##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921585_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1867873
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.322254243195335	31.0	31.0	34.0	27.0	34.0
2	30.29070552441199	31.0	31.0	34.0	27.0	34.0
3	30.499486849480665	31.0	31.0	34.0	27.0	34.0
4	33.99203746721538	37.0	35.0	37.0	30.0	37.0
5	34.03439741352865	37.0	35.0	37.0	30.0	37.0
6	33.936807802243514	37.0	35.0	37.0	30.0	37.0
7	33.949519051884145	37.0	35.0	37.0	30.0	37.0
8	33.96647416607018	37.0	35.0	37.0	30.0	37.0
9	35.6380985216875	39.0	37.0	39.0	30.0	39.0
10-11	35.66693426159058	39.0	37.0	39.0	30.0	39.0
12-13	35.604647639320234	39.0	37.0	39.0	30.0	39.0
14-15	36.92038859172974	40.5	38.0	41.0	30.0	41.0
16-17	36.87703178963452	40.5	38.0	41.0	30.0	41.0
18-19	36.824861754519716	40.0	38.0	41.0	30.0	41.0
20-21	36.88426702457822	40.0	38.0	41.0	30.0	41.0
22-23	36.803783233656674	40.0	38.0	41.0	30.0	41.0
24-25	36.78631015063658	40.0	38.0	41.0	30.0	41.0
26-27	36.7490608301528	40.0	38.0	41.0	30.0	41.0
28-29	36.69987654406911	40.0	38.0	41.0	30.0	41.0
30-31	36.61049038130537	40.0	38.0	41.0	30.0	41.0
32-33	36.526402223277486	40.0	38.0	41.0	30.0	41.0
34-35	36.40026222339527	40.0	37.5	41.0	29.0	41.0
36-37	36.30370399914769	40.0	37.0	41.0	28.0	41.0
38-39	36.18968393461439	40.0	37.0	41.0	28.0	41.0
40-41	36.0808641701015	40.0	37.0	41.0	27.5	41.0
42-43	35.82678051452106	40.0	36.0	41.0	26.5	41.0
44-45	35.4565438870844	40.0	35.0	41.0	26.0	41.0
46-47	35.20650172683047	39.5	35.0	41.0	25.0	41.0
48-49	35.01680708484999	39.0	35.0	41.0	24.0	41.0
50-51	34.81134290179257	39.0	35.0	41.0	24.0	41.0
52-53	34.604817618756734	39.0	34.5	40.0	23.5	41.0
54-55	34.36622190052536	38.0	34.0	40.0	23.0	41.0
56-57	34.1131383129367	38.0	34.0	40.0	22.5	41.0
58-59	34.13000750050993	38.0	34.0	40.0	23.0	41.0
60-61	33.91671382369144	37.5	34.0	40.0	22.0	41.0
62-63	33.60373965467674	37.0	34.0	40.0	22.0	41.0
64-65	33.30280886334349	36.5	33.5	39.5	20.5	41.0
66-67	32.98443684340424	36.0	33.0	39.0	20.0	41.0
68-69	32.64626449442762	35.5	33.0	39.0	19.5	40.5
70-71	32.282023456626874	35.0	33.0	38.0	18.0	40.0
72-73	31.93179247197213	35.0	33.0	37.0	17.5	39.5
74-75	31.564170047963646	35.0	32.0	37.0	14.0	39.0
76-77	31.272741776341327	35.0	32.0	36.0	12.5	39.0
78-79	30.99748323360314	35.0	32.0	36.0	10.5	37.5
80-81	30.742621687877065	35.0	32.0	35.0	7.5	37.0
82-83	30.521762186187175	35.0	31.5	35.0	7.0	37.0
84-85	30.31073231424192	35.0	31.0	35.0	7.0	36.0
86-87	30.14184342297362	35.0	31.0	35.0	3.5	36.0
88-89	29.981739390204794	35.0	31.0	35.0	2.0	36.0
90-91	29.799007480701313	34.0	31.0	35.0	2.0	35.0
92-93	29.719029612826994	34.0	31.0	35.0	2.0	35.0
94-95	29.591746066247545	34.0	31.0	35.0	2.0	35.0
96-97	29.50108652997286	34.0	31.0	35.0	2.0	35.0
98-99	29.40827748995783	34.0	30.5	35.0	2.0	35.0
100-101	29.3005314065785	34.0	30.0	35.0	2.0	35.0
102-103	28.401179576984088	33.5	29.0	34.5	2.0	35.0
104-105	29.339289127258652	34.0	30.5	35.0	2.0	35.0
106-107	29.62409435759283	35.0	31.0	35.0	2.0	35.0
108-109	29.622962588998288	35.0	31.0	35.0	2.0	35.0
110-111	29.55722310885162	35.0	31.0	35.0	2.0	35.0
112-113	29.472657134612472	35.0	31.0	35.0	2.0	35.0
114-115	29.35064991035258	35.0	31.0	35.0	2.0	35.0
116-117	29.230820296668995	35.0	31.0	35.0	2.0	35.0
118-119	28.931719929566945	34.0	31.0	35.0	2.0	35.0
120-121	28.795028355782218	34.0	30.5	35.0	2.0	35.0
122-123	28.54260460962817	34.0	30.0	35.0	2.0	35.0
124-125	27.747683595190892	33.5	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69094.0
3	12701.0
4	6071.0
5	5234.0
6	5695.0
7	6103.0
8	6281.0
9	6749.0
10	7260.0
11	7381.0
12	7783.0
13	7466.0
14	6918.0
15	6337.0
16	5678.0
17	5284.0
18	4988.0
19	4809.0
20	4861.0
21	4984.0
22	5945.0
23	7686.0
24	10732.0
25	13692.0
26	15689.0
27	17897.0
28	21699.0
29	26415.0
30	32247.0
31	40071.0
32	53239.0
33	73508.0
34	114225.0
35	186883.0
36	366126.0
37	546629.0
38	143422.0
39	91.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.79448160418707	17.01602655581396	11.454631229641498	25.734860610357472
2	16.86409541687593	19.732684113642716	37.84040932109282	25.56281114838853
3	20.245199716014653	23.072524417706894	28.47001288214073	28.212262984137727
4	14.29574968650846	14.608812887045596	34.30245321185603	36.792984214589914
5	14.876023322297597	37.047466519309324	32.52816897260556	15.548341185787512
6	34.63383736215213	33.06108953607449	16.784175742204184	15.520897359569197
7	30.44999121487557	31.113321676973115	19.94646430882498	18.490222799326332
8	27.76827038906377	34.97631722862029	18.80936426903714	18.446048113278795
9	28.016680018678535	16.10434704377889	18.078124263675814	37.80084867386675
10-11	26.871833053017234	25.127133169785854	26.31932467031613	21.681709106880778
12-13	26.52479075882604	22.52204183725838	26.495074134945064	24.45809326897052
14-15	24.134675526081296	23.954772217081363	23.60532764411543	28.305224612721915
16-17	24.922941388326937	26.912420554854137	24.25297128527418	23.91166677154475
18-19	24.785823297894076	26.309663988079855	25.547301779264703	23.357210934761365
20-21	25.397909199426135	25.38492269414558	25.282530124675805	23.934637981752488
22-23	25.454183219990593	24.891709395575617	25.119269622182543	24.534837762251243
24-25	24.899126202231713	25.26087681815833	25.171148741624645	24.668848237985312
26-27	24.52202561355144	25.506268145565702	25.355091363534232	24.61661487734863
28-29	24.67172363003381	25.905372744618887	25.16056639775048	24.262337227596824
30-31	25.296639111995773	25.45169605412079	25.153898901588512	24.097765932294926
32-33	24.717565256135217	25.427989850771205	25.25525695992254	24.599187933171034
34-35	24.79950489149952	25.814252365543684	25.26461980043014	24.121622942526656
36-37	24.837607752851103	25.740590030518817	25.189082829148152	24.23271938748193
38-39	24.898527443105756	25.829745649263717	25.188862115127176	24.082864792503347
40-41	24.85518328810726	25.51904650068569	25.28421727474377	24.34155293646328
42-43	24.76534359823172	25.462518832352636	25.11338234274333	24.658755226672312
44-45	24.48136035014334	25.53320396791995	25.488627516494628	24.496808165442086
46-47	24.42932527890148	25.464045959740517	25.476308601975784	24.63032015938222
48-49	24.487101390872905	25.716970952190334	25.39191181097302	24.404015845963738
50-51	24.46317375723212	25.719302717627627	25.51703899186232	24.300484533277924
52-53	24.65568016938937	25.61617981234472	25.389288446292156	24.33885157197375
54-55	24.36309863748049	25.32421720524595	25.60917653100375	24.703507626269808
56-57	24.447195137004034	25.60620774726044	25.234248425023235	24.71234869071229
58-59	24.398045258482785	25.7770359905149	25.421385052112072	24.403533698890236
60-61	24.742576637969766	25.59167613144991	25.52353504700798	24.142212183572347
62-63	24.599950199603203	25.644874976907072	25.533573767507118	24.221601055982607
64-65	24.460469600619657	25.36888604727064	25.491163093804726	24.67948125830497
66-67	24.446911984936996	25.62113875952449	25.3441964616826	24.587752793855913
68-69	24.294065153907386	25.596200850294554	25.542438287512443	24.56729570828562
70-71	24.2540414207633	25.495705567804787	25.705506208990254	24.544746802441658
72-73	24.40021494188831	25.803040083663483	25.388337180314775	24.408407794133435
74-75	24.353300344699456	25.740905727536443	25.37152369152879	24.53427023623531
76-77	24.343103605219618	25.416182525956188	25.468416177171406	24.77229769165279
78-79	24.251088806909674	25.500599493094985	25.632064928395515	24.616246771599826
80-81	24.404604134103757	25.745995130489913	25.32504006609768	24.524360669308646
82-83	24.46359297190793	25.724136435355515	25.39695204446066	24.415318548275895
84-85	24.52724971843131	25.816857069656358	25.15447793939105	24.501415272521278
86-87	24.19149248900237	25.94894465322829	25.293250048258116	24.566312809511224
88-89	24.196005584064316	25.65210336837743	25.404841985652144	24.74704906190611
90-91	24.495725996868025	25.472962281624508	25.469910091584442	24.56140162992303
92-93	24.445147022486456	25.635981109633676	25.549030384299183	24.36984148358069
94-95	24.40532266765185	25.537989128179596	25.475558482549804	24.58112972161875
96-97	24.22906403940887	25.513198757763977	25.55879203255515	24.698945170272008
98-99	24.174138239353745	25.580164540574575	25.378772060012444	24.866925160059235
100-101	24.112988741978395	25.75118843298716	25.480185287658742	24.655637537375704
102-103	23.97689263184946	25.61855442424989	25.674716679092143	24.72983626480851
104-105	24.031049462628108	25.66000319093005	25.583576133128172	24.725371213313675
106-107	24.087623480510135	25.609877209452232	25.566592276543876	24.73590703349376
108-109	24.06668153404581	25.641731323256533	25.593675598963067	24.69791154373459
110-111	23.95147467716216	25.901899483871695	25.591622879680614	24.55500295928553
112-113	23.83929090077306	26.01852573126795	25.61072728873998	24.531456079219012
114-115	23.903411430404155	26.12724234365103	25.514166862547405	24.455179363397413
116-117	23.86527510116976	25.95821823435513	25.48937198856501	24.687134675910098
118-119	23.90937239538245	25.83949717543697	25.554987377041922	24.696143052138655
120-121	23.815349198276092	25.99317397007254	25.64558181866852	24.54589501298284
122-123	23.769611728530393	26.094256220569523	25.540814051194005	24.59531799970608
124-125	23.760070932879067	26.009187762682046	25.548994061358442	24.681747243080444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	462.0
1	368.0
2	251.0
3	200.5
4	167.5
5	148.5
6	146.0
7	157.0
8	163.0
9	178.0
10	189.5
11	220.0
12	251.0
13	268.5
14	296.0
15	314.0
16	344.0
17	409.0
18	453.5
19	517.5
20	604.5
21	685.5
22	808.0
23	990.5
24	1270.0
25	1694.5
26	2346.5
27	3280.0
28	4450.5
29	6122.0
30	8148.0
31	10473.0
32	13636.5
33	16654.0
34	19448.0
35	22776.5
36	26751.0
37	30750.0
38	34434.5
39	38274.5
40	42002.5
41	45688.0
42	49436.5
43	54494.5
44	60198.5
45	65071.5
46	69420.0
47	73477.0
48	78730.0
49	83487.0
50	85440.0
51	85685.0
52	84056.5
53	82089.5
54	81630.0
55	79002.0
56	73400.0
57	67418.5
58	61731.5
59	55450.5
60	47639.0
61	40338.0
62	34129.0
63	26854.5
64	20111.5
65	15598.0
66	12028.0
67	9417.0
68	7470.5
69	5895.5
70	4794.0
71	3776.0
72	2976.0
73	2562.5
74	2104.0
75	1617.0
76	1202.5
77	768.0
78	485.0
79	343.0
80	230.5
81	151.5
82	112.5
83	95.0
84	69.5
85	48.5
86	45.0
87	41.0
88	32.0
89	25.0
90	17.0
91	20.0
92	21.0
93	18.5
94	19.5
95	13.0
96	6.5
97	3.0
98	3.5
99	3.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.021950100461862236
2	0.010439681926983259
3	0.009154797997508396
4	0.010653829248562402
5	0.016275196440014926
6	0.09508141078113982
7	0.0578733136567636
8	0.04839729467688649
9	0.02617951006305033
10-11	0.04994986275833528
12-13	0.012393776236392946
14-15	0.02575121541989204
16-17	0.033487287411938606
18-19	0.041839032953525215
20-21	0.029445256717132266
22-23	0.03613736051648051
24-25	0.030221540757856664
26-27	0.0456936847419498
28-29	0.043391601034974005
30-31	0.026473962630221646
32-33	0.02885635158278962
34-35	0.04323099054378964
36-37	0.009261871658297968
38-39	0.019969237737255157
40-41	0.041303664649577354
42-43	0.021869795216270055
44-45	0.015793364966461852
46-47	0.022083942537849198
48-49	0.027865920220486083
50-51	0.027250246670946044
52-53	0.02460017356640414
54-55	0.02213747936824399
56-57	0.02486785771837807
58-59	0.01667672266797582
60-61	0.02269961608738924
62-63	0.022378395105020526
64-65	0.021013205929953482
66-67	0.02765177289890694
68-69	0.02154857423390134
70-71	0.04617551621550287
72-73	0.020959669099558695
74-75	0.008512356032770965
76-77	0.01622165960962014
78-79	0.024734015642391107
80-81	0.017881301351858503
82-83	0.02272638450258663
84-85	0.011323039628497227
86-87	0.01667672266797582
88-89	0.02227132144423095
90-91	0.019353564187715118
92-93	0.007950219313625712
94-95	0.010734134494154581
96-97	0.014615554697776562
98-99	0.006799177460137815
100-101	0.009770471547048434
102-103	0.003774346542832409
104-105	0.004229409601188089
106-107	9.368945319087539E-4
108-109	0.013357439183499093
110-111	0.0012581155142774695
112-113	0.0020611679701992586
114-115	0.012848839294748627
116-117	0.0051663041330968435
118-119	0.2781506023161104
120-121	0.0012313470990800766
122-123	9.368945319087539E-4
124-125	0.3121732580319968
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1867873.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.11604585052991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.43043217484106	52.85113368801105
2	9.755221099390637	12.509324065811736
3	3.068735168556698	5.902654943109447
4	1.430220586925922	3.6680035491085685
5	0.8182252374183803	2.62306834191888
6	0.5179041825212202	1.9923580987627059
7	0.36737612813563847	1.6488293273154324
8	0.2755927134803754	1.413593204286376
9	0.2027172291103581	1.1697684440698888
>10	1.0627728458770516	11.928537621118254
>50	0.053114607339616274	2.2999730527817572
>100	0.017271056144177895	1.7966849563073781
>500	4.1697025889452904E-4	0.19607070739847718
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0707366078957189E-4
7	0.0	0.0	0.0	0.0	1.0707366078957189E-4
8	0.0	0.0	0.0	0.0	1.0707366078957189E-4
9	0.0	0.0	0.0	5.3536830394785944E-5	1.6061049118435783E-4
10-11	0.0	0.0	0.0	5.3536830394785944E-5	1.6061049118435783E-4
12-13	0.0	0.0	0.0	5.3536830394785944E-5	1.873789063817508E-4
14-15	0.0	0.0	0.0	5.3536830394785944E-5	3.4798939756610866E-4
16-17	0.0	0.0	0.0	5.3536830394785944E-5	3.747578127635016E-4
18-19	0.0	2.6768415197392972E-5	0.0	1.3384207598696486E-4	4.2829464315828755E-4
20-21	0.0	8.030524559217892E-5	0.0	2.1414732157914377E-4	4.2829464315828755E-4
22-23	0.0	1.3384207598696486E-4	0.0	4.5506305835568055E-4	5.085998887504664E-4
24-25	0.0	1.6061049118435783E-4	0.0	6.959787951322173E-4	5.889051343426453E-4
26-27	0.0	1.6061049118435783E-4	0.0	0.0012045786838826835	5.889051343426453E-4
28-29	0.0	1.6061049118435783E-4	0.0	0.003560199221253265	5.889051343426453E-4
30-31	0.0	1.6061049118435783E-4	0.0	0.013866039072249559	6.156735495400383E-4
32-33	0.0	1.6061049118435783E-4	0.0	0.033406982166346424	7.495156255270032E-4
34-35	0.0	1.6061049118435783E-4	0.0	0.0582480714695271	8.030524559217891E-4
36-37	0.0	1.6061049118435783E-4	0.0	0.09352884269969104	8.030524559217891E-4
38-39	0.0	1.6061049118435783E-4	0.0	0.1485379359303336	8.030524559217891E-4
40-41	0.0	1.6061049118435783E-4	0.0	0.211711395796181	8.030524559217891E-4
42-43	0.0	1.6061049118435783E-4	0.0	0.2750454661532128	8.298208711191821E-4
44-45	0.0	1.6061049118435783E-4	0.0	0.3482838501332799	8.565892863165751E-4
46-47	0.0	1.6061049118435783E-4	0.0	0.42674207507683876	9.101261167113611E-4
48-49	0.0	1.6061049118435783E-4	0.0	0.5077968362945446	0.0010171997775009329
50-51	0.0	1.873789063817508E-4	0.0	0.5949012593468613	0.0010171997775009329
52-53	0.0	2.4091573677653675E-4	0.0	0.689286691332869	0.0010707366078957187
54-55	0.0	3.2122098236871566E-4	0.0	0.7956643733273087	0.0012045786838826835
56-57	0.0	4.2829464315828755E-4	0.0	0.9079043382499774	0.0012581155142774698
58-59	0.0	4.2829464315828755E-4	0.0	1.026943480632784	0.0013116523446722555
60-61	0.0	4.2829464315828755E-4	0.0	1.1481508646465794	0.0013384207598696484
62-63	0.0	4.2829464315828755E-4	0.0	1.284509171662099	0.0013384207598696484
64-65	0.0	4.2829464315828755E-4	0.0	1.4198235104849206	0.0013384207598696484
66-67	0.0	4.2829464315828755E-4	0.0	1.5733403716419692	0.0013651891750670413
68-69	0.0	4.2829464315828755E-4	0.0	1.746210796986733	0.0013919575902644344
70-71	0.0	4.2829464315828755E-4	0.0	1.9299759673168357	0.0013919575902644344
72-73	0.0	4.2829464315828755E-4	0.0	2.096127520447054	0.0015525680814487922
74-75	0.0	4.2829464315828755E-4	0.0	2.2790361014908402	0.0015525680814487922
76-77	0.0	4.2829464315828755E-4	0.0	2.49671685387604	0.0015525680814487922
78-79	0.0	4.2829464315828755E-4	0.0	2.7427988947856736	0.0015525680814487922
80-81	0.0	5.353683039478594E-4	0.0	2.9707319501914746	0.001659641742238364
82-83	0.0	5.353683039478594E-4	0.0	3.210469876699326	0.001873789063817508
84-85	0.0	5.353683039478594E-4	0.0	3.466161778664824	0.001873789063817508
86-87	0.0	5.353683039478594E-4	0.0	3.75718798869088	0.001927325894212294
88-89	0.0	5.353683039478594E-4	0.0	4.095032156897177	0.0020343995550018657
90-91	0.0	5.353683039478594E-4	0.0	4.4388456816924915	0.0020343995550018657
92-93	0.0	5.353683039478594E-4	0.0	4.765955715404634	0.0020343995550018657
94-95	0.0	5.353683039478594E-4	0.0	5.110090461182319	0.0020343995550018657
96-97	0.0	5.353683039478594E-4	0.0	5.500695175742676	0.0020343995550018657
98-99	0.0	5.353683039478594E-4	0.0	5.942293721254068	0.0020343995550018657
100-101	0.0	5.353683039478594E-4	0.0	6.404611020128242	0.0020343995550018657
102-103	0.0	5.353683039478594E-4	0.0	6.8746376225792645	0.0020879363853966515
104-105	0.0	5.353683039478594E-4	0.0	7.351972002379177	0.0021414732157914377
106-107	0.0	5.353683039478594E-4	0.0	7.878024897838343	0.0021950100461862235
108-109	0.0	5.353683039478594E-4	0.0	8.442303090199388	0.0022485468765810093
110-111	0.0	5.353683039478594E-4	0.0	9.04170144329941	0.002409157367765367
112-113	0.0	5.353683039478594E-4	0.0	9.630633346057254	0.0024626941981601533
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	9590	0.0	39.84866	1
GTATCAA	3150	0.0	39.305206	1
TACATGG	9815	0.0	38.198193	2
ACATGGG	9950	0.0	37.321068	3
CATGGGG	8445	0.0	36.15016	4
TCAACGC	3500	0.0	33.835888	4
GGTATCA	2055	0.0	33.600323	1
ATGGGGG	5140	0.0	33.34343	5
CAACGCA	3590	0.0	32.82099	5
AACGCAG	3880	0.0	30.672153	6
ATCAACG	3870	0.0	30.600933	3
TATCAAC	4075	0.0	29.791689	2
ACGCAGA	4670	0.0	25.475996	7
GTACCGT	310	1.2732926E-11	24.953281	6
GAGTACA	6425	0.0	24.458403	1
AGTACAT	6435	0.0	24.044588	2
TGGGGGG	4155	0.0	23.62974	6
TATCACG	180	2.1750637E-5	23.142916	2
CGCAGAG	5150	0.0	23.099676	8
ATGGGGA	3405	0.0	21.321789	5
>>END_MODULE