FastQCFastQC Report
Mon 15 Aug 2016
SRR2921588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936328
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25330.13081461405299102No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.10297842101131627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32600.048.14181
GTATCAA51050.044.0686531
TCAACGC62750.035.6505934
CAACGCA65250.034.4149365
ATCAACG65950.033.9277953
GTACATG108550.033.863751
AACGCAG67600.033.2314456
TATCAAC67600.033.0122642
TACATGG111400.032.5127682
CATGGGG92700.032.3052564
ACATGGG112400.032.0103343
ATGGGGG53450.031.0915665
GTATACG1055.8071496E-428.4161281
ACGCAGA80850.027.6365227
CGCAGAG84550.026.6371568
TGGGGGG37950.024.3374186
GCAGAGT95850.023.248199
GTGTAGC11100.022.5793021
GTACTTT38400.021.46599814-15
AGAGTAC87850.021.16033410-11
Produced by FastQC (version 0.11.5)