##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1936328
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.629438814085216	33.0	31.0	34.0	30.0	34.0
2	31.802955904164996	33.0	31.0	34.0	30.0	34.0
3	31.86682783082205	34.0	31.0	34.0	30.0	34.0
4	35.25876039596597	37.0	35.0	37.0	33.0	37.0
5	35.09151135551415	37.0	35.0	37.0	33.0	37.0
6	35.10806691841465	37.0	35.0	37.0	33.0	37.0
7	34.99433773616867	37.0	35.0	37.0	33.0	37.0
8	35.02696650567466	37.0	35.0	37.0	33.0	37.0
9	36.73579631136874	39.0	38.0	39.0	33.0	39.0
10-11	36.676704824802414	39.0	38.0	39.0	33.0	39.0
12-13	36.60319196954235	39.0	38.0	39.0	33.0	39.0
14-15	38.024412186365126	41.0	39.0	41.0	33.0	41.0
16-17	38.02570587214563	41.0	39.0	41.0	33.0	41.0
18-19	38.03574471886995	41.0	39.0	41.0	33.0	41.0
20-21	37.97185910651501	41.0	39.0	41.0	33.0	41.0
22-23	37.977520595684204	41.0	39.0	41.0	33.0	41.0
24-25	37.93628429687532	41.0	39.0	41.0	33.0	41.0
26-27	37.85970791105639	41.0	39.0	41.0	33.0	41.0
28-29	37.78775677467867	41.0	38.0	41.0	33.0	41.0
30-31	37.67737748976413	41.0	38.0	41.0	33.0	41.0
32-33	37.56139016736834	41.0	38.0	41.0	32.0	41.0
34-35	37.43394585008325	40.5	38.0	41.0	32.0	41.0
36-37	37.35846483653596	40.0	38.0	41.0	32.0	41.0
38-39	37.273788325118474	40.0	38.0	41.0	32.0	41.0
40-41	37.15627930805112	40.0	38.0	41.0	31.0	41.0
42-43	36.9967828281159	40.0	37.5	41.0	31.0	41.0
44-45	36.84981082750443	40.0	37.0	41.0	31.0	41.0
46-47	36.65408907994926	40.0	37.0	41.0	30.0	41.0
48-49	36.4698736474399	40.0	36.5	41.0	30.0	41.0
50-51	36.22371571345351	40.0	36.0	41.0	29.0	41.0
52-53	36.09528318549337	40.0	35.0	41.0	29.0	41.0
54-55	35.959943253415744	39.5	35.0	41.0	29.0	41.0
56-57	35.763658068261165	39.0	35.0	41.0	28.0	41.0
58-59	35.5657259513884	39.0	35.0	41.0	28.5	41.0
60-61	35.30912298949352	38.5	35.0	41.0	28.0	41.0
62-63	34.958213949289586	37.5	35.0	40.5	28.0	41.0
64-65	34.66782487264554	37.0	35.0	40.0	28.0	41.0
66-67	34.361792526885935	36.5	35.0	39.5	28.0	41.0
68-69	33.999334306997575	36.0	35.0	39.0	27.0	41.0
70-71	33.63186144083027	35.5	34.0	39.0	26.0	41.0
72-73	33.31814393016059	35.0	34.0	37.5	26.0	40.0
74-75	33.000476158997856	35.0	34.0	37.0	26.0	39.0
76-77	32.679010219343	35.0	34.0	36.5	26.0	39.0
78-79	32.42084786255221	35.0	34.0	36.0	26.0	38.0
80-81	32.17243566172673	35.0	34.0	36.0	25.5	37.0
82-83	31.954456321449673	35.0	34.0	35.0	25.0	37.0
84-85	31.74798174689412	35.0	34.0	35.0	24.5	36.0
86-87	31.623874932346173	35.0	33.0	35.0	24.0	36.0
88-89	31.52910044166071	35.0	33.0	35.0	24.0	36.0
90-91	31.426605151606545	35.0	33.0	35.0	24.0	35.5
92-93	31.346557246499557	35.0	33.0	35.0	24.0	35.0
94-95	31.281103717965138	35.0	33.0	35.0	23.5	35.0
96-97	31.214691415917137	35.0	33.0	35.0	23.0	35.0
98-99	31.137608142835305	35.0	33.0	35.0	22.0	35.0
100-101	31.06844165864461	35.0	33.0	35.0	20.0	35.0
102-103	30.37242114972257	34.0	31.5	35.0	19.5	35.0
104-105	30.89572143769031	35.0	33.0	35.0	20.0	35.0
106-107	30.994646568143413	35.0	33.0	35.0	20.0	35.0
108-109	30.97906191513008	35.0	33.0	35.0	20.0	35.0
110-111	30.912201083700694	35.0	33.0	35.0	19.0	35.0
112-113	30.87283843439748	35.0	33.0	35.0	19.0	35.0
114-115	30.745627548638453	35.0	33.0	35.0	18.0	35.0
116-117	30.930073572246023	35.0	33.0	35.0	19.0	35.0
118-119	30.911471610181746	35.0	33.0	35.0	17.5	35.0
120-121	30.826557793927474	35.0	33.0	35.0	11.5	35.0
122-123	30.68701867658785	35.0	33.0	35.0	2.0	35.0
124-125	30.034773292541345	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36874.0
3	10076.0
4	5259.0
5	4125.0
6	4226.0
7	4606.0
8	5008.0
9	5295.0
10	5543.0
11	5859.0
12	6442.0
13	7693.0
14	9755.0
15	8957.0
16	5768.0
17	4997.0
18	4562.0
19	4145.0
20	4004.0
21	4050.0
22	4610.0
23	5590.0
24	7411.0
25	9591.0
26	11795.0
27	13624.0
28	16435.0
29	19835.0
30	23845.0
31	30197.0
32	39349.0
33	53923.0
34	83930.0
35	149583.0
36	348731.0
37	716313.0
38	253933.0
39	389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.19304649137398	17.46771245827492	10.893719981445187	27.445521068905915
2	16.041931778743656	19.43151018856699	37.14752144309111	27.379036589598243
3	19.058787670578266	23.08860641784992	28.241277680626336	29.61132823094547
4	13.488650953849593	14.748328344836361	33.700392216910124	38.06262848440392
5	14.787192168101502	37.158591605462746	32.44925558325473	15.604960643181027
6	35.124280868146926	33.287716824842946	16.41868719547363	15.169315111536497
7	30.84140013579304	31.365092094344128	19.002913246435433	18.7905945234274
8	28.630484574396352	34.122331177591164	18.32549622801094	18.92168802000154
9	28.15583886889577	15.194821833932092	18.149360478512307	38.49997881865983
10-11	27.18364571151583	24.777311365143216	25.923737313969657	22.115305609371294
12-13	26.931002908208505	22.110239084916795	26.52267555182585	24.43608245504885
14-15	24.41674824892057	23.439569834796906	23.935186871806955	28.20849504447557
16-17	24.773836786322526	26.55343176970519	24.50628523950462	24.16644620446767
18-19	24.62949090195187	25.73549013732318	26.21627625827917	23.418742702445776
20-21	25.348939743370863	24.762369187146486	26.123853430197975	23.76483763928468
22-23	25.182645171019715	24.285610256431443	25.966963250954926	24.564781321593912
24-25	24.674946377450073	24.677993553415188	26.033573681489543	24.6134863876452
26-27	24.290276865360223	24.994422202114606	26.190756555978368	24.524544376546807
28-29	24.473343204785813	25.311521451816244	26.06466300627562	24.150472337122324
30-31	24.980226233068347	24.82190698389048	25.994207958311762	24.20365882472941
32-33	24.626542863156317	24.815269616103237	25.98590230782242	24.572285212918022
34-35	24.683936555418462	25.02782453217342	26.07821030543202	24.210028606976095
36-37	24.681178312894588	25.027037607840548	26.049534343701453	24.242249735563416
38-39	24.64425286258113	25.13795805042357	26.050456623716546	24.167332463278758
40-41	24.74858696370728	24.88756652012957	26.035038755205925	24.32880776095723
42-43	24.655919415239893	24.791026144908166	25.867412577011095	24.685641862840846
44-45	24.46499480043456	24.90115402721644	25.985690155480825	24.64816101686818
46-47	24.43539017775038	24.974247589318708	25.81356055192923	24.776801681001682
48-49	24.469369647602985	25.249834476589673	25.71283521718427	24.567960658623072
50-51	24.502780956099397	25.278541048378745	25.788716540794805	24.429961454727056
52-53	24.664490706550087	25.137400519544904	25.592828627647435	24.60528014625757
54-55	24.341563987920775	24.962826997180585	25.82530780382571	24.87030121107293
56-57	24.464691953530288	25.213459586895727	25.52650266098233	24.795345798591654
58-59	24.41615243804661	25.216435656501048	25.75227319735508	24.615138708097266
60-61	24.629071919984526	25.19656431731132	25.823809065159757	24.350554697544396
62-63	24.53101566276509	25.18666929709675	25.76056957763005	24.521745462508107
64-65	24.312060927725536	24.941990686490453	25.767729314315197	24.978219071468814
66-67	24.416526513211867	25.172799978102233	25.60311175530485	24.807561753381048
68-69	24.235184631442063	25.2006515462086	25.766932790447015	24.797231031902317
70-71	24.14306873628848	25.119039749463933	25.901035361777552	24.836856152470034
72-73	24.3684168394632	25.33519084225721	25.550345119649226	24.746047198630368
74-75	24.247535685179198	25.338052450163666	25.566330172220564	24.848081692436573
76-77	24.293730310385786	25.05618964003512	25.4764499302794	25.173630119299695
78-79	24.179477009553143	25.170658246596904	25.70556300953455	24.9443017343154
80-81	24.251314859879113	25.337055498861766	25.467172916985138	24.944456724273987
82-83	24.383789882350086	25.22553720269568	25.568036120039633	24.8226367949146
84-85	24.34214399702524	25.30677222936647	25.30315706841434	25.04792670519395
86-87	23.990563582203013	25.51360100148322	25.47739845728616	25.0184369590276
88-89	23.924692726928114	25.276802697092972	25.543082785942516	25.255421790036394
90-91	24.181860905895995	25.105031980850224	25.606990598120653	25.106116515133124
92-93	24.13532211484831	25.271131750405925	25.63620419680963	24.957341937936135
94-95	24.106589046196817	25.076526435771246	25.582812010170127	25.23407250786181
96-97	23.95056748798544	25.039553601424164	25.59873965583267	25.41113925475773
98-99	23.832259687153094	25.149271980352367	25.42056055459999	25.59790777789455
100-101	23.78613024239695	25.305939902743752	25.583114017873005	25.324815836986296
102-103	23.770002024475495	25.076485923697934	25.717965773969375	25.435546277857195
104-105	23.846734644130542	25.21543354225111	25.46601092376911	25.47182088984924
106-107	23.90795313579691	25.08361229921945	25.524397335561616	25.484037229422025
108-109	23.79302793875444	25.086132054862155	25.498006219170765	25.622833787212635
110-111	23.631933036103923	25.521799778653087	25.47769565780986	25.368571527433126
112-113	23.577245009547738	25.491104916665485	25.482531964587512	25.44911810919927
114-115	23.56511913270892	25.53831272387736	25.405096657177918	25.4914714862358
116-117	23.49328600574862	25.46383124648329	25.414587627947547	25.628295119820542
118-119	23.555658272563885	25.26426963042535	25.417243373892205	25.76282872311856
120-121	23.341035435177364	25.404515284041256	25.524978980182496	25.729470300598884
122-123	23.304011147009973	25.493259322916234	25.39580561729976	25.806923912774028
124-125	23.144289363523708	25.51150078660614	25.320161364119283	26.02404848575087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	44.0
2	46.0
3	72.0
4	73.5
5	68.5
6	159.0
7	387.0
8	655.0
9	745.0
10	607.0
11	494.5
12	536.0
13	607.0
14	639.0
15	688.0
16	734.0
17	798.5
18	877.0
19	915.0
20	980.0
21	1062.5
22	1154.5
23	1327.0
24	1572.0
25	1853.5
26	2370.0
27	3197.5
28	4209.0
29	5611.5
30	7434.0
31	9770.5
32	12742.0
33	15623.5
34	18569.0
35	21857.0
36	25876.0
37	30058.5
38	33402.5
39	37179.0
40	41484.5
41	45246.5
42	49116.5
43	54305.5
44	59774.5
45	64809.0
46	70145.5
47	75529.0
48	80844.0
49	85099.0
50	87871.5
51	89774.5
52	89520.5
53	88970.5
54	88370.0
55	85625.0
56	80368.5
57	73427.5
58	67249.5
59	60234.0
60	52057.0
61	44995.5
62	37865.5
63	29302.5
64	21568.0
65	16254.5
66	12323.0
67	9461.0
68	7293.5
69	5466.0
70	4258.5
71	3286.0
72	2494.0
73	2109.5
74	1657.5
75	1171.5
76	818.5
77	480.0
78	272.5
79	166.5
80	94.5
81	55.0
82	30.5
83	18.5
84	10.5
85	7.5
86	4.5
87	4.0
88	4.0
89	2.0
90	2.5
91	1.5
92	0.5
93	1.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02282671117703199
2	0.0020657657173784607
3	0.03269074247751414
4	0.16598427539135932
5	0.023239864320507682
6	0.07002945781912982
7	0.053193467222495364
8	0.04529191335352275
9	0.034188422622613526
10-11	0.06336736338058428
12-13	0.057634863514859046
14-15	0.060139604447180445
16-17	0.05164414293446152
18-19	0.038629818914977215
20-21	0.02579624939576353
22-23	0.0030986485760676912
24-25	0.005629211579856306
26-27	0.004131531434756921
28-29	0.005422635008118459
30-31	0.0026080292181903065
32-33	0.010096429943687226
34-35	0.004493040435298152
36-37	0.007023603439086766
38-39	0.0026338512896575375
40-41	0.0037183782912812292
42-43	0.004183175577691383
44-45	0.005603389508389074
46-47	0.005241880507847844
48-49	0.0023756305749852297
50-51	0.0017300787883044607
52-53	9.295945728203073E-4
54-55	0.005732499865725229
56-57	6.713738581479998E-4
58-59	6.971959296152306E-4
60-61	1.291103573361538E-4
62-63	8.263062869513842E-4
64-65	5.164414293446151E-5
66-67	0.002969538218731537
68-69	5.680855722790766E-4
70-71	0.0
72-73	0.0022206981461818454
74-75	0.004647972864101536
76-77	0.0014460360021649225
78-79	1.0328828586892302E-4
80-81	0.0
82-83	0.006068186794799229
84-85	0.0019624774315095375
86-87	0.0
88-89	0.001084527001623692
90-91	6.713738581479998E-4
92-93	0.0
94-95	0.0035634458624778445
96-97	0.0016267905024355375
98-99	6.971959296152306E-4
100-101	0.0
102-103	0.0012394594304270764
104-105	0.0
106-107	5.164414293446152E-4
108-109	0.0029437161472643065
110-111	5.164414293446151E-5
112-113	2.840427861395383E-4
114-115	0.0
116-117	0.0018850112171078454
118-119	0.002478918860854153
120-121	0.0030986485760676912
122-123	0.0012394594304270764
124-125	0.10685173173140088
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1936328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.936776678575114
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34133376805809	49.76083247651079
2	10.806032051879928	13.385815879576239
3	3.478723538605217	6.463827688112817
4	1.6554797238204761	4.101403118007121
5	0.9414230521941876	2.9154354671906977
6	0.5795378021974991	2.153682205889924
7	0.41131753559354395	1.783297764222744
8	0.3004942755418652	1.4889317469941374
9	0.22990959080356274	1.2815873083676563
>10	1.1922468544770435	12.85609359163366
>50	0.04993643179967079	2.074461827394603
>100	0.012979534327985846	1.2876505187258345
>500	3.347661149401689E-4	0.14560686122148725
>1k	2.510745862051238E-4	0.3013735461522676
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2533	0.13081461405299102	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1994	0.10297842101131627	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5493242880338455E-4
2	0.0	0.0	0.0	0.0	1.5493242880338455E-4
3	0.0	0.0	0.0	0.0	1.5493242880338455E-4
4	0.0	0.0	0.0	0.0	1.5493242880338455E-4
5	0.0	0.0	0.0	0.0	1.5493242880338455E-4
6	0.0	0.0	0.0	0.0	1.5493242880338455E-4
7	0.0	0.0	0.0	0.0	1.5493242880338455E-4
8	0.0	0.0	0.0	0.0	2.0657657173784607E-4
9	0.0	0.0	0.0	0.0	2.582207146723076E-4
10-11	0.0	0.0	0.0	0.0	2.582207146723076E-4
12-13	0.0	0.0	0.0	0.0	4.1315314347569214E-4
14-15	0.0	0.0	0.0	0.0	4.1315314347569214E-4
16-17	0.0	0.0	0.0	5.164414293446152E-5	5.164414293446152E-4
18-19	0.0	0.0	0.0	1.0328828586892303E-4	5.680855722790766E-4
20-21	0.0	0.0	0.0	1.5493242880338455E-4	6.45551786680769E-4
22-23	0.0	0.0	0.0	2.840427861395383E-4	7.230180010824611E-4
24-25	0.0	0.0	0.0	5.164414293446152E-4	8.263062869513843E-4
26-27	0.0	0.0	0.0	7.488400725496919E-4	8.779504298858457E-4
28-29	0.0	0.0	0.0	0.0032535810048710755	9.037725013530765E-4
30-31	0.0	0.0	0.0	0.01433124966431307	9.295945728203073E-4
32-33	0.0	0.0	0.0	0.03612507798265583	9.812387157547688E-4
34-35	0.0	0.0	0.0	0.06248941295069844	9.812387157547688E-4
36-37	0.0	0.0	0.0	0.10003470486405196	0.0010328828586892303
38-39	0.0	0.0	0.0	0.15785032287918163	0.0010328828586892303
40-41	0.0	0.0	0.0	0.22485859833664545	0.0010328828586892303
42-43	0.0	0.0	0.0	0.2905241260778133	0.0010587049301564611
44-45	0.0	0.0	0.0	0.36858424812325186	0.0011103490730909227
46-47	0.0	0.0	0.0	0.45144727546159535	0.0011878152874926148
48-49	0.0	0.0	0.0	0.5365568230175879	0.001394391859230461
50-51	0.0	0.0	0.0	0.6212273953586376	0.001394391859230461
52-53	0.0	0.0	0.0	0.711010737850199	0.0014202139306976917
54-55	0.0	0.0	0.0	0.8161582128647626	0.0014460360021649225
56-57	0.0	0.0	0.0	0.9279936043893391	0.0014976801450993838
58-59	0.0	0.0	0.0	1.0458971827087145	0.0015493242880338454
60-61	0.0	0.0	0.0	1.1642913803859676	0.0015493242880338454
62-63	0.0	0.0	0.0	1.2957515462256395	0.001600968430968307
64-65	0.0	0.0	0.0	1.4285544597816071	0.001600968430968307
66-67	0.0	0.0	0.0	1.580930503509736	0.001600968430968307
68-69	0.0	0.0	0.0	1.746992245115497	0.001600968430968307
70-71	0.0	0.0	0.0	1.9158427704397187	0.0016526125739027685
72-73	0.0	0.0	0.0	2.075991257679484	0.0017300787883044607
74-75	0.0	0.0	0.0	2.2522785395862686	0.0017817229312389223
76-77	0.0	0.0	0.0	2.4614373184708374	0.001807545002706153
78-79	0.0	0.0	0.0	2.693087121603365	0.001807545002706153
80-81	0.0	0.0	0.0	2.914278985791664	0.0018333670741733838
82-83	0.0	0.0	0.0	3.1460578992815265	0.0019108332885750762
84-85	0.0	0.0	0.0	3.3887079048590945	0.0019108332885750762
86-87	0.0	0.0	0.0	3.666708326275301	0.0019882995029767685
88-89	0.0	0.0	0.0	3.9745074181646913	0.002014121574443999
90-91	0.0	0.0	0.0	4.289071892778496	0.002014121574443999
92-93	0.0	0.0	0.0	4.59413384509236	0.002014121574443999
94-95	0.0	0.0	0.0	4.918123375791705	0.002014121574443999
96-97	0.0	0.0	0.0	5.286501047343219	0.0022723422891163065
98-99	0.0	0.0	0.0	5.702417152465904	0.002478918860854153
100-101	0.0	0.0	0.0	6.1378030994748825	0.0025047409323213834
102-103	0.0	0.0	0.0	6.569083337120571	0.0026080292181903065
104-105	0.0	0.0	0.0	7.0192653310802715	0.002659673361124768
106-107	0.0	0.0	0.0	7.50564986923703	0.0026854954325919987
108-109	0.0	0.0	0.0	8.030999913237839	0.0027113175040592297
110-111	0.0	0.0	0.0	8.5953154630827	0.002814605789928153
112-113	0.0	0.0	0.0	9.144886610119773	0.0028404278613953834
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3260	0.0	48.1418	1
GTATCAA	5105	0.0	44.068653	1
TCAACGC	6275	0.0	35.650593	4
CAACGCA	6525	0.0	34.414936	5
ATCAACG	6595	0.0	33.927795	3
GTACATG	10855	0.0	33.86375	1
AACGCAG	6760	0.0	33.231445	6
TATCAAC	6760	0.0	33.012264	2
TACATGG	11140	0.0	32.512768	2
CATGGGG	9270	0.0	32.305256	4
ACATGGG	11240	0.0	32.010334	3
ATGGGGG	5345	0.0	31.091566	5
GTATACG	105	5.8071496E-4	28.416128	1
ACGCAGA	8085	0.0	27.636522	7
CGCAGAG	8455	0.0	26.637156	8
TGGGGGG	3795	0.0	24.337418	6
GCAGAGT	9585	0.0	23.24819	9
GTGTAGC	1110	0.0	22.579302	1
GTACTTT	3840	0.0	21.465998	14-15
AGAGTAC	8785	0.0	21.160334	10-11
>>END_MODULE