##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921587_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1399653
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.461838755748747	31.0	30.0	34.0	25.0	34.0
2	29.55251408742024	31.0	31.0	34.0	25.0	34.0
3	29.557526043955182	31.0	31.0	34.0	25.0	34.0
4	33.03361976146945	37.0	35.0	37.0	26.0	37.0
5	33.18058618814806	37.0	35.0	37.0	27.0	37.0
6	33.00773048748511	37.0	35.0	37.0	26.0	37.0
7	33.017322865024404	37.0	35.0	37.0	26.0	37.0
8	33.006997448653344	37.0	35.0	37.0	25.0	37.0
9	34.598596937955335	39.0	37.0	39.0	25.0	39.0
10-11	34.672530262858004	39.0	37.0	39.0	26.0	39.0
12-13	34.61439156705269	39.0	37.0	39.0	25.0	39.0
14-15	35.84141747990395	40.0	37.0	41.0	25.0	41.0
16-17	35.783102311787275	40.0	37.0	41.0	25.0	41.0
18-19	35.70761360137119	40.0	37.0	41.0	24.0	41.0
20-21	35.78402289710378	40.0	37.0	41.0	25.0	41.0
22-23	35.68335044471736	40.0	37.0	41.0	24.0	41.0
24-25	35.67137140419804	40.0	37.0	41.0	24.0	41.0
26-27	35.64522063682927	40.0	37.0	41.0	24.0	41.0
28-29	35.58786713564005	40.0	37.0	41.0	24.0	41.0
30-31	35.48209056101763	40.0	36.0	41.0	24.0	41.0
32-33	35.391310203314674	40.0	36.0	41.0	23.0	41.0
34-35	35.240467101488726	40.0	36.0	41.0	22.0	41.0
36-37	35.13173407980407	40.0	36.0	41.0	21.0	41.0
38-39	35.006825620350185	40.0	35.0	41.0	19.0	41.0
40-41	34.892894881802846	40.0	35.0	41.0	18.0	41.0
42-43	34.60673645539287	40.0	35.0	41.0	15.5	41.0
44-45	34.181919732962385	39.0	34.5	41.0	12.5	41.0
46-47	33.89879884514233	39.0	34.0	40.5	9.0	41.0
48-49	33.69489652078051	38.0	34.0	40.0	8.0	41.0
50-51	33.4811856938827	38.0	33.0	40.0	7.0	41.0
52-53	33.272860844795105	38.0	33.0	40.0	3.5	41.0
54-55	33.03519265132144	38.0	33.0	40.0	2.0	41.0
56-57	32.78455624358323	37.5	33.0	40.0	2.0	41.0
58-59	32.809684971918045	37.5	33.0	40.0	2.0	41.0
60-61	32.608935929119575	37.0	33.0	40.0	2.0	41.0
62-63	32.302921867062764	36.5	32.0	40.0	2.0	41.0
64-65	32.014897621053215	36.0	32.0	39.0	2.0	41.0
66-67	31.708517039580524	35.5	32.0	39.0	2.0	41.0
68-69	31.38538694947962	35.0	31.5	38.5	2.0	40.0
70-71	31.030718685274138	35.0	31.0	37.5	2.0	40.0
72-73	30.689601994208566	35.0	31.0	37.0	2.0	39.0
74-75	30.332678528178057	35.0	31.0	36.5	2.0	39.0
76-77	30.034183472617855	35.0	31.0	36.0	2.0	38.5
78-79	29.762953031930056	35.0	30.0	35.5	2.0	37.0
80-81	29.51195010477597	35.0	30.0	35.0	2.0	37.0
82-83	29.294416544672146	34.0	30.0	35.0	2.0	36.5
84-85	29.080477804141452	34.0	30.0	35.0	2.0	36.0
86-87	28.904825338851843	34.0	30.0	35.0	2.0	36.0
88-89	28.743917956807866	34.0	29.0	35.0	2.0	35.5
90-91	28.55826372679514	34.0	29.0	35.0	2.0	35.0
92-93	28.4811846221885	34.0	29.0	35.0	2.0	35.0
94-95	28.35396737619967	34.0	29.0	35.0	2.0	35.0
96-97	28.26628135687917	34.0	29.0	35.0	2.0	35.0
98-99	28.171019174038136	34.0	29.0	35.0	2.0	35.0
100-101	28.063796526710547	34.0	29.0	35.0	2.0	35.0
102-103	27.164516133641698	32.5	26.0	34.0	2.0	35.0
104-105	28.096110964646236	34.0	29.0	35.0	2.0	35.0
106-107	28.387105232511203	34.0	29.0	35.0	2.0	35.0
108-109	28.396964461905917	34.0	30.0	35.0	2.0	35.0
110-111	28.342996085458324	34.0	29.0	35.0	2.0	35.0
112-113	28.26354532159042	34.0	29.0	35.0	2.0	35.0
114-115	28.138408948503667	34.0	29.0	35.0	2.0	35.0
116-117	28.016105777646317	34.0	29.0	35.0	2.0	35.0
118-119	27.73971334323579	34.0	29.0	35.0	2.0	35.0
120-121	27.60429799385991	34.0	29.0	35.0	2.0	35.0
122-123	27.341327100359877	34.0	28.5	35.0	2.0	35.0
124-125	26.533359697010617	33.5	25.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78629.0
3	12223.0
4	5885.0
5	5030.0
6	5174.0
7	5580.0
8	5807.0
9	6136.0
10	6531.0
11	6503.0
12	6642.0
13	6738.0
14	6725.0
15	6181.0
16	5211.0
17	4882.0
18	4582.0
19	4308.0
20	4235.0
21	4330.0
22	4923.0
23	6494.0
24	9000.0
25	11493.0
26	12887.0
27	14735.0
28	17114.0
29	20916.0
30	25555.0
31	31964.0
32	42179.0
33	58562.0
34	90838.0
35	143253.0
36	266348.0
37	364752.0
38	87245.0
39	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.230977299607304	16.72518339330315	11.00252623605121	26.04131307103833
2	16.963723017341785	19.212795907138926	38.379718615801245	25.443762459718045
3	19.93257750978362	24.592631970807403	27.756687409300103	27.71810311010887
4	14.2440263148681	14.864099154491598	34.23569484527832	36.65617968536198
5	14.580932094085467	37.309416335405814	32.84144996859483	15.268201601913894
6	33.905127915408634	34.57162962528587	16.943303016563853	14.579939442741638
7	30.197301024358264	31.52033811186087	19.417769316720364	18.864591547060503
8	28.998581093880116	33.9566751133874	18.76594482349451	18.27879896923797
9	28.621014447182315	15.884182422118426	17.756756215947355	37.738046914751905
10-11	26.891374416255704	25.201241222837528	26.3261262253001	21.581258135606667
12-13	26.49044952591506	22.70920277487691	26.485233323246295	24.315114375961738
14-15	24.24161863301792	24.02350638310645	23.76662418051576	27.968250803359872
16-17	25.001581265805957	26.839994839891922	24.226600214194512	23.931823680107605
18-19	24.841886547142416	26.191278591668993	25.543886333429704	23.422948527758887
20-21	25.366898634101894	25.34217132239305	25.30168570943622	23.98924433406884
22-23	25.381689687347343	24.902288226236035	25.15083367252967	24.565188413886954
24-25	24.854185888781764	25.274636527819567	25.178296656763482	24.692880926635187
26-27	24.44835991165181	25.527372927999085	25.33573741431441	24.688529746034696
28-29	24.73993839362393	25.988648673182784	25.168659873692278	24.10275305950101
30-31	25.439446233249086	25.37402017238034	25.266179729069645	23.920353865300925
32-33	24.696328064671857	25.50040253750495	25.371833523494935	24.431435874328255
34-35	24.754204874659674	26.003748308650778	25.28439326934142	23.95765354734813
36-37	24.859871448644657	25.917413739082406	25.137895640536374	24.084819171736566
38-39	24.94167896687537	25.91083179424404	25.112434805320838	24.035054433559758
40-41	24.82711791719529	25.590229258617303	25.296320783374732	24.28633204081268
42-43	24.849360137891214	25.543068136667802	25.0936496865719	24.513922038869087
44-45	24.408310291469014	25.631794878757336	25.558372941282816	24.401521888490834
46-47	24.289954581819366	25.533943220663897	25.507287597381463	24.66881460013528
48-49	24.420638508087208	25.70179967876279	25.431052811182912	24.446509001967087
50-51	24.432766120297856	25.704537763838747	25.502110567057002	24.360585548806394
52-53	24.58544554487286	25.652547856844492	25.329923065296413	24.432083532986233
54-55	24.32417560625621	25.26593663542232	25.75441464998126	24.655473108340214
56-57	24.39313645233296	25.633966278271615	25.31948884643132	24.653408422964105
58-59	24.402874448839476	25.7753583860898	25.50571356195203	24.31605360311869
60-61	24.772555602482004	25.672435761384012	25.499998928076046	24.055009708057938
62-63	24.623744549695807	25.717503538253695	25.487396456279477	24.171355455771025
64-65	24.462570080718553	25.327210095125054	25.516259655694697	24.693960168461704
66-67	24.489762372699662	25.52235125960336	25.385099160264428	24.602787207432552
68-69	24.312001180534192	25.562817174771585	25.543379807934517	24.581801836759706
70-71	24.236447110439528	25.401835143053813	25.77216606779465	24.589551678712013
72-73	24.35144517923424	25.849792259504824	25.304142947185177	24.49461961407576
74-75	24.35082647418293	25.74118659163669	25.30411113163388	24.603875802546497
76-77	24.310605462420714	25.36149423213448	25.474539894507387	24.853360410937416
78-79	24.23978549412603	25.42333394554026	25.697362055128636	24.639518505205068
80-81	24.395153654588913	25.64268385492406	25.346915296143717	24.615247194343308
82-83	24.526849020110188	25.695205977493995	25.34996575111188	24.427979251283933
84-85	24.467037611585372	25.760545456234386	25.207807992774505	24.56460893940574
86-87	24.22772018281755	25.8856832934599	25.32023798294732	24.566358540775234
88-89	24.181181456942284	25.616385053473103	25.39660420545704	24.80582928412758
90-91	24.46727851298905	25.455008000022865	25.4518637302045	24.625849756783584
92-93	24.32604591093971	25.62442057578913	25.558471159634383	24.491062353636774
94-95	24.382422293676314	25.440693104680246	25.45530546623794	24.721579135405502
96-97	24.197884797531586	25.476431473864018	25.5026919686832	24.822991759921198
98-99	24.117767263467314	25.4955793691509	25.317060762774958	25.069592604606832
100-101	24.016881429855534	25.634750622710833	25.487522203946195	24.860845743487438
102-103	23.88210383719115	25.473910665477284	25.733769789965667	24.9102157073659
104-105	24.04372267957708	25.54058534117751	25.5488734590174	24.86681852022801
106-107	23.91927644303075	25.57048557451518	25.644290214419268	24.865947768034804
108-109	23.997854868491007	25.475115902841694	25.69441665570997	24.832612572957338
110-111	23.794485945150956	25.848261858483045	25.661071067126834	24.696181129239168
112-113	23.76926987306971	25.988683409466738	25.55220982169172	24.689836895771837
114-115	23.822843539685028	26.01171806195731	25.54496776800538	24.620470630352273
116-117	23.79286064135692	25.764345648103593	25.572752387413505	24.87004132312598
118-119	23.758535099179277	25.63576436266304	25.646045348820962	24.95965518933672
120-121	23.680772601152228	25.877201599988	25.695261373407575	24.7467644254522
122-123	23.63706966181673	25.91122506100674	25.601395501201917	24.850309775974615
124-125	23.575689055559838	25.757947677824983	25.676208837183466	24.990154429431715
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	269.5
2	199.0
3	153.5
4	133.5
5	141.0
6	156.0
7	163.0
8	183.5
9	224.5
10	246.0
11	225.5
12	241.5
13	286.5
14	326.5
15	358.5
16	413.0
17	457.0
18	478.0
19	545.0
20	609.5
21	670.5
22	802.0
23	1010.0
24	1248.5
25	1628.5
26	2223.5
27	3045.5
28	4103.5
29	5336.5
30	6850.0
31	8634.0
32	10870.5
33	12910.0
34	14976.5
35	17226.0
36	19798.5
37	22670.5
38	25075.0
39	27833.0
40	30629.5
41	32982.5
42	35478.0
43	39337.5
44	43409.0
45	47280.5
46	50695.5
47	53643.5
48	58106.5
49	62305.5
50	64802.0
51	65277.0
52	63873.5
53	62556.5
54	61557.0
55	58909.5
56	54828.5
57	50297.0
58	46164.5
59	41698.5
60	35593.5
61	29771.5
62	24884.5
63	19645.0
64	14934.0
65	11745.0
66	9275.0
67	7376.0
68	5919.0
69	4861.5
70	4041.5
71	3156.0
72	2493.0
73	2144.0
74	1803.0
75	1452.5
76	1130.5
77	783.0
78	504.0
79	344.0
80	254.5
81	192.0
82	141.0
83	105.0
84	84.0
85	62.0
86	42.5
87	35.0
88	31.5
89	25.0
90	17.5
91	14.0
92	15.5
93	13.5
94	10.0
95	8.5
96	10.0
97	8.5
98	6.0
99	5.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02414884260598877
2	0.010216818025610634
3	0.008573553587925008
4	0.010002479185912507
5	0.014432148539673763
6	0.09338028782848319
7	0.0590146271968838
8	0.04915504057077004
9	0.0249347516848819
10-11	0.05072685872855629
12-13	0.011967251883145322
14-15	0.026685185542416584
16-17	0.03268667305396409
18-19	0.04133167292178847
20-21	0.027899772300705963
22-23	0.03661621844842972
24-25	0.031436363155725025
26-27	0.04593995797529816
28-29	0.0435465075986691
30-31	0.025970722743422832
32-33	0.028900020219297214
34-35	0.044439586097411284
36-37	0.009609524646465945
38-39	0.020469359191170954
40-41	0.04129594978183878
42-43	0.02064797489091939
44-45	0.014825103079120326
46-47	0.02311287154744783
48-49	0.026828078102215335
50-51	0.027471094621309707
52-53	0.024970474824831584
54-55	0.021898284789158454
56-57	0.024827582265032833
58-59	0.015682458437912824
60-61	0.021433883969812516
62-63	0.021755392229359707
64-65	0.020326466631372202
66-67	0.028792850799448152
68-69	0.020469359191170954
70-71	0.04529694145620378
72-73	0.022219793048705642
74-75	0.00882361556757282
76-77	0.015611012158013452
78-79	0.02457752028538502
80-81	0.017718677415045014
82-83	0.02411311946603908
84-85	0.011717189903497509
86-87	0.01607541297735939
88-89	0.021755392229359707
90-91	0.020147850931623766
92-93	0.006823119730390318
94-95	0.010931280824604383
96-97	0.015182334478617202
98-99	0.0056442561120506295
100-101	0.009073677547220632
102-103	0.0034651445751196904
104-105	0.004215330514063129
106-107	8.573553587925007E-4
108-109	0.014646487379371888
110-111	8.216322188428132E-4
112-113	0.0018933264173334392
114-115	0.013539070040931574
116-117	0.005251301572604067
118-119	0.27667571891032994
120-121	0.0012503098982390634
122-123	8.930784987421882E-4
124-125	0.31118427210172805
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1399653.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.00030417809965
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.61457206850666	59.93046086873301
2	8.348379273486715	11.687781770764254
3	2.4039656517555037	5.048349805697664
4	1.1319350791749399	3.169431994084284
5	0.6337537640438582	2.218147812854283
6	0.40616265155685993	1.7058905492858216
7	0.28658595312260043	1.4042772724226846
8	0.20969624428872793	1.1743040708172838
9	0.15256217588554888	0.9611458846255045
>10	0.761156918046459	9.257445618623358
>50	0.03779711664437055	1.7561257964988535
>100	0.013127818356352506	1.541820836143037
>500	3.052851313925488E-4	0.14481771944996846
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	3.572313994968753E-5	0.0
16-17	0.0	0.0	0.0	7.144627989937506E-5	0.0
18-19	0.0	0.0	0.0	7.144627989937506E-5	0.0
20-21	0.0	0.0	0.0	1.0716941984906258E-4	0.0
22-23	0.0	0.0	0.0	1.7861569974843766E-4	1.4289255979875012E-4
24-25	0.0	0.0	0.0	3.2150825954718775E-4	2.8578511959750024E-4
26-27	0.0	0.0	0.0	5.715702391950005E-4	2.8578511959750024E-4
28-29	0.0	0.0	0.0	0.002286280956780002	2.8578511959750024E-4
30-31	0.0	0.0	0.0	0.010323987445459697	2.8578511959750024E-4
32-33	0.0	0.0	0.0	0.02768543346100784	2.8578511959750024E-4
34-35	0.0	0.0	0.0	0.0486906397514241	2.8578511959750024E-4
36-37	0.0	0.0	0.0	0.07598311867298538	2.8578511959750024E-4
38-39	0.0	0.0	0.0	0.1254239443633529	2.8578511959750024E-4
40-41	0.0	0.0	0.0	0.19011855081223705	2.8578511959750024E-4
42-43	0.0	0.0	0.0	0.2524197068844921	2.8578511959750024E-4
44-45	0.0	0.0	0.0	0.3143636315572502	2.8578511959750024E-4
46-47	0.0	0.0	0.0	0.3839165850392919	2.8578511959750024E-4
48-49	0.0	0.0	0.0	0.45882800951378666	3.572313994968753E-4
50-51	0.0	0.0	0.0	0.5413841859375145	3.572313994968753E-4
52-53	0.0	0.0	0.0	0.6310135440712805	4.644008193459379E-4
54-55	0.0	0.0	0.0	0.7297165797522671	5.001239592956255E-4
56-57	0.0	0.0	0.0	0.8280981071737066	5.001239592956255E-4
58-59	0.0	0.0	0.0	0.9378753162390965	5.001239592956255E-4
60-61	0.0	0.0	0.0	1.0563332483122603	5.001239592956255E-4
62-63	0.0	0.0	0.0	1.2039412625843693	5.001239592956255E-4
64-65	0.0	0.0	0.0	1.3306869631258604	5.001239592956255E-4
66-67	0.0	0.0	0.0	1.47022154776934	5.001239592956255E-4
68-69	0.0	0.0	0.0	1.6312614626625312	5.001239592956255E-4
70-71	0.0	0.0	0.0	1.7995531749655094	5.001239592956255E-4
72-73	0.0	0.0	0.0	1.9489830693750523	5.715702391950005E-4
74-75	0.0	0.0	0.0	2.1081296578509106	5.715702391950005E-4
76-77	0.0	0.0	0.0	2.3020348614978143	6.072933791446879E-4
78-79	0.0	0.0	0.0	2.5115510773027316	7.144627989937506E-4
80-81	0.0	0.0	0.0	2.7167447931737367	7.144627989937506E-4
82-83	0.0	0.0	0.0	2.9301548312331698	7.144627989937506E-4
84-85	0.0	0.0	0.0	3.1501736501832953	7.501859389434382E-4
86-87	0.0	0.0	0.0	3.40230757194819	8.573553587925007E-4
88-89	0.0	0.0	0.0	3.6871638899069987	9.288016386918758E-4
90-91	0.0	0.0	0.0	3.9818440713519707	9.288016386918758E-4
92-93	7.144627989937506E-5	0.0	0.0	4.272416091702729	9.288016386918758E-4
94-95	1.7861569974843766E-4	0.0	0.0	4.5680250747863935	9.288016386918758E-4
96-97	2.1433883969812517E-4	0.0	0.0	4.904608499392349	9.645247786415634E-4
98-99	2.1433883969812517E-4	0.0	0.0	5.285667233235666	0.001000247918591251
100-101	2.1433883969812517E-4	0.0	0.0	5.698448115354306	0.001000247918591251
102-103	2.1433883969812517E-4	0.0	0.0	6.113801063549323	0.001143140478390001
104-105	2.1433883969812517E-4	0.0	0.0	6.530868722461925	0.0011788636183396885
106-107	2.1433883969812517E-4	0.0	0.0	6.992483136891787	0.001214586758289376
108-109	2.1433883969812517E-4	0.0	0.0	7.4804612286045185	0.0012503098982390637
110-111	2.1433883969812517E-4	0.0	0.0	8.00387667514734	0.001286033038188751
112-113	2.1433883969812517E-4	0.0	0.0	8.499070841129909	0.001286033038188751
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	275	0.0	95.20729	2
TACGGTC	145	0.0	69.76147	5
GTACGGT	225	0.0	60.82471	4
AGTACGG	380	0.0	46.9756	3
GGTATCA	1770	0.0	40.347843	1
GTACATG	7095	0.0	40.262543	1
GTATCAA	2570	0.0	38.44035	1
TACATGG	7355	0.0	38.26738	2
ACATGGG	7310	0.0	37.36199	3
CATGGGG	6285	0.0	36.070686	4
ATGGGGG	4195	0.0	34.892963	5
CTAGTAC	515	0.0	33.512215	1
TCAACGC	2865	0.0	33.02229	4
GTACGAT	145	1.0977055E-7	32.828926	4
CAACGCA	2990	0.0	31.64176	5
ATCAACG	3035	0.0	30.976555	3
AACGCAG	3160	0.0	30.31286	6
TATCAAC	3325	0.0	28.454811	2
AGTACGA	335	0.0	28.419071	3
ACGGTCT	450	0.0	27.764824	6
>>END_MODULE