##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921589_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1861771
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.69070309936077	31.0	31.0	34.0	25.0	34.0
2	29.644693144323334	31.0	31.0	34.0	25.0	34.0
3	29.76076381037195	31.0	31.0	34.0	25.0	34.0
4	33.298589353900134	37.0	35.0	37.0	27.0	37.0
5	33.24793167365911	37.0	35.0	37.0	27.0	37.0
6	33.340082641742725	37.0	35.0	37.0	27.0	37.0
7	33.143878597314064	37.0	35.0	37.0	26.0	37.0
8	33.153332499002296	37.0	35.0	37.0	26.0	37.0
9	34.81175504398769	39.0	37.0	39.0	27.0	39.0
10-11	34.86600849406291	39.0	37.0	39.0	27.0	39.0
12-13	34.80829973181449	39.0	37.0	39.0	27.0	39.0
14-15	36.054425329430956	40.0	37.5	41.0	26.5	41.0
16-17	36.00181842987134	40.0	37.5	41.0	26.0	41.0
18-19	35.94418137354164	40.0	37.0	41.0	25.0	41.0
20-21	36.02217297401238	40.0	37.5	41.0	26.0	41.0
22-23	35.928030622455715	40.0	37.0	41.0	25.5	41.0
24-25	35.92188298131188	40.0	37.0	41.0	25.5	41.0
26-27	35.89703996893281	40.0	37.0	41.0	25.5	41.0
28-29	35.837063473434704	40.0	37.0	41.0	25.0	41.0
30-31	35.73376398063994	40.0	37.0	41.0	25.0	41.0
32-33	35.64737446227275	40.0	36.5	41.0	25.0	41.0
34-35	35.511940512554986	40.0	36.0	41.0	24.0	41.0
36-37	35.39296186265658	40.0	36.0	41.0	24.0	41.0
38-39	35.262642666579296	40.0	36.0	41.0	23.0	41.0
40-41	35.147514382810776	40.0	35.0	41.0	22.0	41.0
42-43	34.85881856576346	40.0	35.0	41.0	20.0	41.0
44-45	34.43698634257382	39.0	35.0	41.0	17.0	41.0
46-47	34.15252412890737	39.0	34.5	41.0	15.0	41.0
48-49	33.93103689981206	39.0	34.0	40.0	12.0	41.0
50-51	33.715956742263145	38.0	34.0	40.0	9.0	41.0
52-53	33.50838314701432	38.0	33.0	40.0	8.0	41.0
54-55	33.2594814292413	38.0	33.0	40.0	7.0	41.0
56-57	33.00082904932991	37.5	33.0	40.0	4.0	41.0
58-59	33.00443448737788	37.0	33.0	40.0	2.0	41.0
60-61	32.78910403051718	37.0	33.0	40.0	2.0	41.0
62-63	32.480832229098	36.5	33.0	40.0	2.0	41.0
64-65	32.18149197726251	36.0	32.0	39.0	2.0	41.0
66-67	31.858765122026284	35.5	32.0	39.0	2.0	41.0
68-69	31.528975905199943	35.0	32.0	38.5	2.0	40.0
70-71	31.16893377327287	35.0	31.5	37.5	2.0	40.0
72-73	30.838670545410793	35.0	31.0	37.0	2.0	39.0
74-75	30.4870494276686	35.0	31.0	36.5	2.0	39.0
76-77	30.202872694869562	35.0	31.0	36.0	2.0	38.5
78-79	29.94277249994763	35.0	31.0	35.5	2.0	37.0
80-81	29.69711151371463	35.0	31.0	35.0	2.0	37.0
82-83	29.48984219863775	35.0	30.0	35.0	2.0	36.5
84-85	29.293367712785297	34.0	30.0	35.0	2.0	36.0
86-87	29.133032204282912	34.0	30.0	35.0	2.0	36.0
88-89	28.982990389258397	34.0	30.0	35.0	2.0	35.0
90-91	28.804192889458477	34.0	30.0	35.0	2.0	35.0
92-93	28.729799744436882	34.0	29.5	35.0	2.0	35.0
94-95	28.609684542298705	34.0	29.0	35.0	2.0	35.0
96-97	28.521975312753288	34.0	29.0	35.0	2.0	35.0
98-99	28.427642013974868	34.0	29.0	35.0	2.0	35.0
100-101	28.321979180038788	34.0	29.0	35.0	2.0	35.0
102-103	27.422902709302058	32.5	27.0	34.5	2.0	35.0
104-105	28.357373704929337	34.0	29.5	35.0	2.0	35.0
106-107	28.65313859760411	34.0	30.0	35.0	2.0	35.0
108-109	28.658929320523306	34.0	30.0	35.0	2.0	35.0
110-111	28.594907483251163	34.0	30.0	35.0	2.0	35.0
112-113	28.514566775398265	34.0	30.0	35.0	2.0	35.0
114-115	28.399610370985474	34.0	30.0	35.0	2.0	35.0
116-117	28.279760239041217	34.0	29.5	35.0	2.0	35.0
118-119	27.993987713848803	34.0	29.0	35.0	2.0	35.0
120-121	27.860458402241736	34.0	29.0	35.0	2.0	35.0
122-123	27.6131358260495	34.0	29.0	35.0	2.0	35.0
124-125	26.831290744135558	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97180.0
3	15633.0
4	7621.0
5	6636.0
6	6836.0
7	7458.0
8	7450.0
9	7685.0
10	8470.0
11	9091.0
12	9258.0
13	9227.0
14	9200.0
15	7928.0
16	6434.0
17	5886.0
18	5425.0
19	5308.0
20	5154.0
21	5382.0
22	6263.0
23	8357.0
24	11769.0
25	14717.0
26	16476.0
27	18570.0
28	22474.0
29	26873.0
30	32573.0
31	41468.0
32	54505.0
33	75882.0
34	118510.0
35	192463.0
36	364408.0
37	496509.0
38	116614.0
39	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.9214007113155	17.42497340625571	12.208247821461958	25.445378060966828
2	16.496667339931324	20.01776988959892	38.154230608903426	25.331332161566326
3	20.769181183826728	23.31985023555133	27.84447703307388	28.06649154754806
4	13.86378006008929	14.817924507524053	33.80385192443124	37.51444350795541
5	14.812387420902523	37.36554302005566	31.951324464710396	15.870745094331411
6	34.514086949041825	33.20460954111572	17.270725865633004	15.010577644209445
7	30.148043605844784	31.36835803055677	18.907134426990737	19.576463936607713
8	28.766746379342774	34.15820727087084	18.367681435902945	18.70736491388344
9	28.267950943463134	15.903724370473885	17.95521471502691	37.87310997103608
10-11	26.964046236592942	25.18569093579956	25.793299246219608	22.056963581387887
12-13	26.601422651165752	22.668570840727586	26.236909586739255	24.493096921367403
14-15	24.468818712130673	24.031693711626982	23.505032229641063	27.99445534660128
16-17	25.15912273217197	26.899801304790284	24.040239809751217	23.90083615328653
18-19	24.979983707543965	26.2258299910155	25.37093279684296	23.423253504597568
20-21	25.52635226836793	25.001692451023693	25.49561950692186	23.976335773686525
22-23	25.887531161417147	24.53269628233782	25.103011672551705	24.476760883693327
24-25	25.298962115078048	24.98166494644015	25.047590545613584	24.671782392868217
26-27	24.707878213379956	25.340418037299482	25.35970966900343	24.591994080317132
28-29	24.961330078710116	25.546114401707737	25.198951606511216	24.29360391307093
30-31	25.519709275493597	25.03359245594835	25.193213706044386	24.253484562513666
32-33	24.864303772576747	25.276503310034283	25.20082773019735	24.65836518719162
34-35	24.93549594436544	25.728647968662973	25.23695819750291	24.098897889468677
36-37	25.15999070688134	25.546084870883394	25.104527440887303	24.189396981347958
38-39	25.128963180085357	25.597216909568367	25.08926153872602	24.184558371620255
40-41	25.028862581869117	25.396895764501753	25.13343150775656	24.440810145872575
42-43	25.037714521487608	25.179493185598425	25.06035935169705	24.722432941216923
44-45	24.719663986260333	25.314892008822053	25.41389970246657	24.551544302451045
46-47	24.665832142435022	25.259517336972287	25.4099197352445	24.66473078534819
48-49	24.727477870757163	25.58460153930879	25.204609867157384	24.483310722776665
50-51	24.732820613249327	25.565057237087235	25.31946952840014	24.382652621263297
52-53	24.85048116804133	25.403346256173094	25.209020127824367	24.537152447961212
54-55	24.55421288666648	25.21916305032524	25.406312916617978	24.820311146390306
56-57	24.695317528456194	25.44735295350194	25.010960187655606	24.84636933038626
58-59	24.654354732825986	25.50143812928229	25.27763362461226	24.56657351327946
60-61	24.944368035931102	25.368336789624678	25.467002267183858	24.22029290726036
62-63	24.748446409105732	25.41106293178061	25.331469436151405	24.509021222962254
64-65	24.60676897818493	25.230071979086414	25.316352328978486	24.846806713750166
66-67	24.65243526344859	25.459613933966462	25.14399033760362	24.743960464981335
68-69	24.572824176821914	25.49656083504848	25.252195580311742	24.678419407817863
70-71	24.508578234796413	25.38710404607659	25.429852144242993	24.674465574884003
72-73	24.638726829211947	25.72448711794575	25.098200598108328	24.538585454733976
74-75	24.567446725019963	25.650283641711948	25.17458851740567	24.607681115862423
76-77	24.660288369102137	25.3095875648715	25.08664530181413	24.943478764212234
78-79	24.548000888619306	25.451770778124818	25.307302983993303	24.69292534926258
80-81	24.722665207568294	25.65206144652198	25.062082862927216	24.563190482982513
82-83	24.723085560477404	25.598971694742694	25.197400762364243	24.48054198241566
84-85	24.684408734570283	25.664857506840416	24.951176320965562	24.69955743762374
86-87	24.400694084434406	25.841375920319965	25.0715976029375	24.686332392308127
88-89	24.443984801333443	25.592937374670772	25.107240856422564	24.85583696757322
90-91	24.709229314982167	25.370831843630793	25.189273926816497	24.73066491457054
92-93	24.698025512691213	25.544894305907885	25.192943252458537	24.56413692894236
94-95	24.715984861120848	25.39364067159639	25.167809119694752	24.72256534758801
96-97	24.473610627251404	25.39655977761763	25.23034781223635	24.899481782894618
98-99	24.358375218422886	25.463717225823885	25.086361978491016	25.09154557726221
100-101	24.36612330221551	25.689702916419677	25.155774891281897	24.788398890082917
102-103	24.22092543573172	25.478005001890747	25.397433308123347	24.903636254254184
104-105	24.249530062440797	25.54249957095285	25.296834827187325	24.91113553941903
106-107	24.266591755189868	25.49062630757141	25.309990100727646	24.932791836511072
108-109	24.23845303831788	25.539200228631962	25.312233582790718	24.91011315025944
110-111	24.115116855548806	25.95989349765247	25.256869503470675	24.668120143328053
112-113	24.036229840604815	26.015012958412225	25.257328552820635	24.691428648162322
114-115	24.113796160828656	26.05183448055797	25.177710813439436	24.656658545173933
116-117	24.0327739054953	25.934889432249857	25.206267081492683	24.826069580762155
118-119	24.053188624367124	25.795944199073574	25.270979209307338	24.879887967251964
120-121	24.01379670777229	26.023762070977902	25.31238779029498	24.650053430954824
122-123	23.94655452832499	26.055030004060697	25.248879010478326	24.74953645713598
124-125	23.920407644245586	25.977164034027112	25.220859384934364	24.88156893679294
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	638.0
1	522.5
2	366.5
3	291.0
4	239.5
5	208.5
6	213.0
7	285.5
8	357.0
9	363.5
10	356.5
11	370.0
12	393.0
13	435.5
14	493.0
15	537.0
16	575.0
17	622.0
18	708.5
19	816.5
20	895.0
21	969.5
22	1100.0
23	1332.5
24	1605.0
25	2000.0
26	2637.0
27	3493.0
28	4561.0
29	5988.0
30	7949.5
31	10145.0
32	13046.0
33	15608.0
34	17716.0
35	20525.5
36	24014.5
37	27554.5
38	30993.5
39	34945.5
40	39028.5
41	42675.0
42	46479.5
43	51651.5
44	57085.5
45	62353.0
46	67388.5
47	72007.5
48	77731.0
49	82658.0
50	84399.0
51	85027.0
52	84863.0
53	84210.5
54	83467.5
55	80877.0
56	75942.0
57	68855.0
58	62871.0
59	56920.0
60	49482.5
61	43139.5
62	36672.0
63	28939.0
64	21787.0
65	16884.5
66	13292.5
67	10452.0
68	8318.0
69	6594.5
70	5316.5
71	4139.5
72	3184.0
73	2715.5
74	2239.0
75	1728.5
76	1303.5
77	918.0
78	605.5
79	438.0
80	342.0
81	246.0
82	180.5
83	140.5
84	119.0
85	94.5
86	79.5
87	61.5
88	48.5
89	47.5
90	37.0
91	38.5
92	40.5
93	25.0
94	20.5
95	18.5
96	10.0
97	7.5
98	7.5
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023150000725116034
2	0.010473898239901684
3	0.00972192605857541
4	0.011172158122561797
5	0.016221114197181072
6	0.09217030451113484
7	0.059244665428777224
8	0.04946902707153565
9	0.02631902634641962
10-11	0.05105353988218744
12-13	0.013240081621209054
14-15	0.027849826858405247
16-17	0.0337850358610162
18-19	0.042271579050269875
20-21	0.030347448746381803
22-23	0.03735690372231601
24-25	0.03126055782370657
26-27	0.046219433002232815
28-29	0.04450063944491562
30-31	0.02631902634641962
32-33	0.028816648234396173
34-35	0.04519889932757573
36-37	0.009587645311910004
38-39	0.020786659583804882
40-41	0.04283555818626458
42-43	0.022505453141122084
44-45	0.016086833450515665
46-47	0.023150000725116034
48-49	0.02932691507172472
50-51	0.028010963754403737
52-53	0.02508364347709788
54-55	0.023015719978450627
56-57	0.026265314047753455
58-59	0.017456497066502808
60-61	0.023150000725116034
62-63	0.02352598681577917
64-65	0.02132378257046651
66-67	0.02873607978639693
68-69	0.022451740842455922
70-71	0.046702843690228285
72-73	0.0216192002131304
74-75	0.009641357610576166
76-77	0.01624797034651415
78-79	0.024519664341103174
80-81	0.018665023786491465
82-83	0.02449280819177009
84-85	0.012112123349219642
86-87	0.01751020936516897
88-89	0.022747158485119812
90-91	0.02049124194114099
92-93	0.007976276351925129
94-95	0.011091589674562554
96-97	0.015710847359852526
98-99	0.006982598826601124
100-101	0.010232192895903953
102-103	0.004028422399962187
104-105	0.004699826133289217
106-107	9.668213759909248E-4
108-109	0.014180046847866898
110-111	0.0015039443626525496
112-113	0.0024707657386434743
114-115	0.01340121851720754
116-117	0.005747215957279386
118-119	0.2777462964027262
120-121	0.0012085267199886558
122-123	0.0012353828693217371
124-125	0.3136261119117228
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1861771.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.67090563633639
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.95057013742564	52.178670180639955
2	9.869833426777658	12.568424655254379
3	3.205243385460535	6.122422474114474
4	1.5056460290000784	3.834633849367544
5	0.854947718773724	2.721764776302142
6	0.5598236737847008	2.1386688183919715
7	0.3686912792053355	1.643243535305615
8	0.2932920980781843	1.4939338800495352
9	0.20596763337808968	1.1802731174060288
>10	1.125529101357651	12.499142982533144
>50	0.04611061669784189	1.9832022608786284
>100	0.013838858808150651	1.409863935254378
>500	4.2170104365873333E-4	0.16657794196617662
>1k	8.43402087317461E-5	0.059177592536017294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.371229866616249E-5	0.0
3	0.0	0.0	0.0	5.371229866616249E-5	0.0
4	0.0	0.0	0.0	5.371229866616249E-5	0.0
5	0.0	0.0	0.0	5.371229866616249E-5	0.0
6	0.0	0.0	0.0	5.371229866616249E-5	0.0
7	0.0	0.0	0.0	5.371229866616249E-5	0.0
8	0.0	0.0	0.0	5.371229866616249E-5	0.0
9	0.0	0.0	0.0	1.0742459733232498E-4	0.0
10-11	0.0	0.0	0.0	1.6113689599848746E-4	0.0
12-13	0.0	0.0	0.0	1.6113689599848746E-4	5.371229866616249E-5
14-15	0.0	0.0	0.0	1.6113689599848746E-4	5.371229866616249E-5
16-17	0.0	0.0	0.0	1.6113689599848746E-4	8.056844799924373E-5
18-19	0.0	0.0	0.0	1.6113689599848746E-4	1.6113689599848746E-4
20-21	0.0	0.0	0.0	1.6113689599848746E-4	2.1484919466464996E-4
22-23	0.0	0.0	0.0	2.1484919466464996E-4	3.759860906631374E-4
24-25	0.0	0.0	0.0	6.176914346608686E-4	4.834106879954624E-4
26-27	0.0	0.0	0.0	9.668213759909249E-4	4.834106879954624E-4
28-29	0.0	0.0	0.0	0.003625580159965968	4.834106879954624E-4
30-31	0.0	0.0	0.0	0.011521288063891853	4.834106879954624E-4
32-33	0.0	0.0	0.0	0.028682367487730766	5.371229866616249E-4
34-35	0.0	0.0	0.0	0.05180551206351372	5.371229866616249E-4
36-37	2.6856149333081245E-5	0.0	0.0	0.08201868006323011	5.371229866616249E-4
38-39	5.371229866616249E-5	0.0	0.0	0.13049402960944176	6.445475839939499E-4
40-41	5.371229866616249E-5	0.0	0.0	0.18372291758760878	6.445475839939499E-4
42-43	5.371229866616249E-5	0.0	0.0	0.23961056434975087	6.714037333270311E-4
44-45	5.371229866616249E-5	0.0	0.0	0.30352819976248424	7.788283306593561E-4
46-47	5.371229866616249E-5	0.0	0.0	0.3729513457884992	9.131090773247623E-4
48-49	5.371229866616249E-5	0.0	0.0	0.44637605806514336	9.131090773247623E-4
50-51	5.371229866616249E-5	0.0	0.0	0.5187533805177973	9.131090773247623E-4
52-53	5.371229866616249E-5	0.0	0.0	0.5921243804957752	9.936775253240062E-4
54-55	5.371229866616249E-5	0.0	0.0	0.6747607519936663	0.0010742459733232498
56-57	5.371229866616249E-5	0.0	0.0	0.7694555345421108	0.0010742459733232498
58-59	5.371229866616249E-5	0.0	0.0	0.8719117442478157	0.0010742459733232498
60-61	5.371229866616249E-5	0.0	0.0	0.9756570491215085	0.0010742459733232498
62-63	5.371229866616249E-5	0.0	0.0	1.084558734667153	0.0011816705706555748
64-65	5.371229866616249E-5	0.0	0.0	1.1994761976634076	0.0011816705706555748
66-67	5.371229866616249E-5	0.0	0.0	1.327875447624869	0.0011816705706555748
68-69	5.371229866616249E-5	0.0	0.0	1.4671245819168952	0.0011816705706555748
70-71	5.371229866616249E-5	0.0	0.0	1.615101964742173	0.001208526719988656
72-73	5.371229866616249E-5	0.0	0.0	1.759292630511486	0.0012622390186548184
74-75	5.371229866616249E-5	0.0	0.0	1.9115938533793897	0.0013159513173209808
76-77	5.371229866616249E-5	0.0	0.0	2.0932488474683515	0.001342807466654062
78-79	5.371229866616249E-5	0.0	0.0	2.297651000042433	0.001342807466654062
80-81	5.371229866616249E-5	0.0	0.0	2.4999046606698676	0.0013696636159871434
82-83	5.371229866616249E-5	0.0	0.0	2.696787091430686	0.0015039443626525496
84-85	5.371229866616249E-5	0.0	0.0	2.908977527311361	0.0015308005119856309
86-87	5.371229866616249E-5	0.0	0.0	3.1533684862424005	0.0015845128106517932
88-89	5.371229866616249E-5	0.0	0.0	3.428187462367821	0.0016113689599848745
90-91	5.371229866616249E-5	0.0	0.0	3.7180190259704333	0.0016113689599848745
92-93	5.371229866616249E-5	0.0	0.0	3.996436726106487	0.0016113689599848745
94-95	5.371229866616249E-5	0.0	0.0	4.285543173677106	0.0017993620053164433
96-97	5.371229866616249E-5	0.0	0.0	4.611496258132714	0.001987355050648012
98-99	5.371229866616249E-5	0.0	0.0	4.989577128443831	0.0020410673493141745
100-101	5.371229866616249E-5	0.0	0.0	5.3886595075334185	0.002094779647980337
102-103	5.371229866616249E-5	0.0	0.0	5.7895143924789885	0.0021484919466464997
104-105	5.371229866616249E-5	0.0	0.0	6.205918987888413	0.002202204245312662
106-107	5.371229866616249E-5	0.0	0.0	6.656054906860188	0.002202204245312662
108-109	5.371229866616249E-5	0.0	0.0	7.143332880359615	0.002229060394645743
110-111	5.371229866616249E-5	0.0	0.0	7.6635364929413985	0.0022559165439788244
112-113	5.371229866616249E-5	0.0	0.0	8.182316729608528	0.0022559165439788244
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2440	0.0	47.32452	1
GTATCAA	3435	0.0	42.80008	1
AGCCTCG	1295	0.0	40.900116	2
GTACATG	10100	0.0	36.77372	1
TACATGG	10380	0.0	35.48931	2
ACATGGG	10320	0.0	35.118977	3
TCAACGC	4395	0.0	33.039635	4
CATGGGG	9165	0.0	32.466976	4
CAACGCA	4475	0.0	32.315994	5
ATGGGGG	5635	0.0	31.04972	5
ATCAACG	4760	0.0	30.506134	3
AACGCAG	4765	0.0	30.343515	6
TATCAAC	4740	0.0	30.13264	2
ACGCAGA	5785	0.0	25.503561	7
CGCAGAG	6245	0.0	23.433214	8
GCCTCGG	1900	0.0	21.92546	3
TCGGTCT	860	0.0	21.44795	6
CCTCGGT	1310	0.0	21.351622	4
CGGTCTC	840	0.0	20.538618	7
TGGGGGG	4555	0.0	20.377869	6
>>END_MODULE