FastQCFastQC Report
Mon 15 Aug 2016
SRR2921588_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921588_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936328
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36950.045.260191
GGTATCA24000.038.188621
GTACATG100850.037.8864251
TCAACGC44550.037.135384
CAACGCA45350.036.6096275
TACATGG104400.036.025282
ACATGGG106650.035.0420573
ATCAACG48000.034.5902563
CATGGGG89700.034.4322784
AACGCAG48250.034.2841536
TATCAAC49100.033.5729182
ATGGGGG55850.031.8578435
ACGCAGA57800.028.5085037
GTCTCGT7250.026.2563579
CGCAGAG63300.026.0314588
GGTCTCG7650.024.1058648
GCAGAGT69450.023.1267179
GTATAGG5750.022.7708551
TGGGGGG44200.022.213026
GAGTACA70800.021.6875721
Produced by FastQC (version 0.11.5)