##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921584_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1517313
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.996590024602703	31.0	31.0	34.0	26.0	34.0
2	30.165397647024708	31.0	31.0	34.0	26.0	34.0
3	30.27873088808967	31.0	31.0	34.0	26.0	34.0
4	33.811774498735595	37.0	35.0	37.0	30.0	37.0
5	33.87861766161629	37.0	35.0	37.0	30.0	37.0
6	34.111041031085875	37.0	35.0	37.0	30.0	37.0
7	34.13590274386366	37.0	35.0	37.0	30.0	37.0
8	34.10353104468228	37.0	35.0	37.0	30.0	37.0
9	35.779148402472	39.0	37.0	39.0	30.0	39.0
10-11	35.80851511850224	39.0	37.0	39.0	31.0	39.0
12-13	35.73376488568937	39.0	37.0	39.0	30.0	39.0
14-15	37.057726059158526	41.0	38.0	41.0	31.0	41.0
16-17	37.02155191446985	41.0	38.0	41.0	30.5	41.0
18-19	36.984787252201755	41.0	38.0	41.0	30.0	41.0
20-21	37.03621830169517	41.0	38.0	41.0	30.0	41.0
22-23	36.96952639303822	41.0	38.0	41.0	30.0	41.0
24-25	36.95883644310699	41.0	38.0	41.0	30.0	41.0
26-27	36.91967247364255	41.0	38.0	41.0	30.0	41.0
28-29	36.8627330023535	40.0	38.0	41.0	30.0	41.0
30-31	36.772494864276524	40.0	38.0	41.0	30.0	41.0
32-33	36.67797415562906	40.0	38.0	41.0	30.0	41.0
34-35	36.554724700836275	40.0	37.5	41.0	30.0	41.0
36-37	36.450484178280945	40.0	37.0	41.0	29.0	41.0
38-39	36.33594353966518	40.0	37.0	41.0	29.0	41.0
40-41	36.22153174724002	40.0	37.0	41.0	28.0	41.0
42-43	35.987957329832405	40.0	36.0	41.0	27.5	41.0
44-45	35.683200499830946	40.0	35.5	41.0	27.0	41.0
46-47	35.44880390532474	40.0	35.0	41.0	26.0	41.0
48-49	35.26126646248994	39.0	35.0	41.0	26.0	41.0
50-51	35.056466266353745	39.0	35.0	41.0	26.0	41.0
52-53	34.85329493650947	39.0	35.0	41.0	24.5	41.0
54-55	34.613735926601834	38.5	35.0	40.0	24.0	41.0
56-57	34.36194443730463	38.0	34.0	40.0	23.5	41.0
58-59	34.352825685932956	38.0	34.0	40.0	24.0	41.0
60-61	34.125047699452914	37.5	34.0	40.0	23.5	41.0
62-63	33.79711371351857	37.0	34.0	40.0	23.0	41.0
64-65	33.4935679058968	36.5	34.0	39.5	22.0	41.0
66-67	33.16593445123056	36.0	34.0	39.0	22.0	41.0
68-69	32.81954646140908	35.5	33.0	39.0	20.5	40.5
70-71	32.44522949450773	35.0	33.0	37.5	20.0	40.0
72-73	32.104010181155765	35.0	33.0	37.0	19.5	39.0
74-75	31.73935371278042	35.0	33.0	37.0	17.5	39.0
76-77	31.45070595190313	35.0	33.0	36.0	17.0	39.0
78-79	31.179918711564454	35.0	32.0	36.0	15.5	37.0
80-81	30.926527684136364	35.0	32.0	35.0	12.5	37.0
82-83	30.71641019354609	35.0	32.0	35.0	10.5	36.5
84-85	30.5105294688703	35.0	32.0	35.0	7.5	36.0
86-87	30.3419337341735	35.0	32.0	35.0	7.0	36.0
88-89	30.190153910234738	35.0	32.0	35.0	6.0	35.5
90-91	30.00648580747677	35.0	31.0	35.0	2.0	35.0
92-93	29.930341992720024	35.0	31.0	35.0	2.0	35.0
94-95	29.802585227965487	35.0	31.0	35.0	2.0	35.0
96-97	29.70771620621454	34.0	31.0	35.0	2.0	35.0
98-99	29.60911328117534	34.0	31.0	35.0	2.0	35.0
100-101	29.500147958924757	34.0	31.0	35.0	2.0	35.0
102-103	28.636154175176777	33.5	29.0	34.5	2.0	35.0
104-105	29.54568437758063	34.5	31.0	35.0	2.0	35.0
106-107	29.80634285740648	35.0	32.0	35.0	2.0	35.0
108-109	29.796109965445496	35.0	32.0	35.0	2.0	35.0
110-111	29.729039426934325	35.0	32.0	35.0	2.0	35.0
112-113	29.64323379553197	35.0	32.0	35.0	2.0	35.0
114-115	29.518199606804924	35.0	31.0	35.0	2.0	35.0
116-117	29.385416852027234	35.0	31.0	35.0	2.0	35.0
118-119	29.081650259372985	35.0	31.0	35.0	2.0	35.0
120-121	28.936433352907407	35.0	31.0	35.0	2.0	35.0
122-123	28.680564919696856	35.0	31.0	35.0	2.0	35.0
124-125	27.90626126580343	34.5	28.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	52403.0
3	9069.0
4	4808.0
5	4441.0
6	4833.0
7	5271.0
8	5197.0
9	5335.0
10	5878.0
11	6228.0
12	6299.0
13	6323.0
14	6038.0
15	5207.0
16	4352.0
17	3977.0
18	3826.0
19	3748.0
20	3695.0
21	3942.0
22	4676.0
23	6316.0
24	8944.0
25	10956.0
26	12196.0
27	13900.0
28	16624.0
29	19624.0
30	24309.0
31	30648.0
32	40041.0
33	55865.0
34	91025.0
35	150453.0
36	303208.0
37	463848.0
38	113763.0
39	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.315768656495045	16.93242757743257	11.26400411347658	25.48779965259581
2	17.569781881362236	19.23882580301564	37.319695535594235	25.871696780027893
3	21.035112789091254	23.07087942798688	27.706555898885295	28.187451884036573
4	14.092674653974507	14.942883300805388	33.49099664701807	37.47344539820203
5	15.269094195327707	37.68248157139646	31.990592398114526	15.05783183516131
6	35.26235870861521	32.80308387246573	16.641974754883854	15.292582664035208
7	30.820568718566538	31.761816871381875	18.66907748581105	18.748536924240536
8	28.58612299800212	34.011974073756605	18.39998417523292	19.001918753008354
9	28.68688733165011	15.824724937867932	17.649660828119952	37.838726902362005
10-11	27.34819871975777	25.188119647524577	25.49370692633728	21.969974706380373
12-13	26.817871572174862	22.432404196227797	26.004029333123285	24.745694898474056
14-15	24.59901826222129	23.936813320834176	23.061212919489854	28.40295549745468
16-17	25.280794614228597	26.943624834068043	23.85810629732172	23.917474254381645
18-19	25.09266829300455	26.368596294586933	25.132953121909406	23.40578229049911
20-21	25.581490403315232	25.24283429517733	25.19691761631456	23.978757685192875
22-23	25.7941982462022	24.594045684715272	24.96948275788182	24.64227331120071
24-25	25.30099433199448	24.992360800937472	24.89762484363128	24.809020023436766
26-27	24.735314334317405	25.376131394200733	25.16504220629905	24.723512065182813
28-29	24.796221430203307	25.917765930827542	24.884870259191505	24.40114237977765
30-31	25.63743200478072	25.163875771172826	24.89952545492481	24.29916676912164
32-33	25.03911780168997	25.194567182578858	24.983741431907667	24.78257358382351
34-35	25.092570858904605	25.608765484520095	25.172383554562742	24.126280102012558
36-37	25.115173278151666	25.61847860869955	25.12291795813325	24.143430155015537
38-39	25.22443805615121	25.671336595449866	24.961750442152365	24.142474906246562
40-41	25.09419314002957	25.33336410193859	25.06587503440864	24.506567723623203
42-43	24.84142899143433	25.347597143282798	24.855667751712936	24.955306113569932
44-45	24.576338220162405	25.441591065942198	25.25791475810954	24.724155955785854
46-47	24.56335047817123	25.28699337992818	25.262273171487383	24.887382970413206
48-49	24.589907801154272	25.682344305054205	25.17848260694865	24.549265286842868
50-51	24.630866134153607	25.564780171079683	25.307511295273798	24.496842399492913
52-53	24.847325426249476	25.390157368196224	25.194304104546482	24.56821310100781
54-55	24.29691560811918	25.132195521815927	25.521357779767843	25.04953109029705
56-57	24.520733838147283	25.435444056855676	24.96202029298019	25.081801812016845
58-59	24.573878617532905	25.633298968528518	25.198245812942965	24.594576600995612
60-61	25.009492519367377	25.46065087041129	25.352343861240463	24.177512748980874
62-63	24.73540417545741	25.442695981861373	25.351099731803277	24.470800110877935
64-65	24.47758134193888	25.156517127645795	25.396694081403083	24.96920744901225
66-67	24.5992787875192	25.479863404729414	25.0829658050353	24.83789200271608
68-69	24.427927010388384	25.557900121491144	25.274244998131156	24.739927869989316
70-71	24.323998211834333	25.452415144549985	25.440151176483777	24.783435467131905
72-73	24.467564759735055	25.932470309665444	25.044825549508502	24.555139381091003
74-75	24.374509365799064	25.76644394922385	25.182164940095664	24.67688174488142
76-77	24.397358859698155	25.340128879842222	25.163967168334793	25.09854509212483
78-79	24.29304154387274	25.43184842365066	25.384779936694862	24.890330095781735
80-81	24.603881392164837	25.679467750536737	25.017616624028616	24.69903423326981
82-83	24.63086693081225	25.727792934122146	25.075405422598323	24.565934712467282
84-85	24.632893820989022	25.730290494721636	24.78943878465741	24.847376899631936
86-87	24.233351658275424	26.06957018123236	24.965624254313283	24.73145390617893
88-89	24.168587421797827	25.60967289317635	25.235640645584667	24.986099039441154
90-91	24.666845526804632	25.244921932164104	25.31519155393424	24.77304098709702
92-93	24.779420754766104	25.41074882423098	25.20418389551532	24.6056465254876
94-95	24.631496798934318	25.424595178726445	25.11701208383345	24.826895938505793
96-97	24.368728981252367	25.31003270718174	25.297970203638787	25.02326810792711
98-99	24.112703422902797	25.412184513660176	25.142283161121547	25.332828902315484
100-101	24.182979611112632	25.740875555184985	25.181183838773986	24.8949609949284
102-103	24.07009391992091	25.65384742132147	25.32512769813808	24.95093096061954
104-105	24.14872301861921	25.61535673092766	25.268446202010214	24.967474048442906
106-107	24.182891738098284	25.517925897415278	25.295392743171764	25.003789621314677
108-109	24.22003714305018	25.53382011492264	25.299294875280758	24.946847866746424
110-111	23.969875277797552	26.085025163251373	25.25980496832515	24.68529459062593
112-113	23.85375247894748	26.162658499403868	25.33729833115509	24.64629069049356
114-115	23.92076550457216	26.318174537626298	25.118992141014836	24.64206781678671
116-117	23.83529600963861	26.19640647996251	25.100858076324567	24.86743943407431
118-119	23.801912445967304	26.02263515980724	25.277206191177847	24.89824620304761
120-121	23.877915994831607	26.218258161951546	25.34469957974678	24.559126263470066
122-123	23.802682071248388	26.29227293922253	25.217401333621126	24.687643655907955
124-125	23.707362481959237	26.154195539047713	25.163647807307804	24.974794171685243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	753.0
1	645.0
2	439.0
3	328.5
4	264.0
5	210.0
6	222.0
7	233.5
8	285.0
9	318.5
10	297.0
11	291.0
12	306.5
13	341.5
14	392.0
15	434.5
16	455.5
17	507.5
18	561.5
19	613.5
20	664.5
21	736.0
22	821.0
23	935.0
24	1150.0
25	1450.5
26	1923.5
27	2536.0
28	3290.0
29	4390.0
30	5783.0
31	7448.0
32	9914.5
33	12136.5
34	14175.5
35	16686.0
36	19612.0
37	22681.5
38	25493.0
39	28582.0
40	31644.5
41	34309.0
42	36825.0
43	40981.5
44	45773.5
45	49707.5
46	53207.0
47	57522.5
48	62931.0
49	67288.0
50	70516.0
51	71840.0
52	70599.0
53	69670.0
54	69673.0
55	67398.5
56	62786.0
57	57755.0
58	52712.0
59	46940.5
60	40054.5
61	34325.5
62	29389.0
63	23077.5
64	17477.0
65	13765.0
66	10713.5
67	8377.0
68	6790.5
69	5460.0
70	4453.5
71	3561.5
72	2730.5
73	2295.0
74	1899.5
75	1508.0
76	1087.0
77	676.0
78	456.5
79	308.5
80	222.0
81	150.5
82	100.0
83	82.5
84	60.0
85	47.0
86	48.5
87	37.5
88	24.5
89	20.5
90	18.5
91	18.0
92	17.5
93	10.5
94	7.0
95	8.0
96	6.0
97	3.0
98	3.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02293528098684978
2	0.01034723883602131
3	0.009556367077854075
4	0.010281332856174039
5	0.015949247123039214
6	0.08548005586190852
7	0.0537792795553719
8	0.046331903832630444
9	0.02524199028150421
10-11	0.047781835389270375
12-13	0.012423277201210298
14-15	0.027087357717227756
16-17	0.03305184889340564
18-19	0.040400365646376196
20-21	0.028669101233562228
22-23	0.03608352396638004
24-25	0.030679233618903946
26-27	0.04320136978988515
28-29	0.043135463810037876
30-31	0.02547266121096966
32-33	0.027713464525776818
34-35	0.04198210916271066
36-37	0.009391602128235902
38-39	0.019903605913875383
40-41	0.04056513059599437
42-43	0.021551255410057122
44-45	0.015949247123039214
46-47	0.022111456238758914
48-49	0.028240712364554974
50-51	0.02751574658623501
52-53	0.023792058724864285
54-55	0.02217736221860618
56-57	0.025340849251275117
58-59	0.01693783682074826
60-61	0.02168306736975166
62-63	0.021716020359675296
64-65	0.020200182823188098
66-67	0.02787822947539499
68-69	0.021748973349598928
70-71	0.044486536396906896
72-73	0.021155819530973504
74-75	0.009490461098006804
76-77	0.015883341143191943
78-79	0.024681789452802423
80-81	0.018453674357235456
82-83	0.022836422017078875
84-85	0.011665358432966698
86-87	0.016806024861053717
88-89	0.021847832319369834
90-91	0.019705887974333574
92-93	0.007546234692512356
94-95	0.010676768735257657
96-97	0.01416978566716294
98-99	0.0060633501459487925
100-101	0.009127978208846824
102-103	0.004152076730377977
104-105	0.004152076730377977
106-107	7.249657783199643E-4
108-109	0.013543678858613878
110-111	0.001384025576792659
112-113	0.002372615274501701
114-115	0.012917572050064819
116-117	0.005404290347476097
118-119	0.2784198118647899
120-121	8.89730727938138E-4
122-123	0.001021542687632677
124-125	0.3141078999520863
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1517313.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.01010309303503
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.45718491369813	49.89158331073804
2	10.709320961641794	13.281721937756174
3	3.478906808267939	6.471821094952691
4	1.6434668588361006	4.076461973856521
5	0.9617744304822422	2.9819865793224447
6	0.5958619626473826	2.216967703978943
7	0.4116560388064687	1.7868783383681686
8	0.3106783733989841	1.5412158370597946
9	0.2257248987730768	1.2597501819225052
>10	1.137552197686679	12.292798849871254
>50	0.049329138806123024	2.0821211823345105
>100	0.018013648327172182	1.846329220277871
>500	4.238149023916769E-4	0.18771065535273557
>1k	1.0595372559791923E-4	0.08265313420851991
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	6.590597984726948E-5	0.0	0.0	0.0	0.0
12-13	6.590597984726948E-5	0.0	0.0	0.0	6.590597984726948E-5
14-15	6.590597984726948E-5	0.0	0.0	0.0	2.3067092946544318E-4
16-17	6.590597984726948E-5	0.0	0.0	3.295298992363474E-5	2.6362391938907794E-4
18-19	6.590597984726948E-5	0.0	0.0	6.590597984726948E-5	2.965769093127127E-4
20-21	6.590597984726948E-5	0.0	0.0	6.590597984726948E-5	3.295298992363474E-4
22-23	6.590597984726948E-5	0.0	0.0	3.6248288915998215E-4	3.9543587908361685E-4
24-25	6.590597984726948E-5	0.0	0.0	9.885896977090422E-4	4.6134185893088636E-4
26-27	6.590597984726948E-5	0.0	0.0	0.001647649496181737	4.6134185893088636E-4
28-29	6.590597984726948E-5	0.0	0.0	0.005766773236636079	4.6134185893088636E-4
30-31	6.590597984726948E-5	0.0	0.0	0.020958101591431695	4.6134185893088636E-4
32-33	6.590597984726948E-5	0.0	0.0	0.04804545930865946	6.590597984726948E-4
34-35	6.590597984726948E-5	0.0	0.0	0.08676522246893027	7.908717581672338E-4
36-37	6.590597984726948E-5	0.0	0.0	0.1416319506917821	7.908717581672338E-4
38-39	6.590597984726948E-5	0.0	0.0	0.22536549808773798	7.908717581672338E-4
40-41	6.590597984726948E-5	0.0	0.0	0.324784668687344	8.567777380145033E-4
42-43	6.590597984726948E-5	0.0	0.0	0.41939270275809937	8.567777380145033E-4
44-45	6.590597984726948E-5	0.0	0.0	0.5306419967402902	8.567777380145033E-4
46-47	6.590597984726948E-5	0.0	0.0	0.6458786025032409	9.226837178617727E-4
48-49	6.590597984726948E-5	0.0	0.0	0.7658274858252714	0.0010544956775563117
50-51	6.590597984726948E-5	0.0	0.0	0.8858093221372255	0.0010544956775563117
52-53	6.590597984726948E-5	0.0	0.0	1.0112284017865794	0.0011863076372508508
54-55	6.590597984726948E-5	0.0	0.0	1.161559941818201	0.0013181195969453896
56-57	6.590597984726948E-5	0.0	0.0	1.322041002746302	0.0013181195969453896
58-59	6.590597984726948E-5	0.0	0.0	1.4836754183217304	0.0013181195969453896
60-61	6.590597984726948E-5	0.0	0.0	1.650549359295017	0.0013181195969453896
62-63	6.590597984726948E-5	0.0	0.0	1.8310987910866117	0.001384025576792659
64-65	6.590597984726948E-5	0.0	0.0	2.0158002996085846	0.001384025576792659
66-67	6.590597984726948E-5	0.0	0.0	2.2313458066990792	0.001384025576792659
68-69	6.590597984726948E-5	0.0	0.0	2.462214454104064	0.001384025576792659
70-71	6.590597984726948E-5	0.0	0.0	2.7027053745667504	0.001384025576792659
72-73	6.590597984726948E-5	0.0	0.0	2.9308718767979975	0.0015817435163344676
74-75	6.590597984726948E-5	0.0	0.0	3.174855814192589	0.0016476494961817371
76-77	6.590597984726948E-5	0.0	0.0	3.4609207197196623	0.0016806024861053719
78-79	6.590597984726948E-5	0.0	0.0	3.7733150641957196	0.0017135554760290066
80-81	6.590597984726948E-5	0.0	0.0	4.08165619091117	0.0017465084659526414
82-83	6.590597984726948E-5	0.0	0.0	4.39362214651822	0.0018783204256471802
84-85	6.590597984726948E-5	0.0	0.0	4.719395404903272	0.001911273415570815
86-87	6.590597984726948E-5	3.295298992363474E-5	0.0	5.09334593455668	0.001911273415570815
88-89	6.590597984726948E-5	6.590597984726948E-5	0.0	5.515045346609433	0.001911273415570815
90-91	6.590597984726948E-5	6.590597984726948E-5	0.0	5.941061600342184	0.001911273415570815
92-93	6.590597984726948E-5	6.590597984726948E-5	0.0	6.363650743122875	0.0019771793954180845
94-95	6.590597984726948E-5	6.590597984726948E-5	0.0	6.793489543686768	0.002043085375265354
96-97	6.590597984726948E-5	6.590597984726948E-5	0.0	7.275525880289696	0.002076038365188989
98-99	6.590597984726948E-5	6.590597984726948E-5	0.0	7.822084171163102	0.002174897334959893
100-101	6.590597984726948E-5	6.590597984726948E-5	0.0	8.388084726091453	0.002174897334959893
102-103	6.590597984726948E-5	6.590597984726948E-5	0.0	8.958764605588959	0.002174897334959893
104-105	6.590597984726948E-5	6.590597984726948E-5	0.0	9.559266940967355	0.002174897334959893
106-107	6.590597984726948E-5	6.590597984726948E-5	0.0	10.212461107233644	0.002174897334959893
108-109	6.590597984726948E-5	6.590597984726948E-5	0.0	10.899596853121274	0.002174897334959893
110-111	6.590597984726948E-5	6.590597984726948E-5	0.0	11.639391476906873	0.0022408033148071625
112-113	6.590597984726948E-5	6.590597984726948E-5	0.0	12.349660221720898	0.002306709294654432
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	8905	0.0	38.835747	1
GTATCAA	3195	0.0	38.192005	1
TACATGG	9290	0.0	37.027977	2
CATGGGG	7740	0.0	36.67712	4
ACATGGG	9255	0.0	36.65357	3
GGTATCA	2250	0.0	35.449635	1
CAACGCA	3660	0.0	32.517994	5
ATGGGGG	5240	0.0	32.365955	5
TCAACGC	3760	0.0	31.81457	4
AACGCAG	3910	0.0	30.892366	6
ATCAACG	3980	0.0	30.205505	3
TATCAAC	4145	0.0	29.290277	2
TACACCG	230	2.6557245E-10	28.460316	5
ATGGGGA	2440	0.0	27.071232	5
ACGCAGA	4695	0.0	25.84625	7
GAGTACA	6125	0.0	24.489725	1
TGGGGGG	4395	0.0	24.369438	6
CGCAGAG	4980	0.0	24.246855	8
AGTACAT	6250	0.0	23.615084	2
GTAAGTA	935	0.0	22.914413	2
>>END_MODULE