##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921585_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1867873 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.91612652466201 33.0 31.0 34.0 30.0 34.0 2 32.08499185972494 34.0 31.0 34.0 30.0 34.0 3 32.14661168077273 34.0 31.0 34.0 30.0 34.0 4 35.55267194289976 37.0 35.0 37.0 35.0 37.0 5 35.415085500994984 37.0 35.0 37.0 33.0 37.0 6 35.446043173170764 37.0 36.0 37.0 33.0 37.0 7 35.34371822923721 37.0 36.0 37.0 33.0 37.0 8 35.3818498366859 37.0 36.0 37.0 33.0 37.0 9 37.130115377223184 39.0 38.0 39.0 35.0 39.0 10-11 37.08226790579445 39.0 38.0 39.0 34.5 39.0 12-13 37.02245013445775 39.0 38.0 39.0 34.0 39.0 14-15 38.47269782260357 41.0 39.0 41.0 34.0 41.0 16-17 38.478003322495695 41.0 39.0 41.0 34.0 41.0 18-19 38.47948495427687 41.0 39.0 41.0 34.0 41.0 20-21 38.41622958306051 41.0 39.0 41.0 34.0 41.0 22-23 38.42226532531922 41.0 39.0 41.0 34.0 41.0 24-25 38.385280476777595 41.0 39.0 41.0 34.0 41.0 26-27 38.310297327494965 41.0 39.0 41.0 34.0 41.0 28-29 38.24178062427156 41.0 39.0 41.0 34.0 41.0 30-31 38.13761883168716 41.0 38.5 41.0 34.0 41.0 32-33 38.025447661591556 41.0 38.0 41.0 33.5 41.0 34-35 37.89776365952075 41.0 38.0 41.0 33.0 41.0 36-37 37.82587386829833 41.0 38.0 41.0 33.0 41.0 38-39 37.748137587512645 40.5 38.0 41.0 33.0 41.0 40-41 37.63482768903454 40.0 38.0 41.0 33.0 41.0 42-43 37.48259597949111 40.0 38.0 41.0 32.5 41.0 44-45 37.34113561253896 40.0 38.0 41.0 32.0 41.0 46-47 37.155851870014715 40.0 37.0 41.0 32.0 41.0 48-49 36.97852691269696 40.0 37.0 41.0 31.0 41.0 50-51 36.738927378895674 40.0 36.5 41.0 31.0 41.0 52-53 36.61608658618653 40.0 36.0 41.0 31.0 41.0 54-55 36.49157437363247 40.0 35.5 41.0 31.0 41.0 56-57 36.2948155468814 39.0 35.0 41.0 31.0 41.0 58-59 36.10872179211327 39.0 35.0 41.0 30.5 41.0 60-61 35.85929262856736 39.0 35.0 41.0 30.0 41.0 62-63 35.50467055308364 38.0 35.0 41.0 29.5 41.0 64-65 35.21999648798393 37.0 35.0 40.0 29.0 41.0 66-67 34.9190817576998 37.0 35.0 39.5 29.0 41.0 68-69 34.5545427874379 36.0 35.0 39.0 29.0 41.0 70-71 34.184283942216624 36.0 35.0 39.0 29.0 41.0 72-73 33.862621013313 35.0 35.0 38.0 29.0 40.0 74-75 33.53839313486517 35.0 34.0 37.0 29.0 39.0 76-77 33.2135651085486 35.0 34.0 37.0 28.0 39.0 78-79 32.950322907392525 35.0 34.0 36.0 28.0 38.5 80-81 32.69658831194626 35.0 34.0 36.0 27.0 37.0 82-83 32.475544108191514 35.0 34.0 35.5 27.0 37.0 84-85 32.26190645723773 35.0 34.0 35.0 26.5 36.5 86-87 32.1324774221802 35.0 34.0 35.0 26.0 36.0 88-89 32.03746186170045 35.0 34.0 35.0 26.0 36.0 90-91 31.932233347770435 35.0 34.0 35.0 26.0 36.0 92-93 31.855243102716297 35.0 34.0 35.0 26.0 35.0 94-95 31.78588265904588 35.0 34.0 35.0 25.5 35.0 96-97 31.721696550033112 35.0 34.0 35.0 25.0 35.0 98-99 31.64866642432328 35.0 33.5 35.0 25.0 35.0 100-101 31.58109598457711 35.0 33.0 35.0 25.0 35.0 102-103 30.894379596471495 34.0 32.0 35.0 24.0 35.0 104-105 31.411994016723835 35.0 33.0 35.0 25.0 35.0 106-107 31.512333279618048 35.0 33.0 35.0 25.0 35.0 108-109 31.500048183147356 35.0 33.0 35.0 25.0 35.0 110-111 31.431025824560876 35.0 33.0 35.0 24.0 35.0 112-113 31.396975329693184 35.0 33.0 35.0 24.0 35.0 114-115 31.27276934780898 35.0 33.0 35.0 24.0 35.0 116-117 31.45650052225178 35.0 34.0 35.0 25.0 35.0 118-119 31.442052002464834 35.0 34.0 35.0 24.5 35.0 120-121 31.360816554444547 35.0 34.0 35.0 24.0 35.0 122-123 31.225994219093053 35.0 34.0 35.0 23.5 35.0 124-125 30.586962818135923 34.5 32.5 35.0 17.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24405.0 3 6832.0 4 3814.0 5 2951.0 6 3074.0 7 3437.0 8 3929.0 9 4199.0 10 4622.0 11 4977.0 12 5694.0 13 6862.0 14 8963.0 15 8050.0 16 4866.0 17 4299.0 18 4008.0 19 3764.0 20 3654.0 21 3657.0 22 4004.0 23 4958.0 24 6476.0 25 8241.0 26 10470.0 27 12565.0 28 14994.0 29 18426.0 30 22610.0 31 28684.0 32 37300.0 33 51450.0 34 80325.0 35 143536.0 36 330883.0 37 707424.0 38 269010.0 39 460.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.522459199776385 17.41221867431202 10.673896478251393 27.39142564766021 2 16.22361598526644 19.580104344846603 37.0684933707026 27.127786299184354 3 19.272847426058114 23.100222726690948 28.312744904303567 29.314184942947374 4 13.547141581601874 14.669594665250932 34.015138717954905 37.76812503519229 5 14.73799594313535 37.022906077371736 32.6234355688928 15.615662410600113 6 35.038552120366624 33.28436635443521 16.501818314293 15.175263210905172 7 30.755637498834954 31.314807533877488 19.14569488888913 18.783860078398433 8 28.589187860297983 34.10323663613512 18.522349120073528 18.785226383493363 9 28.261590228577887 15.247040955077571 18.194812560349735 38.29655625599481 10-11 27.150709508134895 24.729459594183364 26.16259183079268 21.95723906688906 12-13 26.9498590269741 21.99275623357357 26.64592402214108 24.41146071731125 14-15 24.32365617116286 23.421301934584275 23.992364406477773 28.262677487775097 16-17 24.606026650011973 26.66393849618172 24.65688524704316 24.07314960676315 18-19 24.558441940986345 25.72896778322564 26.45979664684192 23.25279362894609 20-21 25.19372072033237 24.8556917518522 26.251476952314757 23.69911057550068 22-23 24.961539336685732 24.45490525707142 26.124607998372433 24.458947407870415 24-25 24.444589000812996 24.788311921590484 26.159616465184538 24.607482612411985 26-27 24.220018401165213 25.04900775852446 26.316021943269053 24.414951897041277 28-29 24.309048992712235 25.532367699117884 26.104916617945932 24.05366669022394 30-31 24.893164681116247 25.011109159944063 26.136426908687522 23.959299250252165 32-33 24.478397005092884 24.903544673031703 26.176069655577116 24.4419886662983 34-35 24.57316568792609 25.147292939281503 26.270464342513606 24.009077030278807 36-37 24.544593765083278 25.155192957536464 26.186377178383356 24.113836098996895 38-39 24.606302110813353 25.27844410198013 26.160133566437498 23.955120220769025 40-41 24.592538062573198 24.989901288271707 26.245166471473986 24.172394177681113 42-43 24.520466023451082 24.906882578087732 26.001965946087463 24.570685452373723 44-45 24.28538960752143 25.121353732913903 26.089586935530807 24.503669724033863 46-47 24.322553910055053 25.14408762883562 25.91521410096547 24.618144360143848 48-49 24.307292008250204 25.376311848091305 25.878391129825612 24.43800501383288 50-51 24.358878608746302 25.382948918818283 25.914793045765954 24.34337942666946 52-53 24.543921672657948 25.2952921629025 25.744818852227976 24.415967312211585 54-55 24.225244581410152 25.014709952922797 26.018600661854187 24.74144480381286 56-57 24.299250049254333 25.284711455272785 25.6945931592157 24.721445336257183 58-59 24.26340032009954 25.419136855534674 25.927366475957868 24.390096348407923 60-61 24.548449933520587 25.232597638542053 25.974993461809337 24.243958966128023 62-63 24.46645192773829 25.30484923972729 25.93032789847362 24.298370934060802 64-65 24.290891297213463 25.006812561667736 25.959072163899794 24.74322397721901 66-67 24.22441171683599 25.306214557384916 25.79898469221676 24.670389033562337 68-69 24.18495136690894 25.270121379286714 25.900547363191322 24.64437989061302 70-71 24.112506578338035 25.106444603032436 26.125411095936396 24.65563772269314 72-73 24.237182375148468 25.43964823322912 25.747545880149058 24.57562351147335 74-75 24.191985385921743 25.369099705105718 25.758223095983055 24.680691812989483 76-77 24.110492806633573 25.070884319360626 25.841724523404675 24.976898350601125 78-79 24.008488820278302 25.1671624635246 26.004774961406547 24.819573754790554 80-81 24.137909804360362 25.379910732688998 25.71914685848556 24.763032604465078 82-83 24.166933027513604 25.35964068323297 25.724220419356374 24.749205869897047 84-85 24.171641453436344 25.416156829656572 25.526444914447765 24.885756802459316 86-87 23.83194681865416 25.55725148337173 25.7074490610443 24.903352636929814 88-89 23.793920172347978 25.30822887717369 25.759282468764873 25.13856848171346 90-91 23.99888535274811 25.15173149100666 25.88396158053982 24.965421575705403 92-93 24.0081097590682 25.37016702955715 25.832698475752903 24.789024735621748 94-95 23.934415258490883 25.15081144386816 25.82978751882215 25.084985778818808 96-97 23.713042647263922 25.10544903205324 25.90141957672623 25.280088743956608 98-99 23.70343740597539 25.152179336589104 25.765072580308395 25.37931067712711 100-101 23.59587616502835 25.26633234700646 25.913110795005874 25.224680692959318 102-103 23.502784751032067 25.131688769607425 26.022685963709097 25.34284051565141 104-105 23.685684198015604 25.1652815796363 25.845172557234886 25.30386166511321 106-107 23.68531860701365 25.08215964012244 25.872637500451724 25.35988425241219 108-109 23.60674923681328 25.056027702794587 25.89216019051025 25.445062869881884 110-111 23.457609369809433 25.361808882463276 25.884355535163955 25.29622621256334 112-113 23.33990946883206 25.430826103599564 25.896598522912523 25.332665904655848 114-115 23.319304899208888 25.45004398050617 25.841585589598438 25.389065530686505 116-117 23.187091406602597 25.27846320966292 25.902097662488405 25.63234772124608 118-119 23.29009145121348 25.186761190078418 25.905778721953027 25.61736863675507 120-121 23.135341590365677 25.32572696970054 25.986014727313417 25.552916712620366 122-123 23.02298800113928 25.384534254466644 25.816904409575958 25.775573334818112 124-125 22.875701748509414 25.296791049775575 25.87394653982716 25.953560661887852 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 5.5 2 15.0 3 51.5 4 60.5 5 56.0 6 158.0 7 363.5 8 604.5 9 695.0 10 571.5 11 468.0 12 472.5 13 494.5 14 520.0 15 586.5 16 668.5 17 710.0 18 752.5 19 790.0 20 823.5 21 912.5 22 1032.5 23 1211.5 24 1428.0 25 1737.5 26 2352.5 27 3312.5 28 4457.5 29 6121.0 30 8048.5 31 10301.0 32 13428.5 33 16282.5 34 18953.5 35 22191.5 36 26229.5 37 30208.5 38 33564.5 39 37600.5 40 41832.5 41 45322.5 42 48933.5 43 54391.0 44 59841.5 45 64812.5 46 69595.5 47 73541.5 48 79097.0 49 83710.0 50 86045.5 51 86737.5 52 85091.0 53 83456.5 54 82746.0 55 79898.5 56 74009.5 57 67345.5 58 61887.5 59 56063.5 60 48207.0 61 40588.5 62 33811.5 63 26324.0 64 19499.0 65 14993.0 66 11586.0 67 9013.5 68 7067.0 69 5502.5 70 4380.5 71 3454.0 72 2746.0 73 2377.5 74 1885.5 75 1414.5 76 1006.5 77 568.0 78 335.5 79 215.5 80 119.5 81 70.5 82 46.0 83 21.5 84 12.5 85 6.0 86 7.0 87 5.5 88 1.0 89 2.0 90 1.5 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022485468765810094 2 0.0014990312510540064 3 0.030087698681869703 4 0.16692783717094256 5 0.02253900559620488 6 0.0700797109867748 7 0.05348329356439116 8 0.04507801119240976 9 0.03383527680950471 10-11 0.06384267024578223 12-13 0.05495555640024777 14-15 0.058328376715119284 16-17 0.049896325927940494 18-19 0.03629797100766487 20-21 0.02299406865456056 22-23 0.0029712940869106195 24-25 0.004791546320333341 26-27 0.0030248309173054057 28-29 0.004550630583556804 30-31 0.0024091573677653675 32-33 0.009047724336718825 34-35 0.004363251677175054 36-37 0.0062905775713873475 38-39 0.0020611679701992586 40-41 0.0032389782388845493 42-43 0.0037475781276350155 44-45 0.004791546320333341 46-47 0.004604167413951591 48-49 0.0021682416309888306 50-51 0.001739946987830543 52-53 0.0010439681926983258 54-55 0.004818314735530735 56-57 9.101261167113611E-4 58-59 8.833577015139681E-4 60-61 8.030524559217892E-5 62-63 0.0012581155142774695 64-65 0.0 66-67 0.003479893975661086 68-69 4.8183147355307344E-4 70-71 0.0 72-73 0.002382388952567974 74-75 0.004550630583556804 76-77 0.0021950100461862235 78-79 2.4091573677653672E-4 80-81 0.0 82-83 0.00567490402184731 84-85 0.002007631139804473 86-87 0.0 88-89 0.0012848839294748626 90-91 9.368945319087539E-4 92-93 0.0 94-95 0.0032389782388845493 96-97 0.0014722628358566133 98-99 5.889051343426453E-4 100-101 0.0 102-103 0.0011778102686852907 104-105 0.0 106-107 8.29820871119182E-4 108-109 0.002516231028554939 110-111 8.030524559217892E-5 112-113 4.2829464315828755E-4 114-115 0.0 116-117 0.0017131785726331502 118-119 0.002489462613357546 120-121 0.002730378350134083 122-123 0.0015525680814487924 124-125 0.10696658712878232 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1867873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.0012226247051 #Duplication Level Percentage of deduplicated Percentage of total 1 80.13434730162132 48.882931596316375 2 11.081892640119934 13.520180000860737 3 3.4531066298861592 6.319311788295923 4 1.5792972623962496 3.8535625557608366 5 0.9146694017450049 2.7897975901926433 6 0.6038814784760843 2.2102505104473407 7 0.4198428725565911 1.7927649975354214 8 0.2884324622608875 1.4075786274054596 9 0.22686776399601905 1.245528988010119 >10 1.216086447056564 12.954488241125874 >50 0.06041577443226875 2.5009233986247397 >100 0.020454909414757038 2.0433970375699415 >500 4.406600238419349E-4 0.19574598717429356 >1k 2.6439601430516086E-4 0.28353868068036914 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2357 0.12618630924051047 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.3536830394785944E-5 0.0 5 0.0 0.0 0.0 5.3536830394785944E-5 0.0 6 0.0 0.0 0.0 5.3536830394785944E-5 0.0 7 0.0 0.0 0.0 5.3536830394785944E-5 5.3536830394785944E-5 8 0.0 0.0 0.0 1.0707366078957189E-4 5.3536830394785944E-5 9 0.0 0.0 0.0 1.6061049118435783E-4 1.6061049118435783E-4 10-11 0.0 2.6768415197392972E-5 0.0 1.6061049118435783E-4 1.6061049118435783E-4 12-13 0.0 5.3536830394785944E-5 0.0 2.1414732157914377E-4 1.6061049118435783E-4 14-15 0.0 5.3536830394785944E-5 0.0 2.676841519739297E-4 2.4091573677653675E-4 16-17 0.0 5.3536830394785944E-5 0.0 2.676841519739297E-4 2.676841519739297E-4 18-19 0.0 5.3536830394785944E-5 0.0 2.9445256717132266E-4 2.676841519739297E-4 20-21 0.0 5.3536830394785944E-5 0.0 4.2829464315828755E-4 2.676841519739297E-4 22-23 0.0 5.3536830394785944E-5 0.0 5.621367191452524E-4 4.5506305835568055E-4 24-25 0.0 5.3536830394785944E-5 0.0 8.030524559217891E-4 6.692103799348242E-4 26-27 0.0 5.3536830394785944E-5 0.0 0.0013116523446722555 7.495156255270032E-4 28-29 0.0 5.3536830394785944E-5 0.0 0.003747578127635016 7.495156255270032E-4 30-31 0.0 5.3536830394785944E-5 0.0 0.013973112733039132 8.030524559217891E-4 32-33 0.0 5.3536830394785944E-5 0.0 0.033754971563912536 8.030524559217891E-4 34-35 0.0 5.3536830394785944E-5 0.0 0.05872990294308018 8.030524559217891E-4 36-37 0.0 5.3536830394785944E-5 0.0 0.09422482149482325 8.030524559217891E-4 38-39 0.0 5.3536830394785944E-5 0.0 0.1496086725382293 8.030524559217891E-4 40-41 0.0 5.3536830394785944E-5 0.0 0.21377256376638026 8.030524559217891E-4 42-43 0.0 5.3536830394785944E-5 0.0 0.2769995604626224 8.030524559217891E-4 44-45 0.0 5.3536830394785944E-5 0.0 0.3498364182147288 8.030524559217891E-4 46-47 0.0 5.3536830394785944E-5 0.0 0.4283749484038797 8.565892863165751E-4 48-49 0.0 5.3536830394785944E-5 0.0 0.5091620254696116 9.10126116711361E-4 50-51 0.0 5.3536830394785944E-5 0.0 0.5952224803292301 9.10126116711361E-4 52-53 0.0 5.3536830394785944E-5 0.0 0.6894740702392508 0.0010171997775009329 54-55 0.0 5.3536830394785944E-5 0.0 0.7963871205376383 0.0010707366078957189 56-57 0.0 5.3536830394785944E-5 0.0 0.9093230642554392 0.0010707366078957189 58-59 0.0 5.3536830394785944E-5 0.0 1.0294061748309442 0.0010707366078957189 60-61 0.0 5.3536830394785944E-5 0.0 1.1509347798271081 0.0011242734382905047 62-63 0.0 5.3536830394785944E-5 0.0 1.287774918316181 0.0011242734382905047 64-65 0.0 5.3536830394785944E-5 0.0 1.4251771935243993 0.0011242734382905047 66-67 0.0 5.3536830394785944E-5 0.0 1.580487538499673 0.0011242734382905047 68-69 0.0 5.3536830394785944E-5 0.0 1.755338826569044 0.0011242734382905047 70-71 0.0 5.3536830394785944E-5 0.0 1.9418611436644784 0.0011778102686852907 72-73 0.0 5.3536830394785944E-5 0.0 2.111198138203186 0.0014990312510540064 74-75 0.0 5.3536830394785944E-5 0.0 2.2975063079770415 0.0014990312510540064 76-77 0.0 5.3536830394785944E-5 0.0 2.517301765162835 0.0014990312510540064 78-79 0.0 5.3536830394785944E-5 0.0 2.766408636989774 0.0014990312510540064 80-81 0.0 5.3536830394785944E-5 0.0 2.9970453023305117 0.0016061049118435782 82-83 0.0 5.3536830394785944E-5 0.0 3.239647449264484 0.0016596417422383642 84-85 0.0 5.3536830394785944E-5 0.0 3.4991940030184066 0.0016596417422383642 86-87 0.0 5.3536830394785944E-5 0.0 3.7952526751015725 0.0016864101574357573 88-89 0.0 5.3536830394785944E-5 0.0 4.13668381094432 0.0017131785726331502 90-91 0.0 5.3536830394785944E-5 0.0 4.485984860855101 0.0017131785726331502 92-93 0.0 5.3536830394785944E-5 0.0 4.816574788542904 0.0017131785726331502 94-95 0.0 5.3536830394785944E-5 0.0 5.165581385886513 0.0017131785726331502 96-97 0.0 5.3536830394785944E-5 0.0 5.562931741076615 0.001739946987830543 98-99 0.0 5.3536830394785944E-5 0.0 6.009723359136301 0.001766715403027936 100-101 0.0 5.3536830394785944E-5 0.0 6.474717499530215 0.001766715403027936 102-103 0.0 5.3536830394785944E-5 0.0 6.947581553992161 0.001820252233422722 104-105 0.0 5.3536830394785944E-5 0.0 7.429038269732471 0.001820252233422722 106-107 0.0 5.3536830394785944E-5 0.0 7.960311006155129 0.001820252233422722 108-109 0.0 5.3536830394785944E-5 0.0 8.531522218052299 0.001847020648620115 110-111 0.0 5.3536830394785944E-5 0.0 9.138710179974762 0.0019005574790149009 112-113 0.0 5.3536830394785944E-5 0.0 9.738402985641958 0.001927325894212294 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2690 0.0 45.89446 1 GTATCAA 4250 0.0 42.66059 1 GTACATG 9530 0.0 35.859474 1 TCAACGC 5180 0.0 34.761845 4 TACATGG 9700 0.0 34.67206 2 ACATGGG 9695 0.0 34.258472 3 CAACGCA 5255 0.0 34.005947 5 ATCAACG 5460 0.0 32.981842 3 AACGCAG 5555 0.0 32.39606 6 CATGGGG 8005 0.0 32.102703 4 ATGGGGG 5020 0.0 32.038074 5 TATCAAC 5650 0.0 31.979116 2 ACGCAGA 6845 0.0 26.114529 7 GTATAGG 505 0.0 24.801054 1 CGCAGAG 7355 0.0 24.54677 8 TGGGGGG 3725 0.0 24.475666 6 GCAGAGT 8230 0.0 21.937 9 AGAGTAC 7475 0.0 20.286682 10-11 GAGTACA 6695 0.0 20.132608 1 GAGTACT 3490 0.0 19.788723 12-13 >>END_MODULE