##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1861771
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.62235903341496	33.0	31.0	34.0	30.0	34.0
2	31.791676312500304	33.0	31.0	34.0	30.0	34.0
3	31.850404802738897	34.0	31.0	34.0	30.0	34.0
4	35.244622996061274	37.0	35.0	37.0	33.0	37.0
5	35.076845111455704	37.0	35.0	37.0	33.0	37.0
6	35.09628627795792	37.0	35.0	37.0	33.0	37.0
7	34.983222426388636	37.0	35.0	37.0	32.0	37.0
8	35.01616149354566	37.0	35.0	37.0	33.0	37.0
9	36.719322623459064	39.0	38.0	39.0	33.0	39.0
10-11	36.66336541927015	39.0	38.0	39.0	33.0	39.0
12-13	36.58956794364076	39.0	38.0	39.0	33.0	39.0
14-15	38.00016731381035	41.0	39.0	41.0	33.0	41.0
16-17	38.00440225999868	41.0	39.0	41.0	33.0	41.0
18-19	38.01735498082202	41.0	39.0	41.0	33.0	41.0
20-21	37.960520654795886	41.0	39.0	41.0	33.0	41.0
22-23	37.963013174015494	41.0	39.0	41.0	33.0	41.0
24-25	37.922201763804466	41.0	39.0	41.0	33.0	41.0
26-27	37.838206471150315	41.0	39.0	41.0	33.0	41.0
28-29	37.76485829889927	41.0	38.0	41.0	33.0	41.0
30-31	37.64363474347812	41.0	38.0	41.0	32.5	41.0
32-33	37.51347963847326	41.0	38.0	41.0	32.0	41.0
34-35	37.375060090634136	40.0	38.0	41.0	32.0	41.0
36-37	37.2904522092137	40.0	38.0	41.0	31.5	41.0
38-39	37.20023622668953	40.0	38.0	41.0	31.0	41.0
40-41	37.07230454228797	40.0	37.5	41.0	31.0	41.0
42-43	36.90128189771997	40.0	37.0	41.0	30.5	41.0
44-45	36.73391679212965	40.0	37.0	41.0	30.0	41.0
46-47	36.51068955311905	40.0	36.5	41.0	30.0	41.0
48-49	36.30280039811556	40.0	36.0	41.0	29.5	41.0
50-51	36.05049412629158	40.0	35.5	41.0	28.5	41.0
52-53	35.91426926297595	40.0	35.0	41.0	28.0	41.0
54-55	35.77975406212687	39.5	35.0	41.0	28.0	41.0
56-57	35.57811809293409	39.0	35.0	41.0	28.0	41.0
58-59	35.382056654658385	39.0	35.0	41.0	28.0	41.0
60-61	35.12309999457506	38.5	35.0	41.0	28.0	41.0
62-63	34.77375708398079	37.5	35.0	40.5	27.5	41.0
64-65	34.486657059326845	37.0	35.0	40.0	27.0	41.0
66-67	34.184891965768074	36.5	35.0	39.5	26.5	41.0
68-69	33.82242015801084	36.0	35.0	39.0	26.0	41.0
70-71	33.45709112452606	35.0	34.0	39.0	26.0	41.0
72-73	33.151269946733514	35.0	34.0	37.0	26.0	39.5
74-75	32.83524047801797	35.0	34.0	37.0	26.0	39.0
76-77	32.521202929898465	35.0	34.0	36.5	25.0	39.0
78-79	32.26725252461232	35.0	34.0	36.0	25.0	38.0
80-81	32.024681069798596	35.0	34.0	36.0	25.0	37.0
82-83	31.810277687212874	35.0	34.0	35.0	24.0	37.0
84-85	31.604957322893092	35.0	33.5	35.0	23.5	36.0
86-87	31.482985286590026	35.0	33.0	35.0	23.5	36.0
88-89	31.39002782834194	35.0	33.0	35.0	23.0	36.0
90-91	31.28604645791561	35.0	33.0	35.0	23.0	35.5
92-93	31.2129303227948	35.0	33.0	35.0	23.0	35.0
94-95	31.14804291182965	35.0	33.0	35.0	21.5	35.0
96-97	31.083420302496926	35.0	33.0	35.0	20.0	35.0
98-99	31.011808648861756	35.0	33.0	35.0	20.0	35.0
100-101	30.941737463952332	35.0	33.0	35.0	19.5	35.0
102-103	30.253902332778843	34.0	31.5	35.0	19.0	35.0
104-105	30.76807029435951	35.0	33.0	35.0	19.0	35.0
106-107	30.86896347617403	35.0	33.0	35.0	19.0	35.0
108-109	30.855328609157624	35.0	33.0	35.0	19.0	35.0
110-111	30.786088353508568	35.0	33.0	35.0	18.0	35.0
112-113	30.747450680024556	35.0	33.0	35.0	18.0	35.0
114-115	30.624269311317022	35.0	33.0	35.0	15.0	35.0
116-117	30.81258919598597	35.0	33.0	35.0	17.0	35.0
118-119	30.79833851746536	35.0	33.0	35.0	11.0	35.0
120-121	30.717633908788997	35.0	33.0	35.0	2.0	35.0
122-123	30.58242528216413	35.0	33.0	35.0	2.0	35.0
124-125	29.940213646039176	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	35486.0
3	9774.0
4	5133.0
5	3868.0
6	4133.0
7	4588.0
8	5010.0
9	5231.0
10	5436.0
11	5952.0
12	6481.0
13	8367.0
14	11343.0
15	10365.0
16	5822.0
17	5079.0
18	4680.0
19	4193.0
20	3952.0
21	3992.0
22	4523.0
23	5538.0
24	7429.0
25	9670.0
26	11400.0
27	13261.0
28	15722.0
29	19403.0
30	23167.0
31	29144.0
32	38041.0
33	51470.0
34	80914.0
35	146151.0
36	335987.0
37	680386.0
38	240274.0
39	406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84589265931922	17.846094665845747	11.403053522059006	26.90495915277603
2	15.959332492539206	19.443453730182434	37.41387052486609	27.183343252412268
3	18.921781066935882	23.275694861215808	28.327599574252943	29.474924497595367
4	13.441159886886494	14.847277399250213	33.92033202343188	37.791230690431405
5	14.871888269981262	37.31658414564959	32.26147342995169	15.550054154417452
6	35.01215539338809	33.50491886281632	16.312898046805923	15.170027696989665
7	30.585932650715264	31.692459655294535	18.841376478750544	18.880231215239654
8	28.563966844445638	34.275373806037315	18.127973991428995	19.03268535808805
9	28.23888651874841	15.13375293775032	18.167950661913967	38.459409881587305
10-11	27.226009819386704	24.861590719147806	25.700484519820165	22.211914941645325
12-13	27.092907989519034	22.15790077586121	26.46674797986679	24.282443254752966
14-15	24.544598314874705	23.412492929379788	23.988138824658023	28.054769931087485
16-17	24.65539459938178	26.582847168781143	24.611892365962607	24.149865865874474
18-19	24.623280016162795	25.6222515722189	26.49135118405385	23.263117227564447
20-21	25.34488890703607	24.489288270070123	26.516232531251948	23.649590291641864
22-23	25.171428786285265	23.985258682090514	26.3692280091486	24.47408452247562
24-25	24.69524141789251	24.418877910444795	26.29602801987561	24.589852651787083
26-27	24.33043975233482	24.751531793133935	26.520150592977306	24.39787786155394
28-29	24.413748131054852	25.187270519587567	26.285123881486516	24.113857467871064
30-31	24.994944198182058	24.613711227102858	26.26876454466227	24.122580030052816
32-33	24.623577706560894	24.617964273858163	26.247874147375978	24.510583872204965
34-35	24.680530831796194	24.883331310073366	26.378645762273038	24.057492095857402
36-37	24.692717504224763	24.824590334768985	26.347681240539266	24.135010920466986
38-39	24.775564630162037	24.901952621959982	26.291549112316382	24.030933635561595
40-41	24.73588712276495	24.75734595832635	26.269240437792472	24.237526481116227
42-43	24.621689040530782	24.618949602942696	26.107323644676683	24.65203771184984
44-45	24.473593177767583	24.77592795316913	26.198622962725025	24.55185590633826
46-47	24.508193793413326	24.80537105259836	25.976112480988313	24.710322673
48-49	24.509438720494682	25.13533859424043	25.798650118882033	24.556572566382854
50-51	24.57774738806311	25.21271755845738	25.788710674578258	24.420824378901255
52-53	24.811562006345632	25.037666113603684	25.596037712843756	24.55473416720693
54-55	24.34571601610917	24.905374065524413	25.86894311432328	24.87996680404314
56-57	24.531658426683848	25.05398802366906	25.536704470592408	24.877649079054688
58-59	24.566403975601528	25.082449042731703	25.76793092113037	24.583216060536397
60-61	24.780095076165402	24.96545626864321	25.94162384676307	24.31282480842832
62-63	24.590567220938336	25.016375699777495	25.85840491687268	24.53465216241149
64-65	24.405203432645585	24.75261458041832	25.9052267974955	24.93695518944059
66-67	24.457349059512463	25.058897971417434	25.71588026818586	24.76787270088425
68-69	24.39904101160546	25.086591753419558	25.777096699428792	24.737270535546195
70-71	24.30425116730253	24.93093941198998	25.891610729783633	24.873198690923857
72-73	24.450758181053008	25.252594971707698	25.587633622679608	24.70901322455969
74-75	24.374324530474823	25.24187986257485	25.609184881514857	24.77461072543547
76-77	24.34050287889209	24.842022827563778	25.573676889985475	25.24379740355866
78-79	24.278440857529432	25.010782767123917	25.733993566327047	24.976782809019603
80-81	24.43630285357329	25.207853167763382	25.50445248099793	24.851391497665396
82-83	24.43624268865277	25.199163542664323	25.567571109885208	24.797022658797708
84-85	24.40242505235713	25.232780425549745	25.324496047499828	25.040298474593296
86-87	24.113438226290988	25.374334437479153	25.48173217866215	25.03049515756771
88-89	24.062154307753417	25.09400412030916	25.530017803108734	25.313823768828687
90-91	24.23462043361214	24.946152986750146	25.623309391778516	25.1959171878592
92-93	24.259428254065618	25.110445914132296	25.595897669477075	25.03422816232501
94-95	24.277169913681508	24.96801327811528	25.518904662917425	25.235912145285788
96-97	24.038660638967286	24.88171673948839	25.662277489561504	25.41734513198282
98-99	23.889113288744603	24.90404220531584	25.49963582768096	25.707208678258592
100-101	23.920960204020794	25.11979185410021	25.55212214606415	25.407125795814846
102-103	23.852397180164033	24.925855631252386	25.745572890299634	25.476174298283944
104-105	23.95474523988181	24.977078276544216	25.552632412901477	25.515544070672497
106-107	24.023987005829976	24.842245877283727	25.55463706883494	25.579130048051358
108-109	23.934139521792574	24.816467106957614	25.601787805761706	25.647605565488107
110-111	23.76591832821365	25.234091877134055	25.521103853378158	25.478885941274132
112-113	23.728634214530008	25.217864206996847	25.512745281029993	25.540756297443156
114-115	23.65511118177262	25.27193194007211	25.48192017170748	25.591036706447785
116-117	23.559026747340454	25.1291469012935	25.504468543212212	25.807357808153842
118-119	23.649800412576464	24.950348213784544	25.511739038537307	25.88811233510168
120-121	23.524149379510124	25.09584579160768	25.627694257094735	25.752310571787458
122-123	23.423527462295425	25.159373458266405	25.459361077398686	25.957738002039488
124-125	23.27645840306595	25.10406458819781	25.409395991197897	26.21008101753834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	74.0
1	57.0
2	49.5
3	78.5
4	79.0
5	79.0
6	190.0
7	495.5
8	844.5
9	958.5
10	802.5
11	642.5
12	648.0
13	713.5
14	766.5
15	803.0
16	868.0
17	954.5
18	1007.0
19	1062.5
20	1177.5
21	1278.0
22	1401.5
23	1598.0
24	1820.5
25	2176.0
26	2735.0
27	3524.0
28	4560.0
29	5972.0
30	7766.5
31	9865.5
32	12590.0
33	15162.0
34	17572.5
35	20248.5
36	23583.0
37	27255.5
38	30710.0
39	34496.5
40	38352.5
41	42283.0
42	46237.5
43	51269.5
44	56892.0
45	62099.5
46	67260.0
47	72235.5
48	77765.0
49	82798.0
50	85107.0
51	86028.5
52	85977.0
53	85147.5
54	84541.0
55	82514.5
56	77450.0
57	70167.0
58	63828.5
59	57657.5
60	50065.5
61	43022.0
62	36535.0
63	28517.5
64	21017.5
65	16124.5
66	12463.0
67	9611.0
68	7431.0
69	5785.0
70	4607.5
71	3528.5
72	2706.0
73	2351.0
74	1907.0
75	1439.5
76	1036.5
77	584.5
78	314.0
79	203.0
80	127.0
81	65.0
82	34.0
83	18.5
84	11.5
85	9.0
86	8.0
87	2.5
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023901972906442307
2	0.002094779647980337
3	0.030723434837044945
4	0.16688411195576686
5	0.02293515153045138
6	0.06875174229268799
7	0.05339002487416551
8	0.045440604671573466
9	0.035074131029004106
10-11	0.06262854024474546
12-13	0.05717674193012997
14-15	0.057928714111456246
16-17	0.05086554683685588
18-19	0.03797459515697688
20-21	0.02400939750377463
22-23	0.003491299413300562
24-25	0.00496838762662003
26-27	0.0034375871146343994
28-29	0.00496838762662003
30-31	0.0026050464853088805
32-33	0.00886252927991681
34-35	0.004780394581288461
36-37	0.006928886527934962
38-39	0.002336484991978068
40-41	0.0035987240106328867
42-43	0.004028422399962187
44-45	0.004995243775953111
46-47	0.004699826133289217
48-49	0.0027661833813073682
50-51	0.0016113689599848748
52-53	9.668213759909248E-4
54-55	0.005290661418617004
56-57	8.325406293255187E-4
58-59	8.056844799924374E-4
60-61	2.6856149333081245E-5
62-63	9.93677525324006E-4
64-65	0.0
66-67	0.004135846997294511
68-69	9.399652266578436E-4
70-71	0.0
72-73	0.002443909589310393
74-75	0.0055323667626147365
76-77	0.001638225109317956
78-79	2.1484919466464996E-4
80-81	0.0
82-83	0.005908352853277874
84-85	0.0020410673493141745
86-87	0.0
88-89	9.399652266578436E-4
90-91	8.056844799924374E-4
92-93	0.0
94-95	0.003276450218635912
96-97	0.001745649706650281
98-99	8.056844799924374E-4
100-101	0.0
102-103	0.0016919374079841184
104-105	0.0
106-107	6.982598826601123E-4
108-109	0.002551334186642718
110-111	1.0742459733232498E-4
112-113	1.879930453315687E-4
114-115	0.0
116-117	0.001853074303982606
118-119	0.002981032575972018
120-121	0.0030347448746381807
122-123	0.0014770882133194685
124-125	0.10680690589766412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1861771.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.701973837131646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.2330776726293	47.30371130246734
2	11.14971481505044	13.31319964359241
3	3.8228077470750828	6.846875043007823
4	1.781314916368267	4.253920661308426
5	0.9759870254390355	2.9134175929070616
6	0.6395552318222079	2.290962583059007
7	0.4390249760293009	1.8347460342924071
8	0.3318115061614406	1.5847841487767658
9	0.23905585320445344	1.2844895664262919
>10	1.3103400890139265	13.682343900387817
>50	0.05932982496867713	2.395725329198727
>100	0.017438402044705304	1.7469233289364152
>500	1.8064673110140575E-4	0.07964697696723848
>1k	3.612934622028115E-4	0.46925388867224727
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3231	0.173544436990371	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2746	0.1474939721372822	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.371229866616249E-5	0.0
6	0.0	0.0	0.0	5.371229866616249E-5	1.6113689599848746E-4
7	0.0	0.0	0.0	5.371229866616249E-5	1.6113689599848746E-4
8	0.0	0.0	0.0	5.371229866616249E-5	1.6113689599848746E-4
9	0.0	0.0	0.0	5.371229866616249E-5	2.1484919466464996E-4
10-11	0.0	0.0	0.0	1.0742459733232498E-4	2.1484919466464996E-4
12-13	0.0	0.0	0.0	1.0742459733232498E-4	2.954176426638937E-4
14-15	0.0	0.0	0.0	1.0742459733232498E-4	3.4912994133005616E-4
16-17	0.0	0.0	0.0	1.6113689599848746E-4	4.296983893292999E-4
18-19	0.0	0.0	0.0	2.1484919466464996E-4	4.296983893292999E-4
20-21	0.0	0.0	0.0	2.1484919466464996E-4	4.834106879954624E-4
22-23	0.0	0.0	0.0	2.6856149333081246E-4	5.371229866616249E-4
24-25	0.0	0.0	0.0	6.176914346608686E-4	6.445475839939499E-4
26-27	0.0	0.0	0.0	9.668213759909249E-4	6.982598826601123E-4
28-29	0.0	0.0	0.0	0.003625580159965968	6.982598826601123E-4
30-31	0.0	0.0	0.0	0.011682424959890341	6.982598826601123E-4
32-33	0.0	0.0	0.0	0.02895092898106158	8.056844799924373E-4
34-35	0.0	0.0	0.0	0.05220835430350994	8.056844799924373E-4
36-37	0.0	0.0	0.0	0.08234095385522709	8.056844799924373E-4
38-39	0.0	0.0	0.0	0.13124600179076804	8.056844799924373E-4
40-41	0.0	0.0	0.0	0.18458231436626737	8.056844799924373E-4
42-43	0.0	0.0	0.0	0.2410876525630703	8.86252927991681E-4
44-45	0.0	0.0	0.0	0.30573040400779683	9.668213759909248E-4
46-47	0.0	2.6856149333081245E-5	0.0	0.3755563922738081	0.0010205336746570873
48-49	0.0	5.371229866616249E-5	0.0	0.44962565213444616	0.0011279582719894122
50-51	0.0	5.371229866616249E-5	0.0	0.5226743783204272	0.0011279582719894122
52-53	0.0	5.371229866616249E-5	0.0	0.5970390558237291	0.0013428074666540623
54-55	0.0	5.371229866616249E-5	0.0	0.6799708449642841	0.0015039443626525496
56-57	0.0	5.371229866616249E-5	0.0	0.7746656275127285	0.0015039443626525496
58-59	0.0	5.371229866616249E-5	0.0	0.8772292618157658	0.0015039443626525496
60-61	0.0	5.371229866616249E-5	0.0	0.9818608196174502	0.0015039443626525496
62-63	0.0	5.371229866616249E-5	0.0	1.091514477344421	0.0015039443626525496
64-65	0.0	5.371229866616249E-5	0.0	1.2076404670606644	0.0015308005119856309
66-67	0.0	5.371229866616249E-5	0.0	1.338725331955434	0.0015576566613187122
68-69	0.0	5.371229866616249E-5	0.0	1.480552656583436	0.0015576566613187122
70-71	0.0	5.371229866616249E-5	0.0	1.6309202366993578	0.0015845128106517932
72-73	0.0	5.371229866616249E-5	0.0	1.7773936751619828	0.0016919374079841184
74-75	0.0	5.371229866616249E-5	0.0	1.9321656637685303	0.0017187935573171997
76-77	0.0	5.371229866616249E-5	0.0	2.1161302867001366	0.0017187935573171997
78-79	0.0	5.371229866616249E-5	0.0	2.3232180542075263	0.0017187935573171997
80-81	0.0	5.371229866616249E-5	0.0	2.5292315757415924	0.0017993620053164433
82-83	0.0	5.371229866616249E-5	0.0	2.7293904567210467	0.0019336427519818496
84-85	0.0	5.371229866616249E-5	0.0	2.945985301092347	0.0019336427519818496
86-87	0.0	5.371229866616249E-5	0.0	3.1964188936233295	0.0019336427519818496
88-89	0.0	5.371229866616249E-5	0.0	3.4774416402446917	0.0019336427519818496
90-91	0.0	5.371229866616249E-5	0.0	3.7735575427912456	0.001960498901314931
92-93	0.0	5.371229866616249E-5	0.0	4.060058943876556	0.0021484919466464997
94-95	0.0	5.371229866616249E-5	0.0	4.355933141079112	0.0022559165439788244
96-97	0.0	5.371229866616249E-5	0.0	4.688439125971991	0.0024976218879765558
98-99	0.0	5.371229866616249E-5	0.0	5.072401492987054	0.002631902634641962
100-101	0.0	5.371229866616249E-5	0.0	5.476855101943258	0.002631902634641962
102-103	0.0	5.371229866616249E-5	0.0	5.885766831688752	0.002631902634641962
104-105	0.0	5.371229866616249E-5	0.0	6.306495267140804	0.002631902634641962
106-107	0.0	5.371229866616249E-5	0.0	6.766030838379156	0.002631902634641962
108-109	0.0	5.371229866616249E-5	0.0	7.2620102042625	0.0026856149333081246
110-111	0.0	5.371229866616249E-5	0.0	7.792848851980184	0.0029004641279727745
112-113	0.0	5.371229866616249E-5	0.0	8.322210411484548	0.0029004641279727745
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2925	0.0	58.334522	1
GTATCAA	5105	0.0	55.51155	1
TCAACGC	6660	0.0	42.357666	4
CAACGCA	6745	0.0	41.955532	5
TATCAAC	6890	0.0	41.122356	2
AACGCAG	6950	0.0	40.814693	6
ATCAACG	6935	0.0	40.59639	3
GTACATG	9875	0.0	37.155533	1
TACATGG	10265	0.0	35.67903	2
ACATGGG	10505	0.0	34.806175	3
ACGCAGA	8175	0.0	34.62397	7
CATGGGG	9015	0.0	33.211086	4
CGCAGAG	8720	0.0	32.523933	8
ATGGGGG	5650	0.0	30.05204	5
GCAGAGT	9780	0.0	28.755156	9
GTGGTAT	1565	0.0	25.160257	1
AGAGTAC	8905	0.0	24.85636	10-11
GTATAGG	485	0.0	24.602188	1
GAGTACT	4550	0.0	24.468319	12-13
CAGAGTA	9410	0.0	23.838997	10-11
>>END_MODULE