FastQCFastQC Report
Mon 15 Aug 2016
SRR2921587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1399653
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.14110640280126574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.10581194053097447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36300.049.9367941
TCAACGC44200.040.1847344
CAACGCA44950.039.7552455
GTACATG74700.039.3531951
TATCAAC46450.038.6286962
AACGCAG46700.038.5357556
ATCAACG46400.038.4120033
TACATGG75700.038.1134952
GGTATCA21700.037.370841
ACATGGG76550.037.143853
ATGGGGG40000.035.2932175
CATGGGG64000.035.109814
ACGCAGA57400.031.0397077
CGCAGAG59850.029.7690738
TATCACG1254.821481E-528.613472
GCAGAGT65500.027.1102339
TGGGGGG30200.026.6379436
AGAGTAC61200.023.36781510-11
GAGTACT30500.022.54670712-13
GGATCGT2505.203656E-721.4524236
Produced by FastQC (version 0.11.5)