FastQCFastQC Report
Mon 15 Aug 2016
SRR2921586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795107
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39150.041.356151
GGTATCA25400.037.262021
GTACATG95250.035.746541
TCAACGC44950.034.158294
CAACGCA45600.033.9314545
TACATGG99650.033.5036132
ATCAACG47000.032.795033
ACATGGG101250.032.6802863
TATCAAC47800.032.3706632
AACGCAG48400.031.841976
CATGGGG88800.031.6326164
ATGGGGG52050.030.2983635
TATCACG1704.4190347E-728.0057472
ACGCAGA59500.025.8944857
CGCAGAG60150.025.711418
TATACCG1951.4633079E-624.4145855
GCAGAGT70400.021.5448579
GTAATAC3551.8280844E-920.1168023
TGGGGGG40500.019.9815626
TAACGCA2107.137497E-519.8374024
Produced by FastQC (version 0.11.5)