FastQCFastQC Report
Mon 15 Aug 2016
SRR2921589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861771
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.047.324521
GTATCAA34350.042.800081
AGCCTCG12950.040.9001162
GTACATG101000.036.773721
TACATGG103800.035.489312
ACATGGG103200.035.1189773
TCAACGC43950.033.0396354
CATGGGG91650.032.4669764
CAACGCA44750.032.3159945
ATGGGGG56350.031.049725
ATCAACG47600.030.5061343
AACGCAG47650.030.3435156
TATCAAC47400.030.132642
ACGCAGA57850.025.5035617
CGCAGAG62450.023.4332148
GCCTCGG19000.021.925463
TCGGTCT8600.021.447956
CCTCGGT13100.021.3516224
CGGTCTC8400.020.5386187
TGGGGGG45550.020.3778696
Produced by FastQC (version 0.11.5)