##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921586_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1795107
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.666820417947232	31.0	31.0	34.0	25.0	34.0
2	29.79089324480379	31.0	31.0	34.0	25.0	34.0
3	29.75929902785739	31.0	31.0	34.0	25.0	34.0
4	33.151062861433886	37.0	35.0	37.0	27.0	37.0
5	33.23725326679691	37.0	35.0	37.0	27.0	37.0
6	33.260370551727554	37.0	35.0	37.0	27.0	37.0
7	33.14190686126231	37.0	35.0	37.0	26.0	37.0
8	33.177338175384534	37.0	35.0	37.0	26.0	37.0
9	34.751738475756596	39.0	37.0	39.0	27.0	39.0
10-11	34.775078031560234	39.0	37.0	39.0	27.0	39.0
12-13	34.74446453609729	39.0	37.0	39.0	27.0	39.0
14-15	36.014400534341405	40.0	37.5	41.0	26.0	41.0
16-17	36.001305493210154	40.0	38.0	41.0	26.0	41.0
18-19	35.96030738000576	40.0	37.5	41.0	25.0	41.0
20-21	35.97436726612954	40.0	37.5	41.0	25.0	41.0
22-23	35.933145767912436	40.0	37.0	41.0	25.0	41.0
24-25	35.92076656154758	40.0	37.0	41.0	25.0	41.0
26-27	35.87056816111797	40.0	37.0	41.0	25.0	41.0
28-29	35.806560556000285	40.0	37.0	41.0	25.0	41.0
30-31	35.71583058837162	40.0	37.0	41.0	25.0	41.0
32-33	35.607371872540185	40.0	36.5	41.0	24.5	41.0
34-35	35.491227263890124	40.0	36.0	41.0	24.0	41.0
36-37	35.4041229854265	40.0	36.0	41.0	23.5	41.0
38-39	35.291108273768636	40.0	36.0	41.0	22.5	41.0
40-41	35.165160906842885	40.0	35.5	41.0	21.5	41.0
42-43	34.96212844136868	40.0	35.0	41.0	19.5	41.0
44-45	34.701812482487114	40.0	35.0	41.0	17.5	41.0
46-47	34.47372329337472	39.5	35.0	41.0	15.0	41.0
48-49	34.28900672773267	39.0	35.0	41.0	13.5	41.0
50-51	34.09531242427332	39.0	34.5	41.0	10.5	41.0
52-53	33.89722256110639	39.0	34.0	41.0	8.5	41.0
54-55	33.65570297480875	38.0	34.0	40.0	7.5	41.0
56-57	33.40890682282449	38.0	34.0	40.0	6.5	41.0
58-59	33.4037553193208	38.0	34.0	40.0	2.0	41.0
60-61	33.1879648399789	37.5	34.0	40.0	2.0	41.0
62-63	32.87688589036754	37.0	33.0	40.0	2.0	41.0
64-65	32.57486628930755	36.5	33.0	39.5	2.0	41.0
66-67	32.245735212441375	36.0	33.0	39.0	2.0	41.0
68-69	31.91016301535229	35.0	33.0	39.0	2.0	41.0
70-71	31.54311386452172	35.0	32.0	38.0	2.0	40.0
72-73	31.197332805231106	35.0	32.0	37.0	2.0	39.5
74-75	30.84205843997043	35.0	32.0	37.0	2.0	39.0
76-77	30.547464858640737	35.0	32.0	36.0	2.0	39.0
78-79	30.28061558447491	35.0	31.0	36.0	2.0	37.5
80-81	30.032792195674126	35.0	31.0	35.0	2.0	37.0
82-83	29.82404112958169	35.0	31.0	35.0	2.0	36.5
84-85	29.62072038045643	35.0	31.0	35.0	2.0	36.0
86-87	29.4565889387095	35.0	31.0	35.0	2.0	36.0
88-89	29.303359911136216	35.0	31.0	35.0	2.0	36.0
90-91	29.13490198634399	35.0	30.0	35.0	2.0	35.0
92-93	29.064656591501233	34.0	30.0	35.0	2.0	35.0
94-95	28.95259948292776	34.0	30.0	35.0	2.0	35.0
96-97	28.872957990805006	34.0	30.0	35.0	2.0	35.0
98-99	28.78276838093774	34.0	30.0	35.0	2.0	35.0
100-101	28.68114909027707	34.0	30.0	35.0	2.0	35.0
102-103	27.884820236342456	33.5	28.0	34.5	2.0	35.0
104-105	28.745913196260723	34.0	30.0	35.0	2.0	35.0
106-107	28.994673855096103	35.0	31.0	35.0	2.0	35.0
108-109	28.99284638742983	35.0	31.0	35.0	2.0	35.0
110-111	28.935035627402712	35.0	31.0	35.0	2.0	35.0
112-113	28.85749038915229	35.0	31.0	35.0	2.0	35.0
114-115	28.754082904250275	35.0	31.0	35.0	2.0	35.0
116-117	28.642410173878215	35.0	30.5	35.0	2.0	35.0
118-119	28.368999173865404	35.0	30.0	35.0	2.0	35.0
120-121	28.260848517664964	35.0	30.0	35.0	2.0	35.0
122-123	28.038417765626228	34.0	30.0	35.0	2.0	35.0
124-125	27.32371496518035	34.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	97309.0
3	14462.0
4	7081.0
5	5875.0
6	6177.0
7	6848.0
8	6937.0
9	7114.0
10	7523.0
11	7816.0
12	8201.0
13	8500.0
14	8454.0
15	7120.0
16	5734.0
17	5250.0
18	4845.0
19	4589.0
20	4678.0
21	4879.0
22	5580.0
23	7600.0
24	10986.0
25	13513.0
26	14868.0
27	16662.0
28	19983.0
29	23840.0
30	28703.0
31	35794.0
32	46503.0
33	63699.0
34	98621.0
35	164252.0
36	336045.0
37	534795.0
38	144183.0
39	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76785983931424	17.082181087133495	11.867053958832843	25.282905114719423
2	16.875505941487116	19.124741423160128	38.738418013940446	25.261334621412306
3	20.03811796848276	24.24301681268712	27.851298696234	27.86756652259612
4	14.487799252450372	15.163145547099024	34.022923443853834	36.326131756596766
5	14.85632686626871	37.731037564393	32.012749897205204	15.399885672133085
6	34.68848967393728	33.34738391542888	16.953867255452966	15.010259155180874
7	30.07622899466464	32.20134353881793	19.29001576305915	18.432411703458286
8	28.281333600856073	34.54493267344391	18.653357410379883	18.520376315320135
9	28.14925968604358	16.479870102371276	18.07377953510557	37.29709067647957
10-11	26.938864799188504	25.514903412067643	25.872635462763764	21.67359632598009
12-13	26.679218918968612	23.05600265641984	26.17944093727941	24.085337487332144
14-15	24.359799468749323	24.188455244052683	23.571811061091598	27.879934226106396
16-17	24.797626096223905	27.10209597314034	24.19654362953727	23.903734301098485
18-19	24.785327837607085	26.37998756105227	25.608014873236424	23.22666972810422
20-21	25.61729401174031	25.20811897336796	25.3266142367615	23.847972778130227
22-23	25.499326540972888	24.81617104131756	25.189067282931365	24.49543513477818
24-25	25.00494552676408	25.117424915758875	25.252054692232207	24.625574865244847
26-27	24.715358346557952	25.352843072644614	25.372432693767966	24.559365887029468
28-29	24.698376358201568	25.751443226317562	25.29299925515394	24.25718116032693
30-31	25.23862644777856	25.249464239744217	25.368262041650553	24.14364727082667
32-33	24.803743254719187	25.223143139260934	25.37574083856573	24.597372767454146
34-35	24.933380481811753	25.470209936123844	25.355403948490007	24.241005633574396
36-37	24.80277113484769	25.688375468158277	25.262259165734648	24.246594231259394
38-39	24.866526776550614	25.657531834659242	25.300154562605652	24.175786826184485
40-41	24.96686848376361	25.409560156133317	25.41206800849772	24.21150335160536
42-43	24.769344564410375	25.436383619194615	25.241813149386605	24.55245866700841
44-45	24.646690605995605	25.393285658967635	25.44242721394684	24.517596521089917
46-47	24.6252310963467	25.437815441637795	25.342618791044323	24.59433467097118
48-49	24.555988643740545	25.66021492194661	25.346998364542618	24.436798069770227
50-51	24.536603509690867	25.64606925489071	25.443322407051145	24.374004828367283
52-53	24.656836209411196	25.577706758464902	25.29085584710869	24.474601185015217
54-55	24.521392066792817	25.235863249463414	25.441554056312977	24.801190627430792
56-57	24.60837104028275	25.538189447923454	25.15844998318615	24.694989528607643
58-59	24.493055340814404	25.679902986215986	25.370425003614617	24.456616669354993
60-61	24.83590608813487	25.53159183909993	25.436785118883115	24.195716953882087
62-63	24.68300138548029	25.534404515111152	25.424022996799717	24.358571102608842
64-65	24.570769177621937	25.36852504176134	25.37370690279609	24.68699887782063
66-67	24.599191515172116	25.577687178391294	25.23861279851153	24.58450850792506
68-69	24.514824122003	25.544461222147312	25.37983852544422	24.56087613040547
70-71	24.445543602597667	25.445096353597012	25.566926424056145	24.54243361974918
72-73	24.529807353974846	25.71868593173351	25.251258346373927	24.500248367917717
74-75	24.4726775065803	25.66915170725715	25.276130322982848	24.5820404631797
76-77	24.474424572735348	25.408780887872506	25.309857236260935	24.806937303131207
78-79	24.447735695456014	25.51083577530414	25.376743299694592	24.664685229545256
80-81	24.46559769955797	25.711651893777837	25.211369269475377	24.61138113718882
82-83	24.56267726100063	25.71481107947422	25.27603960616715	24.446472053357997
84-85	24.614331204247144	25.76442217024165	25.08360486633946	24.537641759171745
86-87	24.461401484401858	25.785952394616217	25.21056698891295	24.542079132068974
88-89	24.334707643566386	25.655527593881715	25.21930319434496	24.790461568206936
90-91	24.57183993144424	25.5438172641383	25.279991263375518	24.60435154104194
92-93	24.542650739875977	25.64955377910297	25.42623441263135	24.381561068389708
94-95	24.489965332378777	25.50954717596081	25.372046671058733	24.62844082060168
96-97	24.418005063962546	25.5617284999578	25.346946063979537	24.673320372100125
98-99	24.41358586093526	25.564143925468464	25.181692352389152	24.840577861207127
100-101	24.237952057263417	25.745724213661457	25.334176818856193	24.682146910218933
102-103	24.038092733840823	25.63087016699623	25.52243250221931	24.808604596943635
104-105	24.143626848962953	25.692489776613325	25.428064711022376	24.73581866340135
106-107	24.20687367329482	25.65019521245527	25.410207980578193	24.73272313367172
108-109	24.196514307729636	25.633374125937436	25.43260542724565	24.737506139087273
110-111	24.078442994684938	25.92437023943686	25.38704252217302	24.61014424370518
112-113	24.005427095478886	26.028276381884353	25.369665867624995	24.596630655011765
114-115	23.972950762587924	26.094435545650814	25.37761682568424	24.554996866077026
116-117	23.96550220931486	25.86423444726182	25.402817304716653	24.767446038706673
118-119	24.011174055097666	25.80146974410341	25.406785716181123	24.780570484617797
120-121	23.929420867961326	25.99956603883423	25.50984169887719	24.561171394327253
122-123	23.87902556417784	26.08451386838543	25.416831468060096	24.619629099376635
124-125	23.880423523770748	25.98133301069539	25.440709550129636	24.69753391540422
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	389.0
1	342.0
2	264.5
3	189.0
4	150.5
5	165.0
6	160.0
7	211.5
8	304.0
9	330.5
10	333.5
11	335.0
12	360.5
13	392.5
14	421.5
15	487.5
16	559.5
17	630.0
18	660.5
19	705.5
20	826.5
21	968.0
22	1091.0
23	1231.5
24	1482.5
25	1873.5
26	2397.0
27	3152.5
28	4249.5
29	5742.5
30	7523.5
31	9547.0
32	12171.5
33	14987.0
34	17711.5
35	20645.0
36	23894.5
37	27572.0
38	31428.5
39	35291.5
40	39117.0
41	42640.0
42	46317.0
43	51754.5
44	57339.0
45	62402.5
46	66784.0
47	71226.5
48	76857.5
49	81038.5
50	82765.0
51	82672.5
52	81476.0
53	79707.0
54	78809.5
55	76490.0
56	71863.5
57	66448.5
58	60991.0
59	55156.5
60	47331.0
61	39824.5
62	32997.0
63	25564.5
64	19331.0
65	15004.5
66	11593.5
67	9113.5
68	7324.0
69	5698.5
70	4336.0
71	3371.5
72	2611.0
73	2025.0
74	1594.0
75	1187.0
76	883.0
77	622.0
78	408.5
79	278.0
80	187.5
81	147.0
82	114.5
83	89.0
84	84.5
85	69.5
86	60.5
87	64.5
88	60.0
89	44.5
90	38.0
91	35.5
92	27.5
93	24.5
94	24.0
95	21.0
96	21.5
97	22.5
98	16.0
99	6.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.023619761941767257
2	0.010361499342379033
3	0.008523168813892432
4	0.009804429485261881
5	0.0149851791564514
6	0.08840698632449208
7	0.05787955815447213
8	0.04829795661205711
9	0.025792334384524152
10-11	0.04941209632629141
12-13	0.0116984669994602
14-15	0.02640511122735302
16-17	0.03311780300561471
18-19	0.041390290383804415
20-21	0.028995486062947783
22-23	0.03565247085549775
24-25	0.030973084055713675
26-27	0.04398066521939918
28-29	0.04325647440514688
30-31	0.025680920413100723
32-33	0.028911925584380205
34-35	0.04320076741943516
36-37	0.009720869006694308
38-39	0.02033304978477606
40-41	0.041390290383804415
42-43	0.021419336006154508
44-45	0.016266439827820847
46-47	0.022338501270397812
48-49	0.027964906827281047
50-51	0.028104174291560338
52-53	0.024873169120280852
54-55	0.023564054956055545
56-57	0.02537453199168629
58-59	0.01729701906348758
60-61	0.02244991524182124
62-63	0.023536201463199687
64-65	0.021447189499010366
66-67	0.028716951134389202
68-69	0.022115673327550947
70-71	0.04481627000507491
72-73	0.0217535779204248
74-75	0.009275213121000587
76-77	0.016071465377829847
78-79	0.02506814357027186
80-81	0.017909795906316448
82-83	0.024343952756019558
84-85	0.011447785563757482
86-87	0.017269165570631722
88-89	0.02164216394900137
90-91	0.020249489306208488
92-93	0.008021805942486995
94-95	0.011392078578045766
96-97	0.014901618677883824
98-99	0.006044207949721103
100-101	0.009693015513838452
102-103	0.003704514549829063
104-105	0.004484412349793077
106-107	6.406303356847252E-4
108-109	0.014233134849343243
110-111	0.0011141397142343046
112-113	0.0022282794284686092
114-115	0.012924020685117934
116-117	0.0052086031640453745
118-119	0.28229515009411693
120-121	8.634582785315861E-4
122-123	9.47018757099159E-4
124-125	0.31705630917822725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1795107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.39122315046919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.63010956091775	54.686751563961586
2	9.001985651725708	11.773017050986352
3	2.8216466347860516	5.535327742411954
4	1.3728335746759799	3.5908506652037326
5	0.7426430203230376	2.428116773154109
6	0.5099811730298675	2.0008975612880455
7	0.35670158991625833	1.632760728503884
8	0.2799984798377353	1.4647554461489198
9	0.19544628394018354	1.1502424410356256
>10	1.0234311411266332	11.719876493016725
>50	0.04973194756122231	2.183104815189256
>100	0.014895339161756619	1.5865944727887673
>500	5.95602997819359E-4	0.24770424631118254
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	2.7853492855857616E-5
16-17	0.0	0.0	0.0	2.7853492855857616E-5	1.1141397142343046E-4
18-19	0.0	0.0	0.0	5.570698571171523E-5	1.1141397142343046E-4
20-21	0.0	0.0	0.0	8.356047856757285E-5	1.3926746427928808E-4
22-23	0.0	0.0	0.0	1.671209571351457E-4	2.2282794284686093E-4
24-25	0.0	0.0	0.0	4.735093785495795E-4	2.7853492855857615E-4
26-27	0.0	0.0	0.0	6.684838285405828E-4	3.8994889998200666E-4
28-29	0.0	0.0	0.0	0.0020054514856217487	3.8994889998200666E-4
30-31	0.0	0.0	0.0	0.00818892689962214	3.8994889998200666E-4
32-33	0.0	0.0	0.0	0.022589182706100528	4.4565588569372186E-4
34-35	0.0	0.0	0.0	0.03969122731959711	4.4565588569372186E-4
36-37	0.0	0.0	0.0	0.06205758208285077	4.4565588569372186E-4
38-39	0.0	0.0	0.0	0.10124744653104244	4.4565588569372186E-4
40-41	0.0	0.0	0.0	0.14840340993600937	4.4565588569372186E-4
42-43	0.0	0.0	0.0	0.1943338196553186	4.735093785495795E-4
44-45	0.0	2.7853492855857616E-5	0.0	0.249817977424187	5.013628714054372E-4
46-47	0.0	5.570698571171523E-5	0.0	0.30881167529289344	5.013628714054372E-4
48-49	0.0	5.570698571171523E-5	0.0	0.36685835440450065	7.241908142522981E-4
50-51	0.0	5.570698571171523E-5	0.0	0.4298351017515948	7.241908142522981E-4
52-53	0.0	5.570698571171523E-5	0.0	0.4975469428841846	7.520443071081556E-4
54-55	0.0	5.570698571171523E-5	0.0	0.5740604877592255	8.07751292819871E-4
56-57	0.0	5.570698571171523E-5	0.0	0.6594035898695731	8.356047856757286E-4
58-59	0.0	5.570698571171523E-5	0.0	0.7508466069153538	8.356047856757286E-4
60-61	2.7853492855857616E-5	5.570698571171523E-5	0.0	0.8449914127681526	8.356047856757286E-4
62-63	5.570698571171523E-5	5.570698571171523E-5	0.0	0.9441219938421498	8.913117713874437E-4
64-65	5.570698571171523E-5	5.570698571171523E-5	0.0	1.047931961715931	9.47018757099159E-4
66-67	5.570698571171523E-5	5.570698571171523E-5	0.0	1.1667549622390196	9.748722499550166E-4
68-69	5.570698571171523E-5	5.570698571171523E-5	0.0	1.3044904844112355	0.0010584327285225895
70-71	5.570698571171523E-5	5.570698571171523E-5	0.0	1.4404990900263885	0.0010584327285225895
72-73	5.570698571171523E-5	5.570698571171523E-5	0.0	1.5732766904702618	0.0012812606713694505
74-75	5.570698571171523E-5	5.570698571171523E-5	0.0	1.7143824852780365	0.0012812606713694505
76-77	5.570698571171523E-5	5.570698571171523E-5	0.0	1.889497394862813	0.0012812606713694505
78-79	5.570698571171523E-5	5.570698571171523E-5	0.0	2.07906826723978	0.0012812606713694505
80-81	5.570698571171523E-5	5.570698571171523E-5	0.0	2.263124148031287	0.001309114164225308
82-83	5.570698571171523E-5	5.570698571171523E-5	0.0	2.454700471893876	0.0013926746427928808
84-85	5.570698571171523E-5	5.570698571171523E-5	0.0	2.6500648707848615	0.0014205281356487384
86-87	5.570698571171523E-5	5.570698571171523E-5	0.0	2.8813045684741914	0.0015040886142163113
88-89	5.570698571171523E-5	5.570698571171523E-5	0.0	3.152151933004551	0.0015040886142163113
90-91	5.570698571171523E-5	5.570698571171523E-5	0.0	3.4219687182992433	0.0015040886142163113
92-93	5.570698571171523E-5	5.570698571171523E-5	0.0	3.680309864537323	0.0015040886142163113
94-95	5.570698571171523E-5	5.570698571171523E-5	0.0	3.945001607146538	0.0015040886142163113
96-97	5.570698571171523E-5	5.570698571171523E-5	0.0	4.253980403396566	0.0015597955999280266
98-99	5.570698571171523E-5	5.570698571171523E-5	0.0	4.613596849658544	0.0016155025856397418
100-101	5.570698571171523E-5	5.570698571171523E-5	0.0	4.991429480248252	0.0016155025856397418
102-103	5.570698571171523E-5	5.570698571171523E-5	0.0	5.360989623459771	0.001671209571351457
104-105	5.570698571171523E-5	5.570698571171523E-5	0.0	5.745562799320597	0.0017269165570631723
106-107	5.570698571171523E-5	5.570698571171523E-5	0.0	6.173336742600859	0.00175477004991903
108-109	5.570698571171523E-5	5.570698571171523E-5	0.0	6.645453446507646	0.0017826235427748874
110-111	5.570698571171523E-5	5.570698571171523E-5	0.0	7.142276198577578	0.0018661840213424602
112-113	5.570698571171523E-5	5.570698571171523E-5	0.0	7.626286343933815	0.001894037514198318
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3915	0.0	41.35615	1
GGTATCA	2540	0.0	37.26202	1
GTACATG	9525	0.0	35.74654	1
TCAACGC	4495	0.0	34.15829	4
CAACGCA	4560	0.0	33.931454	5
TACATGG	9965	0.0	33.503613	2
ATCAACG	4700	0.0	32.79503	3
ACATGGG	10125	0.0	32.680286	3
TATCAAC	4780	0.0	32.370663	2
AACGCAG	4840	0.0	31.84197	6
CATGGGG	8880	0.0	31.632616	4
ATGGGGG	5205	0.0	30.298363	5
TATCACG	170	4.4190347E-7	28.005747	2
ACGCAGA	5950	0.0	25.894485	7
CGCAGAG	6015	0.0	25.71141	8
TATACCG	195	1.4633079E-6	24.414585	5
GCAGAGT	7040	0.0	21.544857	9
GTAATAC	355	1.8280844E-9	20.116802	3
TGGGGGG	4050	0.0	19.981562	6
TAACGCA	210	7.137497E-5	19.837402	4
>>END_MODULE