##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2921584_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1517313 Sequences flagged as poor quality 0 Sequence length 125 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836014059063622 33.0 31.0 34.0 30.0 34.0 2 31.995671295243632 34.0 31.0 34.0 30.0 34.0 3 32.05744958357307 34.0 31.0 34.0 30.0 34.0 4 35.45809005788522 37.0 35.0 37.0 35.0 37.0 5 35.32190062300923 37.0 35.0 37.0 33.0 37.0 6 35.34460127870782 37.0 35.0 37.0 33.0 37.0 7 35.23859019200389 37.0 35.0 37.0 33.0 37.0 8 35.27261349504025 37.0 36.0 37.0 33.0 37.0 9 37.00329859429136 39.0 38.0 39.0 34.0 39.0 10-11 36.9519268601798 39.0 38.0 39.0 34.0 39.0 12-13 36.879790788057576 39.0 38.0 39.0 34.0 39.0 14-15 38.31098395650733 41.0 39.0 41.0 34.0 41.0 16-17 38.310459015377845 41.0 39.0 41.0 34.0 41.0 18-19 38.320059209932296 41.0 39.0 41.0 34.0 41.0 20-21 38.25434666413588 41.0 39.0 41.0 34.0 41.0 22-23 38.25984783627373 41.0 39.0 41.0 34.0 41.0 24-25 38.21422211501516 41.0 39.0 41.0 34.0 41.0 26-27 38.133140624248256 41.0 39.0 41.0 34.0 41.0 28-29 38.05920037592771 41.0 39.0 41.0 34.0 41.0 30-31 37.93841910008021 41.0 38.0 41.0 33.0 41.0 32-33 37.81108611077609 41.0 38.0 41.0 33.0 41.0 34-35 37.67545325189991 41.0 38.0 41.0 33.0 41.0 36-37 37.59839532120268 40.0 38.0 41.0 33.0 41.0 38-39 37.51081615988264 40.0 38.0 41.0 33.0 41.0 40-41 37.37920817919572 40.0 38.0 41.0 32.0 41.0 42-43 37.21381415699991 40.0 37.0 41.0 32.0 41.0 44-45 37.0539367948472 40.0 37.0 41.0 31.5 41.0 46-47 36.834986255307896 40.0 37.0 41.0 31.0 41.0 48-49 36.625836264501785 40.0 36.0 41.0 30.5 41.0 50-51 36.36756852409489 40.0 36.0 41.0 30.0 41.0 52-53 36.23207703354549 40.0 35.0 41.0 30.0 41.0 54-55 36.096672868419375 39.5 35.0 41.0 30.0 41.0 56-57 35.8998024797784 39.0 35.0 41.0 29.0 41.0 58-59 35.70269746584917 39.0 35.0 41.0 29.0 41.0 60-61 35.442757361203654 38.5 35.0 41.0 29.0 41.0 62-63 35.07985333283245 37.5 35.0 40.5 28.5 41.0 64-65 34.79534545607927 37.0 35.0 40.0 28.0 41.0 66-67 34.48853433668597 36.5 35.0 39.5 28.0 41.0 68-69 34.12135103304328 36.0 35.0 39.0 28.0 41.0 70-71 33.74625802322922 35.5 34.5 39.0 27.5 40.5 72-73 33.43709735565437 35.0 34.0 37.0 27.0 39.5 74-75 33.122000206944776 35.0 34.0 37.0 27.0 39.0 76-77 32.8052557382689 35.0 34.0 36.5 26.0 39.0 78-79 32.55141424346856 35.0 34.0 36.0 26.0 37.5 80-81 32.31050383144414 35.0 34.0 36.0 26.0 37.0 82-83 32.09942312495839 35.0 34.0 35.0 26.0 37.0 84-85 31.900234823006194 35.0 34.0 35.0 25.5 36.0 86-87 31.78195764486299 35.0 34.0 35.0 25.0 36.0 88-89 31.69349699106249 35.0 34.0 35.0 25.0 36.0 90-91 31.59561606603252 35.0 34.0 35.0 25.0 35.5 92-93 31.519377346664797 35.0 33.0 35.0 25.0 35.0 94-95 31.44963761596981 35.0 33.0 35.0 24.0 35.0 96-97 31.38873389999295 35.0 33.0 35.0 24.0 35.0 98-99 31.318026669513806 35.0 33.0 35.0 24.0 35.0 100-101 31.249865057506263 35.0 33.0 35.0 23.5 35.0 102-103 30.551116677969542 34.0 31.5 35.0 21.5 35.0 104-105 31.066913352749236 35.0 33.0 35.0 23.0 35.0 106-107 31.164272302418816 35.0 33.0 35.0 23.0 35.0 108-109 31.145731632168182 35.0 33.0 35.0 23.0 35.0 110-111 31.077061225996218 35.0 33.0 35.0 20.5 35.0 112-113 31.030471629782387 35.0 33.0 35.0 20.0 35.0 114-115 30.89409403333393 35.0 33.0 35.0 19.0 35.0 116-117 31.072940784136165 35.0 33.0 35.0 20.0 35.0 118-119 31.046600470700508 35.0 33.0 35.0 19.5 35.0 120-121 30.95672679269208 35.0 33.0 35.0 18.0 35.0 122-123 30.810366417476157 35.0 33.0 35.0 6.0 35.0 124-125 30.15606173544944 34.5 32.0 35.0 2.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21945.0 3 6417.0 4 3420.0 5 2814.0 6 2955.0 7 3233.0 8 3586.0 9 3857.0 10 4129.0 11 4454.0 12 5084.0 13 6516.0 14 8649.0 15 8262.0 16 4679.0 17 4148.0 18 3763.0 19 3183.0 20 3242.0 21 3400.0 22 3611.0 23 4390.0 24 5766.0 25 7394.0 26 8853.0 27 10550.0 28 12924.0 29 15557.0 30 18735.0 31 23999.0 32 31457.0 33 42854.0 34 67552.0 35 123388.0 36 273790.0 37 562815.0 38 195677.0 39 265.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.94347209861894 17.14974125712779 10.582352747288969 27.3244338969643 2 16.46095959432856 19.368056274126122 36.63618023485339 27.534803896691923 3 19.49885386951715 22.861232177621545 28.1949417309504 29.444972221910902 4 13.761544809374485 14.283941244429773 33.457996369037794 38.496517577157945 5 14.728380673128646 37.439748552383165 32.252520804548 15.579349969940196 6 35.459731948605565 32.776777935979204 16.195722494330138 15.56776762108509 7 31.071578816879715 31.731358872610887 18.522438192600916 18.674624117908486 8 29.375877331501254 33.630914508269086 18.130078138135246 18.863130022094413 9 28.885853316359327 14.796658028030846 17.877559039285245 38.439929616324584 10-11 27.714019861343843 24.544929382694555 25.44165079038494 22.29939996557666 12-13 27.363976675592788 21.701583160954367 26.295347976918254 24.63909218653459 14-15 24.79333801960585 23.12398531869071 23.646476129684018 28.436200532019416 16-17 24.91368839995833 26.375908447723127 24.46465708117774 24.245746071140804 18-19 24.810537259961666 25.5382917465778 26.344303657265446 23.30686733619508 20-21 25.354588890470993 24.412182722377796 26.463676227843962 23.76955215930725 22-23 25.249707534889854 23.901435138653177 26.273351897315912 24.57550542914106 24-25 24.800865248773853 24.25814045990398 26.254183963568334 24.686810327753836 26-27 24.380372954335222 24.63069203206958 26.51665317197705 24.47228184161815 28-29 24.3563498314379 25.25940108552014 26.136022857218183 24.248226225823778 30-31 25.229431414761976 24.513349021458417 26.16729174211675 24.08992782166286 32-33 24.743456484327268 24.397652500776108 26.245411745010017 24.61347926988661 34-35 24.731990354915258 24.809828574667513 26.427690369975405 24.03049070044182 36-37 24.630885709521337 24.86022068094592 26.530025777527612 23.97886783200513 38-39 24.880411313900364 24.929874697156233 26.23658458139676 23.95312940754664 40-41 24.862960836820413 24.57962243955744 26.23068319550877 24.32673352811338 42-43 24.5659546295921 24.562033103611 26.061143510385286 24.810868756411615 44-45 24.329303999971 24.769311025847117 26.22975076264699 24.671634211534894 46-47 24.371106230196457 24.69854127293009 26.061700546548934 24.86865195032452 48-49 24.426780589414452 25.118015034085545 25.91453618979421 24.540668186705798 50-51 24.575214444125894 25.037385610576717 25.963111985474153 24.42428795982324 52-53 24.72579654439717 24.976504378819275 25.70289144429857 24.594807632484986 54-55 24.08834582749489 24.66640127283584 26.185341560542092 25.05991133912718 56-57 24.37800059249808 24.84804467395964 25.633285869777623 25.140668863764652 58-59 24.405145966038468 25.04410780957026 25.878514866819792 24.672231357571484 60-61 24.874778555761768 24.845582187447278 26.005923633372703 24.273715623418255 62-63 24.610508541823332 24.858416172422178 25.987494884001112 24.543580401753378 64-65 24.24377831073747 24.480117154469777 26.126679201984032 25.149425332808722 66-67 24.402927257190857 24.876347539127366 25.75157858938156 24.969146614300218 68-69 24.221232306575953 24.9948098630397 25.79633177596271 24.987626054421636 70-71 24.097631800426146 24.828627975902137 26.060245974297985 25.01349424937373 72-73 24.327679363493402 25.21663738184464 25.587432000115996 24.86825125454596 74-75 24.17605268517353 25.134075778437015 25.713747142012565 24.976124394376892 76-77 24.017361193494725 24.604360927230882 25.810654771790798 25.567623107483595 78-79 23.88491878065868 24.748584831982367 25.999152447981988 25.367343939376962 80-81 24.142744443631603 25.036396577370656 25.599398410215958 25.22146056878179 82-83 24.20611211531522 25.074238786738356 25.563847333879288 25.155801764067142 84-85 24.128266590566223 24.99906082740776 25.431277940384618 25.441394641641395 86-87 23.61407962628673 25.360555139249453 25.55632885238576 25.469036382078052 88-89 23.60325038003374 24.931037016752462 25.731867703113327 25.733844900100472 90-91 23.959355567931297 24.62296637788711 25.88480892108047 25.532869133101123 92-93 24.034362059772768 24.76954326496906 25.741985997615522 25.45410867764265 94-95 23.970603839915427 24.635678615516127 25.692212278212295 25.701505266356158 96-97 23.547910229777184 24.59315782813637 25.875307125307124 25.983624816779322 98-99 23.393778302685753 24.649625025456352 25.75299725895223 26.203599412905664 100-101 23.42858724600659 24.861515059845924 25.804893255379742 25.905004438767744 102-103 23.385841354202093 24.70734044600512 25.914754707729298 25.992063492063494 104-105 23.548008881489842 24.682810995490055 25.737965732844835 26.03121439017526 106-107 23.478000969481755 24.57069456348771 25.847662188003667 26.103642279026868 108-109 23.50407423286217 24.505241123998577 25.770894551728933 26.219790091410317 110-111 23.259262530156175 25.02910579548866 25.77885395168224 25.932777722672927 112-113 23.16087993970903 25.09812605676433 25.73464789790606 26.006346105620576 114-115 23.129802486368995 25.130312598653013 25.60931726018297 26.130567654795023 116-117 22.878502381550252 24.941952390085213 25.705650011303057 26.473895217061482 118-119 22.914273398990428 24.750358701805933 25.81430417781328 26.52106372139036 120-121 22.88331472544913 24.87250194097588 25.892717089419342 26.351466244155652 122-123 22.785258541658564 24.967063182668674 25.660565254442268 26.58711302123049 124-125 22.460371075040968 24.85944826931044 25.654203819471018 27.025976836177573 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 42.0 1 33.0 2 42.0 3 83.0 4 84.0 5 87.0 6 236.5 7 543.0 8 870.5 9 1006.0 10 824.5 11 618.0 12 598.5 13 613.5 14 672.5 15 751.0 16 785.5 17 827.5 18 837.0 19 852.0 20 901.5 21 966.5 22 1059.5 23 1200.5 24 1349.5 25 1554.5 26 1961.0 27 2540.0 28 3268.0 29 4279.5 30 5620.5 31 7211.0 32 9657.5 33 11960.5 34 13895.0 35 16317.5 36 19280.0 37 22425.0 38 25006.0 39 27990.0 40 31109.5 41 33660.5 42 36470.0 43 40651.0 44 44968.5 45 48823.0 46 52692.5 47 56925.5 48 62045.5 49 66843.5 50 70704.5 51 72650.5 52 71811.5 53 70634.0 54 70471.5 55 68430.5 56 64084.5 57 58889.0 58 53419.0 59 47327.0 60 40473.5 61 34879.0 62 29527.0 63 22967.5 64 17371.5 65 13305.0 66 10161.5 67 8035.5 68 6509.0 69 5151.0 70 4167.0 71 3271.0 72 2480.5 73 2131.0 74 1723.5 75 1324.5 76 950.0 77 566.5 78 333.0 79 218.5 80 147.5 81 75.5 82 43.0 83 23.0 84 12.0 85 7.5 86 4.0 87 3.5 88 3.0 89 1.5 90 1.5 91 1.0 92 1.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02392387068455882 2 0.0017135554760290064 3 0.030975810528216654 4 0.16891702634855169 5 0.022210315208529816 6 0.06340155261307324 7 0.0492317669459103 8 0.04217982710225247 9 0.03189849424607843 10-11 0.05931538186254254 12-13 0.052065724079342894 14-15 0.05450424533369186 16-17 0.04712277559079768 18-19 0.03453473343996921 20-21 0.022737563047307972 22-23 0.0025044272341962406 24-25 0.0040532177606070734 26-27 0.0032623460024398393 28-29 0.004481606629614325 30-31 0.0019442264054944497 32-33 0.008172341501061416 34-35 0.004185029720301612 36-37 0.0065905979847269485 38-39 0.001911273415570815 40-41 0.0030316750729743962 42-43 0.003064628062898031 44-45 0.0042509357001488815 46-47 0.0043168416799961515 48-49 0.001812414445799911 50-51 0.0011863076372508506 52-53 5.931538186254253E-4 54-55 0.004679324569156133 56-57 5.602008287017906E-4 58-59 5.272478387781559E-4 60-61 6.590597984726948E-5 62-63 9.226837178617728E-4 64-65 0.0 66-67 0.0037236878613707254 68-69 7.908717581672339E-4 70-71 0.0 72-73 0.002043085375265354 74-75 0.004877042508697942 76-77 0.001384025576792659 78-79 1.3181195969453897E-4 80-81 0.0 82-83 0.005305431377705193 84-85 0.0013181195969453898 86-87 0.0 88-89 8.89730727938138E-4 90-91 5.272478387781559E-4 92-93 0.0 94-95 0.0026362391938907796 96-97 0.0011204016574035813 98-99 6.590597984726949E-4 100-101 0.0 102-103 0.001384025576792659 104-105 0.0 106-107 4.942948488545211E-4 108-109 0.002207850324883528 110-111 2.306709294654432E-4 112-113 2.9657690931271264E-4 114-115 0.0 116-117 0.0015817435163344678 118-119 0.002372615274501701 120-121 0.002306709294654432 122-123 9.226837178617728E-4 124-125 0.09892487575075148 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1517313.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.78647684767685 #Duplication Level Percentage of deduplicated Percentage of total 1 77.96755656257001 45.83437958735457 2 12.080787906561113 14.20373917141499 3 3.962507368207066 6.988255429795609 4 1.8133146794818558 4.263935256916508 5 1.0964522779350256 3.2228283225704963 6 0.6634948921620454 2.340271626998156 7 0.4612211399277832 1.8979496104816629 8 0.33249603258037935 1.5637016256984704 9 0.2535765095678557 1.3416182647942911 >10 1.2877035518773476 13.189531608563582 >50 0.05840614681393647 2.3143511249627045 >100 0.021471262410197284 2.082948049980911 >500 5.620388363283766E-4 0.2271849861783186 >1k 4.496310690627013E-4 0.5293053342897999 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2965 0.195411230247154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.15441771078215238 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 6.590597984726948E-5 0.0 3 0.0 0.0 0.0 6.590597984726948E-5 0.0 4 0.0 0.0 0.0 6.590597984726948E-5 0.0 5 0.0 0.0 0.0 6.590597984726948E-5 0.0 6 0.0 0.0 0.0 6.590597984726948E-5 0.0 7 0.0 0.0 0.0 6.590597984726948E-5 0.0 8 0.0 0.0 0.0 6.590597984726948E-5 6.590597984726948E-5 9 0.0 0.0 0.0 6.590597984726948E-5 6.590597984726948E-5 10-11 0.0 0.0 0.0 6.590597984726948E-5 6.590597984726948E-5 12-13 0.0 0.0 0.0 1.3181195969453897E-4 6.590597984726948E-5 14-15 0.0 6.590597984726948E-5 0.0 1.9771793954180845E-4 6.590597984726948E-5 16-17 0.0 1.3181195969453897E-4 0.0 1.9771793954180845E-4 1.9771793954180845E-4 18-19 0.0 1.3181195969453897E-4 0.0 1.9771793954180845E-4 1.9771793954180845E-4 20-21 0.0 1.3181195969453897E-4 0.0 1.9771793954180845E-4 1.9771793954180845E-4 22-23 0.0 1.3181195969453897E-4 0.0 4.6134185893088636E-4 2.965769093127127E-4 24-25 0.0 1.3181195969453897E-4 0.0 0.0011204016574035813 3.954358790836169E-4 26-27 0.0 1.3181195969453897E-4 0.0 0.0018453674357235455 3.954358790836169E-4 28-29 0.0 1.3181195969453897E-4 0.0 0.005700867256788811 3.954358790836169E-4 30-31 0.0 1.3181195969453897E-4 0.0 0.020892195611584424 4.283888690072516E-4 32-33 0.0 1.3181195969453897E-4 0.0 0.04758411744972857 4.6134185893088636E-4 34-35 0.0 1.3181195969453897E-4 0.0 0.08636978658984665 4.6134185893088636E-4 36-37 0.0 1.3181195969453897E-4 0.0 0.14156604471193485 4.942948488545211E-4 38-39 0.0 1.3181195969453897E-4 0.0 0.22473939127918896 5.272478387781559E-4 40-41 0.0 1.6476494961817372E-4 0.0 0.32359836105009315 5.272478387781559E-4 42-43 0.0 1.9771793954180845E-4 0.0 0.4172837114029867 5.272478387781559E-4 44-45 0.0 1.9771793954180845E-4 0.0 0.5278409925967813 5.272478387781559E-4 46-47 0.0 1.9771793954180845E-4 0.0 0.6416606197930157 6.590597984726949E-4 48-49 0.0 1.9771793954180845E-4 0.0 0.7603902424878717 7.908717581672338E-4 50-51 0.0 1.9771793954180845E-4 0.0 0.880339125809902 7.908717581672338E-4 52-53 0.0 1.9771793954180845E-4 0.0 1.005395722570096 9.226837178617727E-4 54-55 0.0 1.9771793954180845E-4 0.0 1.1562874634304194 9.885896977090422E-4 56-57 0.0 1.9771793954180845E-4 0.0 1.3166037594089026 9.885896977090422E-4 58-59 0.0 1.9771793954180845E-4 0.0 1.479029046742498 9.885896977090422E-4 60-61 0.0 1.9771793954180845E-4 0.0 1.6465290945243334 9.885896977090422E-4 62-63 0.0 1.9771793954180845E-4 0.0 1.8257933597089064 0.001021542687632677 64-65 0.0 1.9771793954180845E-4 0.0 2.0111209750394283 0.0011204016574035813 66-67 0.0 1.9771793954180845E-4 0.0 2.227556212857861 0.0011204016574035813 68-69 0.0 1.9771793954180845E-4 0.0 2.4599406977993334 0.0011204016574035813 70-71 0.0 1.9771793954180845E-4 0.0 2.702870139516369 0.0011204016574035813 72-73 0.0 1.9771793954180845E-4 0.0 2.9330138211430334 0.0013181195969453896 74-75 0.0 1.9771793954180845E-4 0.0 3.178744267003578 0.0013181195969453896 76-77 0.0 1.9771793954180845E-4 0.0 3.464973937480269 0.0013510725868690243 78-79 0.0 1.9771793954180845E-4 0.0 3.780334051049454 0.001384025576792659 80-81 0.0 1.9771793954180845E-4 0.0 4.089235378593607 0.0014169785667162938 82-83 0.0 1.9771793954180845E-4 0.0 4.404925022062027 0.0015487905264108329 84-85 0.0 1.9771793954180845E-4 0.0 4.735344652026312 0.0015817435163344676 86-87 0.0 1.9771793954180845E-4 0.0 5.113348399440326 0.0015817435163344676 88-89 0.0 1.9771793954180845E-4 0.0 5.53794767460636 0.0015817435163344676 90-91 0.0 1.9771793954180845E-4 0.0 5.968840970847808 0.0015817435163344676 92-93 0.0 1.9771793954180845E-4 0.0 6.395318566439489 0.0016476494961817371 94-95 0.0 1.9771793954180845E-4 0.0 6.831517294058642 0.0017135554760290066 96-97 0.0 1.9771793954180845E-4 0.0 7.317507989452407 0.0017135554760290066 98-99 0.0 1.9771793954180845E-4 0.0 7.867625203237566 0.0017135554760290066 100-101 0.0 1.9771793954180845E-4 0.0 8.437184681077667 0.0017135554760290066 102-103 0.0 1.9771793954180845E-4 0.0 9.009116774192272 0.0018453674357235455 104-105 0.0 2.6362391938907794E-4 0.0 9.610377028338913 0.0018453674357235455 106-107 0.0 2.6362391938907794E-4 0.0 10.265449515030848 0.0018783204256471802 108-109 0.0 2.6362391938907794E-4 0.0 10.956473713729467 0.0019442264054944497 110-111 0.0 2.6362391938907794E-4 0.0 11.701474909923002 0.002010132385341719 112-113 0.0 2.6362391938907794E-4 0.0 12.418004722822516 0.002043085375265354 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2740 0.0 46.998337 1 GTATCAA 4470 0.0 44.81373 1 GTACATG 9150 0.0 37.334713 1 ATGGGGG 5140 0.0 36.618607 5 TACATGG 9400 0.0 35.701637 2 CATGGGG 7830 0.0 35.317673 4 ACATGGG 9435 0.0 35.252148 3 TCAACGC 5720 0.0 34.592182 4 CAACGCA 5770 0.0 34.27205 5 CGTATAC 70 0.0027649293 34.06083 3 TATCAAC 6025 0.0 33.14327 2 ATCAACG 6005 0.0 32.954773 3 AACGCAG 6110 0.0 32.481712 6 TGGGGGG 3975 0.0 29.836771 6 ACGCAGA 7375 0.0 26.745998 7 CGCAGAG 7760 0.0 25.4182 8 GCAGAGT 8330 0.0 23.6789 9 GAGTACT 3655 0.0 22.558168 12-13 AGAGTAC 7835 0.0 22.01852 10-11 GTACTTT 3800 0.0 21.532215 14-15 >>END_MODULE