FastQCFastQC Report
Mon 15 Aug 2016
SRR2921587_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921587_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399653
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG2750.095.207292
TACGGTC1450.069.761475
GTACGGT2250.060.824714
AGTACGG3800.046.97563
GGTATCA17700.040.3478431
GTACATG70950.040.2625431
GTATCAA25700.038.440351
TACATGG73550.038.267382
ACATGGG73100.037.361993
CATGGGG62850.036.0706864
ATGGGGG41950.034.8929635
CTAGTAC5150.033.5122151
TCAACGC28650.033.022294
GTACGAT1451.0977055E-732.8289264
CAACGCA29900.031.641765
ATCAACG30350.030.9765553
AACGCAG31600.030.312866
TATCAAC33250.028.4548112
AGTACGA3350.028.4190713
ACGGTCT4500.027.7648246
Produced by FastQC (version 0.11.5)