FastQCFastQC Report
Mon 15 Aug 2016
SRR2921584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1517313
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29650.195411230247154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23430.15441771078215238No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27400.046.9983371
GTATCAA44700.044.813731
GTACATG91500.037.3347131
ATGGGGG51400.036.6186075
TACATGG94000.035.7016372
CATGGGG78300.035.3176734
ACATGGG94350.035.2521483
TCAACGC57200.034.5921824
CAACGCA57700.034.272055
CGTATAC700.002764929334.060833
TATCAAC60250.033.143272
ATCAACG60050.032.9547733
AACGCAG61100.032.4817126
TGGGGGG39750.029.8367716
ACGCAGA73750.026.7459987
CGCAGAG77600.025.41828
GCAGAGT83300.023.67899
GAGTACT36550.022.55816812-13
AGAGTAC78350.022.0185210-11
GTACTTT38000.021.53221514-15
Produced by FastQC (version 0.11.5)