGAGT	11010	0.0	25.816576	9
TAGGTCG	170	1.38547275E-5	24.53167	5
ATGGGGG	5750	0.0	23.934378	5
AGAGTAC	9885	0.0	23.449873	10-11
GAGTACT	4045	0.0	22.814821	12-13
CAGAGTA	10355	0.0	22.644472	10-11
GTACTTT	4165	0.0	22.079193	14-15
>>END_MODULE