FastQCFastQC Report
Mon 15 Aug 2016
SRR2921586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1795107
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.14066013892208096No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19520.10874003610926815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30900.045.369971
GTATCAA46900.043.6295131
TATAACG1508.367351E-1139.7655682
GTACATG101400.037.06481
TACATGG102400.036.115212
ATGGGGG51050.035.3579945
ACATGGG107050.034.1545033
CATGGGG91350.034.0809634
TCAACGC61100.032.7980464
CAACGCA63250.031.7402365
TATCAAC63250.031.6867122
ATCAACG64500.030.978423
AACGCAG66150.030.533976
GTATCGC1454.025296E-628.7636176
TGGGGGG36550.026.8973836
ACGCAGA77850.025.8677947
ATAACGC1859.069172E-725.7924393
TATCACG1401.0385205E-425.5635782
CGCAGAG83600.024.1571858
AGAGTAC83150.022.24601610-11
Produced by FastQC (version 0.11.5)