FastQCFastQC Report
Mon 15 Aug 2016
SRR2921583_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921583_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1711343
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG3450.094.885952
GTACGGT3250.058.6019674
TACGGTC2350.055.7175
AGTACGG5150.053.1613733
GGTATCA20450.051.811131
GTATCAA30350.044.128611
TCAACGC36250.035.4642944
GTACATG85300.034.6121861
TACATGG87450.033.962452
CAACGCA38150.033.6970675
ACATGGG89450.032.7363553
TATCAAC39900.032.668522
GTAGTAC4750.032.581881
AACGCAG40650.031.9118966
ATCAACG40600.031.8111423
CATGGGG79700.030.3935684
ATGGGGG46550.030.1734665
CTAGTAC4150.030.1208761
ACGCAGA50550.025.536167
TAGGACG2552.4716428E-823.3409272
Produced by FastQC (version 0.11.5)