##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1795107
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65804935304692	33.0	31.0	34.0	30.0	34.0
2	31.826938449908557	33.0	31.0	34.0	30.0	34.0
3	31.88994862144708	34.0	31.0	34.0	30.0	34.0
4	35.283140782137224	37.0	35.0	37.0	33.0	37.0
5	35.118462019255674	37.0	35.0	37.0	33.0	37.0
6	35.13598576575101	37.0	35.0	37.0	33.0	37.0
7	35.021856078774135	37.0	35.0	37.0	33.0	37.0
8	35.05316284767426	37.0	35.0	37.0	33.0	37.0
9	36.76241806198739	39.0	38.0	39.0	33.0	39.0
10-11	36.70304110005699	39.0	38.0	39.0	33.0	39.0
12-13	36.631623073165	39.0	38.0	39.0	33.0	39.0
14-15	38.0516905120419	41.0	39.0	41.0	33.0	41.0
16-17	38.06265754631897	41.0	39.0	41.0	33.0	41.0
18-19	38.076288488652764	41.0	39.0	41.0	33.5	41.0
20-21	38.013419255788094	41.0	39.0	41.0	33.0	41.0
22-23	38.02020631639228	41.0	39.0	41.0	33.0	41.0
24-25	37.983363387252126	41.0	39.0	41.0	33.0	41.0
26-27	37.90636212771717	41.0	39.0	41.0	33.0	41.0
28-29	37.837608287416856	41.0	38.5	41.0	33.0	41.0
30-31	37.7248403577057	41.0	38.0	41.0	33.0	41.0
32-33	37.60430910246576	41.0	38.0	41.0	32.5	41.0
34-35	37.472447881936844	41.0	38.0	41.0	32.0	41.0
36-37	37.396237383064076	40.0	38.0	41.0	32.0	41.0
38-39	37.314128071474286	40.0	38.0	41.0	32.0	41.0
40-41	37.19358177534821	40.0	38.0	41.0	31.0	41.0
42-43	37.0348048890679	40.0	37.5	41.0	31.0	41.0
44-45	36.87466875233621	40.0	37.0	41.0	31.0	41.0
46-47	36.67357628263942	40.0	37.0	41.0	30.0	41.0
48-49	36.476705009784936	40.0	36.5	41.0	30.0	41.0
50-51	36.23426653675797	40.0	36.0	41.0	29.0	41.0
52-53	36.106475547140086	40.0	36.0	41.0	29.0	41.0
54-55	35.9776030063946	40.0	35.0	41.0	29.0	41.0
56-57	35.77787786466211	39.0	35.0	41.0	28.0	41.0
58-59	35.583590838874784	39.0	35.0	41.0	28.0	41.0
60-61	35.32923803427874	39.0	35.0	41.0	28.0	41.0
62-63	34.97692505237849	37.5	35.0	40.5	28.0	41.0
64-65	34.68844586980052	37.0	35.0	40.0	28.0	41.0
66-67	34.380939687717785	36.5	35.0	39.5	28.0	41.0
68-69	34.0142005462627	36.0	35.0	39.0	26.5	41.0
70-71	33.64671743801344	35.5	34.5	39.0	26.0	41.0
72-73	33.32882440990983	35.0	34.0	37.5	26.0	40.0
74-75	33.010655353692	35.0	34.0	37.0	26.0	39.0
76-77	32.68459846683234	35.0	34.0	36.5	26.0	39.0
78-79	32.42774776099698	35.0	34.0	36.0	26.0	38.0
80-81	32.18065246249945	35.0	34.0	36.0	25.0	37.0
82-83	31.96686604196853	35.0	34.0	35.0	25.0	37.0
84-85	31.756573006511587	35.0	34.0	35.0	24.5	36.0
86-87	31.62924020685118	35.0	34.0	35.0	24.0	36.0
88-89	31.534238906093062	35.0	33.5	35.0	24.0	36.0
90-91	31.434485520918805	35.0	33.0	35.0	24.0	36.0
92-93	31.356657848250826	35.0	33.0	35.0	24.0	35.0
94-95	31.291936358111244	35.0	33.0	35.0	23.5	35.0
96-97	31.231198474519903	35.0	33.0	35.0	23.0	35.0
98-99	31.153630953475197	35.0	33.0	35.0	22.5	35.0
100-101	31.087559683071817	35.0	33.0	35.0	20.0	35.0
102-103	30.40723310643878	34.0	31.5	35.0	19.5	35.0
104-105	30.919808401393343	35.0	33.0	35.0	20.0	35.0
106-107	31.022286415238757	35.0	33.0	35.0	20.0	35.0
108-109	31.00857748312496	35.0	33.0	35.0	20.0	35.0
110-111	30.944281594356212	35.0	33.0	35.0	19.0	35.0
112-113	30.916048179857803	35.0	33.0	35.0	19.0	35.0
114-115	30.796425227019895	35.0	33.0	35.0	18.0	35.0
116-117	30.98027833438341	35.0	33.0	35.0	19.0	35.0
118-119	30.971239597416755	35.0	33.0	35.0	18.5	35.0
120-121	30.89575078254388	35.0	33.0	35.0	16.0	35.0
122-123	30.770614509330088	35.0	33.0	35.0	2.0	35.0
124-125	30.14068604267044	34.5	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32924.0
3	9128.0
4	4753.0
5	3593.0
6	3733.0
7	4242.0
8	4626.0
9	4992.0
10	5145.0
11	5631.0
12	6055.0
13	7558.0
14	10127.0
15	9267.0
16	5299.0
17	4618.0
18	4270.0
19	3704.0
20	3646.0
21	3600.0
22	4210.0
23	5211.0
24	7024.0
25	9160.0
26	10752.0
27	12388.0
28	14925.0
29	18177.0
30	21678.0
31	27353.0
32	35589.0
33	48894.0
34	76246.0
35	136257.0
36	319079.0
37	669374.0
38	241484.0
39	395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.020615971806265	17.517502681469306	11.052668236080738	27.409213110643694
2	15.98597712508781	19.519215717296344	37.40562329985455	27.089183857761295
3	18.881230716422685	23.5946009048271	28.181870358637624	29.342298020112594
4	13.461287359759657	15.167551846937283	33.85276061295618	37.51840018034688
5	14.797001463162951	37.04125718348376	32.296452637760744	15.865288715592548
6	34.45859656027216	33.44690206536408	16.86635030193637	15.228151072427382
7	30.432434450958677	31.451055099484947	19.197788864173322	18.91872158538305
8	28.216573075662698	34.29805632402476	18.453162513960628	19.03220808635191
9	28.158579418712275	15.620387031054875	18.289772325167604	37.931261225065235
10-11	26.988648556753038	24.910328336245747	25.99600344472965	22.105019662271573
12-13	26.921595556947786	22.348114523193477	26.532650124130335	24.197639795728403
14-15	24.42652633914102	23.490257030241263	24.061243355609268	28.02197327500845
16-17	24.51812305418357	26.61757050641109	24.664034902916786	24.20027153648855
18-19	24.534532710462656	25.64624318816464	26.513900630096508	23.305323471276193
20-21	25.332248289342342	24.643054051516557	26.280036629985602	23.744661029155495
22-23	25.05462935185982	24.281921716452146	26.19217331647588	24.471275615212154
24-25	24.48274411700558	24.677198179078793	26.224724941233426	24.6153327626822
26-27	24.297581848740617	24.917648487590032	26.335464937168844	24.4493047265005
28-29	24.299265610136892	25.291785223346157	26.294611085345487	24.114338081171457
30-31	24.873563179971327	24.82220018032758	26.233345672709063	24.07089096699203
32-33	24.5584346523398	24.74022249651035	26.24784151308992	24.45350133805993
34-35	24.510057071453662	25.024198753168818	26.22359341526509	24.242150760112434
36-37	24.579140523262698	25.117946503805737	26.18358066560352	24.119332307328044
38-39	24.6111147940439	25.136972603251024	26.2040079841031	24.04790461860198
40-41	24.641639309010507	24.879738658944685	26.268670103155962	24.20995192888885
42-43	24.545316792206975	24.839516200465617	26.037399702848013	24.577767304479398
44-45	24.406038913107057	24.942744530020473	26.13991448587067	24.511302071001797
46-47	24.446651160847342	24.993050236069138	25.8974387543349	24.662859848748624
48-49	24.361103056627705	25.300037630917853	25.827427563268547	24.511431749185896
50-51	24.439941395369566	25.337171602379836	25.790521870891546	24.43236513135905
52-53	24.592847939185255	25.313201392018563	25.588452061761775	24.505498607034408
54-55	24.386328589828725	24.934819522500078	25.845229234725487	24.83362265294571
56-57	24.42212620122573	25.202670501564405	25.579921045895382	24.795282251314486
58-59	24.307000079104494	25.3255539821313	25.812965783407442	24.554480155356767
60-61	24.66099457190224	25.176257197354584	25.901006292671624	24.261741938071555
62-63	24.537676512688357	25.195442792438598	25.88755274455522	24.379327950317826
64-65	24.401275244316913	24.933611199778063	25.824282340829825	24.8408312150752
66-67	24.34350862239455	25.222265861111342	25.746378204324706	24.6878473121694
68-69	24.277594144712637	25.206374814041926	25.846507887741904	24.669523153503533
70-71	24.13805973682906	25.027756005630863	26.067694014897164	24.76649024264292
72-73	24.308730537634528	25.335711703240044	25.66032354224104	24.695234216884383
74-75	24.152062111963872	25.298625388680275	25.70502833817962	24.844284161176233
76-77	24.139771318747126	24.955698229878976	25.80017548013314	25.104354971240756
78-79	24.051963534166525	25.112514309747898	25.84160815105523	24.993914005030344
80-81	24.085360928345775	25.36882202565084	25.640588555445444	24.905228490557946
82-83	24.222564406541714	25.274316937455293	25.690165592945753	24.812953063057243
84-85	24.160593578663434	25.292104298531875	25.54944687640314	24.99785524640155
86-87	24.000101386713997	25.41299766532023	25.64039358099545	24.946507366970323
88-89	23.897789883662988	25.221570102610652	25.65990552544404	25.22073448828232
90-91	24.017530010979932	25.126219087256203	25.75646498537959	25.099785916384278
92-93	23.952694741873326	25.300191019254004	25.81595414646592	24.93116009240675
94-95	23.888138059510254	25.098743657565354	25.80471206405163	25.20840621887277
96-97	23.75690115290094	25.13305113614682	25.81346630415739	25.29658140679485
98-99	23.781949955225148	25.181181471149817	25.563585163438653	25.473283410186383
100-101	23.58828749484014	25.24771503871357	25.796066752566837	25.36793071387945
102-103	23.421169764673312	25.10819858067528	25.96988767625269	25.50074397839872
104-105	23.646863390316007	25.119644678562338	25.823001080158452	25.410490850963203
106-107	23.600410673957633	25.090552159269297	25.796279646013755	25.512757520759312
108-109	23.550426920949295	25.02711603993843	25.82775646314363	25.594700575968645
110-111	23.382692549267993	25.334360571008226	25.76021297912574	25.522733900598045
112-113	23.2949028551565	25.325928372912305	25.844728686877577	25.534440085053618
114-115	23.238754012991983	25.35709570515852	25.819797928480032	25.584352353369468
116-117	23.099163378972946	25.144924064581037	25.958370611193498	25.797541945252515
118-119	23.187696928911727	25.069329004425466	25.89520485063485	25.847769216027956
120-121	23.01693567862627	25.16333051355208	26.024166927336275	25.795566880485378
122-123	22.88724087265941	25.234452359564646	25.849631285383023	26.02867548239292
124-125	22.69167101395976	25.143404907761642	25.930992217253312	26.23393186102529
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	68.0
1	51.5
2	44.0
3	58.5
4	55.0
5	55.0
6	136.0
7	340.5
8	565.0
9	661.0
10	597.5
11	525.5
12	552.5
13	597.5
14	636.0
15	702.0
16	767.0
17	816.5
18	905.0
19	985.5
20	1033.0
21	1115.0
22	1261.5
23	1475.0
24	1685.5
25	2025.5
26	2549.5
27	3260.0
28	4306.0
29	5599.0
30	7304.5
31	9350.0
32	11863.0
33	14390.0
34	17122.0
35	20201.0
36	23605.5
37	27171.0
38	30577.5
39	34406.5
40	38373.0
41	42163.5
42	46123.0
43	51299.5
44	56840.5
45	62243.5
46	66518.0
47	71374.5
48	77434.0
49	81695.5
50	83828.5
51	83763.0
52	82102.0
53	80553.5
54	80152.5
55	77923.0
56	73377.0
57	67649.5
58	61922.5
59	55994.0
60	47858.0
61	40010.0
62	32931.0
63	25145.5
64	18469.5
65	14162.0
66	10984.5
67	8514.5
68	6721.5
69	5143.5
70	3817.5
71	2849.5
72	2206.5
73	1757.0
74	1326.0
75	976.5
76	646.5
77	374.0
78	222.5
79	116.5
80	62.0
81	32.0
82	20.0
83	14.5
84	8.5
85	4.0
86	1.5
87	2.5
88	2.5
89	2.5
90	2.5
91	2.5
92	2.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021280068541875218
2	0.0022282794284686092
3	0.02841056271297477
4	0.16739949206370427
5	0.02033304978477606
6	0.06606848505409427
7	0.051027598911931156
8	0.04317291392657931
9	0.031975809798524546
10-11	0.05916081882584158
12-13	0.057099660354508114
14-15	0.05673756494738196
16-17	0.0486321985263274
18-19	0.034538331141263444
20-21	0.022422061748965383
22-23	0.0030917377070001956
24-25	0.004540119335504792
26-27	0.0032310051712794836
28-29	0.004428705364081361
30-31	0.002256132921324467
32-33	0.00877385024959515
34-35	0.004261584406946216
36-37	0.0066569847925499704
38-39	0.0022282794284686092
40-41	0.0032310051712794836
42-43	0.0036766610569732056
44-45	0.004567972828360649
46-47	0.004567972828360649
48-49	0.002256132921324467
50-51	0.0015040886142163113
52-53	8.913117713874437E-4
54-55	0.0050693356997660866
56-57	7.520443071081556E-4
58-59	7.241908142522981E-4
60-61	1.671209571351457E-4
62-63	9.191652642433014E-4
64-65	0.0
66-67	0.004010902971243497
68-69	6.406303356847252E-4
70-71	0.0
72-73	0.0022282794284686092
74-75	0.0050414822069102284
76-77	0.0017826235427748874
78-79	1.1141397142343046E-4
80-81	0.0
82-83	0.005737819528306669
84-85	0.0016155025856397418
86-87	0.0
88-89	0.0011419932070901623
90-91	7.798977999640132E-4
92-93	0.0
94-95	0.003063884214144338
96-97	0.0014762351213604536
98-99	8.077512928198709E-4
100-101	0.0
102-103	0.0015597955999280264
104-105	0.0
106-107	5.013628714054372E-4
108-109	0.0024511073713154702
110-111	8.356047856757285E-5
112-113	2.2282794284686093E-4
114-115	0.0
116-117	0.0016155025856397418
118-119	0.002395400385603755
120-121	0.002813202778441619
122-123	0.001086286221378447
124-125	0.10280724213097046
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1795107.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.68408659855815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20389081732876	50.08987833315969
2	10.29015617491032	12.694777692117123
3	3.2196928611371503	5.958114398014303
4	1.5885910969903894	3.919631631858146
5	0.8933501237386325	2.7552743197763228
6	0.5946276941402545	2.200743970754905
7	0.39474497730901253	1.70446383552725
8	0.30090816026362066	1.4848996012731193
9	0.23181430764502953	1.2869328444804722
>10	1.2030018986134834	12.995831838915572
>50	0.060190393821464856	2.5304659005728776
>100	0.018216058506267736	1.8365904379339868
>500	5.436237304870035E-4	0.22159680068195423
>1k	2.7181186524350163E-4	0.32079839493427914
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2525	0.14066013892208096	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1952	0.10874003610926815	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.570698571171523E-5	0.0
3	0.0	0.0	0.0	5.570698571171523E-5	0.0
4	0.0	0.0	0.0	5.570698571171523E-5	0.0
5	0.0	0.0	0.0	5.570698571171523E-5	0.0
6	0.0	0.0	0.0	5.570698571171523E-5	0.0
7	0.0	0.0	0.0	1.1141397142343046E-4	0.0
8	0.0	0.0	0.0	1.1141397142343046E-4	0.0
9	0.0	0.0	0.0	1.1141397142343046E-4	0.0
10-11	0.0	0.0	0.0	1.1141397142343046E-4	0.0
12-13	0.0	0.0	0.0	1.1141397142343046E-4	1.1141397142343046E-4
14-15	0.0	0.0	0.0	1.1141397142343046E-4	3.063884214144338E-4
16-17	0.0	0.0	0.0	1.3926746427928808E-4	4.4565588569372186E-4
18-19	0.0	0.0	0.0	1.671209571351457E-4	4.4565588569372186E-4
20-21	0.0	0.0	0.0	1.9497444999100333E-4	5.013628714054371E-4
22-23	0.0	0.0	0.0	2.7853492855857615E-4	6.684838285405828E-4
24-25	0.0	0.0	0.0	5.8492334997301E-4	7.798977999640133E-4
26-27	0.0	0.0	0.0	8.077512928198709E-4	7.798977999640133E-4
28-29	0.0	0.0	0.0	0.0021447189499010366	7.798977999640133E-4
30-31	0.0	0.0	0.0	0.008300340871045569	7.798977999640133E-4
32-33	0.0	0.0	0.0	0.022839864141803244	7.798977999640133E-4
34-35	0.0	0.0	0.0	0.04019259019100254	7.798977999640133E-4
36-37	0.0	0.0	0.0	0.06294889385423821	7.798977999640133E-4
38-39	0.0	0.0	0.0	0.10174880940244788	7.798977999640133E-4
40-41	0.0	0.0	0.0	0.14965681711452297	7.798977999640133E-4
42-43	0.0	0.0	0.0	0.19628356415522863	7.798977999640133E-4
44-45	0.0	0.0	0.0	0.2515170404883943	8.07751292819871E-4
46-47	0.0	0.0	0.0	0.31062215232852414	8.913117713874438E-4
48-49	0.0	0.0	0.0	0.36900307335440174	0.0010584327285225895
50-51	0.0	0.0	0.0	0.43111636242296425	0.0010584327285225895
52-53	0.0	0.0	0.0	0.4994131269055271	0.0012534071785135928
54-55	0.0	0.0	0.0	0.5766230091019644	0.0013648211499370233
56-57	0.0	0.0	0.0	0.6628574229836997	0.0013926746427928808
58-59	0.0	5.570698571171523E-5	0.0	0.7559716496008315	0.0014483816285045961
60-61	0.0	5.570698571171523E-5	0.0	0.8517041045464142	0.0014483816285045961
62-63	0.0	5.570698571171523E-5	0.0	0.9524223347131954	0.0014483816285045961
64-65	0.0	1.1141397142343046E-4	0.0	1.0573185888083552	0.0014483816285045961
66-67	0.0	1.1141397142343046E-4	0.0	1.1776735314385158	0.0014483816285045961
68-69	0.0	1.1141397142343046E-4	0.0	1.3158547094964255	0.0014483816285045961
70-71	0.0	1.3926746427928808E-4	0.0	1.4532559897543713	0.0014483816285045961
72-73	0.0	1.671209571351457E-4	0.0	1.5878440672338752	0.0015876490927838843
74-75	0.0	1.671209571351457E-4	0.0	1.7311224344844067	0.0016155025856397418
76-77	0.0	1.671209571351457E-4	0.0	1.9091898143119046	0.0016155025856397418
78-79	0.0	1.671209571351457E-4	0.0	2.101267501045899	0.0016155025856397418
80-81	0.0	1.671209571351457E-4	0.0	2.2863818145659285	0.0016155025856397418
82-83	0.0	1.671209571351457E-4	0.0	2.481356264556932	0.0016712095713514571
84-85	0.0	1.671209571351457E-4	0.0	2.6828762853690615	0.0016712095713514571
86-87	0.0	1.671209571351457E-4	0.0	2.919798095600987	0.00175477004991903
88-89	0.0	1.671209571351457E-4	0.0	3.196327572673941	0.0018383305284866028
90-91	0.0	1.671209571351457E-4	0.0	3.472077151946931	0.0018383305284866028
92-93	0.0	1.671209571351457E-4	0.0	3.735821875799047	0.0019497444999100333
94-95	0.0	2.2282794284686093E-4	0.0	4.0056943680794515	0.0020611584713334636
96-97	0.0	2.2282794284686093E-4	0.0	4.320243862900652	0.002116865457045179
98-99	0.0	2.2282794284686093E-4	0.0	4.68348126323389	0.0021725724427568943
100-101	0.0	2.2282794284686093E-4	0.0	5.066717471437636	0.0021725724427568943
102-103	2.7853492855857616E-5	2.2282794284686093E-4	0.0	5.438004531206218	0.0021725724427568943
104-105	5.570698571171523E-5	2.2282794284686093E-4	0.0	5.826115100659738	0.0022282794284686092
106-107	5.570698571171523E-5	2.2282794284686093E-4	0.0	6.259097647104044	0.0022839864141803246
108-109	5.570698571171523E-5	2.2282794284686093E-4	0.0	6.737732068339102	0.0022839864141803246
110-111	5.570698571171523E-5	2.2282794284686093E-4	0.0	7.243133696208638	0.00233969339989204
112-113	5.570698571171523E-5	2.2282794284686093E-4	0.0	7.736948271050138	0.00233969339989204
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3090	0.0	45.36997	1
GTATCAA	4690	0.0	43.629513	1
TATAACG	150	8.367351E-11	39.765568	2
GTACATG	10140	0.0	37.0648	1
TACATGG	10240	0.0	36.11521	2
ATGGGGG	5105	0.0	35.357994	5
ACATGGG	10705	0.0	34.154503	3
CATGGGG	9135	0.0	34.080963	4
TCAACGC	6110	0.0	32.798046	4
CAACGCA	6325	0.0	31.740236	5
TATCAAC	6325	0.0	31.686712	2
ATCAACG	6450	0.0	30.97842	3
AACGCAG	6615	0.0	30.53397	6
GTATCGC	145	4.025296E-6	28.763617	6
TGGGGGG	3655	0.0	26.897383	6
ACGCAGA	7785	0.0	25.867794	7
ATAACGC	185	9.069172E-7	25.792439	3
TATCACG	140	1.0385205E-4	25.563578	2
CGCAGAG	8360	0.0	24.157185	8
AGAGTAC	8315	0.0	22.246016	10-11
>>END_MODULE