##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921588_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1936328
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.722005259439516	31.0	31.0	34.0	26.0	34.0
2	29.76060564119302	31.0	31.0	34.0	25.0	34.0
3	29.90787511206779	31.0	31.0	34.0	26.0	34.0
4	33.36891528707946	37.0	35.0	37.0	28.0	37.0
5	33.40270088538718	37.0	35.0	37.0	28.0	37.0
6	33.239173838316646	37.0	35.0	37.0	27.0	37.0
7	33.13711674881528	37.0	35.0	37.0	26.0	37.0
8	33.201313000689964	37.0	35.0	37.0	26.0	37.0
9	34.84073720981156	39.0	37.0	39.0	27.0	39.0
10-11	34.87457961667651	39.0	37.0	39.0	27.0	39.0
12-13	34.81014554352362	39.0	37.0	39.0	27.0	39.0
14-15	36.06162643932227	40.0	37.5	41.0	27.0	41.0
16-17	36.01287256084713	40.0	37.5	41.0	26.5	41.0
18-19	35.956589224552864	40.0	37.0	41.0	26.0	41.0
20-21	36.00273171694052	40.0	37.5	41.0	26.0	41.0
22-23	35.913772356749476	40.0	37.0	41.0	25.5	41.0
24-25	35.89994437925806	40.0	37.0	41.0	25.0	41.0
26-27	35.86828703608066	40.0	37.0	41.0	25.0	41.0
28-29	35.798274104387275	40.0	37.0	41.0	25.0	41.0
30-31	35.69880154601906	40.0	37.0	41.0	25.0	41.0
32-33	35.60632057172132	40.0	36.5	41.0	24.0	41.0
34-35	35.4766142409757	40.0	36.0	41.0	24.0	41.0
36-37	35.36384822199545	40.0	36.0	41.0	23.0	41.0
38-39	35.241428105155734	40.0	36.0	41.0	22.0	41.0
40-41	35.12398906590205	40.0	35.5	41.0	21.5	41.0
42-43	34.83369553092245	40.0	35.0	41.0	18.5	41.0
44-45	34.41395078726332	39.0	35.0	41.0	15.5	41.0
46-47	34.124017470180675	39.0	34.5	41.0	14.0	41.0
48-49	33.90736615903917	39.0	34.0	40.0	10.5	41.0
50-51	33.69017232617614	38.0	34.0	40.0	8.5	41.0
52-53	33.47645207836689	38.0	33.0	40.0	7.5	41.0
54-55	33.23057560495949	38.0	33.0	40.0	6.5	41.0
56-57	32.9747222577993	38.0	33.0	40.0	2.0	41.0
58-59	32.98170144727546	37.5	33.0	40.0	2.0	41.0
60-61	32.77158466953946	37.0	33.0	40.0	2.0	41.0
62-63	32.46673058490091	36.5	33.0	40.0	2.0	41.0
64-65	32.165994862440655	36.0	32.0	39.0	2.0	41.0
66-67	31.84226923331171	35.5	32.0	39.0	2.0	41.0
68-69	31.51374896195273	35.0	32.0	38.5	2.0	40.0
70-71	31.1574335546457	35.0	31.5	37.5	2.0	40.0
72-73	30.816884845955848	35.0	31.0	37.0	2.0	39.0
74-75	30.463086832396165	35.0	31.0	36.5	2.0	39.0
76-77	30.174472248503353	35.0	31.0	36.0	2.0	38.5
78-79	29.90929997397135	35.0	31.0	35.5	2.0	37.0
80-81	29.66254451725121	35.0	30.5	35.0	2.0	37.0
82-83	29.45088383786218	34.5	30.0	35.0	2.0	36.5
84-85	29.243798571316432	34.0	30.0	35.0	2.0	36.0
86-87	29.077162546841237	34.0	30.0	35.0	2.0	36.0
88-89	28.91460770076144	34.0	30.0	35.0	2.0	35.0
90-91	28.73497800992394	34.0	29.5	35.0	2.0	35.0
92-93	28.652706308022196	34.0	29.0	35.0	2.0	35.0
94-95	28.526521333162563	34.0	29.0	35.0	2.0	35.0
96-97	28.43596746005842	34.0	29.0	35.0	2.0	35.0
98-99	28.341153719824327	34.0	29.0	35.0	2.0	35.0
100-101	28.226766332976645	34.0	29.0	35.0	2.0	35.0
102-103	27.325126476506046	32.5	26.5	34.0	2.0	35.0
104-105	28.26076651269826	34.0	29.0	35.0	2.0	35.0
106-107	28.550536117847805	34.0	30.0	35.0	2.0	35.0
108-109	28.547678130977808	34.0	30.0	35.0	2.0	35.0
110-111	28.478144198710137	34.0	30.0	35.0	2.0	35.0
112-113	28.392447457248977	34.0	30.0	35.0	2.0	35.0
114-115	28.27102768745791	34.0	29.5	35.0	2.0	35.0
116-117	28.144715926227377	34.0	29.0	35.0	2.0	35.0
118-119	27.84484524316128	34.0	29.0	35.0	2.0	35.0
120-121	27.699830555567033	34.0	29.0	35.0	2.0	35.0
122-123	27.434651309075733	34.0	29.0	35.0	2.0	35.0
124-125	26.634159605190856	33.5	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	101800.0
3	17012.0
4	8130.0
5	6829.0
6	7006.0
7	7585.0
8	7887.0
9	8210.0
10	8581.0
11	9139.0
12	9409.0
13	9432.0
14	9454.0
15	8042.0
16	6797.0
17	6340.0
18	5862.0
19	5514.0
20	5573.0
21	5777.0
22	6654.0
23	8516.0
24	12015.0
25	15133.0
26	17402.0
27	20088.0
28	24097.0
29	28653.0
30	35035.0
31	43765.0
32	57575.0
33	79948.0
34	124335.0
35	200496.0
36	377630.0
37	512341.0
38	118215.0
39	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.57602582063585	17.038602842418907	11.61257061621559	25.772800720729656
2	16.76633407468393	19.02287374761506	37.90520766941479	26.305584508286216
3	20.11148138430294	23.04861092459718	28.619167938978613	28.220739752121265
4	13.769011215364543	14.57697818391049	33.86137243336326	37.79263816736171
5	14.7737392019405	36.873991475189	33.1029609565309	15.249308366339601
6	34.483252366710055	32.9110973036512	16.654226168261015	15.95142416137773
7	30.17612324234606	31.05852223489403	20.20159375437621	18.5637607683837
8	28.797330512831508	33.90291073514754	18.758335042754474	18.541423709266475
9	28.27496677055228	15.760392564662466	18.05718926139965	37.90745140338561
10-11	26.955053381408117	24.961220843292935	26.13896010888133	21.944765666417613
12-13	26.608329780142952	22.43181812899282	26.419595326265522	24.540256764598706
14-15	24.35672186838273	23.822484216703714	23.683054982851598	28.137738932061957
16-17	25.125779884362842	26.762022676414087	24.131061644994496	23.981135794228578
18-19	24.930443718699674	26.069513397947695	25.49583230645787	23.50421057689476
20-21	25.46491005565437	25.091168710805462	25.502648400033685	23.94127283350648
22-23	25.766491445741828	24.568567231181316	25.169970496857108	24.49497082621975
24-25	25.112066850065222	25.02780683740814	25.20229377348987	24.657832539036768
26-27	24.665909891774646	25.315231016127882	25.457862588004794	24.56099650409268
28-29	24.868148876097532	25.51630226284042	25.377727797278265	24.23782106378378
30-31	25.375523718531362	25.115604868590268	25.34631093712588	24.162560475752485
32-33	24.82427672683051	25.25827679088956	25.349948391136294	24.56749809114363
34-35	24.884115228846426	25.63376563052605	25.283896053368863	24.198223087258654
36-37	25.03368188956369	25.491941525605593	25.27028796369251	24.204088621138204
38-39	24.967799239785702	25.58753634934422	25.25921385979607	24.18545055107401
40-41	24.924992495374514	25.32058055059409	25.38916843582938	24.36525851820201
42-43	24.981203439609125	25.222853208096975	25.173521115556163	24.622422236737737
44-45	24.650259933826575	25.260314771870583	25.533097431343972	24.556327862958867
46-47	24.576640231756606	25.232975725065916	25.512081676846655	24.678302366330822
48-49	24.584599870642716	25.5688052635603	25.343230749838302	24.503364115958682
50-51	24.59633643886484	25.54889229671658	25.455439639476285	24.399331624942302
52-53	24.81006125823068	25.327257704287305	25.331571118514802	24.53110991896722
54-55	24.53470340344508	25.184233173645644	25.492390469981846	24.788672952927428
56-57	24.644456974392845	25.431054188525557	25.1364224678448	24.7880663692368
58-59	24.605987100360004	25.504178707510107	25.348466679459726	24.541367512670163
60-61	24.797040059921997	25.434511971485396	25.524007541906656	24.24444042668595
62-63	24.66702637056613	25.48235233952604	25.429868974970443	24.42075231493738
64-65	24.59150020830164	25.236860789676296	25.391414666450057	24.780224335572008
66-67	24.597067695313488	25.472569504175908	25.228090241131717	24.702272559378883
68-69	24.475857535633523	25.385172682319546	25.46206387108522	24.676905910961707
70-71	24.462572963745085	25.25350331898817	25.62725440340109	24.656669313865656
72-73	24.58505543528177	25.608527678381865	25.30680146973362	24.49961541660274
74-75	24.550675368570673	25.607862703292344	25.248795534179557	24.592666393957423
76-77	24.527479338842976	25.306250000000002	25.250748966942147	24.915521694214878
78-79	24.480223369317457	25.429749600618862	25.433753097867427	24.656273932196253
80-81	24.607761198964017	25.592116069832816	25.23885062434851	24.561272106854663
82-83	24.633862223404613	25.519819817492888	25.37768330134853	24.468634657753967
84-85	24.674005565897204	25.577250066629613	25.08914939289573	24.659594974577445
86-87	24.42863957449787	25.72821965612614	25.272117900495957	24.571022868880043
88-89	24.34746426802199	25.446194503662696	25.355021073201677	24.85132015511364
90-91	24.637954787416763	25.342312553948414	25.370206888144235	24.649525770490584
92-93	24.65068125773599	25.503476853557927	25.3455354834811	24.50030640522498
94-95	24.59718802506305	25.37843277927479	25.33300586701872	24.691373328643436
96-97	24.47523514857878	25.304388923610933	25.360380369936102	24.859995557874186
98-99	24.337056187824203	25.407093867425957	25.23415144199172	25.021698502758117
100-101	24.387537962522984	25.561793690473735	25.35881040431378	24.691857942689502
102-103	24.16320916988315	25.43732051337339	25.53459609973188	24.864874217011582
104-105	24.265862425238264	25.502773553513276	25.375103067319582	24.856260953928878
106-107	24.28482452171865	25.43420261652588	25.414112837420493	24.866860024334972
108-109	24.25905073785014	25.426775411538046	25.418795163298093	24.89537868731373
110-111	24.05518549237569	25.853558270232078	25.433325104606403	24.65793113278583
112-113	24.016209382291663	25.885587149832208	25.46312889728067	24.635074570595457
114-115	24.050732944932733	25.94243616996275	25.36920998554022	24.637620899564293
116-117	24.07086524705481	25.82431657723078	25.31822477933695	24.78659339637746
118-119	24.064662555558865	25.65515116283586	25.386164046407266	24.894022235198005
120-121	23.98469558103882	25.900123611820153	25.457450239593122	24.657730567547908
122-123	23.98432482806864	25.93228203844373	25.39564289921477	24.68775023427286
124-125	23.880738129880665	25.916348423124074	25.3824297444407	24.820483702554558
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	480.0
1	404.5
2	297.5
3	240.0
4	199.5
5	180.5
6	186.0
7	222.5
8	308.0
9	344.0
10	320.5
11	330.0
12	358.0
13	394.0
14	452.0
15	507.5
16	556.5
17	595.5
18	638.0
19	716.0
20	829.5
21	965.5
22	1080.5
23	1239.0
24	1498.0
25	1864.5
26	2379.5
27	3224.0
28	4358.5
29	5803.5
30	7649.5
31	9999.5
32	13036.5
33	15983.5
34	18923.0
35	22418.5
36	26522.0
37	30473.5
38	34002.5
39	37982.5
40	42041.5
41	45870.0
42	49653.5
43	54667.5
44	60102.5
45	65075.5
46	70080.5
47	75024.0
48	80600.5
49	84819.0
50	87282.5
51	88766.5
52	87772.5
53	86881.0
54	86527.0
55	84085.5
56	78900.5
57	72274.0
58	66246.0
59	59373.0
60	51233.0
61	44305.5
62	37822.0
63	29799.5
64	22467.5
65	17278.0
66	13244.5
67	10205.5
68	7919.5
69	6136.0
70	4982.5
71	3933.5
72	3014.5
73	2496.5
74	1998.0
75	1538.0
76	1110.5
77	692.0
78	480.0
79	386.0
80	300.5
81	211.0
82	156.0
83	120.0
84	117.0
85	102.0
86	75.0
87	72.5
88	64.5
89	50.0
90	44.5
91	36.5
92	32.0
93	31.0
94	25.5
95	21.5
96	17.5
97	13.5
98	9.5
99	8.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026441801182444293
2	0.011464999731450458
3	0.011361711445581534
4	0.012084729446663995
5	0.017145855454241226
6	0.09223643928094827
7	0.05933912023169628
8	0.05035303936109998
9	0.028146057899281526
10-11	0.05216058436380613
12-13	0.014176317235509687
14-15	0.02990195875905322
16-17	0.03638329869732814
18-19	0.04363930077961998
20-21	0.032587454191645215
22-23	0.03984345627393706
24-25	0.03372362533620337
26-27	0.04761589978557352
28-29	0.046815415570089364
30-31	0.027448861969666293
32-33	0.03170950376175937
34-35	0.046092397569006904
36-37	0.010251362372490611
38-39	0.022026226961547837
40-41	0.0441557422089646
42-43	0.02419528096479522
44-45	0.018307848670266608
46-47	0.0260286480389686
48-49	0.031193062332414753
50-51	0.030392578116930604
52-53	0.02615775839630476
54-55	0.024066170607459067
56-57	0.026932420540321682
58-59	0.01975388467243153
60-61	0.024685900322672608
62-63	0.024608434108270915
64-65	0.022387735962089067
66-67	0.030005247044922138
68-69	0.024556789965336452
70-71	0.04901029164480398
72-73	0.02411781475039353
74-75	0.010328828586892304
76-77	0.016939278882503378
78-79	0.02688077639738722
80-81	0.020141215744439993
82-83	0.026493445325378756
84-85	0.0130143240194843
86-87	0.01841113695613553
88-89	0.02360137332104891
90-91	0.023291508463442145
92-93	0.008598749798587843
94-95	0.012962679876549841
96-97	0.016422837453158763
98-99	0.006946137224685074
100-101	0.010741981730367996
102-103	0.003950776934486306
104-105	0.004828727364372152
106-107	0.0010328828586892303
108-109	0.015389954594469533
110-111	0.0011361711445581533
112-113	0.002169054003247384
114-115	0.013866452377902917
116-117	0.0060940088662664585
118-119	0.2778713110588702
120-121	0.0012652815018943072
122-123	9.037725013530765E-4
124-125	0.3135574138265831
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1936328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.65575171614493
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.9350917845111	54.45165794759552
2	9.554363285567156	12.545978073660958
3	2.956278536560994	5.822900688006507
4	1.351647443828644	3.549737159191016
5	0.7765334262070692	2.549194291516934
6	0.530332034860599	2.089160904475524
7	0.35251409691511576	1.6201204616449922
8	0.25771094649364623	1.3536164734015639
9	0.1944660888521753	1.1491035522199382
>10	1.0374666823571803	11.735103990953844
>50	0.04321468866471677	1.8961339637829417
>100	0.009909448452922942	1.0332720351563962
>500	4.715367287913364E-4	0.20402045839383745
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.5493242880338455E-4
7	0.0	0.0	0.0	0.0	1.5493242880338455E-4
8	0.0	0.0	0.0	0.0	2.0657657173784607E-4
9	0.0	0.0	0.0	0.0	2.582207146723076E-4
10-11	0.0	0.0	0.0	0.0	2.582207146723076E-4
12-13	0.0	0.0	0.0	0.0	3.098648576067691E-4
14-15	0.0	0.0	0.0	2.582207146723076E-5	3.615090005412306E-4
16-17	0.0	0.0	0.0	1.0328828586892303E-4	4.1315314347569214E-4
18-19	0.0	0.0	0.0	1.0328828586892303E-4	5.680855722790766E-4
20-21	0.0	0.0	0.0	1.5493242880338455E-4	6.197297152135382E-4
22-23	0.0	0.0	0.0	2.582207146723076E-4	6.713738581479997E-4
24-25	0.0	0.0	0.0	5.164414293446152E-4	6.713738581479997E-4
26-27	0.0	0.0	0.0	7.230180010824611E-4	7.230180010824612E-4
28-29	0.0	0.0	0.0	0.0033310472192727677	7.230180010824612E-4
30-31	0.0	0.0	0.0	0.014357071735780302	7.230180010824612E-4
32-33	0.0	0.0	0.0	0.0358410351965163	8.779504298858458E-4
34-35	0.0	0.0	0.0	0.0617922170210832	9.295945728203073E-4
36-37	0.0	0.0	0.0	0.09941497514883843	9.295945728203073E-4
38-39	0.0	0.0	0.0	0.15728223730690255	9.295945728203073E-4
40-41	0.0	0.0	0.0	0.22429051276436637	9.295945728203073E-4
42-43	0.0	0.0	0.0	0.28943959907618955	9.812387157547688E-4
44-45	0.0	0.0	0.0	0.3667250589776112	0.0010328828586892303
46-47	0.0	0.0	0.0	0.44894253452927396	0.0010845270016236917
48-49	0.0	0.0	0.0	0.5333032420127168	0.0011361711445581533
50-51	0.0	0.0	0.0	0.6173799067100203	0.0011361711445581533
52-53	0.0	0.0	0.0	0.7063369429146302	0.001161993216025384
54-55	0.0	0.0	0.0	0.8100642039984961	0.0011878152874926148
56-57	0.0	0.0	0.0	0.92117657752199	0.0011878152874926148
58-59	0.0	0.0	0.0	1.0370918563383889	0.0011878152874926148
60-61	0.0	0.0	0.0	1.1532395337979928	0.0012394594304270764
62-63	0.0	0.0	0.0	1.2838992154221804	0.0013427477162959993
64-65	0.0	0.0	0.0	1.4147654736181061	0.0013427477162959993
66-67	0.0	0.0	0.0	1.5634231390549536	0.0013427477162959993
68-69	0.0	0.0	0.0	1.728090488801484	0.0013427477162959993
70-71	0.0	0.0	0.0	1.8947203159795243	0.0013427477162959993
72-73	0.0	0.0	0.0	2.0518476208576235	0.0015751463595010764
74-75	0.0	0.0	0.0	2.223951727186716	0.0016526125739027685
76-77	0.0	0.0	0.0	2.430373366495759	0.0017042567168372301
78-79	0.0	0.0	0.0	2.6572460864068486	0.0017042567168372301
80-81	0.0	0.0	0.0	2.874409707446259	0.0017300787883044607
82-83	0.0	0.0	0.0	3.1012049611429466	0.001807545002706153
84-85	0.0	0.0	0.0	3.338458153783863	0.001807545002706153
86-87	0.0	0.0	0.0	3.6088927082601705	0.001807545002706153
88-89	0.0	0.0	0.0	3.909384153924335	0.0018591891456406146
90-91	0.0	0.0	0.0	4.2154015228824875	0.0018591891456406146
92-93	0.0	0.0	0.0	4.514369466330084	0.0019624774315095375
94-95	0.0	0.0	0.0	4.831309571518874	0.0020657657173784607
96-97	0.0	0.0	0.0	5.194109675633467	0.0020915877888456917
98-99	0.0	0.0	0.0	5.601427030957565	0.0021174098603129222
100-101	0.0	0.0	0.0	6.0287306695973	0.0021174098603129222
102-103	0.0	0.0	0.0	6.453529567304713	0.002246520217649076
104-105	0.0	0.0	0.0	6.899373453257919	0.0022723422891163065
106-107	7.746621440169228E-5	0.0	0.0	7.377520750616631	0.002298164360583537
108-109	1.5493242880338455E-4	0.0	0.0	7.894375333104722	0.0023756305749852297
110-111	1.5493242880338455E-4	0.0	0.0	8.44668361971732	0.0024272747179196912
112-113	1.5493242880338455E-4	0.0	0.0	8.981716940518343	0.0024272747179196912
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3695	0.0	45.26019	1
GGTATCA	2400	0.0	38.18862	1
GTACATG	10085	0.0	37.886425	1
TCAACGC	4455	0.0	37.13538	4
CAACGCA	4535	0.0	36.609627	5
TACATGG	10440	0.0	36.02528	2
ACATGGG	10665	0.0	35.042057	3
ATCAACG	4800	0.0	34.590256	3
CATGGGG	8970	0.0	34.432278	4
AACGCAG	4825	0.0	34.284153	6
TATCAAC	4910	0.0	33.572918	2
ATGGGGG	5585	0.0	31.857843	5
ACGCAGA	5780	0.0	28.508503	7
GTCTCGT	725	0.0	26.256357	9
CGCAGAG	6330	0.0	26.031458	8
GGTCTCG	765	0.0	24.105864	8
GCAGAGT	6945	0.0	23.126717	9
GTATAGG	575	0.0	22.770855	1
TGGGGGG	4420	0.0	22.21302	6
GAGTACA	7080	0.0	21.687572	1
>>END_MODULE