FastQCFastQC Report
Mon 15 Aug 2016
SRR2921585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867873
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.12618630924051047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26900.045.894461
GTATCAA42500.042.660591
GTACATG95300.035.8594741
TCAACGC51800.034.7618454
TACATGG97000.034.672062
ACATGGG96950.034.2584723
CAACGCA52550.034.0059475
ATCAACG54600.032.9818423
AACGCAG55550.032.396066
CATGGGG80050.032.1027034
ATGGGGG50200.032.0380745
TATCAAC56500.031.9791162
ACGCAGA68450.026.1145297
GTATAGG5050.024.8010541
CGCAGAG73550.024.546778
TGGGGGG37250.024.4756666
GCAGAGT82300.021.9379
AGAGTAC74750.020.28668210-11
GAGTACA66950.020.1326081
GAGTACT34900.019.78872312-13
Produced by FastQC (version 0.11.5)