FastQCFastQC Report
Mon 15 Aug 2016
SRR2921584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517313
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG89050.038.8357471
GTATCAA31950.038.1920051
TACATGG92900.037.0279772
CATGGGG77400.036.677124
ACATGGG92550.036.653573
GGTATCA22500.035.4496351
CAACGCA36600.032.5179945
ATGGGGG52400.032.3659555
TCAACGC37600.031.814574
AACGCAG39100.030.8923666
ATCAACG39800.030.2055053
TATCAAC41450.029.2902772
TACACCG2302.6557245E-1028.4603165
ATGGGGA24400.027.0712325
ACGCAGA46950.025.846257
GAGTACA61250.024.4897251
TGGGGGG43950.024.3694386
CGCAGAG49800.024.2468558
AGTACAT62500.023.6150842
GTAAGTA9350.022.9144132
Produced by FastQC (version 0.11.5)