FastQCFastQC Report
Mon 15 Aug 2016
SRR2921585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867873
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG95900.039.848661
GTATCAA31500.039.3052061
TACATGG98150.038.1981932
ACATGGG99500.037.3210683
CATGGGG84450.036.150164
TCAACGC35000.033.8358884
GGTATCA20550.033.6003231
ATGGGGG51400.033.343435
CAACGCA35900.032.820995
AACGCAG38800.030.6721536
ATCAACG38700.030.6009333
TATCAAC40750.029.7916892
ACGCAGA46700.025.4759967
GTACCGT3101.2732926E-1124.9532816
GAGTACA64250.024.4584031
AGTACAT64350.024.0445882
TGGGGGG41550.023.629746
TATCACG1802.1750637E-523.1429162
CGCAGAG51500.023.0996768
ATGGGGA34050.021.3217895
Produced by FastQC (version 0.11.5)