##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921583_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1711343
Sequences flagged as poor quality	0
Sequence length	125
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.086265582060406	31.0	31.0	34.0	26.0	34.0
2	30.173058235549508	31.0	31.0	34.0	26.0	34.0
3	30.173187958229295	31.0	31.0	34.0	26.0	34.0
4	33.64331755819845	37.0	35.0	37.0	28.0	37.0
5	33.78667514343998	37.0	35.0	37.0	30.0	37.0
6	33.643113624796435	37.0	35.0	37.0	28.0	37.0
7	33.67435283283363	37.0	35.0	37.0	29.0	37.0
8	33.64728344931437	37.0	35.0	37.0	28.0	37.0
9	35.27440378696731	39.0	37.0	39.0	28.0	39.0
10-11	35.333339955812484	39.0	37.0	39.0	29.0	39.0
12-13	35.28972158123766	39.0	37.0	39.0	29.0	39.0
14-15	36.587727007385425	40.5	38.0	41.0	28.5	41.0
16-17	36.552457923397	40.5	38.0	41.0	28.0	41.0
18-19	36.49605193114414	40.0	38.0	41.0	27.5	41.0
20-21	36.54658271310895	40.0	38.0	41.0	28.0	41.0
22-23	36.45689993180794	40.0	38.0	41.0	27.5	41.0
24-25	36.430562721792185	40.0	38.0	41.0	27.5	41.0
26-27	36.38789418602816	40.0	38.0	41.0	27.5	41.0
28-29	36.325538188428624	40.0	38.0	41.0	27.5	41.0
30-31	36.2252868069113	40.0	38.0	41.0	27.0	41.0
32-33	36.13619069935133	40.0	37.0	41.0	27.0	41.0
34-35	36.00437901694751	40.0	37.0	41.0	26.5	41.0
36-37	35.89484632829304	40.0	37.0	41.0	26.0	41.0
38-39	35.769765032492025	40.0	37.0	41.0	25.0	41.0
40-41	35.654574506688604	40.0	36.0	41.0	25.0	41.0
42-43	35.39324290922393	40.0	35.5	41.0	24.0	41.0
44-45	35.02773289749629	40.0	35.0	41.0	23.0	41.0
46-47	34.767841689246396	39.0	35.0	41.0	22.5	41.0
48-49	34.5584952870348	39.0	35.0	41.0	21.5	41.0
50-51	34.34544214689866	39.0	34.5	41.0	20.0	41.0
52-53	34.12854290460767	38.5	34.0	40.0	19.5	41.0
54-55	33.878073244229824	38.0	34.0	40.0	18.0	41.0
56-57	33.61384947377586	38.0	34.0	40.0	16.5	41.0
58-59	33.60223754092546	38.0	34.0	40.0	16.5	41.0
60-61	33.37862427345074	37.0	34.0	40.0	14.0	41.0
62-63	33.06782246457899	37.0	33.0	40.0	12.5	41.0
64-65	32.75722225176368	36.0	33.0	39.0	9.0	41.0
66-67	32.43220295405421	35.5	33.0	39.0	7.5	41.0
68-69	32.096441216050785	35.0	33.0	38.5	6.5	40.0
70-71	31.735103074018475	35.0	32.5	37.5	2.0	40.0
72-73	31.399189116384033	35.0	32.0	37.0	2.0	39.0
74-75	31.050492507930905	35.0	32.0	36.5	2.0	39.0
76-77	30.763934816106413	35.0	32.0	36.0	2.0	38.5
78-79	30.499803078634734	35.0	31.5	35.5	2.0	37.0
80-81	30.25809758768406	35.0	31.0	35.0	2.0	37.0
82-83	30.052107321559735	35.0	31.0	35.0	2.0	36.5
84-85	29.85274080064604	35.0	31.0	35.0	2.0	36.0
86-87	29.688402617125845	35.0	31.0	35.0	2.0	36.0
88-89	29.529185265607186	34.5	31.0	35.0	2.0	35.5
90-91	29.354295719794337	34.0	31.0	35.0	2.0	35.0
92-93	29.280510978804365	34.0	30.0	35.0	2.0	35.0
94-95	29.151902920688606	34.0	30.0	35.0	2.0	35.0
96-97	29.06288336119644	34.0	30.0	35.0	2.0	35.0
98-99	28.9667445392303	34.0	30.0	35.0	2.0	35.0
100-101	28.852369162698537	34.0	30.0	35.0	2.0	35.0
102-103	27.966031940996047	33.5	28.0	34.5	2.0	35.0
104-105	28.89542102313797	34.0	30.5	35.0	2.0	35.0
106-107	29.177153557177025	35.0	31.0	35.0	2.0	35.0
108-109	29.171191865102436	35.0	31.0	35.0	2.0	35.0
110-111	29.10181915606632	35.0	31.0	35.0	2.0	35.0
112-113	29.016495524275378	35.0	31.0	35.0	2.0	35.0
114-115	28.8932978368451	35.0	31.0	35.0	2.0	35.0
116-117	28.769404204767834	35.0	30.5	35.0	2.0	35.0
118-119	28.470565807088352	34.0	30.0	35.0	2.0	35.0
120-121	28.33384482245815	34.0	30.0	35.0	2.0	35.0
122-123	28.083090590255722	34.0	30.0	35.0	2.0	35.0
124-125	27.303856970811815	33.5	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	77055.0
3	12741.0
4	6152.0
5	5599.0
6	6091.0
7	6307.0
8	6171.0
9	6654.0
10	7019.0
11	7345.0
12	7416.0
13	7419.0
14	7406.0
15	6441.0
16	5440.0
17	5055.0
18	4677.0
19	4561.0
20	4519.0
21	4727.0
22	5643.0
23	7396.0
24	10083.0
25	12378.0
26	14530.0
27	16544.0
28	19504.0
29	23643.0
30	29069.0
31	36306.0
32	47376.0
33	65714.0
34	101986.0
35	171062.0
36	340103.0
37	492122.0
38	119040.0
39	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.42754723134842	17.0548960860653	11.676457884126771	25.841098798459512
2	17.01920385327674	19.016086682363557	38.082840540987526	25.881868923372174
3	20.132621313379946	23.409000063114046	27.830898767406037	28.627479856099974
4	14.788421675784246	14.717183576316225	33.39313671462041	37.10125803327912
5	15.278879258199934	36.64256689192388	32.734277336958336	15.344276512917856
6	35.00374321542205	33.70747941231518	16.19712708216358	15.091650290099196
7	30.713273826124123	31.114880251826932	18.803822072392055	19.36802384965689
8	28.913644726065474	33.62621764753682	18.48000640720975	18.980131219187957
9	28.15260408051617	15.665221627542286	17.958152118958846	38.2240221729827
10-11	27.10595600032855	24.8261143951704	25.789029225058467	22.27890037944258
12-13	26.85403797490547	22.416648647385063	26.150876905904287	24.578436471805183
14-15	24.572052970919998	23.808420241732282	23.261018609492893	28.358508177854823
16-17	25.219291003172494	26.722361939387916	23.926531339916032	24.13181571752355
18-19	25.043915208136163	26.0125341749465	25.270988254398972	23.672562362518363
20-21	25.65182092525343	24.862832223073646	25.28621553708922	24.19913131458371
22-23	26.026380319685284	24.412785073169875	24.822811114455984	24.738023492688857
24-25	25.377636352690114	25.038475274412146	24.85984946567152	24.72403890722622
26-27	24.907043203682434	25.267646299718844	25.208953666433516	24.616356830165206
28-29	25.022630452097538	25.327604474939896	25.1825415963347	24.467223476627865
30-31	25.408129837057835	24.948062312950537	25.05542991020457	24.58837793978706
32-33	24.943332990428274	25.11152207763059	25.053043126545994	24.892101805395146
34-35	25.092187934779815	25.39885750095286	25.047643098824064	24.461311465443263
36-37	25.238605045827594	25.317380358532866	24.961576581525435	24.482438014114106
38-39	25.090896020563157	25.4220141073663	25.07707386408466	24.410016007985874
40-41	25.064112269396045	25.232993369268552	25.08819638609645	24.614697975238954
42-43	25.091993309152453	25.039187747005542	25.023553560094307	24.84526538374769
44-45	24.95890015469789	25.115088333254437	25.15824828455996	24.767763227487716
46-47	24.9477561671978	25.165381575044528	25.13440494924655	24.75245730851112
48-49	24.803376040400263	25.452895585897316	25.089106190966053	24.654622182736368
50-51	24.814658734769694	25.412531898453327	25.141650758841262	24.631158607935717
52-53	25.04421504702634	25.188461889142676	25.062859594285303	24.704463469545683
54-55	24.827527156728777	25.05651685511463	25.07282316594915	25.043132822207443
56-57	24.843624338376944	25.31961887706436	24.93123679091776	24.905519993640937
58-59	24.80395239453346	25.30732339643637	25.114957710523978	24.77376649850619
60-61	24.981954817786033	25.2216708173021	25.25589091183256	24.54048345307931
62-63	24.854134070996363	25.27450497928391	25.202762846517423	24.6685981032023
64-65	24.78426598418837	25.071083371955545	25.17730662254481	24.967344021311277
66-67	24.82395230580396	25.32859898299141	24.937898182243266	24.909550528961365
68-69	24.730150260213556	25.385177134871828	25.043797525246227	24.840875079668393
70-71	24.60533061617237	25.263312656486665	25.232124933283995	24.89923179405697
72-73	24.813630527012833	25.436839524413745	25.05834365391123	24.69118629466219
74-75	24.732219297040956	25.389771475489354	25.05865783852805	24.819351388941648
76-77	24.75796214823715	25.212261614777898	24.99593827581854	25.033837961166412
78-79	24.75113906316712	25.302851551963517	25.052726694099842	24.89328269076952
80-81	24.77546675300744	25.477404364981844	25.009913506301253	24.73721537570947
82-83	24.765306098593065	25.392676124169466	25.127911244733923	24.71410653250354
84-85	24.81242267676741	25.395652425458504	24.879979148659885	24.9119457491142
86-87	24.707244076357032	25.494179947366174	24.976462055547795	24.822113920728995
88-89	24.6740475959476	25.355467549120796	24.981414225481682	24.98907062944992
90-91	24.741539581892898	25.27879943681959	25.039874412403996	24.939786568883523
92-93	24.744414878152757	25.282890194454776	25.18456855793862	24.78812636945385
94-95	24.83902732649197	25.12155496855934	25.184144089389655	24.855273615559035
96-97	24.68740274938892	25.169897274133856	25.096494521822855	25.04620545465437
98-99	24.60821664688088	25.236618591546332	24.980343264598798	25.174821496973987
100-101	24.58469033007966	25.350851715732826	25.171035645891493	24.893422308296024
102-103	24.344813098215877	25.220177918776308	25.36541952501817	25.06958945798965
104-105	24.293947907876927	25.36011140225189	25.230062245949807	25.115878443921382
106-107	24.431210434682	25.303572101606264	25.168735572272233	25.096481891439502
108-109	24.390041496200883	25.311805918987	25.236796590304422	25.061355994507693
110-111	24.24228966644822	25.643281355044795	25.181884079778023	24.932544898728967
112-113	24.168187190203984	25.749638799572843	25.238129325092075	24.844044685131106
114-115	24.18213833008306	25.739814240689974	25.2259752130014	24.85207221622557
116-117	24.11090314207259	25.718871310538034	25.177661593014268	24.992563954375115
118-119	24.18686389391779	25.642453042746798	25.165753456364993	25.00492960697042
120-121	24.154311739187346	25.86359832831579	25.156165270480003	24.825924662016867
122-123	24.064087221356097	25.922765063775184	25.16770640743658	24.84544130743214
124-125	24.014938882127975	25.86497675105693	25.210242900455132	24.909841466359964
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	729.0
1	589.0
2	396.0
3	293.5
4	210.5
5	167.5
6	152.0
7	178.0
8	224.5
9	243.0
10	243.0
11	240.5
12	277.0
13	315.5
14	324.5
15	354.0
16	395.0
17	439.0
18	493.5
19	561.5
20	651.5
21	716.5
22	819.0
23	979.0
24	1174.0
25	1457.5
26	1881.5
27	2514.0
28	3425.5
29	4611.5
30	6146.0
31	8175.0
32	10825.5
33	13086.5
34	15276.5
35	18056.5
36	21387.0
37	24930.0
38	28238.0
39	31997.5
40	35766.0
41	38874.5
42	42263.5
43	47198.5
44	52309.0
45	56919.5
46	61514.0
47	65722.0
48	70168.5
49	74276.0
50	76223.5
51	77011.0
52	76847.0
53	76561.5
54	76861.0
55	75302.5
56	70996.5
57	65352.0
58	60525.5
59	55273.5
60	48149.5
61	41570.5
62	35371.5
63	28002.0
64	21285.5
65	16597.5
66	13121.5
67	10436.0
68	8328.5
69	6483.0
70	5200.0
71	4078.5
72	3106.5
73	2596.0
74	2149.5
75	1693.0
76	1268.0
77	851.0
78	585.0
79	389.0
80	252.5
81	192.0
82	164.0
83	137.0
84	100.0
85	75.0
86	69.0
87	59.0
88	44.0
89	32.0
90	28.5
91	28.5
92	23.5
93	22.0
94	20.5
95	13.0
96	12.5
97	11.5
98	7.5
99	5.5
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026645739632557588
2	0.010284320559934508
3	0.009057214129487776
4	0.010518055118114837
5	0.015309613560811597
6	0.09250045139986549
7	0.05621316124236929
8	0.04499390244971347
9	0.025243332283475608
10-11	0.04718516393265406
12-13	0.012446365223102556
14-15	0.025827668678926434
16-17	0.03187555037184247
18-19	0.0385662020997544
20-21	0.02816501426072973
22-23	0.0349433164479593
24-25	0.029450554330721544
26-27	0.04303637552495321
28-29	0.04122493269905565
30-31	0.02328580535871535
32-33	0.027522244225733822
34-35	0.04131258315837328
36-37	0.008589745013127117
38-39	0.01881563193351654
40-41	0.0392674057742954
42-43	0.02039334020123377
44-45	0.015543348118991926
46-47	0.021941831649178452
48-49	0.027054775109373163
50-51	0.02600296959756168
52-53	0.022613818503946902
54-55	0.02039334020123377
56-57	0.02331502217848789
58-59	0.016302985433077996
60-61	0.021036110236229675
62-63	0.02068550839895918
64-65	0.01893249921260671
66-67	0.025886102318471514
68-69	0.020130388823280897
70-71	0.04289029142609051
72-73	0.021036110236229675
74-75	0.008648178652672199
76-77	0.014433108967635359
78-79	0.023227371719170265
80-81	0.017150273206481693
82-83	0.02284755306212723
84-85	0.010634922397205001
86-87	0.01612768451444275
88-89	0.020860809317594427
90-91	0.020218039282598518
92-93	0.007158120844272598
94-95	0.010693356036750085
96-97	0.013761122112866911
98-99	0.005697279855645537
100-101	0.008765045931762364
102-103	0.0033015006342971575
104-105	0.003915053849520523
106-107	6.719868547684479E-4
108-109	0.01300148479878084
110-111	0.0011394559711291072
112-113	0.0020743942038504264
114-115	0.011920462467196816
116-117	0.004908425721786925
118-119	0.2815040585084346
120-121	8.765045931762364E-4
122-123	0.0011102391513565662
124-125	0.31898923827660497
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
125	1711343.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.73145187255705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.96661262041907	51.601197755022085
2	10.440916859487873	13.30829580671642
3	3.4707707199581987	6.635917712990884
4	1.590196853078015	4.053822168393328
5	0.9058891391755126	2.886681503761816
6	0.5768084037643353	2.205650221451592
7	0.4100037918644151	1.8291095850141013
8	0.29311833492912637	1.4944685644399742
9	0.20866517354198702	1.1968681018562919
>10	1.0861593262857692	11.98054057297935
>50	0.04068625048517271	1.7081746268111129
>100	0.009989290143691069	1.019337802183093
>500	1.8323686672481473E-4	0.07993557837994741
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1686727909016486E-4
2	0.0	0.0	0.0	0.0	1.1686727909016486E-4
3	0.0	0.0	0.0	0.0	1.1686727909016486E-4
4	0.0	0.0	0.0	0.0	1.1686727909016486E-4
5	0.0	0.0	0.0	0.0	1.7530091863524728E-4
6	0.0	0.0	0.0	0.0	2.337345581803297E-4
7	0.0	0.0	0.0	0.0	2.337345581803297E-4
8	0.0	0.0	0.0	0.0	2.337345581803297E-4
9	0.0	0.0	0.0	0.0	2.337345581803297E-4
10-11	0.0	0.0	0.0	0.0	2.337345581803297E-4
12-13	0.0	0.0	0.0	2.9216819772541214E-5	3.213850174979534E-4
14-15	0.0	0.0	0.0	5.843363954508243E-5	5.551195756782831E-4
16-17	0.0	0.0	0.0	5.843363954508243E-5	7.012036745409891E-4
18-19	0.0	0.0	0.0	8.765045931762364E-5	7.012036745409891E-4
20-21	0.0	0.0	0.0	1.4608409886270607E-4	7.012036745409891E-4
22-23	0.0	0.0	0.0	3.213850174979534E-4	8.765045931762364E-4
24-25	0.0	0.0	0.0	8.472877734036952E-4	0.0010518055118114837
26-27	0.0	0.0	0.0	0.0015484914479446843	0.0011102391513565662
28-29	0.0	0.0	0.0	0.004411739785653723	0.0011102391513565662
30-31	0.0	0.0	0.0	0.017237923665799317	0.0011394559711291074
32-33	0.0	0.0	0.0	0.04201378683291426	0.001227106430446731
34-35	2.9216819772541214E-5	0.0	0.0	0.07505800999565838	0.0012563232502192721
36-37	1.1686727909016486E-4	0.0	0.0	0.11826968643924683	0.0012855400699918135
38-39	1.7530091863524728E-4	0.0	0.0	0.1785732024497719	0.0013439737095368959
40-41	2.337345581803297E-4	0.0	0.0	0.24638544114184008	0.0013439737095368959
42-43	2.337345581803297E-4	0.0	0.0	0.3192814064743304	0.001373190529309437
44-45	2.337345581803297E-4	0.0	0.0	0.4089770431760319	0.0014316241688545196
46-47	2.337345581803297E-4	0.0	0.0	0.5086940490597152	0.0014608409886270608
48-49	2.337345581803297E-4	0.0	0.0	0.6016619695759412	0.0015192746281721431
50-51	2.337345581803297E-4	0.0	0.0	0.6908317035217371	0.0015192746281721431
52-53	2.337345581803297E-4	0.0	0.0	0.7871011246722603	0.0015484914479446843
54-55	2.337345581803297E-4	0.0	0.0	0.9040268374019702	0.001636141907262308
56-57	2.337345581803297E-4	0.0	0.0	1.0419302267283648	0.001636141907262308
58-59	2.337345581803297E-4	0.0	0.0	1.1744285043968392	0.001636141907262308
60-61	2.337345581803297E-4	0.0	0.0	1.2993304089244528	0.001636141907262308
62-63	2.337345581803297E-4	0.0	0.0	1.4256931544406937	0.0016945755468073904
64-65	2.337345581803297E-4	0.0	0.0	1.5727998419954385	0.0016945755468073904
66-67	2.337345581803297E-4	0.0	0.0	1.7536811732072413	0.0017237923665799318
68-69	2.337345581803297E-4	0.0	0.0	1.9416037579842262	0.001753009186352473
70-71	2.337345581803297E-4	0.0	0.0	2.1270429130805457	0.0017822260061250142
72-73	2.9216819772541215E-4	0.0	0.0	2.3068724387805366	0.0018698764654426377
74-75	2.9216819772541215E-4	0.0	0.0	2.5050793441174566	0.0018698764654426377
76-77	2.9216819772541215E-4	0.0	0.0	2.754094299038825	0.0018698764654426377
78-79	2.9216819772541215E-4	0.0	0.0	3.025839939743231	0.0018698764654426377
80-81	2.9216819772541215E-4	0.0	0.0	3.284467228369766	0.0018990932852151789
82-83	2.9216819772541215E-4	0.0	0.0	3.5409909059726776	0.002045177384077885
84-85	2.9216819772541215E-4	0.0	0.0	3.8229916504172454	0.002045177384077885
86-87	2.9216819772541215E-4	0.0	0.0	4.145662207985191	0.0020743942038504264
88-89	2.9216819772541215E-4	0.0	0.0	4.513355884822622	0.0021036110236229673
90-91	2.9216819772541215E-4	0.0	0.0	4.884643230492076	0.0021036110236229673
92-93	2.9216819772541215E-4	0.0	0.0	5.242227887688207	0.0023081287620307558
94-95	3.5060183727049455E-4	0.0	0.0	5.615735711660374	0.0024249960411209205
96-97	3.5060183727049455E-4	0.0	0.0	6.036633217303603	0.0024834296806660033
98-99	3.5060183727049455E-4	0.0	0.0	6.532150480645902	0.0025126465004385447
100-101	3.5060183727049455E-4	0.0	0.0	7.045285486311044	0.0025126465004385447
102-103	3.5060183727049455E-4	0.0	0.0	7.543578347531733	0.002571080139983627
104-105	3.5060183727049455E-4	0.0	0.0	8.059488951075267	0.002600296959756168
106-107	3.5060183727049455E-4	0.0	0.0	8.619721470213744	0.0026587305993012508
108-109	3.5060183727049455E-4	0.0	0.0	9.242974669601594	0.0027755978783914155
110-111	3.5060183727049455E-4	0.0	0.0	9.908650691299172	0.00289246515748158
112-113	3.5060183727049455E-4	0.0	0.0	10.548382177038736	0.0029216819772541216
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTACG	345	0.0	94.88595	2
GTACGGT	325	0.0	58.601967	4
TACGGTC	235	0.0	55.717	5
AGTACGG	515	0.0	53.161373	3
GGTATCA	2045	0.0	51.81113	1
GTATCAA	3035	0.0	44.12861	1
TCAACGC	3625	0.0	35.464294	4
GTACATG	8530	0.0	34.612186	1
TACATGG	8745	0.0	33.96245	2
CAACGCA	3815	0.0	33.697067	5
ACATGGG	8945	0.0	32.736355	3
TATCAAC	3990	0.0	32.66852	2
GTAGTAC	475	0.0	32.58188	1
AACGCAG	4065	0.0	31.911896	6
ATCAACG	4060	0.0	31.811142	3
CATGGGG	7970	0.0	30.393568	4
ATGGGGG	4655	0.0	30.173466	5
CTAGTAC	415	0.0	30.120876	1
ACGCAGA	5055	0.0	25.53616	7
TAGGACG	255	2.4716428E-8	23.340927	2
>>END_MODULE