FastQCFastQC Report
Mon 15 Aug 2016
SRR2921589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861771
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32310.173544436990371No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.1474939721372822No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.058.3345221
GTATCAA51050.055.511551
TCAACGC66600.042.3576664
CAACGCA67450.041.9555325
TATCAAC68900.041.1223562
AACGCAG69500.040.8146936
ATCAACG69350.040.596393
GTACATG98750.037.1555331
TACATGG102650.035.679032
ACATGGG105050.034.8061753
ACGCAGA81750.034.623977
CATGGGG90150.033.2110864
CGCAGAG87200.032.5239338
ATGGGGG56500.030.052045
GCAGAGT97800.028.7551569
GTGGTAT15650.025.1602571
AGAGTAC89050.024.8563610-11
GTATAGG4850.024.6021881
GAGTACT45500.024.46831912-13
CAGAGTA94100.023.83899710-11
Produced by FastQC (version 0.11.5)