##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1399653
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.646980358703193	33.0	31.0	34.0	30.0	34.0
2	31.82713644024626	33.0	31.0	34.0	30.0	34.0
3	31.90005022673477	34.0	31.0	34.0	30.0	34.0
4	35.29339700625798	37.0	35.0	37.0	33.0	37.0
5	35.1490362254073	37.0	35.0	37.0	33.0	37.0
6	35.17014502880357	37.0	35.0	37.0	33.0	37.0
7	35.05220150994568	37.0	35.0	37.0	33.0	37.0
8	35.082939128483986	37.0	35.0	37.0	33.0	37.0
9	36.78827466522059	39.0	38.0	39.0	33.0	39.0
10-11	36.72996021156672	39.0	38.0	39.0	33.0	39.0
12-13	36.65967136140172	39.0	38.0	39.0	33.0	39.0
14-15	38.072425451165394	41.0	39.0	41.0	33.0	41.0
16-17	38.07708696369743	41.0	39.0	41.0	33.0	41.0
18-19	38.083032723110655	41.0	39.0	41.0	33.5	41.0
20-21	38.01858567802162	41.0	39.0	41.0	33.0	41.0
22-23	38.02615112460017	41.0	39.0	41.0	33.0	41.0
24-25	37.98358878950711	41.0	39.0	41.0	33.0	41.0
26-27	37.90786216297897	41.0	39.0	41.0	33.0	41.0
28-29	37.83479405252588	41.0	38.0	41.0	33.0	41.0
30-31	37.71862025802109	41.0	38.0	41.0	33.0	41.0
32-33	37.599472154884104	41.0	38.0	41.0	33.0	41.0
34-35	37.463330911304446	40.0	38.0	41.0	32.0	41.0
36-37	37.38627466950737	40.0	38.0	41.0	32.0	41.0
38-39	37.300359803465575	40.0	38.0	41.0	32.0	41.0
40-41	37.17913261358351	40.0	38.0	41.0	31.0	41.0
42-43	37.023150023613	40.0	37.5	41.0	31.0	41.0
44-45	36.87544519963163	40.0	37.0	41.0	31.0	41.0
46-47	36.67842243756131	40.0	37.0	41.0	30.0	41.0
48-49	36.49814168225981	40.0	36.5	41.0	30.0	41.0
50-51	36.25909850512949	40.0	36.0	41.0	29.0	41.0
52-53	36.13924308382149	40.0	36.0	41.0	29.0	41.0
54-55	36.014683282213525	40.0	35.0	41.0	29.0	41.0
56-57	35.82176225107223	39.0	35.0	41.0	29.0	41.0
58-59	35.63856541585665	39.0	35.0	41.0	28.5	41.0
60-61	35.38818157071788	39.0	35.0	41.0	28.0	41.0
62-63	35.04076974793038	38.0	35.0	41.0	28.0	41.0
64-65	34.757050140284775	37.0	35.0	40.0	28.0	41.0
66-67	34.46063274254404	37.0	35.0	39.5	28.0	41.0
68-69	34.095297905980985	36.0	35.0	39.0	27.5	41.0
70-71	33.72737957193676	36.0	34.5	39.0	26.0	41.0
72-73	33.40804077867871	35.0	34.0	37.5	26.0	40.0
74-75	33.08900456041604	35.0	34.0	37.0	26.0	39.0
76-77	32.76083893650784	35.0	34.0	37.0	26.0	39.0
78-79	32.49809166986389	35.0	34.0	36.0	26.0	38.5
80-81	32.24668578569117	35.0	34.0	36.0	26.0	37.0
82-83	32.01941695548825	35.0	34.0	35.5	25.0	37.0
84-85	31.804862705256234	35.0	34.0	35.0	24.5	36.5
86-87	31.67401134424032	35.0	33.5	35.0	25.0	36.0
88-89	31.5735614470158	35.0	33.0	35.0	24.5	36.0
90-91	31.46585510837329	35.0	33.0	35.0	24.0	36.0
92-93	31.383443610666358	35.0	33.0	35.0	24.0	35.0
94-95	31.31854716847676	35.0	33.0	35.0	24.0	35.0
96-97	31.249484336474826	35.0	33.0	35.0	23.0	35.0
98-99	31.17557566053872	35.0	33.0	35.0	23.0	35.0
100-101	31.10550257813901	35.0	33.0	35.0	21.5	35.0
102-103	30.421276202030075	34.0	31.5	35.0	20.0	35.0
104-105	30.933079841932248	35.0	33.0	35.0	20.0	35.0
106-107	31.031629625342852	35.0	33.0	35.0	20.0	35.0
108-109	31.018747146614196	35.0	33.0	35.0	20.0	35.0
110-111	30.953885713101748	35.0	33.0	35.0	19.5	35.0
112-113	30.91391044780385	35.0	33.0	35.0	19.0	35.0
114-115	30.78981719040362	35.0	33.0	35.0	18.0	35.0
116-117	30.975012378067994	35.0	33.0	35.0	19.0	35.0
118-119	30.957117942804395	35.0	33.0	35.0	18.5	35.0
120-121	30.872801687275345	35.0	33.0	35.0	16.0	35.0
122-123	30.735573388547017	35.0	33.0	35.0	2.0	35.0
124-125	30.084972132378525	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	24955.0
3	6777.0
4	3688.0
5	2804.0
6	2956.0
7	3273.0
8	3585.0
9	3689.0
10	4104.0
11	4383.0
12	4716.0
13	5740.0
14	7549.0
15	6885.0
16	4267.0
17	3796.0
18	3389.0
19	3007.0
20	2802.0
21	2890.0
22	3279.0
23	4107.0
24	5373.0
25	7024.0
26	8475.0
27	9768.0
28	11779.0
29	14300.0
30	17613.0
31	21908.0
32	28675.0
33	38617.0
34	61521.0
35	108391.0
36	244357.0
37	515406.0
38	193364.0
39	441.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.534915795897774	17.309184771656312	10.368760085125967	27.787139347319943
2	16.27576322447291	19.754990815370732	36.63591786633667	27.333328093819688
3	18.938979758543283	23.689500927992498	28.26564383812266	29.10587547534156
4	13.184835439124784	14.8408920464629	34.04233241926527	37.93194009514704
5	14.466838936879356	37.46784225841675	32.29623425699895	15.769084547704937
6	34.66657515285666	33.60551542293679	16.821096221051608	14.90681320315494
7	30.71072376246683	31.586332807210177	19.26797396834111	18.434969461981883
8	28.793289540460755	34.032565885876444	18.576493377459773	18.59765119620303
9	28.5645674147948	15.140928871961526	17.973514505986003	38.320989207257675
10-11	27.214351894216694	24.75793260431918	26.29516065382504	21.732554847639094
12-13	26.879740702495212	22.19448528137089	26.661455267212897	24.264318748920992
14-15	24.31556119996125	23.470246710014845	24.191314374850098	28.022877715173806
16-17	24.602137255721907	26.418865379481016	24.74542395743645	24.233573407360627
18-19	24.490200689143034	25.64859995039771	26.557951411199397	23.303247949259863
20-21	25.18297195114936	24.853098972970212	26.199782538242506	23.764146537637927
22-23	24.73219459323515	24.514313558637827	26.256790250475664	24.496701597651356
24-25	24.305583341430932	24.69244608428742	26.304344408942033	24.697626165339614
26-27	24.093230190388613	24.938179251234807	26.354287012605642	24.614303545770934
28-29	24.296276555079306	25.558538172937666	26.08444181192934	24.060743460053686
30-31	24.97890503371265	24.9850852693904	26.159758735030792	23.87625096186616
32-33	24.451899288157694	24.915481007118423	26.214052856747315	24.418566847976564
34-35	24.481232188986706	25.255851622313813	26.27397085075956	23.988945337939917
36-37	24.47250103157974	25.206878627275504	26.210949571380855	24.10967076976391
38-39	24.59588325885513	25.29199754363039	26.148118801694597	23.964000395819884
40-41	24.620295875385242	24.94652972847069	26.24424884226636	24.18892555387771
42-43	24.445513792626713	25.03046391968028	26.021028053242105	24.5029942344509
44-45	24.157011752374537	25.163056739136724	26.239872476894117	24.440059031594622
46-47	24.180840167777767	25.11507838548374	25.9986588924824	24.70542255425609
48-49	24.195203785015195	25.37841399603965	25.87865531076371	24.547726908181446
50-51	24.298976946099444	25.399086760600813	25.866423555317713	24.43551273798203
52-53	24.518474565433053	25.391055774679284	25.665983515052616	24.424486144835047
54-55	24.15604838983375	24.960318778729366	26.16602773023414	24.717605101202743
56-57	24.273064112108667	25.301505670735803	25.763516670691505	24.661913546464024
58-59	24.24346779857435	25.406550098846637	25.920109627955128	24.429872474623888
60-61	24.566659831629455	25.349354946340195	25.981333918241045	24.1026513037893
62-63	24.50327316894553	25.40468514168847	25.883987818274374	24.20805387109162
64-65	24.311025661360354	25.010699080414934	25.953111235427638	24.72516402279708
66-67	24.229433550287634	25.263440681593035	25.83392367909912	24.67320208902021
68-69	24.07994724387846	25.24784803036192	25.964742563844624	24.707462161914997
70-71	23.97072703019963	25.129085566208197	26.186133277319453	24.714054126272725
72-73	24.079981766466446	25.550310477351335	25.73971916643029	24.629988589751925
74-75	24.06798165311323	25.458180951690267	25.708826947445434	24.765010447751063
76-77	23.971655325557933	25.011252990080223	25.894380506820387	25.12271117754146
78-79	23.85134717559848	25.15631580883942	26.114769365638065	24.877567649924035
80-81	24.02777688469928	25.411869942049925	25.668969380267825	24.891383792982975
82-83	24.175727207057612	25.383350707754616	25.775043281243416	24.665878803944363
84-85	24.111953753541556	25.487494386915415	25.51207228474066	24.888479574802368
86-87	23.80454298315368	25.649464545855295	25.62945958748347	24.916532883507557
88-89	23.750489858631354	25.324610604809745	25.77240480309737	25.152494733461534
90-91	24.076683867487592	25.200899515411972	25.84745747574827	24.874959141352164
92-93	23.913820068259774	25.497391139089476	25.81207627890627	24.776712513744478
94-95	23.799316880887133	25.229965929782555	25.8322410643826	25.138476124947708
96-97	23.665903134815053	25.172151902351754	25.868082844396685	25.293862118436504
98-99	23.574959061018376	25.281964556701563	25.67720791858501	25.465868463695053
100-101	23.508183814131073	25.361500314720864	25.882307972047357	25.248007899100706
102-103	23.35877088130708	25.227183905541224	26.046682104002684	25.367363109149014
104-105	23.576950858534225	25.242435089268554	25.830437972840407	25.350176079356814
106-107	23.587811047733904	25.210794598199758	25.850635643550916	25.35075871051542
108-109	23.518784926903347	25.12757931709297	25.906862543712773	25.446773212290914
110-111	23.424028464208078	25.5184411845083	25.78636482984169	25.27116552144193
112-113	23.293474544877128	25.530284546238185	25.823072133416257	25.353168775468433
114-115	23.28255646220885	25.541044816107995	25.779318159572405	25.39708056211075
116-117	23.14247602226871	25.39817609200132	25.85522480194794	25.60412308378202
118-119	23.18980783046298	25.227748748145483	25.93583202911354	25.646611392277997
120-121	23.080855478419235	25.300359491845793	26.00719698174033	25.611588047994644
122-123	22.948848431501222	25.481759702265126	25.836780959600524	25.732610906633123
124-125	22.791561070382464	25.341463248917094	25.845857973459648	26.021117707240794
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	23.0
2	23.5
3	48.0
4	49.5
5	53.0
6	128.0
7	299.5
8	499.0
9	577.5
10	503.0
11	428.5
12	427.5
13	474.0
14	552.0
15	591.5
16	607.5
17	654.5
18	700.5
19	760.0
20	812.5
21	885.5
22	1017.5
23	1203.0
24	1412.5
25	1771.5
26	2309.5
27	3075.5
28	4160.5
29	5342.0
30	6755.0
31	8484.0
32	10678.5
33	12611.0
34	14399.5
35	16671.5
36	19322.5
37	22136.0
38	24614.0
39	27513.5
40	30373.0
41	32670.0
42	35101.0
43	38839.5
44	43091.0
45	47254.5
46	50890.5
47	54149.5
48	58697.0
49	62929.0
50	65388.0
51	66124.5
52	65054.0
53	63803.0
54	62580.0
55	59389.5
56	55300.5
57	50943.5
58	46535.5
59	41790.5
60	35439.0
61	29805.0
62	24948.0
63	19206.0
64	14393.0
65	11205.5
66	8730.0
67	6800.5
68	5482.5
69	4429.0
70	3613.5
71	2833.5
72	2167.5
73	1922.0
74	1589.0
75	1220.5
76	899.5
77	584.5
78	365.5
79	206.0
80	124.5
81	72.5
82	48.0
83	27.0
84	13.5
85	9.5
86	7.0
87	3.5
88	1.5
89	4.0
90	3.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.022505578168303144
2	0.002286280956780002
3	0.02829272684015252
4	0.159825328134902
5	0.021505330249711893
6	0.06837408986370194
7	0.052941693405436925
8	0.04593995797529816
9	0.03372264411250503
10-11	0.06208681723255693
12-13	0.05690696193985224
14-15	0.05890745777703474
16-17	0.050691135588606606
18-19	0.03743785066727253
20-21	0.02332721038714596
22-23	0.0025720660763775024
24-25	0.004358223073861879
26-27	0.0030007437557737528
28-29	0.004429669353761254
30-31	0.002143388396981252
32-33	0.00825204532837782
34-35	0.004465392493710941
36-37	0.007180351129887194
38-39	0.0018933264173334392
40-41	0.0031079131756228152
42-43	0.0033222520153209406
44-45	0.004536838773610316
46-47	0.004536838773610316
48-49	0.002036218977132189
50-51	0.0014289255979875013
52-53	8.930784987421882E-4
54-55	0.004393946213811566
56-57	7.859090788931255E-4
58-59	7.144627989937506E-4
60-61	1.4289255979875012E-4
62-63	0.0011074173384403134
64-65	0.0
66-67	0.003536590855019065
68-69	3.572313994968753E-4
70-71	0.0
72-73	0.002429173516578752
74-75	0.004965516453006566
76-77	0.0017861569974843765
78-79	1.7861569974843766E-4
80-81	0.0
82-83	0.006144380071346255
84-85	0.0015360950178365637
86-87	0.0
88-89	0.0010359710585409383
90-91	7.501859389434382E-4
92-93	0.0
94-95	0.0031079131756228152
96-97	0.001214586758289376
98-99	6.430165190943756E-4
100-101	0.0
102-103	0.001143140478390001
104-105	0.0
106-107	6.07293379144688E-4
108-109	0.0023934503766290644
110-111	3.572313994968753E-5
112-113	2.500619796478127E-4
114-115	0.0
116-117	0.0015003718778868764
118-119	0.0021076652570315643
120-121	0.0027506817761259396
122-123	0.0013217561781384386
124-125	0.10306125875484853
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1399653.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.2428171952669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.11091357793423	55.05501055074722
2	9.776745779481057	12.952783668695217
3	2.8522684484332204	5.66826892264168
4	1.3164803400364629	3.488294660247928
5	0.7492471641726464	2.4816121465180365
6	0.4771063817614031	1.8962922497829504
7	0.34445241203579374	1.5972248714069084
8	0.22950646873828393	1.21625240430091
9	0.17789124249477298	1.0605615351498128
>10	0.9054170980688404	10.427548987844617
>50	0.04319292990902146	1.9428587586373902
>100	0.01612870372454782	1.753246825106065
>500	4.329688064788233E-4	0.21223994389133888
>1k	2.1648440323941164E-4	0.24780447502988756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1975	0.14110640280126574	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.10581194053097447	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.144627989937506E-5	0.0
3	0.0	0.0	0.0	7.144627989937506E-5	0.0
4	0.0	0.0	0.0	7.144627989937506E-5	0.0
5	0.0	0.0	0.0	7.144627989937506E-5	0.0
6	0.0	0.0	0.0	7.144627989937506E-5	0.0
7	0.0	0.0	0.0	7.144627989937506E-5	0.0
8	0.0	0.0	0.0	7.144627989937506E-5	0.0
9	0.0	0.0	0.0	7.144627989937506E-5	7.144627989937506E-5
10-11	0.0	0.0	0.0	7.144627989937506E-5	7.144627989937506E-5
12-13	0.0	0.0	0.0	7.144627989937506E-5	2.1433883969812517E-4
14-15	0.0	0.0	0.0	1.4289255979875012E-4	2.8578511959750024E-4
16-17	0.0	0.0	0.0	2.1433883969812517E-4	3.572313994968753E-4
18-19	0.0	0.0	0.0	2.8578511959750024E-4	3.572313994968753E-4
20-21	0.0	0.0	0.0	3.2150825954718775E-4	3.572313994968753E-4
22-23	0.0	0.0	0.0	3.929545394465628E-4	3.929545394465628E-4
24-25	0.0	0.0	0.0	5.715702391950005E-4	5.001239592956255E-4
26-27	0.0	0.0	0.0	8.573553587925007E-4	5.001239592956255E-4
28-29	0.0	0.0	0.0	0.0025363429364278147	5.001239592956255E-4
30-31	0.0	0.0	0.0	0.010824111404755322	5.001239592956255E-4
32-33	0.0	0.0	0.0	0.028114111140404085	5.001239592956255E-4
34-35	0.0	0.0	0.0	0.04904787115092098	5.001239592956255E-4
36-37	0.0	0.0	0.0	0.07673330461192882	5.001239592956255E-4
38-39	0.0	0.0	0.0	0.12620985344224606	5.001239592956255E-4
40-41	0.0	0.0	0.0	0.19201187722957047	5.001239592956255E-4
42-43	0.0	0.0	0.0	0.25477743412117143	5.001239592956255E-4
44-45	0.0	0.0	0.0	0.316792805073829	5.001239592956255E-4
46-47	0.0	0.0	0.0	0.3878461304337575	5.715702391950005E-4
48-49	0.0	0.0	0.0	0.4643650962059882	6.430165190943755E-4
50-51	0.0	0.0	0.0	0.5487074296272004	6.430165190943755E-4
52-53	0.0	0.0	0.0	0.639765713358954	7.501859389434382E-4
54-55	0.0	0.0	0.0	0.7390760424190853	7.859090788931256E-4
56-57	0.0	0.0	0.0	0.8396009582375059	7.859090788931256E-4
58-59	0.0	0.0	0.0	0.9513786631400782	7.859090788931256E-4
60-61	0.0	0.0	0.0	1.0701581034727894	7.859090788931256E-4
62-63	0.0	0.0	0.0	1.2195879978823323	7.859090788931256E-4
64-65	0.0	0.0	0.0	1.3489414876401509	7.859090788931256E-4
66-67	0.0	0.0	0.0	1.4926199565177942	8.573553587925007E-4
68-69	0.0	0.0	0.0	1.6549101813092246	9.288016386918758E-4
70-71	0.0	0.0	0.0	1.823809186991347	9.288016386918758E-4
72-73	0.0	0.0	0.0	1.97556108549762	0.001000247918591251
74-75	0.0	0.0	0.0	2.138351434248346	0.001000247918591251
76-77	0.0	0.0	0.0	2.3357217824703698	0.001000247918591251
78-79	0.0	0.0	0.0	2.5502035147282935	0.0010359710585409383
80-81	0.0	0.0	0.0	2.7591838834339653	0.0010716941984906258
82-83	0.0	0.0	0.0	2.975416049549424	0.0010716941984906258
84-85	0.0	0.0	0.0	3.201222017171399	0.0010716941984906258
86-87	0.0	0.0	0.0	3.4598575504071367	0.001143140478390001
88-89	0.0	0.0	0.0	3.752001388915681	0.001143140478390001
90-91	0.0	0.0	0.0	4.0551122313887795	0.001143140478390001
92-93	0.0	0.0	0.0	4.354436421027211	0.001143140478390001
94-95	0.0	0.0	0.0	4.655796829642776	0.001214586758289376
96-97	0.0	0.0	0.0	4.999203373979122	0.0013217561781384388
98-99	0.0	0.0	0.0	5.38458460775635	0.0014289255979875013
100-101	0.0	0.0	0.0	5.805546088923469	0.0014289255979875013
102-103	0.0	0.0	0.0	6.228615235347618	0.0014646487379371886
104-105	0.0	0.0	0.0	6.650791303273026	0.0015003718778868762
106-107	0.0	0.0	0.0	7.117728465555391	0.0015003718778868762
108-109	0.0	0.0	0.0	7.614387280275897	0.0015003718778868762
110-111	0.0	0.0	0.0	8.149341301022467	0.0015718181577862513
112-113	0.0	0.0	0.0	8.657860198206269	0.0015718181577862513
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3630	0.0	49.936794	1
TCAACGC	4420	0.0	40.184734	4
CAACGCA	4495	0.0	39.755245	5
GTACATG	7470	0.0	39.353195	1
TATCAAC	4645	0.0	38.628696	2
AACGCAG	4670	0.0	38.535755	6
ATCAACG	4640	0.0	38.412003	3
TACATGG	7570	0.0	38.113495	2
GGTATCA	2170	0.0	37.37084	1
ACATGGG	7655	0.0	37.14385	3
ATGGGGG	4000	0.0	35.293217	5
CATGGGG	6400	0.0	35.10981	4
ACGCAGA	5740	0.0	31.039707	7
CGCAGAG	5985	0.0	29.769073	8
TATCACG	125	4.821481E-5	28.61347	2
GCAGAGT	6550	0.0	27.110233	9
TGGGGGG	3020	0.0	26.637943	6
AGAGTAC	6120	0.0	23.367815	10-11
GAGTACT	3050	0.0	22.546707	12-13
GGATCGT	250	5.203656E-7	21.452423	6
>>END_MODULE